LOCUS       sequence-456077-        2519 bp    DNA     circular SYN 15-APR-2025
DEFINITION  T7 promoter driving expression of ulaR.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2519)
  AUTHORS   Piorino F, Styczynski MP
  TITLE     Harnessing Escherichia coli's Native Machinery for Detection of 
            Vitamin C (Ascorbate) Deficiency.
  JOURNAL   ACS Synth Biol. 2022 Nov 18;11(11):3592-3600. doi: 
            10.1021/acssynbio.2c00335. Epub 2022 Oct 27.
  PUBMED    36300901
REFERENCE   2  (bases 1 to 2519)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1021/acssynbio.2c00335"; journalName: "ACS Synth Biol"; date: 
            "2022-11-18- 18"; volume: "11"; issue: "11"; pages: "3592-3600"
COMMENT     Sequence Label: pUlaR
FEATURES             Location/Qualifiers
     source          1..2519
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      17..605
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     506..525
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     633..652
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        633..651
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     RBS             683..705
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     primer_bind     complement(1520..1538)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      1534..1581
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     CDS             complement(1599..2414)
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSKPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     2322..2341
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     primer_bind     2406..2425
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
ORIGIN
        1 agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa
       61 aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc
      121 cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgttcttcta gtgtagccgt
      181 agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc
      241 tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac
      301 gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca
      361 gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta tgagaaagcg
      421 ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag
      481 gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt
      541 ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat
      601 ggaaaaacgc cagcaacgcg atcccgcgaa attaatacga ctcactatag ggagaccaca
      661 acggtttccc tctagaaata attttgttta actttaagaa ggagatatac atatgactga
      721 agcacaaaga catcaaatcc tcctggaaat gctcgcacaa ttgggctttg tgaccgttga
      781 gaaagtcgtt gagcgtctgg gaatttcgcc tgccactgcg cgacgcgata tcaataaact
      841 tgacgaaagc ggcaaactga aaaaagtgcg caatggcgca gaagctatta cccaacagcg
      901 cccgcgctgg acgccgatga atctgcatca ggcgcagaat cacgatgaaa aagtacgtat
      961 cgctaaagcg gcctcgcagc tggttaatcc gggcgaaagc gtagtcatca actgcggctc
     1021 caccgcgttt ctgcttgggc gggaaatgtg tggcaagcca gtgcaaatca tcactaatta
     1081 tctaccgctg gcaaattacc tgatcgatca agaacatgac agcgtgatca ttatgggcgg
     1141 acagtacaac aaaagtcagt ccatcacttt aagcccgcag ggcagcgaaa acagtctcta
     1201 tgccgggcac tggatgttta ccagcggaaa agggctgacc gcagaagggt tgtataaaac
     1261 cgatatgctg acagcaatgg cagagcagaa gatgctgagc gtggtaggga aactggtggt
     1321 actggttgat agcagtaaga ttggcgaacg cgcgggaatg ctttttagcc gtgccgatca
     1381 aatcgatatg cttatcaccg gcaaaaatgc taacccggaa atcctgcaac aactggaagc
     1441 gcaaggggtc agcattctgc gtgtttaagt cgaccggctg ctaacaaagc ccgaaaggaa
     1501 gctgagttgg ctgctgccac cgctgagcaa taactagcat aaccccttgg ggcctctaaa
     1561 cgggtcttga ggggtttttt gctgaaagcc aattctgatt agaaaaactc atcgagcatc
     1621 aaatgaaact gcaatttatt catatcagga ttatcaatac catatttttg aaaaagccgt
     1681 ttctgtaatg aaggagaaaa ctcaccgagg cagttccata ggatggcaag atcctggtat
     1741 cggtctgcga ttccgactcg tccaacatca atacaaccta ttaatttccc ctcgtcaaaa
     1801 ataaggttat caagtgagaa atcaccatga gtgacgactg aatccggtga gaatggcaaa
     1861 agcttatgca tttctttcca gacttgttca acaggccagc cattacgctc gtcatcaaaa
     1921 tcactcgcat caaccaaacc gttattcatt cgtgattgcg cctgagcgag acgaaatacg
     1981 cgatcgctgt taaaaggaca attacaaaca ggaatcgaat gcaaccggcg caggaacact
     2041 gccagcgcat caacaatatt ttcacctgaa tcaggatatt cttctaatac ctggaatgct
     2101 gttttcccgg ggatcgcagt ggtgagtaac catgcatcat caggagtacg gataaaatgc
     2161 ttgatggtcg gaagaggcat aaattccgtc agccagttta gtctgaccat ctcatctgta
     2221 acatcattgg caacgctacc tttgccatgt ttcagaaaca actctggcgc atcgggcttc
     2281 ccatacaatc gatagattgt cgcacctgat tgcccgacat tatcgcgagc ccatttatac
     2341 ccatataaat cagcatccat gttggaattt aatcgcggct tcgagcaaga cgtttcccgt
     2401 tgaatatggc tcataacacc ccttgtatta ctgtttatgt aagcagacag ttttattgtt
     2461 catgatgata tatttttatc ttgtgcaatg taacatcaga gattttgaga cacaacgtg
//
