LOCUS       sequence-455606-       10129 bp    DNA     circular SYN 15-APR-2025
DEFINITION  aaRS/tRNA system for incorporation of 5HTP within proteins in 
            mammalian cells at TGA codon.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10129)
  AUTHORS   Osgood AO, Zheng Y, Roy SJS, Biris N, Hussain M, Loynd C, Jewel D, 
            Italia JS, Chatterjee A
  TITLE     An Efficient Opal-Suppressor Tryptophanyl Pair Creates New Routes 
            for Simultaneously Incorporating up to Three Distinct Noncanonical 
            Amino Acids into Proteins in Mammalian Cells.
  JOURNAL   Angew Chem Int Ed Engl. 2023 May 2;62(19):e202219269. doi: 
            10.1002/anie.202219269. Epub 2023 Apr 12.
  PUBMED    36905325
REFERENCE   2  (bases 1 to 10129)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Angew Chem 
            Int Ed Engl."; date: "2023-05-2"; volume: "62(19):e202219269. doi"; 
            pages: " 10.1002/anie.202219269. Epub 2023 Apr 12"
COMMENT     Sequence Label: pAcBac1-EcTrp-TGA
FEATURES             Location/Qualifiers
     source          1..10129
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      1..589
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     490..509
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     743..760
                     /label=L4440
                     /note="L4440 vector, forward primer"
     mobile_element  894..1118
                     /mobile_element_type="transposon:Tn7"
                     /label=Tn7R
                     /note="mini-Tn7 element (right end of the Tn7 transposon)"
     CDS             complement(1185..1718)
                     /codon_start=1
                     /gene="aacC1"
                     /product="gentamycin acetyltransferase"
                     /label=GmR
                     /note="confers resistance to gentamycin"
                     /translation="MLRSSNDVTQQGSRPKTKLGGSSMGIIRTCRLGPDQVKSMRAALD
                     LFGREFGDVATYSQHQPDSDYLGNLLRSKTFIALAAFDQEAVVGALAAYVLPKFEQPRS
                     EIYIYDLAVSGEHRRQGIATALINLLKHEANALGAYVIYVQADYGDDPAVALYTKLGIR
                     EEVMHFDIDPSTAT"
     promoter        complement(join(1907..1912,1913..1929,1930..1935))
                     /gene="intI1 (promoter lies within the coding sequence)"
                     /label=Pc promoter
                     /note="class 1 integron promoter"
     polyA_signal    2365..2589
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     2578..2595
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     primer_bind     complement(3689..3708)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(3690..3708)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     promoter        complement(3753..3956)
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     complement(3782..3802)
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     enhancer        3957..4260
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        4334..4582
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for human U6 snRNA 
                     (Domitrovich & Kunkel, 2003)"
     promoter        4682..4930
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for human U6 snRNA 
                     (Domitrovich & Kunkel, 2003)"
     promoter        5030..5278
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for human U6 snRNA 
                     (Domitrovich & Kunkel, 2003)"
     promoter        5378..5626
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for human U6 snRNA 
                     (Domitrovich & Kunkel, 2003)"
     promoter        5823..5914
                     /gene="polh from Autographa californica"
                     /label=polyhedrin promoter
