LOCUS       sequence-456859-       12045 bp    DNA     circular SYN 15-APR-2025
DEFINITION  Fluorescent reporter with exogenous Rb.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12045)
  AUTHORS   Kim S, Armand J, Safonov A, Zhang M, Soni RK, Schwartz G, McGuinness
            JE, Hibshoosh H, Razavi P, Kim M, Chandarlapaty S, Yang HW
  TITLE     Sequential activation of E2F via Rb degradation and c-Myc drives 
            resistance to CDK4/6 inhibitors in breast cancer.
  JOURNAL   Cell Rep. 2023 Nov 28;42(11):113198. doi: 
            10.1016/j.celrep.2023.113198. Epub 2023 Oct 21.
  PUBMED    37865915
REFERENCE   2  (bases 1 to 12045)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell Rep.";
            date: "2023-11-28"; volume: "42(11):113198. doi"; pages: " 
            10.1016/j.celrep.2023.113198. Epub 2023 Oct 21"
COMMENT     Sequence Label: pLV-YFP-Rb-IRES-blasticidin
FEATURES             Location/Qualifiers
     source          1..12045
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    680..805
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1302..1535
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1720..1764
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2027..2142
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1 (lacking the first T)"
     promoter        2220..2431
                     /label=EF-1-alpha core promoter
                     /note="core promoter for human elongation factor 
                     EF-1-alpha"
     intron          2432..3370
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     3327..3347
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     CDS             3392..4102
                     /codon_start=1
                     /product="yellow fluorescent protein optimized for FRET 
                     with CyPet (Nguyen and Daugherty, 2005)"
                     /label=YPet
                     /translation="MSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     LLCTTGKLPVPWPTLVTTLGYGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYITADKQKNGIKA
                     NFKIRHNIEDGGVQLADHYQQNTPIGDGPVLLPDNHYLSYQSALFKDPNEKRDHMVLLE
                     FLTAAGITEGMNELY"
     misc_feature    7009..7558
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(7153..7170)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     7380..7399
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     misc_feature    8013..8601
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(8066..8086)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(8484..8495)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             8808..9441
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(9569..9585)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(9569..9585)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(9582..9604)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    9593..9609
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(9617..9623,9624..9641,9642..9647))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    9662..9683
