LOCUS       sequence-456559-        9703 bp    DNA     circular SYN 14-APR-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9703)
  AUTHORS   Boehm JS, Zhao JJ, Yao J, Kim SY, Firestein R, Dunn IF, Sjostrom SK,
            Garraway LA, Weremowicz S, Richardson AL, Greulich H, Stewart CJ, 
            Mulvey LA, Shen RR, Ambrogio L, Hirozane-Kishikawa T, Hill DE, Vidal
            M, Meyerson M, Grenier JK, Hinkle G, Root DE, Roberts TM, Lander ES,
            Polyak K, Hahn WC
  TITLE     Integrative Genomic Approaches Identify IKBKE as a Breast Cancer 
            Oncogene (Kinase Library Clones)
  JOURNAL   Cell. 2007 Jun 15. 129(6):1065-1079.
  PUBMED    17574021
REFERENCE   2  (bases 1 to 9703)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 14, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell. 2007 
            Jun 15. 129(6):1065-1079."
COMMENT     Sequence Label: pWZL Neo Myr Flag MAP3K7
FEATURES             Location/Qualifiers
     source          1..9703
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        149..528
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        529..732
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     683..703
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     misc_feature    953..1152
                     /label=MMLV Psi
                     /note="packaging signal of Moloney murine leukemia virus 
                     (MMLV)"
     CDS             1353..1769
                     /label=gag (truncated)
                     /note="truncated Moloney murine leukemia virus (MMLV) gag 
                     gene lacking the start codon"
     primer_bind     1699..1721
                     /label=pLXSN 5'
                     /note="Murine stem cell virus, forward primer. Also called 
                     MSCV"
     primer_bind     1737..1753
                     /label=pBABE 5'
                     /note="Psi packaging signal, forward primer for pBABE 
                     vectors"
     regulatory      1787..1796
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             1793..1834
                     /codon_start=1
                     /product="N-myristoylation signal from Src kinase (Pellman 
                     et al., 1985; Kaplan et al., 1988)"
                     /label=myr
                     /translation="MGSSKSKPKDPSQR"
     CDS             1859..1882
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     protein_bind    1889..1913
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             complement(2731..2742)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     protein_bind    complement(3651..3675)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     misc_feature    3734..4195
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(3790..3807)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     4017..4036
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             4200..4994
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(4254..4273)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     4864..4883
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     LTR             5181..5650
                     /note="long terminal repeat from Moloney murine leukemia 