                     /note="promoter for the baculovirus polyhedrin gene"
     primer_bind     5839..5856
                     /label=Polyhedrin-F
                     /note="Polyhedrin promoter, forward primer"
     CDS             join(5973..6020,6021..7508)
                     /codon_start=1
                     /product="vesicular stomatitis virus G glycoprotein"
                     /label=VSV-G
                     /note="Indiana strain"
                     /translation="MKCLLYLAFLFIGVNCKFTIVFPHNQKGNWKNVPSNYHYCPSSSD
                     LNWHNDLIGTALQVKMPKSHKAIQADGWMCHASKWVTTCDFRWYGPKYITHSIRSFTPS
                     VEQCKESIEQTKQGTWLNPGFPPQSCGYATVTDAEAVIVQVTPHHVLVDEYTGEWVDSQ
                     FINGKCSNYICPTVHNSTTWHSDYKVKGLCDSNLISMDITFFSEDGELSSLGKEGTGFR
                     SNYFAYETGGKACKMQYCKHWGVRLPSGVWFEMADKDLFAAARFPECPEGSSISAPSQT
                     SVDVSLIQDVERILDYSLCQETWSKIRAGLPISPVDLSYLAPKNPGTGPAFTIINGTLK
                     YFETRYIRVDIAAPILSRMVGMISGTTTERELWDDWAPYEDVEIGPNGVLRTSSGYKFP
                     LYMIGHGMLDSDLHLSSKAQVFEHPHIQDAASQLPDDESLFFGDTGLSKNPIELVEGWF
                     SSWKSSIASFFFIIGLIIGLFLVLRVGIHLCIKLKHTKKRQIYTDIEMNRLGK"
     polyA_signal    7654..7788
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7691..7710)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     7745..7764
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     promoter        complement(7892..8140)
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for human U6 snRNA 
                     (Domitrovich & Kunkel, 2003)"
     mobile_element  8165..8330
                     /mobile_element_type="transposon:Tn7"
                     /label=Tn7L
                     /note="mini-Tn7 element (left end of the Tn7 transposon)"
     rep_origin      8514..8968
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(8601..8620)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     8810..8831
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        8994..9098
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(9099..9167,9168..9959)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(9317..9336)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
ORIGIN
        1 ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc
       61 agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt
      121 cagcagagcg cagataccaa atactgttct tctagtgtag ccgtagttag gccaccactt
      181 caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc
      241 tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa
      301 ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac
      361 ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg
      421 gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga
      481 gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact
      541 tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa
      601 cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc
      661 gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg
      721 ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag agcgcctgat
      781 gcggtatttt ctccttacgc atctgtgcgg tatttcacac cgcatagacc agccgcgtaa
      841 cctggcaaaa tcggttacgg ttgagtaata aatggatgcc ctgcgtaagc gggtgtgggc
      901 ggacaataaa gtcttaaact gaacaaaata gatctaaact atgacaataa agtcttaaac
      961 tagacagaat agttgtaaac tgaaatcagt ccagttatgc tgtgaaaaag catactggac
     1021 ttttgttatg gctaaagcaa actcttcatt ttctgaagtg caaattgccc gtcgtattaa
     1081 agaggggcgt ggccaagggc atggtaaaga ctatattcgc ggcgttgtga caatttaccg
     1141 aacaactccg cggccgggaa gccgatctcg gcttgaacga attgttaggt ggcggtactt
     1201 gggtcgatat caaagtgcat cacttcttcc cgtatgccca actttgtata gagagccact