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(9800..9817)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(9971..10559)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(10051..10070)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(10730..11521,11522..11590))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     11353..11372
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(11591..11695)
                     /gene="bla"
                     /label=AmpR promoter
     polyA_signal    11743..11877
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(11780..11799)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     11834..11853
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     LTR             join(12045,1..633)
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
ORIGIN
        1 ggaagggcta attcactccc aaagaagaca agatatcctt gatctgtgga tctaccacac
       61 acaaggctac ttccctgatt agcagaacta cacaccaggg ccaggggtca gatatccact
      121 gacctttgga tggtgctaca agctagtacc agttgagcca gataaggtag aagaggccaa
      181 taaaggagag aacaccagct tgttacaccc tgtgagcctg catgggatgg atgacccgga
      241 gagagaagtg ttagagtgga ggtttgacag ccgcctagca tttcatcacg tggcccgaga
      301 gctgcatccg gagtacttca agaactgctg atatcgagct tgctacaagg gactttccgc
      361 tggggacttt ccagggaggc gtggcctggg cgggactggg gagtggcgag ccctcagatc
      421 ctgcatataa gcagctgctt tttgcctgta ctgggtctct ctggttagac cagatctgag
      481 cctgggagct ctctggctaa ctagggaacc cactgcttaa gcctcaataa agcttgcctt
      541 gagtgcttca agtagtgtgt gcccgtctgt tgtgtgactc tggtaactag agatccctca
      601 gaccctttta gtcagtgtgg aaaatctcta gcagtggcgc ccgaacaggg acttgaaagc
      661 gaaagggaaa ccagaggagc tctctcgacg caggactcgg cttgctgaag cgcgcacggc
      721 aagaggcgag gggcggcgac tggtgagtac gccaaaaatt ttgactagcg gaggctagaa
      781 ggagagagat gggtgcgaga gcgtcagtat taagcggggg agaattagat cgcgatggga
      841 aaaaattcgg ttaaggccag ggggaaagaa aaaatataaa ttaaaacata tagtatgggc
      901 aagcagggag ctagaacgat tcgcagttaa tcctggcctg ttagaaacat cagaaggctg
      961 tagacaaata ctgggacagc tacaaccatc ccttcagaca ggatcagaag aacttagatc
     1021 attatataat acagtagcaa ccctctattg tgtgcatcaa aggatagaga taaaagacac
     1081 caaggaagct ttagacaaga tagaggaaga gcaaaacaaa agtaagacca ccgcacagca
     1141 agcggccggc cgctgatctt cagacctgga ggaggagata tgagggacaa ttggagaagt
     1201 gaattatata aatataaagt agtaaaaatt gaaccattag gagtagcacc caccaaggca
     1261 aagagaagag tggtgcagag agaaaaaaga gcagtgggaa taggagcttt gttccttggg
     1321 ttcttgggag cagcaggaag cactatgggc gcagcgtcaa tgacgctgac ggtacaggcc
     1381 agacaattat tgtctggtat agtgcagcag cagaacaatt tgctgagggc tattgaggcg
     1441 caacagcatc tgttgcaact cacagtctgg ggcatcaagc agctccaggc aagaatcctg
     1501 gctgtggaaa gatacctaaa ggatcaacag ctcctgggga tttggggttg ctctggaaaa
     1561 ctcatttgca ccactgctgt gccttggaat gctagttgga gtaataaatc tctggaacag
     1621 atttggaatc acacgacctg gatggagtgg gacagagaaa ttaacaatta cacaagctta
     1681 atacactcct taattgaaga atcgcaaaac cagcaagaaa agaatgaaca agaattattg
     1741 gaattagata aatgggcaag tttgtggaat tggtttaaca taacaaattg gctgtggtat
