LOCUS       sequence-456139-        8336 bp    DNA     circular SYN 15-APR-2025
DEFINITION  Expression of mouse EHMT1 with deletion of aa 1-31.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8336)
  AUTHORS   Biligiri KK, Sharma NR, Mohanty A, Sarkar DP, Vemula PK, Rampalli S.
  TITLE     A cytoplasmic form of EHMT1N methylates viral proteins to enable 
            inclusion body maturation and efficient viral replication
  JOURNAL   PLOS Biology 22(11): e3002871.
REFERENCE   2  (bases 1 to 8336)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLOS 
            Biology 22(11): e3002871."
COMMENT     Sequence Label: EGFP_EHMT1V09
FEATURES             Location/Qualifiers
     source          1..8336
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     regulatory      2048..2057
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             complement(join(2073..2783,2784..2786,2787..2789))
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             complement(join(2073..2783,2784..2786,2787..2789))
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(2115..2136)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     2464..2483
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     2723..2744
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     regulatory      2786..2795
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     promoter        complement(2834..3037)
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     complement(2863..2883)
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     enhancer        3038..3341
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     rep_origin      complement(3516..4104)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(3596..3615)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     polyA_signal    4433..4480
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     primer_bind     4505..4524
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     CDS             complement(4712..5506)
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     CDS             complement(4712..5506)
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(4823..4842)
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     5433..5452
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     promoter        complement(5541..5898)
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      5555..5690
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     enhancer        5707..5898
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     5855..5875
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        complement(5900..6004)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      6031..6486
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6118..6137)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     6328..6349
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     polyA_signal    6493..6614
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6504..6523)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     6558..6577
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
ORIGIN
        1 ttactttggt gctccatttt gaagttggga tcaattccat caaccagcat gagaagcacc
       61 ttctgcagct caccctgcct ggcagagaag tacagctgct ttgggtggaa gcgaagtttc
      121 ttgggttttt cagagtctag agcgattaga gcactttcca aggtttcctt tcctgggccc
      181 tgggaaaggc cagatgtcgg cctcacgagc ccagccggtc cggcagggtc gaagcactct
