LOCUS       sequence-45569-e       11697 bp    DNA     circular SYN 14-APR-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11697)
  AUTHORS   Miyamichi K, Amat F, Moussavi F, Wang C, Wickersham I, Wall NR, 
            Taniguchi H, Tasic B, Huang ZJ, He Z, Callaway EM, Horowitz MA, Luo 
            L
  TITLE     Cortical representations of olfactory input by trans-synaptic 
            tracing.
  JOURNAL   Nature. 2010 Dec 22. ():.
  PUBMED    21179085
REFERENCE   2  (bases 1 to 11697)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 14, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nature. 
            2010 Dec 22. ():."
COMMENT     Sequence Label: pBOB-synP-HTB
FEATURES             Location/Qualifiers
     source          1..11697
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(44..63)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        235..614
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        616..814
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     769..789
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     LTR             832..1012
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    1056..1181
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1674..1907
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             2092..2136
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2388..2505
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        2539..2986
                     /label=hSyn promoter
                     /note="human synapsin I promoter; confers neuron-specific 
                     expression (Kugler et al., 2003)"
     CDS             3077..3454
                     /codon_start=1
                     /gene="HIST1H2BJ"
                     /product="human histone H2B"
                     /label=H2B
                     /translation="MPEPAKSAPAPKKGSKKAVTKAQKKGGKKRKRSRKESYSIYVYKV
                     LKQVHPDTGISSKAMGIMNSFVNDIFERIAGEASRLAHYNKRSTITSREIQTAVRLLLP
                     GELAKHAVSEGTKAITKYTSAK"
     regulatory      3467..3476
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(3473..3475,3476..3478,3479..4189)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(3473..3475,3476..3478,3479..4189)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(3518..3539)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(3779..3798)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     4126..4147
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     CDS             4199..4264
                     /codon_start=1
                     /product="2A peptide from foot-and-mouth disease virus 
                     polyprotein"
                     /label=F2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="VKQTLNFDLLKLAGDVESNPGP"
     CDS             4745..4798
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     misc_feature    6503..7091
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(6556..6576)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(6974..6985)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             7498..7678
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(7704..7721)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    7710..7934
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      7980..8408