     1801 ataaaattat tcataatgat agtaggaggc ttggtaggtt taagaatagt ttttgctgta
     1861 ctttctatag tgaatagagt taggcaggga tattcaccat tatcgtttca gacccacctc
     1921 ccaaccccga ggggacccga caggcccgaa ggaatagaag aagaaggtgg agagagagac
     1981 agagacagat ccattcgatt agtgaacgga tctcgacggt atcgccttta aaagaaaagg
     2041 ggggattggg gggtacagtg caggggaaag aatagtagac ataatagcaa cagacataca
     2101 aactaaagaa ttacaaaaac aaattacaaa aattcaaaat tttcgggttt attacaggga
     2161 cagcagagat ccagtttatc gatgaggccc tttcgtcttc actcgaggtg cccgtcagtg
     2221 ggcagagcgc acatcgccca cagtccccga gaagttgggg ggaggggtcg gcaattgaac
     2281 cggtgcctag agaaggtggc gcggggtaaa ctgggaaagt gatgtcgtgt actggctccg
     2341 cctttttccc gagggtgggg gagaaccgta tataagtgca gtagtcgccg tgaacgttct
     2401 ttttcgcaac gggtttgccg ccagaacaca ggtaagtgcc gtgtgtggtt cccgcgggcc
     2461 tggcctcttt acgggttatg gcccttgcgt gccttgaatt acttccacct ggctgcagta
     2521 cgtgattctt gatcccgagc ttcgggttgg aagtgggtgg gagagttcga ggccttgcgc
     2581 ttaaggagcc ccttcgcctc gtgcttgagt tgaggcctgg cctgggcgct ggggccgccg
     2641 cgtgcgaatc tggtggcacc ttcgcgcctg tctcgctgct ttcgataagt ctctagccat
     2701 ttaaaatttt tgatgacctg ctgcgacgct ttttttctgg caagatagtc ttgtaaatgc
     2761 gggccaagat ctgcacactg gtatttcggt ttttggggcc gcgggcggcg acggggcccg
     2821 tgcgtcccag cgcacatgtt cggcgaggcg gggcctgcga gcgcggccac cgagaatcgg
     2881 acgggggtag tctcaagctg gccggcctgc tctggtgcct ggcctcgcgc cgccgtgtat
     2941 cgccccgccc tgggcggcaa ggctggcccg gtcggcacca gttgcgtgag cggaaagatg
     3001 gccgcttccc ggccctgctg cagggagctc aaaatggagg acgcggcgct cgggagagcg
     3061 ggcgggtgag tcacccacac aaaggaaaag ggcctttccg tcctcagccg tcgcttcatg
     3121 tgactccacg gagtaccggg cgccgtccag gcacctcgat tagttctcga gcttttggag
     3181 tacgtcgtct ttaggttggg gggaggggtt ttatgcgatg gagtttcccc acactgagtg
     3241 ggtggagact gaagttaggc cagcttggca cttgatgtaa ttctccttgg aatttgccct
     3301 ttttgagttt ggatcttggt tcattctcaa gcctcagaca gtggttcaaa gtttttttct
     3361 tccatttcag gtgtcgtgag gatccgccac catgtctaaa ggtgaagaat tattcactgg
     3421 tgttgtccca attttggttg aattagatgg tgatgttaat ggtcacaaat tttctgtctc
     3481 cggtgaaggt gaaggtgatg ctacgtacgg taaattgacc ttaaaattac tctgtactac
     3541 tggtaaattg ccagttccat ggccaacctt agtcactact ttaggttatg gtgttcaatg
     3601 ttttgctaga tacccagatc atatgaaaca acatgacttt ttcaagtctg ccatgccaga
     3661 aggttatgtt caagaaagaa ctattttttt caaagatgac ggtaactaca agaccagagc
     3721 tgaagtcaag tttgaaggtg ataccttagt taatagaatc gaattaaaag gtattgattt
     3781 taaagaagat ggtaacattt taggtcacaa attggaatac aactataact ctcacaatgt
     3841 ttacatcact gctgacaaac aaaagaatgg tatcaaagct aacttcaaaa ttagacacaa
     3901 cattgaagat ggtggtgttc aattagctga ccattatcaa caaaatactc caattggtga
     3961 tggtccagtc ttgttaccag acaaccatta cttatcctat caatctgcct tattcaaaga
     4021 tccaaacgaa aagagagacc acatggtctt gttagaattt ttgactgctg ctggtattac
     4081 cgagggtatg aatgaattgt acctcgagat gccgcccaaa accccccgaa aaacggccgc
     4141 caccgccgcc gctgccgccg cggaaccccc ggcaccgccg ccgccgcccc ctcctgagga