                     virus"
     promoter        5686..5790
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(5791..5859,5860..6651)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(6009..6028)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      8417..9005
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8906..8925
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     9159..9176
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(join(9280..9703,1..4))
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(9390..9411)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     9602..9621
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
ORIGIN
        1 cgcgccgatc ttcaatattg gccattagcc atattattca ttggttatat agcataaatc
       61 aatattggct attggccatt gcatacgttg tatctatatc ataatatgta catttatatt
      121 ggctcatgtc caatatgacc gccatgttgg cattgattat tgactagtta ttaatagtaa
      181 tcaattacgg ggtcattagt tcatagccca tatatggagt tccgcgttac ataacttacg
      241 gtaaatggcc cgcctggctg accgcccaac gacccccgcc cattgacgtc aataatgacg
      301 tatgttccca tagtaacgcc aatagggact ttccattgac gtcaatgggt ggagtattta
      361 cggtaaactg cccacttggc agtacatcaa gtgtatcata tgccaagtcc gccccctatt
      421 gacgtcaatg acggtaaatg gcccgcctgg cattatgccc agtacatgac cttacgggac
      481 tttcctactt ggcagtacat ctacgtatta gtcatcgcta ttaccatggt gatgcggttt
      541 tggcagtaca ccaatgggcg tggatagcgg tttgactcac ggggatttcc aagtctccac
      601 cccattgacg tcaatgggag tttgttttgg caccaaaatc aacgggactt tccaaaatgt
      661 cgtaataacc ccgccccgtt gacgcaaatg ggcggtaggc gtgtacggtg ggaggtctat
      721 ataagcagag ctggtttagt gaaccgcgcc agtcctccga ttgactgagt cgcccgggta
      781 cccgtgtatc caataaaccc tcttgcagtt gcatccgact tgtggtctcg ctgttccttg
      841 ggagggtctc ctctgagtga ttgactaccc gtcagcgggg gtctttcatt tgggggctcg
      901 tccgggatcg ggagacccct gcccagggac caccgaccca ccaccgggag gcaagctggc
      961 cagcaactta tctgtgtctg tccgattgtc tagtgtctat gactgatttt atgcgcctgc
     1021 gtcggtacta gttagctaac tagctctgta tctggcggac ccgtggtgga actgacgagt
     1081 tctgaacacc cggccgcaac cctgggagac gtcccaggga ctttgggggc cgtttttgtg
     1141 gcccgacctg aggaagggag tcgatgtgga atccgacccc gtcaggatat gtggttctgg
     1201 taggagacga gaacctaaaa cagttcccgc ctccgtctga atttttgctt tcggtttgga
     1261 accgaagccg cgcgtcttgt ctgctgcagc gctgcagcat cgttctgtgt tgtctctgtc
     1321 tgactgtgtt tctgtatttg tctgaaaatt agggccagac tgttaccact cccttaagtt
     1381 tgaccttagg tcactggaaa gatgtcgagc ggatcgctca caaccagtcg gtagatgtca
     1441 agaagagacg ttgggttacc ttctgctctg cagaatggcc aacctttaac gtcggatggc
     1501 cgcgagacgg cacctttaac cgagacctca tcacccaggt taagatcaag gtcttttcac
     1561 ctggcccgca tggacaccca gaccaggtcc cctacatcgt gacctgggaa gccttggctt
     1621 ttgacccccc tccctgggtc aagccctttg tacaccctaa gcctccgcct cctcttcctc
     1681 catccgcccc gtctctcccc cttgaacctc ctcgttcgac cccgcctcga tcctcccttt
     1741 atccagccct cactccttct ctaggcgccg gccggatcca gaattcgcca ccatggggtc
     1801 ttcaaaatct aaaccaaagg accccagcca gcgccggcgc aggatccgag gttaccttga
     1861 ctacaaagac gatgacgaca agcaattgac aagtttgtac aaaaaagttg gcatgtctac
     1921 agcctctgcc gcctcctcct cctcctcgtc ttcggccggt gagatgatcg aagccccttc