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(8067..8086)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     8277..8298
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(8417..8437)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        8422..8752
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      8603..8738
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     8665..8684
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        8800..8847
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             8866..9240
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    9370..9491
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(9407..9426)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     9461..9480
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(9540..9556)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(9540..9556)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(9553..9575)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    9564..9580
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(9588..9594,9595..9612,9613..9618))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    9633..9654
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(9771..9788)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(9942..10530)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(10022..10041)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(10701..11492,11493..11561))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     11324..11343
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(11562..11666)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gacggatcgg gagatctccc gatcccctat ggtcgactct cagtacaatc tgctctgatg
       61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg
      121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc
      181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt
      241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata
      301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc
      361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc
      421 attgacgtca atgggtggac tatttacggt aaactgccca cttggcagta catcaagtgt
      481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt
      541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca
      601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg
      661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc
      721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg
      781 gtaggcgtgt acggtgggag gtctatataa gcagcgcgtt ttgcctgtac tgggtctctc
      841 tggttagacc agatctgagc ctgggagctc tctggctaac tagggaaccc actgcttaag
      901 cctcaataaa gcttgccttg agtgcttcaa gtagtgtgtg cccgtctgtt gtgtgactct
      961 ggtaactaga gatccctcag acccttttag tcagtgtgga aaatctctag cagtggcgcc
     1021 cgaacaggga cctgaaagcg aaagggaaac cagagctctc tcgacgcagg actcggcttg
     1081 ctgaagcgcg cacggcaaga ggcgaggggc ggcgactggt gagtacgcca aaaattttga
     1141 ctagcggagg ctagaaggag agagatgggt gcgagagcgt cagtattaag cgggggagaa
     1201 ttagatcgcg atgggaaaaa attcggttaa ggccaggggg aaagaaaaaa tataaattaa
     1261 aacatatagt atgggcaagc agggagctag aacgattcgc agttaatcct ggcctgttag
     1321 aaacatcaga aggctgtaga caaatactgg gacagctaca accatccctt cagacaggat