     4201 ggacccagag caggacagcg gcccggagga cctgcctctc gtcaggcttg agtttgaaga
     4261 aacagaagaa cctgatttta ctgcattatg tcagaaatta aagataccag atcatgtcag
     4321 agagagagct tggttaactt gggagaaagt ttcatctgtg gatgaagtat tgggaggtta
     4381 tattcaaaag aaaaaggaac tgtggggaat ctgtatcttt attgcagcag ttgacctaga
     4441 tgagatgtcg ttcactttta ctgagctaca gaaaaacata gaaatcagtg tccataaatt
     4501 ctttaactta ctaaaagaaa ttgataccag taccaaagtt gataatgcta tgtcaagact
     4561 gttgaagaag tatgatgtat tgtttgcact cttcagcaaa ttggaaagga catgtgaact
     4621 tatatatttg acacaaccca gcagttcgat atctactgaa ataaattctg cattggtgct
     4681 aaaagtttct tggatcacat ttttattagc taaaggggaa gtattacaaa tggaagatga
     4741 tctggtgatt tcatttcagt taatgctatg tgtccttgac tattttatta aactctcacc
     4801 tcccatgttg ctcaaagaac catataaaac agctgttata cccattaatg gttcacctcg
     4861 aacacccagg cgaggtcaga acaggagtgc acggatagca aaacaactag aaaatgatac
     4921 aagaattatt gaagttctct gtaaagaaca tgaatgtaat atagatgagg tgaaaaatgt
     4981 ttatttcaaa aattttatac cttttatgaa ttctcttgga cttgtaacat ctaatggact
     5041 tccagaggtt gaaaatcttt ctaaacgata cgaagaaatt tatcttaaaa ataaagatct
     5101 agatgcaaga ttatttttgg atcatgataa aactcttcag actgattcta tagacagttt
     5161 tgaaacacag agaacaccac gaaaaagtaa ccttgatgaa gaggtgaatg taattcctcc
     5221 acacactcca gttaggactg ttatgaacac tatccaacaa ttaatgatga ttttaaattc
     5281 agcaagtgat caaccttcag aaaatctgat ttcctatttt aacaactgca cagtgaatcc
     5341 aaaagaaagt atactgaaaa gagtgaagga tataggatac atctttaaag agaaatttgc
     5401 taaagctgtg ggacagggtt gtgtcgaaat tggatcacag cgatacaaac ttggagttcg
     5461 cttgtattac cgagtaatgg aatccatgct taaatcagaa gaagaacgat tatccattca
     5521 aaattttagc aaacttctga atgacaacat ttttcatatg tctttattgg cgtgcgctct
     5581 tgaggttgta atggccacat atagcagaag tacatctcag aatcttgatt ctggaacaga
     5641 tttgtctttc ccatggattc tgaatgtgct taatttaaaa gcctttgatt tttacaaagt
     5701 gatcgaaagt tttatcaaag cagaaggcaa cttgacaaga gaaatgataa aacatttaga
     5761 acgatgtgaa catcgaatca tggaatccct tgcatggctc tcagattcac ctttatttga
     5821 tcttattaaa caatcaaagg accgagaagg accaactgat caccttgaat ctgcttgtcc
     5881 tcttaatctt cctctccaga ataatcacac tgcagcagat atgtatcttt ctcctgtaag
     5941 atctccaaag aaaaaaggtt caactacgcg tgtaaattct actgcaaatg cagagacaca
     6001 agcaacctca gccttccaga cccagaagcc attgaaatct acctctcttt cactgtttta
     6061 taaaaaagtg tatcggctag cctatctccg gctaaataca ctttgtgaac gccttctgtc
     6121 tgagcaccca gaattagaac atatcatctg gacccttttc cagcacaccc tgcagaatga
     6181 gtatgaactc atgagagaca ggcatttgga ccaaattatg atgtgttcca tgtatggcat
     6241 atgcaaagtg aagaatatag accttaaatt caaaatcatt gtaacagcat acaaggatct
     6301 tcctcatgct gttcaggaga cattcaaacg tgttttgatc aaagaagagg agtatgattc
     6361 tattatagta ttctataact cggtcttcat gcagagactg aaaacaaata ttttgcagta
     6421 tgcttccacc aggcccccta ccttgtcacc aatacctcac attcctcgaa gcccttacaa
     6481 gtttcctagt tcacccttac ggattcctgg agggaacatc tatatttcac ccctgaagag
     6541 tccatataaa atttcagaag gtctgccaac accaacaaaa atgactccaa gatcaagaat
     6601 cttagtatca attggtgaat cattcgggac ttctgagaag ttccagaaaa taaatcagat