      241 ggtgcctggg gggctgtgct ctgcgatctt tcatcctctg gggctccagc aatgctgccc
      301 gtggtcgtgt cagccctgcc ttcagcagcc aggctcttct cctgtccagg ggctagggtc
      361 actgtggaag ttgtgtctgc ttttgctatg gtcacctctt tggccttgga agcttcttcc
      421 ccacaatggg gacagtagct ggcattgttg actcgagagg cacagtcctt atggaaacga
      481 tgagagatgc tgctctcagg ctggcattcc atgaagttac cttcgtgatc cacgctctca
      541 gtggccatgc actggttgtt ggccagggtg ctgatctcgc ggctcttggg ggtttccatt
      601 cggcagctgc agagaggcac ttcctggagg ccatccgtcc ccagcacatc cggaccactg
      661 gccagccctt cattttctgt ctgggaagac agaattcctc tgacacggag atccagggaa
      721 tccagggaaa cttccatata gccagcgctg tctcccagag ctgcctgctc catgcttcct
      781 ggagatgact tacaagcctc ggaaccaagc atgctgctcg gcttctttct acttctccgc
      841 ctccttttcc gagcaggttt gatccagggg ctgtcagtct tccctctcct cttgagaaat
      901 ttcttcttga tactggattc agaactcagg tcagactcgt ctccaccatc ttcatcctcc
      961 cctgtgtcgg gagactcctg ttcctcctct gaatctccat ccatctttgc agcccggtca
     1021 gtctcagaca tgctctcttt agaagtccta ctatcctcgg tgggaaatgc agccgcttgc
     1081 tcagctccat ggtcctcgtc atcctccagc tcatcggagt cttcatcttc tgagtccatc
     1141 tccaagctct cgccatttac atgcagggca ctatcatcta aggctttctc gcccttggcg
     1201 cccacagtgg aatgggttat gcttttaaac atctcaatca ccgtcctctg ttttaatact
     1261 ttggtctttt tcttgggaac taaactatag gttcccattc ttcgtttttt cttccgagat
     1321 actgcagctg ctgtctggga ctttgtggtg gccatgtagc actgattctg aggtagcgac
     1381 tggtggaggg ctgggaaggt tggtaaaagc tgctgtcgtc cacattcaga aatgtttctg
     1441 ttgatatcct cttttggttc cttatggtct tgaacttctc tatggtcttt gctggctgca
     1501 tgcaggccca agatggactt cggcatggtc ttgcgtgccc tgtgaaccct gacatcagtg
     1561 cccgaggctg taggctttct gtcctctgtg tctgcactcc cttccccagg cacagtgggt
     1621 gctgtggttg gtgtctgagg aagtgcactc agagtcctgc atttactggc tcctccagga
     1681 agagtttttg cagcatgacc aggaagcgag gaggttagaa tgttgggagt cctcaacggc
     1741 tgcccctgca gggctggttt atttaagaaa tatccattgc tgccaatgac agatgtctgc
     1801 atgaagtcgt cagctgtgac atggttttgc ttccccactt ctgtgtctct ttctgaaacc
     1861 ccattttctg ccacccgaga cactctgttg gtgccttcct gtgggctagc tcttgtgttc
     1921 tcctgagtgt gtttgggtgc atttagatgg ctgggatccc cactcttttc acaagaccca
     1981 tttgtttctc catctgcagc catgggagtc tctccttctt gtttctctgt ggaaccttca
     2041 tcagcagcca tggtggcaga tctgagtccg gacttgtaca gctcgtccat gccgagagtg
     2101 atcccggcgg cggtcacgaa ctccagcagg accatgtgat cgcgcttctc gttggggtct
     2161 ttgctcaggg cggactgggt gctcaggtag tggttgtcgg gcagcagcac ggggccgtcg
     2221 ccgatggggg tgttctgctg gtagtggtcg gcgagctgca cgctgccgtc ctcgatgttg
     2281 tggcggatct tgaagttcac cttgatgccg ttcttctgct tgtcggccat gatatagacg
     2341 ttgtggctgt tgtagttgta ctccagcttg tgccccagga tgttgccgtc ctccttgaag
     2401 tcgatgccct tcagctcgat gcggttcacc agggtgtcgc cctcgaactt cacctcggcg
     2461 cgggtcttgt agttgccgtc gtccttgaag aagatggtgc gctcctggac gtagccttcg
     2521 ggcatggcgg acttgaagaa gtcgtgctgc ttcatgtggt cggggtagcg gctgaagcac
     2581 tgcacgccgt aggtcagggt ggtcacgagg gtgggccagg gcacgggcag cttgccggtg
     2641 gtgcagatga acttcagggt cagcttgccg taggtggcat cgccctcgcc ctcgccggac
     2701 acgctgaact tgtggccgtt tacgtcgccg tccagctcga ccaggatggg caccaccccg
     2761 gtgaacagct cctcgccctt gctcaccatg gtggcgaccg gtagcgctag cggatctgac
     2821 ggttcactaa accagctctg cttatataga cctcccaccg tacacgccta ccgcccattt
     2881 gcgtcaatgg ggcggagttg ttacgacatt ttggaaagtc ccgttgattt tggtgccaaa