     1381 cagaagaact tagatcatta tataatacag tagcaaccct ctattgtgtg catcaaagga
     1441 tagagataaa agacaccaag gaagctttag acaagataga ggaagagcaa aacaaaagta
     1501 agaccaccgc acagcaagcg gccgctgatc ttcagacttg gaggaggaga tatgagggac
     1561 aattggagaa gtgaattata taaatataaa gtagtaaaaa ttgaaccatt aggagtagca
     1621 cccaccaagg caaagagaag agtggtgcag agagaaaaaa gagcagtggg aataggagct
     1681 ttgttccttg ggttcttggg agcagcagga agcactatgg gcgcagcctc aatgacgctg
     1741 acggtacagg ccagacaatt attgtctggt atagtgcagc agcagaacaa tttgctgagg
     1801 gctattgagg cgcaacagca tctgttgcaa ctcacagtct ggggcatcaa gcagctccaa
     1861 gcaagaatcc tagctgtgga aagataccta aaggatcaac agctcctagg gatttggggt
     1921 tgctctggaa aactcatttg caccactgct gtgccttgga atgctagttg gagtaataaa
     1981 tctctggaac agatctggaa tcacacgacc tggatggagt gggacagaga aattaacaat
     2041 tacacaagct taatacactc cttaattgaa gaatcgcaaa accagcaaga aaagaatgaa
     2101 caagaattat tggaattaga taaatgggca agtttgtgga attggtttaa cataacaaat
     2161 tggctgtggt atataaaatt attcataatg atagtaggag gcttggtagg tttaagaata
     2221 gtttttgctg tactttctat agtgaataga gttaggcagg gatattcacc attatcgttt
     2281 cagacccacc tcccaatccc gaggggaccc gacaggcccg aaggaataga agaagaaggt
     2341 ggagagagag acagagacag atccattcga ttagtgaacg gatcaacttt taaaagaaaa
     2401 ggggggattg gggggtacag tgcaggggaa agaatagtag acataatagc aacagacata
     2461 caaactaaag aattacaaaa acaaattaca aaaattcaaa attttatcga tgctagactg
     2521 cagagggccc tgcgtatgag tgcaagtggg ttttaggacc aggatgaggc ggggtggggg
     2581 tgcctacctg acgaccgacc ccgacccact ggacaagcac ccaaccccca ttccccaaat
     2641 tgcgcatccc ctatcagaga gggggagggg aaacaggatg cggcgaggcg cgtgcgcact
     2701 gccagcttca gcaccgcgga cagtgccttc gcccccgcct ggcggcgcgc gccaccgccg
     2761 cctcagcact gaaggcgcgc tgacgtcact cgccggtccc ccgcaaactc cccttcccgg
     2821 ccaccttggt cgcgtccgcg ccgccgccgg cccagccgga ccgcaccacg cgaggcgcga
     2881 gatagggggg cacgggcgcg accatctgcg ctgcggcgcc ggcgactcag cgctgcctca
     2941 gtctgcggtg ggcagcggag gagtcgtgtc gtgcctgaga gcgcagtcga tctagacgat
     3001 aagggcgaat tctgcagata tccatcacac tggcggccgc tcgagcatgc atctagagga
     3061 tccgctagcc gccaccatgc cagagccagc gaagtctgct cccgccccga aaaagggctc
     3121 caagaaggcg gtgactaagg cgcagaagaa aggcggcaag aagcgcaagc gcagccgcaa
     3181 ggagagctat tccatctatg tgtacaaggt tctgaagcag gtccaccctg acaccggcat
     3241 ttcgtccaag gccatgggca tcatgaattc gtttgtgaac gacattttcg agcgcatcgc
     3301 aggtgaggct tcccgcctgg cgcattacaa caagcgctcg accatcacct ccagggagat
     3361 ccagacggcc gtgcgcctgc tgctgcctgg ggagttggcc aagcacgccg tgtccgaggg
     3421 tactaaggcc atcaccaagt acaccagcgc taaggatcca ccggtcgcca ccatggtgag
     3481 caagggcgag gagctgttca ccggggtggt gcccatcctg gtcgagctgg acggcgacgt
     3541 aaacggccac aagttcagcg tgtccggcga gggcgagggc gatgccacct acggcaagct
     3601 gaccctgaag ttcatctgca ccaccggcaa gctgcccgtg ccctggccca ccctcgtgac
     3661 caccctgacc tacggcgtgc agtgcttcag ccgctacccc gaccacatga agcagcacga
     3721 cttcttcaag tccgccatgc ccgaaggcta cgtccaggag cgcaccatct tcttcaagga
     3781 cgacggcaac tacaagaccc gcgccgaggt gaagttcgag ggcgacaccc tggtgaaccg
     3841 catcgagctg aagggcatcg acttcaagga ggacggcaac atcctggggc acaagctgga
     3901 gtacaactac aacagccaca acgtctatat catggccgac aagcagaaga acggcatcaa