     6661 ggtatgtaac agcgaccgtg tgctcaaaag aagtgctgaa ggaagcaacc ctcctaaacc
     6721 actgaaaaaa ctacgctttg atattgaagg atcagatgaa gcagatggaa gtaaacatct
     6781 cccaggagag tccaaatttc agcagaaact ggcagaaatg acttctactc gaacacgaat
     6841 gcaaaagcag aaaatgaatg atagcatgga tacctcaaac aaggaagaga aataagaatt
     6901 cctcgagggc ggccgctcta gagtcgacgg gccgcggtaa caattgttaa ctaacttaag
     6961 ctagcaacgg tttccctcta gcgggatcaa ttccgccccc cccccctaac gttactggcc
     7021 gaagccgctt ggaataaggc cggtgtgcgt ttgtctatat gttattttcc accatattgc
     7081 cgtcttttgg caatgtgagg gcccggaaac ctggccctgt cttcttgacg agcattccta
     7141 ggggtctttc ccctctcgcc aaaggaatgc aaggtctgtt gaatgtcgtg aaggaagcag
     7201 ttcctctgga agcttcttga agacaaacaa cgtctgtagc gaccctttgc aggcagcgga
     7261 accccccacc tggcgacagg tgcctctgcg gccaaaagcc acgtgtataa gatacacctg
     7321 caaaggcggc acaaccccag tgccacgttg tgagttggat agttgtggaa agagtcaaat
     7381 ggctctcctc aagcgtattc aacaaggggc tgaaggatgc ccagaaggta ccccattgta
     7441 tgggatctga tctggggcct cggtgcacat gctttacatg tgtttagtcg aggttaaaaa
     7501 aacgtctagg ccccccgaac cacggggacg tggttttcct ttgaaaaaca cgataatacc
     7561 atggtcatga aaacatttaa catttctcaa caagatctag aattagtaga agtagcgaca
     7621 gagaagatta caatgcttta tgaggataat aaacatcatg tgggagcggc aattcgtacg
     7681 aaaacaggag aaatcatttc ggcagtacat attgaagcgt atataggacg agtaactgtt
     7741 tgtgcagaag ccattgcgat tggtagtgca gtttcgaatg gacaaaagga ttttgacacg
     7801 attgtagctg ttagacaccc ttattctgac gaagtagata gaagtattcg agtggtaagt
     7861 ccttgtggta tgtgtaggga gttgatttca gactatgcac cagattgttt tgtgttaata
     7921 gaaatgaatg gcaagttagt caaaactacg attgaagaac tcattccact caaatatacc
     7981 cgaaattaac ccgggcgggg cgcgtctgga acaatcaacc tctggattac aaaatttgtg
     8041 aaagattgac tggtattctt aactatgttg ctccttttac gctatgtgga tacgctgctt
     8101 taatgccttt gtatcatgct attgcttccc gtatggcttt cattttctcc tccttgtata
     8161 aatcctggtt gctgtctctt tatgaggagt tgtggcccgt tgtcaggcaa cgtggcgtgg
     8221 tgtgcactgt gtttgctgac gcaaccccca ctggttgggg cattgccacc acctgtcagc
     8281 tcctttccgg gactttcgct ttccccctcc ctattgccac ggcggaactc atcgccgcct
     8341 gccttgcccg ctgctggaca ggggctcggc tgttgggcac tgacaattcc gtggtgttgt
     8401 cggggaagct gacgtccttt ccatggctgc tcgcctgtgt tgccacctgg attctgcgcg
     8461 ggacgtcctt ctgctacgtc ccttcggccc tcaatccagc ggaccttcct tcccgcggcc
     8521 tgctgccggc tctgcggcct cttccgcgtc ttcgccttcg ccctcagacg agtcggatct
     8581 ccctttgggc cgcctccccg cctggaatta attctgcagt cgagacctag aaaaacatgg
     8641 agcaatcaca agtagcaata cagcagctac caatgctgat tgtgcctggc tagaagcaca
     8701 agaggaggag gaggtgggtt ttccagtcac acctcaggta cctttaagac caatgactta
     8761 caaggcagct gtagatctta gccacttttt aaaagaaaag aggggactgg aagggctaat
     8821 tcactcccaa cgaagacaag atatccttga tctgtggatc taccacacac aaggctactt
     8881 ccctgattag cagaactaca caccagggcc aggggtcaga tatccactga cctttggatg
     8941 gtgctacaag ctagtaccag ttgagccaga taaggtagaa gaggccaata aaggagagaa
     9001 caccagcttg ttacaccctg tgagcctgca tgggatggat gacccggaga gagaagtgtt