     1261 gcgggatcgt caccgtaatc tgcttgcacg tagatcacat aagcaccaag cgcgttggcc
     1321 tcatgcttga ggagattgat gagcgcggtg gcaatgccct gcctccggtg ctcgccggag
     1381 actgcgagat catagatata gatctcacta cgcggctgct caaacttggg cagaacgtaa
     1441 gccgcgagag cgccaacaac cgcttcttgg tcgaaggcag caagcgcgat gaatgtctta
     1501 ctacggagca agttcccgag gtaatcggag tccggctgat gttgggagta ggtggctacg
     1561 tctccgaact cacgaccgaa aagatcaaga gcagcccgca tggatttgac ttggtcaggg
     1621 ccgagcctac atgtgcgaat gatgcccata cttgagccac ctaactttgt tttagggcga
     1681 ctgccctgct gcgtaacatc gttgctgctg cgtaacatcg ttgctgctcc ataacatcaa
     1741 acatcgaccc acggcgtaac gcgcttgctg cttggatgcc cgaggcatag actgtacaaa
     1801 aaaacagtca taacaagcca tgaaaaccgc cactgcgccg ttaccaccgc tgcgttcggt
     1861 caaggttctg gaccagttgc gtgagcgcat acgctacttg cattacagtt tacgaaccga
     1921 acaggcttat gtcaactggg ttcgtgcctt catccgtttc cacggtgtgc gtcacccggc
     1981 aaccttgggc agcagcgaag tcgaggcatt tctgtcctgg ctggcgaacg agcgcaaggt
     2041 ttcggtctcc acgcatcgtc aggcattggc ggccttgctg ttcttctacg gcaaggtgct
     2101 gtgcacggat ctgcccttgc ttcaggagat cggtagacct cggccgtcgc ggcgcttgcc
     2161 ggtggtgctg accccggatg aagtggttcg catcctcggt tttctggaag gcgagcatcg
     2221 tttgttcgcc caggactcta gctatagttc tagtggttgg ctacgtaccc gtagtggcta
     2281 tggcagggct tgcgcttaat gcgccgctac agggcgcgtg gggatacccc ctagagcccc
     2341 agctggttct ttccgcctca gaagccatag agcccaccgc atccccagca tgcctgctat
     2401 tgtcttccca atcctccccc ttgctgtcct gccccacccc accccccaga atagaatgac
     2461 acctactcag acaatgcgat gcaatttcct cattttatta ggaaaggaca gtgggagtgg
     2521 caccttccag ggtcaaggaa ggcacggggg aggggcaaac aacagatggc tggcaactag
     2581 aaggcacagt cgaggctgat cagcgggttt aaacgggccc tctagactcg agttaaagtc
     2641 gacttaacgc gttgaattct tacggcttcg ccacaaaacc aatcgcttcg tacaccgctt
     2701 ttagcgtacg ggaagcgtgc gcgctggctt tttccgcgcc atctttcatc acctgttgca
     2761 ggaaggcttc atcgttgcgg aaacggtgat agcgttcctg caattcagtc agcataccgg
     2821 aaacggcatc agccacttca cctttcagat gaccatacat cttgccttcg aactgttttt
     2881 ccagttctgg gatgctctgg cccgttaccg ctgaaaggat atccaacagg ttggaaacgc
     2941 ccgctttgtt ctgcacatcg tagcgaacta ccggcggctc gtcggagtca gtgaccgcac
     3001 gtttgatttt cttcactacc gatttcggat cttccagcag gccgataacg ttattgcgat
     3061 tatcgtcaga cttggacatc ttcttggtcg gctccagcag cgacattacg cgcgcgccag
     3121 atttcggaat aaacggctcc ggcaccttaa agatctcgcc atacagcgcg ttgaaacgct
     3181 gggcaatatc gcggctcagt tcgaggtgct gtttctggtc ttcaccacaa ggacccagat
     3241 tagtttgata cagcaggatg tccgctgcca tcagcaccgg atagtcaaac agaccagcgt
     3301 tgatgttctc ggcataacgc gcagatttat ctttaaactg cgtcatgcga ctcagttcgc
     3361 cgaagtaggt atagcagttc agtgcccagc ctaactgtgc atgttccggc acgtgggact
     3421 gaacaaaaat ggtgcttttc tcaggatcga taccacaagc cagatacaag gccagcgtat
     3481 ccagcgtcgc tttacgcagc ttctgtgcat cctggcgcac ggtgatcgcg tgttggtcaa
     3541 cgatacagta aatgcaatgg tagtcatcct gcatgtttac ccactgacgc agcgcaccca
     3601 tgtagttacc aatggtcaat tcacctgagg gctgtgcgcc agcaaaaacg atgggcttag
     3661 tcatggtggc gctagccagc ttgggtctcc ctatagtgag tcgtattaat ttcgataagc
     3721 cagtaagcag tgggttctct agttagccag agagctctgc ttatatagac ctcccaccgt
     3781 acacgcctac cgcccatttg cgtcaatggg gcggagttgt tacgacattt tggaaagtcc
     3841 cgttgatttt ggtgccaaaa caaactccca ttgacgtcaa tggggtggag acttggaaat
     3901 ccccgtgagt caaaccgcta tccacgccca ttgatgtact gccaaaaccg catcaccatg
     3961 gtaatagcga tgactaatac gtagatgtac tgccaagtag gaaagtccca taaggtcatg
     4021 tactgggcat aatgccaggc gggccattta ccgtcattga cgtcaatagg gggcgtactt
     4081 ggcatatgat acacttgatg tactgccaag tgggcagttt accgtaaata ctccacccat
     4141 tgacgtcaat ggaaagtccc tattggcgtt actatgggaa catacgtcat tattgacgtc