     3961 ggtgaacttc aagatccgcc acaacatcga ggacggcagc gtgcagctcg ccgaccacta
     4021 ccagcagaac acccccatcg gcgacggccc cgtgctgctg cccgacaacc actacctgag
     4081 cacccagtcc gccctgagca aagaccccaa cgagaagcgc gatcacatgg tcctgctgga
     4141 gttcgtgacc gccgccggga tcactctcgg catggacgag ctgtacaagg gtagtggtgt
     4201 gaaacagact ttgaattttg accttctcaa gttggcggga gacgtggagt ccaacccagg
     4261 gcccatggcg cggctgctgc ccgcgctgct gctgctgctg ctgcccggta acgtgaccgg
     4321 taacgggtcc ggtaacggtt ctttgtcccg ttgccccccc ggtcagttcc gctgctcgga
     4381 gccgcccggt gcccacgggg agtgttaccc gcaggactgg ctgtgcgacg gacaccccga
     4441 ctgcgacgac gggcgggacg agtggggctg cgggaccagc gcgatccccg cggtgcccac
     4501 ggacaacggc acagaggctc ccactgcccc tgctcctgga cgtgctctgc cagccaggaa
     4561 tcacggccgc atgtggatgc tgatcactgc agtgctcctg tgctgcctgg tagctgtggg
     4621 tggtatcgct gcatggggga agtccaaagc aaaaagcagg tctgacatct tcagtcttga
     4681 aagcgcatcc aaggagctgc tggtgcctga caagagccag gcagacttgt tctccggtag
     4741 tggagagggc agaggaagtc tgctaacatg cggtgacgtc gaggagaatc ctggcccaat
     4801 ggttcctcag gctctcctgt ttgtacccct tctggttttt ccattgtgtt ttgggaaatt
     4861 ccctatttac acgataccag acaagcttgg tccctggagt ccgattgaca tacatcacct
     4921 cagctgccca aacaatttgg tagtggagga cgaaggatgc accaacctgt cagggttctc
     4981 ctacatggaa cttaaagttg gatacatctt agccataaaa gtgaacgggt tcacttgcac
     5041 aggcgttgtg acggaggctg aaacctacac taacttcgtt ggttatgtca caaccacgtt
     5101 caaaagaaag catttccgcc caacaccaga tgcatgtaga gccgcgtaca actggaagat
     5161 ggccggtgac cccagatatg aagagtctct acacaatccg taccctgact accgctggct
     5221 tcgaactgta aaaaccacca aggagtctct cgttatcata tctccaagtg tggcagattt
     5281 ggacccatat gacagatccc ttcactcgag ggtcttccct agcgggaagt gctcaggagt
     5341 agcggtgtct tctacctact gctccactaa ccacgattac accatttgga tgcccgagaa
     5401 tccgagacta gggatgtctt gtgacatttt taccaatagt agagggaaga gagcatccaa
     5461 agggagtgag acttgcggct ttgtagatga aagaggccta tataagtctt taaaaggagc
     5521 atgcaaactc aagttatgtg gagttctagg acttagactt atggatggaa catgggtctc
     5581 gatgcaaaca tcaaatgaaa ccaaatggtg ccctcccgat aagttggtga acctgcacga
     5641 ctttcgctca gacgaaattg agcaccttgt tgtagaggag ttggtcagga agagagagga
     5701 gtgtctggat gcactagagt ccatcatgac aaccaagtca gtgagtttca gacgtctcag
     5761 tcatttaaga aaacttgtcc ctgggtttgg aaaagcatat accatattca acaagacctt
     5821 gatggaagcc gatgctcact acaagtcagt cagaacttgg aatgagatcc tcccttcaaa
     5881 agggtgttta agagttgggg ggaggtgtca tcctcatgtg aacggggtgt ttttcaatgg
     5941 tataatatta ggacctgacg gcaatgtctt aatcccagag atgcaatcat ccctcctcca
     6001 gcaacatatg gagttgttgg aatcctcggt tatccccctt gtgcaccccc tggcagaccc
     6061 gtctaccgtt ttcaaggacg gtgacgaggc tgaggatttt gttgaagttc accttcccga
     6121 tgtgcacaat caggtctcag gagttgactt gggtctcccg aactggggga agtatgtatt
     6181 actgagtgca ggggccctga ctgccttgat gttgataatt ttcctgatga catgttgtag
     6241 aagagtcaat cgatcagaac ctacgcaaca caatctcaga gggacaggga gggaggtgtc
     6301 agtcactccc caaagcggga agatcatatc ttcatgggaa tcacacaaga gtgggggtga
     6361 gaccagactg taaggcgcgc cgtttaaacc tggaaaaaca tggagcaatc acaagtagca
     6421 acacagcagc taccaatgct gcttgtgcct ggctagaagc acaagaggag gaggaggtgg
     6481 gttttccagt cacacctcag acaatcaacc tctggattac aaaatttgtg aaagattgac