     1981 ccaggtcctc aactttgaag agatcgacta caaggagatc gaggtggaag aggttgttgg
     2041 aagaggagcc tttggagttg tttgcaaagc taagtggaga gcaaaagatg ttgctattaa
     2101 acaaatagaa agtgaatctg agaggaaagc gtttattgta gagcttcggc agttatcccg
     2161 tgtgaaccat cctaatattg taaagcttta tggagcctgc ttgaatccag tgtgtcttgt
     2221 gatggaatat gctgaagggg gctctttata taatgtgctg catggtgctg aaccattgcc
     2281 atattatact gctgcccacg caatgagttg gtgtttacag tgttcccaag gagtggctta
     2341 tcttcacagc atgcaaccca aagcgctaat tcacagggac ctgaaaccac caaacttact
     2401 gctggttgca ggggggacag ttctaaaaat ttgtgatttt ggtacagcct gtgacattca
     2461 gacacacatg accaataaca aggggagtgc tgcttggatg gcacctgaag tttttgaagg
     2521 tagtaattac agtgaaaaat gtgacgtctt cagctggggt attattcttt gggaagtgat
     2581 aacgcgtcgg aaaccctttg atgagattgg tggcccagct ttccgaatca tgtgggctgt
     2641 tcataatggt actcgaccac cactgataaa aaatttacct aagcccattg agagcctgat
     2701 gactcgttgt tggtctaaag atccttccca gcgcccttca atggaggaaa ttgtgaaaat
     2761 aatgactcac ttgatgcggt actttccagg agcagatgag ccattacagt atccttgtca
     2821 gtattcagat gaaggacaga gcaactctgc caccagtaca ggctcattca tggacattgc
     2881 ttctacaaat acgagtaaca aaagtgacac taatatggag caagttcctg ccacaaatga
     2941 tactattaag cgcttagaat caaaattgtt gaaaaatcat gcaaagcaac agagtgaatc
     3001 tggacgttta agcttgggag cctcccgtgg gagcagtgtg gagagcttgc ccccaacctc
     3061 tgagggcaag aggatgagtg ctgacatgtc tgaaatagaa gctaggatcg ccgcaaccac
     3121 aggcaacgga cagccaagac gtagatccat ccaagacttg actgtaactg gaacagaacc
     3181 tggtcaggtg agcagtaggt catccagtcc cagtgtcaga atgattacta cctcaggacc
     3241 aacctcagaa aagccaactc gaagtcatcc atggacccct gatgattcca cagataccaa
     3301 tggatcagat aactccatcc caatggctta tcttacactg gatcaccaac tacagcctct
     3361 agcaccgtgc ccaaactcca aagaatctat ggcagtgttt gaacagcatt gtaaaatggc
     3421 acaagaatat atgaaagttc aaacagaaat tgcattgtta ttacagagaa agcaagaact
     3481 agttgcagaa ctggaccagg atgaaaagga ccagcaaaat acatctcgcc tggtacagga
     3541 acataaaaag cttttagatg aaaacaaaag cctttctact tactaccagc aatgcaaaaa
     3601 acaactagag gtcatcagaa gtcagcagca gaaacgacaa ggcacttcat gcccaacttt
     3661 cttgtacaaa gtggtttgat ctagtcgacc aattccggtt attttccacc atattgccgt
     3721 cttttggcaa tgtgagggcc cggaaacctg gccctgtctt cttgacgagc attcctaggg
     3781 gtctttcccc tctcgccaaa ggaatgcaag gtctgttgaa tgtcgtgaag gaagcagttc
     3841 ctctggaagc ttcttgaaga caaacaacgt ctgtagcgac cctttgcagg cagcggaacc
     3901 ccccacctgg cgacaggtgc ctctgcggcc aaaagccacg tgtataagat acacctgcaa
     3961 aggcggcaca accccagtgc cacgttgtga gttggatagt tgtggaaaga gtcaaatggc
     4021 tctcctcaag cgtattcaac aaggggctga aggatgccca gaaggtaccc cattgtatgg
     4081 gatctgatct ggggcctcgg tgcacatgct ttacatgtgt ttagtcgagg ttaaaaaacg
     4141 tctaggcccc ccgaaccacg gggacgtggt tttcctttga aaaacacgat gataatacca
     4201 tgattgaaca agatggattg cacgcaggtt ctccggccgc ttgggtggag aggctattcg
     4261 gctatgactg ggcacaacag acaatcggct gctctgatgc cgccgtgttc cggctgtcag
     4321 cgcaggggcg cccggttctt tttgtcaaga ccgacctgtc cggtgccctg aatgaactgc
     4381 aggacgaggc agcgcggcta tcgtggctgg ccacgacggg cgttccttgc gcagctgtgc
     4441 tcgacgttgt cactgaagcg ggaagggact ggctgctatt gggcgaagtg ccggggcagg
     4501 atctcctgtc atctcacctt gctcctgccg agaaagtatc catcatggct gatgcaatgc