     2941 acaaactccc attgacgtca atggggtgga gacttggaaa tccccgtgag tcaaaccgct
     3001 atccacgccc attgatgtac tgccaaaacc gcatcaccat ggtaatagcg atgactaata
     3061 cgtagatgta ctgccaagta ggaaagtccc ataaggtcat gtactgggca taatgccagg
     3121 cgggccattt accgtcattg acgtcaatag ggggcgtact tggcatatga tacacttgat
     3181 gtactgccaa gtgggcagtt taccgtaaat actccaccca ttgacgtcaa tggaaagtcc
     3241 ctattggcgt tactatggga acatacgtca ttattgacgt caatgggcgg gggtcgttgg
     3301 gcggtcagcc aggcgggcca tttaccgtaa gttatgtaac gcggaactcc atatatgggc
     3361 tatgaactaa tgaccccgta attgattact attaataact aatgcatggc ggtaatacgg
     3421 ttatccacag aatcagggga taacgcagga aagaacatgt gagcaaaagg ccagcaaaag
     3481 gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg cccccctgac
     3541 gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg actataaaga
     3601 taccaggcgt ttccccctgg aagctccctc gtgcgctctc ctgttccgac cctgccgctt
     3661 accggatacc tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca tagctcacgc
     3721 tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc
     3781 cccgttcagc ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc caacccggta
     3841 agacacgact tatcgccact ggcagcagcc actggtaaca ggattagcag agcgaggtat
     3901 gtaggcggtg ctacagagtt cttgaagtgg tggcctaact acggctacac tagaagaaca
     3961 gtatttggta tctgcgctct gctgaagcca gttaccttcg gaaaaagagt tggtagctct
     4021 tgatccggca aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt
     4081 acgcgcagaa aaaaaggatc tcaagaagat cctttgatct tttctacggg gtctgacgct
     4141 cagtggaacg aaaactcacg ttaagggatt ttggtcatga gattatcaaa aaggatcttc
     4201 acctagatcc ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa
     4261 cctgaggcta tggcagggcc tgccgccccg acgttggctg cgagccctgg gccttcaccc
     4321 gaacttgggg ggtggggtgg ggaaaaggaa gaaacgcggg cgtattggcc ccaatggggt
     4381 ctcggtgggg tatcgacaga gtgccagccc tgggaccgaa ccccgcgttt atgaacaaac
     4441 gacccaacac cgtgcgtttt attctgtctt tttattgccg tcatagcgcg ggttccttcc
     4501 ggtattgtct ccttccgtgt ttcagttagc ctccccctag ggtgggcgaa gaactccagc
     4561 atgagatccc cgcgctggag gatcatccag ccggcgtccc ggaaaacgat tccgaagccc
     4621 aacctttcat agaaggcggc ggtggaatcg aaatctcgtg atggcaggtt gggcgtcgct
     4681 tggtcggtca tttcgaaccc cagagtcccg ctcagaagaa ctcgtcaaga aggcgataga
     4741 aggcgatgcg ctgcgaatcg ggagcggcga taccgtaaag cacgaggaag cggtcagccc
     4801 attcgccgcc aagctcttca gcaatatcac gggtagccaa cgctatgtcc tgatagcggt
     4861 ccgccacacc cagccggcca cagtcgatga atccagaaaa gcggccattt tccaccatga
     4921 tattcggcaa gcaggcatcg ccatgggtca cgacgagatc ctcgccgtcg ggcatgctcg
     4981 ccttgagcct ggcgaacagt tcggctggcg cgagcccctg atgctcttcg tccagatcat
     5041 cctgatcgac aagaccggct tccatccgag tacgtgctcg ctcgatgcga tgtttcgctt
     5101 ggtggtcgaa tgggcaggta gccggatcaa gcgtatgcag ccgccgcatt gcatcagcca
     5161 tgatggatac tttctcggca ggagcaaggt gagatgacag gagatcctgc cccggcactt
     5221 cgcccaatag cagccagtcc cttcccgctt cagtgacaac gtcgagcaca gctgcgcaag
     5281 gaacgcccgt cgtggccagc cacgatagcc gcgctgcctc gtcttgcagt tcattcaggg
     5341 caccggacag gtcggtcttg acaaaaagaa ccgggcgccc ctgcgctgac agccggaaca
     5401 cggcggcatc agagcagccg attgtctgtt gtgcccagtc atagccgaat agcctctcca
     5461 cccaagcggc cggagaacct gcgtgcaatc catcttgttc aatcatgcga aacgatcctc
     5521 atcctgtctc ttgatcgatc tttgcaaaag cctaggcctc caaaaaagcc tcctcactac
     5581 ttctggaata gctcagaggc cgaggcggcc tcggcctctg cataaataaa aaaaattagt