     6541 tggtattctt aactatgttg ctccttttac gctatgtgga tacgctgctt taatgccttt
     6601 gtatcatgct attgcttccc gtatggcttt cattttctcc tccttgtata aatcctggtt
     6661 gctgtctctt tatgaggagt tgtggcccgt tgtcaggcaa cgtggcgtgg tgtgcactgt
     6721 gtttgctgac gcaaccccca ctggttgggg cattgccacc acctgtcagc tcctttccgg
     6781 gactttcgct ttccccctcc ctattgccac ggcggaactc atcgccgcct gccttgcccg
     6841 ctgctggaca ggggctcggc tgttgggcac tgacaattcc gtggtgttgt cggggaagct
     6901 gacgtccttt ccatggctgc tcgcctgtgt tgccacctgg attctgcgcg ggacgtcctt
     6961 ctgctacgtc ccttcggccc tcaatccagc ggaccttcct tcccgcggcc tgctgccggc
     7021 tctgcggcct cttccgcgtc ttcgccttcg ccctcagacg agtcggatct ccctttgggc
     7081 cgcctccccg cctggaattc gagctcggta cctttaagac caatgactta caaggcagct
     7141 gtagatctta gccacttttt aaaagaaaag gggggactgg aagggctaat tcactcccaa
     7201 agaagacaag atatccttga tctgtggatc taccacacac aaggctactt ccctgattga
     7261 cagaactaca caccagggcc aggggtcaga tatccactga cctttggatg gtgctacaag
     7321 ctagtaccag ttgagccaga taagatagaa gaggccaata aaggagagaa caccagcttg
     7381 ttacaccctg tgagcctgca tgggatggat gacccggaga gagaagtgtt agagtggagg
     7441 tttgacagcc gcctagcatt tcatcacgtg gcccgagagc tgcatccgga ctgtactggg
     7501 tctctctggt tagaccagat ctgagcctgg gagctctctg gctaactagg gaacccactg
     7561 cttaagcctc aataaagctt gccttgagtg cttcaagtag tgtgtgcccg tctgttgtgt
     7621 gactctggta actagagatc cctcagaccc ttttagtcag tgtggaaaat ctctagcagg
     7681 gccgctttaa acccgctgat cagcctcgac tgtgccttct agttgccagc catctgttgt
     7741 ttgcccctcc cccgtgcctt ccttgaccct ggaaggtgcc actcccactg tcctttccta
     7801 ataaaatgag gaaattgcat cgcattgtct gagtaggtgt cattctattc tggggggtgg
     7861 ggtggggcag gacagcaagg gggaggattg ggaagacaat agcaggcatg ctggggatgc
     7921 ggtgggctct atggcttctg aggcggaaag aaccagctgg ggctctaggg ggtatcccca
     7981 cgcgccctgt agcggcgcat taagcgcggc gggtgtggtg gttacgcgca gcgtgaccgc
     8041 tacacttgcc agcgccctag cgcccgctcc tttcgctttc ttcccttcct ttctcgccac
     8101 gttcgccggc tttccccgtc aagctctaaa tcggggcatc cctttagggt tccgatttag
     8161 tgctttacgg cacctcgacc ccaaaaaact tgattagggt gatggttcac gtagtgggcc
     8221 atcgccctga tagacggttt ttcgcccttt gacgttggag tccacgttct ttaatagtgg
     8281 actcttgttc caaactggaa caacactcaa ccctatctcg gtctattctt ttgatttata
     8341 agggattttg gggatttcgg cctattggtt aaaaaatgag ctgatttaac aaaaatttaa
     8401 cgcgaattaa ttctgtggaa tgtgtgtcag ttagggtgtg gaaagtcccc aggctcccca
     8461 ggcaggcaga agtatgcaaa gcatgcatct caattagtca gcaaccaggt gtggaaagtc
     8521 cccaggctcc ccagcaggca gaagtatgca aagcatgcat ctcaattagt cagcaaccat
     8581 agtcccgccc ctaactccgc ccatcccgcc cctaactccg cccagttccg cccattctcc
     8641 gccccatggc tgactaattt tttttattta tgcagaggcc gaggccgcct ctgcctctga
     8701 gctattccag aagtagtgag gaggcttttt tggaggccta ggcttttgca aaaagctccc
     8761 gggagcttgt atatccattt tcggatctga tcagcacgtg ttgacaatta atcatcggca
     8821 tagtatatcg gcatagtata atacgacaag gtgaggaact aaaccatggc caagttgacc
     8881 agtgccgttc cggtgctcac cgcgcgcgac gtcgccggag cggtcgagtt ctggaccgac
     8941 cggctcgggt tctcccggga cttcgtggag gacgacttcg ccggtgtggt ccgggacgac
     9001 gtgaccctgt tcatcagcgc ggtccaggac caggtggtgc cggacaacac cctggcctgg
     9061 gtgtgggtgc gcggcctgga cgagctgtac gccgagtggt cggaggtcgt gtccacgaac
     9121 ttccgggacg cctccgggcc ggccatgacc gagatcggcg agcagccgtg ggggcgggag