     4561 ggcggctgca tacgcttgat ccggctacct gcccattcga ccaccaagcg aaacatcgca
     4621 tcgagcgagc acgtactcgg atggaagccg gtcttgtcga tcaggatgat ctggacgaag
     4681 agcatcaggg gctcgcgcca gccgaactgt tcgccaggct caaggcgcgc atgcccgacg
     4741 gcgaggatct cgtcgtgacc catggcgatg cctgcttgcc gaatatcatg gtggaaaatg
     4801 gccgcttttc tggattcatc gactgtggcc ggctgggtgt ggcggaccgc tatcaggaca
     4861 tagcgttggc tacccgtgat attgctgaag agcttggcgg cgaatgggct gaccgcttcc
     4921 tcgtgcttta cggtatcgcc gctcccgatt cgcagcgcat cgccttctat cgccttcttg
     4981 acgagttctt ctgagcggga ctctggggtt cgcatcgata aaataaaaga ttttatttag
     5041 tctccagaaa aaggggggaa tgaaagaccc cacctgtagg tttggcaagc tagcttaagt
     5101 aacgccattt tgcaaggcat ggaaaaatac ataactgaga atagagaagt tcagatcaag
     5161 gtcaggaaca gatggaacag ctgaatatgg gccaaacagg atatctgtgg taagcagttc
     5221 ctgccccggc tcagggccaa gaacagatgg aacagctgaa tatgggccaa acaggatatc
     5281 tgtggtaagc agttcctgcc ccggctcagg gccaagaaca gatggtcccc agatgcggtc
     5341 cagccctcag cagtttctag agaaccatca gatgtttcca gggtgcccca aggacctgaa
     5401 atgaccctgt gccttatttg aactaaccaa tcagttcgct tctcgcttct gttcgcgcgc
     5461 ttctgctccc cgagctcaat aaaagagccc acaacccctc actcggggcg ccagtcctcc
     5521 gattgactga gtcgcccggg tacccgtgta tccaataaac cctcttgcag ttgcatccga
     5581 cttgtggtct cgctgttcct tgggagggtc tcctctgagt gattgactac ccgtcagcgg
     5641 gggtctttca caggccagtg gggccgcact tttcggggaa atgtgcgcgg aacccctatt
     5701 tgtttatttt tctaaataca ttcaaatatg tatccgctca tgagacaata accctgataa
     5761 atgcttcaat aatattgaaa aaggaagagt atgagtattc aacatttccg tgtcgccctt
     5821 attccctttt ttgcggcatt ttgccttcct gtttttgctc acccagaaac gctggtgaaa
     5881 gtaaaagatg ctgaagatca gttgggtgca cgagtgggtt acatcgaact ggatctcaac
     5941 agcggtaaga tccttgagag ttttcgcccc gaagaacgtt ttccaatgat gagcactttt
     6001 aaagttctgc tatgtggcgc ggtattatcc cgtattgacg ccgggcaaga gcaactcggt
     6061 cgccgcatac actattctca gaatgacttg gttgagtact caccagtcac agaaaagcat
     6121 cttacggatg gcatgacagt aagagaatta tgcagtgctg ccataaccat gagtgataac
     6181 actgcggcca acttacttct gacaacgatc ggaggaccga aggagctaac cgcttttttg
     6241 cacaacatgg gggatcatgt aactcgcctt gatcgttggg aaccggagct gaatgaagcc
     6301 ataccaaacg acgagcgtga caccacgatg cctgtagcaa tggcaacaac gttgcgcaaa
     6361 ctattaactg gcgaactact tactctagct tcccggcaac aattaataga ctggatggag
     6421 gcggataaag ttgcaggacc acttctgcgc tcggcccttc cggctggctg gtttattgct
     6481 gataaatctg gagccggtga gcgtgggtct cgcggtatca ttgcagcact ggggccagat
     6541 ggtaagccct cccgtatcgt agttatctac acgacgggga gtcaggcaac tatggatgaa
     6601 cgaaatagac agatcgctga gataggtgcc tcactgatta agcattggta actgtcagac
     6661 caagtttact catatatact ttagattgat ttgcggccgc gaaaaggaca agcagcgaaa
     6721 attcacgccc ccttgggagg tggcggcata tgcaaaggat agcactccca ctctactact
     6781 gggtatcata tgctgactgt atatgcatga ggatagcata tgctacccgg atacagatta
     6841 ggatagcata tactacccag atatagatta ggatagcata tgctacccag atatagatta
     6901 ggatagccta tgctacccag atataaatta ggatagcata tactacccag atatagatta
     6961 ggatagcata tgctacccag atatagatta ggatagccta tgctacccag atatagatta
     7021 ggatagcata tgctatccag atatttgggt agtatatgct acccatggca acattagccc
     7081 accgtgctct cagcgacctc gtgaatatga ggaccaacaa ccctgtgctt ggcgctcagg