     5641 cagccatggg gcggagaatg ggcggaactg ggcggagtta ggggcgggat gggcggagtt
     5701 aggggcggga ctatggttgc tgactaattg agatgcatgc tttgcatact tctgcctgct
     5761 ggggagcctg gggactttcc acacctggtt gctgactaat tgagatgcat gctttgcata
     5821 cttctgcctg ctggggagcc tggggacttt ccacacccta actgacacac attccacagc
     5881 tggttctttc cgcctcagga ctcttccttt ttcaatatta ttgaagcatt tatcagggtt
     5941 attgtctcat gagcggatac atatttgaat gtatttagaa aaataaacaa ataggggttc
     6001 cgcgcacatt tccccgaaaa gtgccacctg acgcgccctg tagcggcgca ttaagcgcgg
     6061 cgggtgtggt ggttacgcgc agcgtgaccg ctacacttgc cagcgcccta gcgcccgctc
     6121 ctttcgcttt cttcccttcc tttctcgcca cgttcgccgg ctttccccgt caagctctaa
     6181 atcgggggct ccctttaggg ttccgattta gtgctttacg gcacctcgac cccaaaaaac
     6241 ttgattaggg tgatggttca cgtagtgggc catcgccctg atagacggtt tttcgccctt
     6301 tgacgttgga gtccacgttc tttaatagtg gactcttgtt ccaaactgga acaacactca
     6361 accctatctc ggtctattct tttgatttat aagggatttt gccgatttcg gcctattggt
     6421 taaaaaatga gctgatttaa caaaaattta acgcgaattt taacaaaata ttaacgctta
     6481 caatttacgc gttaagatac attgatgagt ttggacaaac cacaactaga atgcagtgaa
     6541 aaaaatgctt tatttgtgaa atttgtgatg ctattgcttt atttgtaacc attataagct
     6601 gcaataaaca agttaacaac aacaattgca ttcattttat gtttcaggtt cagggggagg
     6661 tgtgggaggt tttttaaagc aagtaaaacc tctacaaatg tggtatggct gattatgatc
     6721 agttatctag atccggtgga tcccgggccc gcggtacctc tagggggtcg gcagcggctg
     6781 cagagctggt atctggaagg ccattctcct gaggctcctg ggctgcactg gcttgcctct
     6841 gggccagggc tgcgcttgag tgccgacact tggaagaccc acaccggcaa ctgaagagct
     6901 tgcccttgac gtcccaaaag cgctccccgt agtcgaaccc gagctgctcc ccagcctgaa
     6961 tcaggcgggt actgaagaag gcaatcctgg gaaaccgcag gtcctggtgt gacatgaaca
     7021 ctcgcacagg cacaaggttg ggttcgcagt ggtggtttat gaaccggctg acattcccat
     7081 agaaccgagc gtcaatgcag tatacctctc catccttatt gtcaagatca aagaggtaag
     7141 agtcctcttc ccgaacatca gcttcagagt ccgaaatcag ctcccctacg tattcgcaga
     7201 caaaggtgcc cagtgggata tcctggaggg accgcacacc ccagcccatg tcctgtgtcc
     7261 gataaagctg cagccttgcc ctgagaccat tttgcaccac acgattgcgg cagttcctcc
     7321 agcatgagca ggcatgattg cactcgaaga tcaagggtgg ttctgccatg ttaaactcag
     7381 gcagaagtcg gccatcctta tcataccagc agcgcatgct cagctggcca cacatgcagg
     7441 tgctagagga gcagtcatct acacacacgc agtactgcaa atgagtgatg ttcctgtcaa
     7501 tgttcatggg ggatgtcaca cagttctggg agacatactt atagttggta ggacacagct
     7561 cactgtccac agcattgaca cagggaatgg gaatgcgctc ataccctcga gcgatatccc
     7621 tgctcaccgt cttctcaaca gcaacgggct tgtcaggggc tgagtcccga agtgctttgc
     7681 tcatctgcaa tgcactccac acctgcgaac tgagacttgc acactgcaag ggagtctctc
     7741 cttccttgtt tttcagagta acatctgaat cccgagaaag aaagaggaca acacagtcgt
     7801 agcgattctc cctggctgcg atgtgcaggg gtgagtctcc atggatattc acagcatgca
     7861 ggtcacactt ggcagccaga agtatttcag ctatgtccac acagcctgaa aatgctgccc
     7921 agtgcagaca aatgttctcc tcgttgtccc ggatgttgat gtcagagccc ttagacagca
     7981 gcagcttcac cagctccacg tgcttgtact cagtggccca gatcataggt gtccatccac
     8041 cgtcatcctg gcagttgaca tccatctgtc catttgaaag cagatactga accacatcat
     8101 agtggccttt cttggcagcc aaatgcaaac atgtggagcc ctctgcgtcc ttcggatcca
     8161 cctgtgctcc agccttgatg aggtacttca ctgcatccaa gtggttgttc tctgcagcct
     8221 ccatcagtgg ggtccgctgg tcctctgagc aagtgtcaat attggcaccc gcctgaacca
     8281 gcatgtggca gatgtccacg tggccagcct ccgcagcagc atgtaatggg gaacgc
//