     9181 ttcgccctgc gcgacccggc cggcaactgc gtgcacttcg tggccgagga gcaggactga
     9241 cacgtgctac gagatttcga ttccaccgcc gccttctatg aaaggttggg cttcggaatc
     9301 gttttccggg acgccggctg gatgatcctc cagcgcgggg atctcatgct ggagttcttc
     9361 gcccacccca acttgtttat tgcagcttat aatggttaca aataaagcaa tagcatcaca
     9421 aatttcacaa ataaagcatt tttttcactg cattctagtt gtggtttgtc caaactcatc
     9481 aatgtatctt atcatgtctg tataccgtcg acctctagct agagcttggc gtaatcatgg
     9541 tcatagctgt ttcctgtgtg aaattgttat ccgctcacaa ttccacacaa catacgagcc
     9601 ggaagcataa agtgtaaagc ctggggtgcc taatgagtga gctaactcac attaattgcg
     9661 ttgcgctcac tgcccgcttt ccagtcggga aacctgtcgt gccagctgca ttaatgaatc
     9721 ggccaacgcg cggggagagg cggtttgcgt attgggcgct cttccgcttc ctcgctcact
     9781 gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat cagctcactc aaaggcggta
     9841 atacggttat ccacagaatc aggggataac gcaggaaaga acatgtgagc aaaaggccag
     9901 caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag gctccgcccc
     9961 cctgacgagc atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc gacaggacta
    10021 taaagatacc aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt tccgaccctg
    10081 ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct ttctcaatgc
    10141 tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac
    10201 gaaccccccg ttcagcccga ccgctgcgcc ttatccggta actatcgtct tgagtccaac
    10261 ccggtaagac acgacttatc gccactggca gcagccactg gtaacaggat tagcagagcg
    10321 aggtatgtag gcggtgctac agagttcttg aagtggtggc ctaactacgg ctacactaga
    10381 aggacagtat ttggtatctg cgctctgctg aagccagtta ccttcggaaa aagagttggt
    10441 agctcttgat ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag
    10501 cagattacgc gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc tacggggtct
    10561 gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg tcatgagatt atcaaaaagg
    10621 atcttcacct agatcctttt aaattaaaaa tgaagtttta aatcaatcta aagtatatat
    10681 gagtaaactt ggtctgacag ttaccaatgc ttaatcagtg aggcacctat ctcagcgatc
    10741 tgtctatttc gttcatccat agttgcctga ctccccgtcg tgtagataac tacgatacgg
    10801 gagggcttac catctggccc cagtgctgca atgataccgc gagacccacg ctcaccggct
    10861 ccagatttat cagcaataaa ccagccagcc ggaagggccg agcgcagaag tggtcctgca
    10921 actttatccg cctccatcca gtctattaat tgttgccggg aagctagagt aagtagttcg
    10981 ccagttaata gtttgcgcaa cgttgttgcc attgctacag gcatcgtggt gtcacgctcg
    11041 tcgtttggta tggcttcatt cagctccggt tcccaacgat caaggcgagt tacatgatcc
    11101 cccatgttgt gcaaaaaagc ggttagctcc ttcggtcctc cgatcgttgt cagaagtaag
    11161 ttggccgcag tgttatcact catggttatg gcagcactgc ataattctct tactgtcatg
    11221 ccatccgtaa gatgcttttc tgtgactggt gagtactcaa ccaagtcatt ctgagaatag
    11281 tgtatgcggc gaccgagttg ctcttgcccg gcgtcaatac gggataatac cgcgccacat
    11341 agcagaactt taaaagtgct catcattgga aaacgttctt cggggcgaaa actctcaagg
    11401 atcttaccgc tgttgagatc cagttcgatg taacccactc gtgcacccaa ctgatcttca
    11461 gcatctttta ctttcaccag cgtttctggg tgagcaaaaa caggaaggca aaatgccgca
    11521 aaaaagggaa taagggcgac acggaaatgt tgaatactca tactcttcct ttttcaatat
    11581 tattgaagca tttatcaggg ttattgtctc atgagcggat acatatttga atgtatttag
    11641 aaaaataaac aaataggggt tccgcgcaca tttccccgaa aagtgccacc tgacgtc
//