     7141 cgcaagtgtg tgtaatttgt cctccagatc gcagcaatcg cgcccctatc ttggcccgcc
     7201 cacctactta tgcaggtatt ccccggggtg ccattagtgg ttttgtgggc aagtggtttg
     7261 accgcagtgg ttagcggggt tacaatcagc caagttatta cacccttatt ttacagtcca
     7321 aaaccgcagg gcggcgtgtg ggggctgacg cgtgccccca ctccacaatt tcaaaaaaaa
     7381 gagtggccac ttgtctttgt ttatgggccc cattggcgtg gagccccgtt taattttcgg
     7441 gggtgttaga gacaaccagt ggagtccgct gctgtcggcg tccactctct ttccccttgt
     7501 tacaaataga gtgtaacaac atggttcacc tgtcttggtc cctgcctggg acacatctta
     7561 ataaccccag tatcatattg cactaggatt atgtgttgcc catagccata aattcgtgtg
     7621 agatggacat ccagtcttta cggcttgtcc ccaccccatg gatttctatt gttaaagata
     7681 ttcagaatgt ttcattccta cactagtatt tattgcccaa ggggtttgtg agggttatat
     7741 tggtgtcata gcacaatgcc accactgaac cccccgtcca aattttattc tgggggcgtc
     7801 acctgaaacc ttgttttcga gcacctcaca tacaccttac tgttcacaac tcagcagtta
     7861 ttctattagc taaacgaagg agaatgaaga agcaggcgaa gattcaggag agttcactgc
     7921 ccgctccttg atcttcagcc actgcccttg tgactaaaat ggttcactac cctcgtggaa
     7981 tcctgacccc atgtaaataa aaccgtgaca gctcatgggg tgggagatat cgctgttcct
     8041 taggaccctt ttactaaccc taattcgata gcatatgctt cccgttgggt aacatatgct
     8101 attgaattag ggttagtctg gatagtatat actactaccc gggaagcata tgctacccgt
     8161 ttagggttaa caagggggcc ttataaacac tattgctaat gccctcttga gggtccgctt
     8221 atcggtagct acacaggccc ctctgattga cgttggtgta gcctcccgta gtcttcctgg
     8281 gcccctggga ggtacatgtc ggccgcaaaa ggatctaggt gaagatcctt tttgataatc
     8341 tcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac cccgtagaaa
     8401 agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa
     8461 aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc
     8521 cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgttcttcta gtgtagccgt
     8581 agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc
     8641 tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac
     8701 gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca
     8761 gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta tgagaaagcg
     8821 ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag
     8881 gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt
     8941 ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat
     9001 ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg ccttttgctc
     9061 acatgttctt tcctgcgtta tcccctgatt ctgtggataa ccgtattacc gcctttgagt
     9121 gagctgatac cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg agcgaggaag
     9181 cggaagagcg cccaatacgc aaaccgcctc tccccgcgcg ttggccgatt cattaatgca
     9241 actatggttg gcgcgttgta aacgttaata ttttgttaaa attcgcgtta aatttttgtt
     9301 aaatcagctc attttttaac caatagaccg aaatcggcaa aatcccttat aaatcaaaag
     9361 aatagcccga gatagagttg agtgttgttc cagtttggaa caagagtcca ctattaaaga
     9421 acgtggactc caacgtcaaa gggcgaaaaa ccgtctatca gggcgatggc ccactacgtg
     9481 aaccatcacc caaatcaagt tttttggggt cgaggtgccg taaagcacta aatcggaacc
     9541 ctaaagggag cccccgattt agagcttgac ggggaaagcc ggcgaacgtg gcgagaaagg
     9601 aagggaagaa agcgaaagga gcgggcgcta aggcgctggc aagtgtagcg gtcacgctgc
     9661 gcgtaaccac cacacccgcc gcgcttaatg cgccgctaca ggg
//