     4201 aatgggcggg ggtcgttggg cggtcagcca ggcgggccat ttaccgtaag ttatgtaacg
     4261 cggaactcca tatatgggct atgaactaat gaccccgtaa ttgattacta ttaataacta
     4321 ggtcgggcag gaagagggcc tatttcccat gattccttca tatttgcata tacgatacaa
     4381 ggctgttaga gagataatta gaattaattt gactgtaaac acaaagatat tagtacaaaa
     4441 tacgtgacgt agaaagtaat aatttcttgg gtagtttgca gttttaaaat tatgttttaa
     4501 aatggactat catatgctta ccgtaacttg aaagtatttc gatttcttgg ctttatatat
     4561 cttgtggaaa ggacgaaaca ccaggggcgt agttcaattg gtagagcacc ggtcttcaaa
     4621 accgggtgtt gggagttcga gtctctccgc ccctgccatt ttttgctagg tcgggcagga
     4681 agagggccta tttcccatga ttccttcata tttgcatata cgatacaagg ctgttagaga
     4741 gataattaga attaatttga ctgtaaacac aaagatatta gtacaaaata cgtgacgtag
     4801 aaagtaataa tttcttgggt agtttgcagt tttaaaatta tgttttaaaa tggactatca
     4861 tatgcttacc gtaacttgaa agtatttcga tttcttggct ttatatatct tgtggaaagg
     4921 acgaaacacc aggggcgtag ttcaattggt agagcaccgg tcttcaaaac cgggtgttgg
     4981 gagttcgagt ctctccgccc ctgccatttt ttgctaggtc gggcaggaag agggcctatt
     5041 tcccatgatt ccttcatatt tgcatatacg atacaaggct gttagagaga taattagaat
     5101 taatttgact gtaaacacaa agatattagt acaaaatacg tgacgtagaa agtaataatt
     5161 tcttgggtag tttgcagttt taaaattatg ttttaaaatg gactatcata tgcttaccgt
     5221 aacttgaaag tatttcgatt tcttggcttt atatatcttg tggaaaggac gaaacaccag
     5281 gggcgtagtt caattggtag agcaccggtc ttcaaaaccg ggtgttggga gttcgagtct
     5341 ctccgcccct gccatttttt gctaggtcgg gcaggaagag ggcctatttc ccatgattcc
     5401 ttcatatttg catatacgat acaaggctgt tagagagata attagaatta atttgactgt
     5461 aaacacaaag atattagtac aaaatacgtg acgtagaaag taataatttc ttgggtagtt
     5521 tgcagtttta aaattatgtt ttaaaatgga ctatcatatg cttaccgtaa cttgaaagta
     5581 tttcgatttc ttggctttat atatcttgtg gaaaggacga aacaccaggg gcgtagttca
     5641 attggtagag caccggtctt caaaaccggg tgttgggagt tcgagtctct ccgcccctgc
     5701 cattttttgc tagtcaataa tcaatgtcaa cgcgtatatc tggcccgtac atcgcgaagc
     5761 agcgcaaaac ggatcctgca ggtatttgcg gccgcggtcc gtatactccg gaatattaat
     5821 agatcatgga gataattaaa atgataacca tctcgcaaat aaataagtat tttactgttt
     5881 tcgtaacagt tttgtaataa aaaaacctat aaatattccg gattattcat accgtcccac
     5941 catcgggcgc gaactcctaa aaaaccgcca ccatgaagtg ccttttgtac ttagcctttt
     6001 tattcattgg ggtgaattgc aagttcacca tagtttttcc acacaaccaa aaaggaaact
     6061 ggaaaaatgt tccttctaat taccattatt gcccgtcaag ctcagattta aattggcata
     6121 atgacttaat aggcacagcc ttacaagtca aaatgcccaa gagtcacaag gctattcaag
     6181 cagacggttg gatgtgtcat gcttccaaat gggtcactac ttgtgatttc cgctggtatg
     6241 gaccgaagta tataacacat tccatccgat ccttcactcc atctgtagaa caatgcaagg
     6301 aaagcattga acaaacgaaa caaggaactt ggctgaatcc aggcttccct cctcaaagtt
     6361 gtggatatgc aactgtgacg gatgccgaag cagtgattgt ccaggtgact cctcaccatg
     6421 tgctggttga tgaatacaca ggagaatggg ttgattcaca gttcatcaac ggaaaatgca
     6481 gcaattacat atgccccact gtccataact ctacaacctg gcattctgac tataaggtca
     6541 aagggctatg tgattctaac ctcatttcca tggacatcac cttcttctca gaggacggag
     6601 agctatcatc cctgggaaag gagggcacag ggttcagaag taactacttt gcttatgaaa
     6661 ctggaggcaa ggcctgcaaa atgcaatact gcaagcattg gggagtcaga ctcccatcag
     6721 gtgtctggtt cgagatggct gataaggatc tctttgctgc agccagattc cctgaatgcc
     6781 cagaagggtc aagtatctct gctccatctc agacctcagt ggatgtaagt ctaattcagg
     6841 acgttgagag gatcttggat tattccctct gccaagaaac ctggagcaaa atcagagcgg
     6901 gtcttccaat ctctccagtg gatctcagct atcttgctcc taaaaaccca ggaaccggtc
     6961 ctgctttcac cataatcaat ggtaccctaa aatactttga gaccagatac atcagagtcg
     7021 atattgctgc tccaatcctc tcaagaatgg tcggaatgat cagtggaact accacagaaa
     7081 gggaactgtg ggatgactgg gcaccatatg aagacgtgga aattggaccc aatggagttc
     7141 tgaggaccag ttcaggatat aagtttcctt tatacatgat tggacatggt atgttggact
     7201 ccgatcttca tcttagctca aaggctcagg tgttcgaaca tcctcacatt caagacgctg
     7261 cttcgcaact tcctgatgat gagagtttat tttttggtga tactgggcta tccaaaaatc
     7321 caatcgagct tgtagaaggt tggttcagta gttggaaaag ctctattgcc tcttttttct
     7381 ttatcatagg gttaatcatt ggactattct tggttctccg agttggtatc catctttgca
     7441 ttaaattaaa gcacaccaag aaaagacaga tttatacaga catagagatg aaccgacttg
     7501 gaaagtgata aggccaggcc ggccaagctt gtcgagaagt actagaggat cataatcagc
     7561 cataccacat ttgtagaggt tttacttgct ttaaaaaacc tcccacacct ccccctgaac
     7621 ctgaaacata aaatgaatgc aattgttgtt gttaacttgt ttattgcagc ttataatggt
     7681 tacaaataaa gcaatagcat cacaaatttc acaaataaag catttttttc actgcattct
     7741 agttgtggtt tgtccaaact catcaatgta tcttatcatg tctggatctg atcactgctt
     7801 gagcctagca aaaaatggca ggggcggaga gactcgaact cccaacaccc ggttttgaag
     7861 accggtgctc taccaattga actacgcccc tggtgtttcg tcctttccac aagatatata
     7921 aagccaagaa atcgaaatac tttcaagtta cggtaagcat atgatagtcc attttaaaac
     7981 ataattttaa aactgcaaac tacccaagaa attattactt tctacgtcac gtattttgta
     8041 ctaatatctt tgtgtttaca gtcaaattaa ttctaattat ctctctaaca gccttgtatc
     8101 gtatatgcaa atatgaagga atcatgggaa ataggccctc ttcctgcccg acctaggaga
     8161 tccgaaccag ataagtgaaa tctagttcca aactattttg tcatttttaa ttttcgtatt
     8221 agcttacgac gctacaccca gttcccatct attttgtcac tcttccctaa ataatcctta
     8281 aaaactccat ttccacccct cccagttccc aactattttg tccgcccaca gcggggcatt
     8341 tttcttcctg ttatgttttt aatcaaacat cctgccaact ccatgtgaca aaccgtcatc
     8401 ttcggctact ttttctctgt cacagaatga aaatttttct gtcatctctt cgttattaat
     8461 gtttgtaatt gactgaatat caacgcttat ttgcagcctg aatggcgaat gggacgcgcc
     8521 ctgtagcggc gcattaagcg cggcgggtgt ggtggttacg cgcagcgtga ccgctacact
     8581 tgccagcgcc ctagcgcccg ctcctttcgc tttcttccct tcctttctcg ccacgttcgc
     8641 cggctttccc cgtcaagctc taaatcgggg gctcccttta gggttccgat ttagtgcttt
     8701 acggcacctc gaccccaaaa aacttgatta gggtgatggt tcacgtagtg ggccatcgcc
     8761 ctgatagacg gtttttcgcc ctttgacgtt ggagtccacg ttcttaatag tggactcttg
     8821 ttccaaactg gaacaacact caaccctatc tcggtctatt cttttgattt ataagggatt
     8881 ttgccgattt cggcctattg gttaaaaaat gagctgattt aacaaaaatt taacgcgaat
     8941 tttaacaaaa tattaacgct tacaatttag gtggcacttt tcggggaaat gtgcgcggaa
     9001 cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac
     9061 cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg
     9121 tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc
     9181 tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg
     9241 atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga
     9301 gcacttttaa agttctgcta tgtggcgcgg tattatcccg tattgacgcc gggcaagagc
     9361 aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag
     9421 aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga
     9481 gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg
     9541 cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga
     9601 atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgtagcaatg gcaacaacgt
     9661 tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact
     9721 ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt
     9781 ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg
     9841 ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta
     9901 tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac
     9961 tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta
    10021 aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt
    10081 tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttc
//