     9061 agagtggagg tttgacagcc gcctagcatt tcatcacgtg gcccgagagc tgcatccgga
     9121 gtacttcaag aactgctgat atcgagcttg ctacaaggga ctttccgctg gggactttcc
     9181 agggaggcgt ggcctgggcg ggactgggga gtggcgagcc ctcagatcct gcatataagc
     9241 agctgctttt tgcctgtact gggtctctct ggttagacca gatctgagcc tgggagctct
     9301 ctggctaact agggaaccca ctgcttaagc ctcaataaag cttgccttga gtgcttcaag
     9361 tagtgtgtgc ccgtctgttg tgtgactctg gtaactagag atccctcaga cccttttagt
     9421 cagtgtggaa aatctctagc agtagtagtt catgtcatct tattattcag tatttataac
     9481 ttgcaaagaa atgaatatca gagagtgaga ggccttgaca ttgctagcgt ttaccgtcga
     9541 cctctagcta gagcttggcg taatcatggt catagctgtt tcctgtgtga aattgttatc
     9601 cgctcacaat tccacacaac atacgagccg gaagcataaa gtgtaaagcc tggggtgcct
     9661 aatgagtgag ctaactcaca ttaattgcgt tgcgctcact gcccgctttc cagtcgggaa
     9721 acctgtcgtg ccagctgcat taatgaatcg gccaacgcgc ggggagaggc ggtttgcgta
     9781 ttgggcgctc ttccgcttcc tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc
     9841 gagcggtatc agctcactca aaggcggtaa tacggttatc cacagaatca ggggataacg
     9901 caggaaagaa catgtgagca aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt
     9961 tgctggcgtt tttccatagg ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa
    10021 gtcagaggtg gcgaaacccg acaggactat aaagatacca ggcgtttccc cctggaagct
    10081 ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc
    10141 cttcgggaag cgtggcgctt tctcatagct cacgctgtag gtatctcagt tcggtgtagg
    10201 tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct
    10261 tatccggtaa ctatcgtctt gagtccaacc cggtaagaca cgacttatcg ccactggcag
    10321 cagccactgg taacaggatt agcagagcga ggtatgtagg cggtgctaca gagttcttga
    10381 agtggtggcc taactacggc tacactagaa gaacagtatt tggtatctgc gctctgctga
    10441 agccagttac cttcggaaaa agagttggta gctcttgatc cggcaaacaa accaccgctg
    10501 gtagcggtgg tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag
    10561 aagatccttt gatcttttct acggggtctg acgctcagtg gaacgaaaac tcacgttaag
    10621 ggattttggt catgagatta tcaaaaagga tcttcaccta gatcctttta aattaaaaat
    10681 gaagttttaa atcaatctaa agtatatatg agtaaacttg gtctgacagt taccaatgct
    10741 taatcagtga ggcacctatc tcagcgatct gtctatttcg ttcatccata gttgcctgac
    10801 tccccgtcgt gtagataact acgatacggg agggcttacc atctggcccc agtgctgcaa
    10861 tgataccgcg agacccacgc tcaccggctc cagatttatc agcaataaac cagccagccg
    10921 gaagggccga gcgcagaagt ggtcctgcaa ctttatccgc ctccatccag tctattaatt
    10981 gttgccggga agctagagta agtagttcgc cagttaatag tttgcgcaac gttgttgcca
    11041 ttgctacagg catcgtggtg tcacgctcgt cgtttggtat ggcttcattc agctccggtt
    11101 cccaacgatc aaggcgagtt acatgatccc ccatgttgtg caaaaaagcg gttagctcct
    11161 tcggtcctcc gatcgttgtc agaagtaagt tggccgcagt gttatcactc atggttatgg
    11221 cagcactgca taattctctt actgtcatgc catccgtaag atgcttttct gtgactggtg
    11281 agtactcaac caagtcattc tgagaatagt gtatgcggcg accgagttgc tcttgcccgg
    11341 cgtcaatacg ggataatacc gcgccacata gcagaacttt aaaagtgctc atcattggaa
    11401 aacgttcttc ggggcgaaaa ctctcaagga tcttaccgct gttgagatcc agttcgatgt
    11461 aacccactcg tgcacccaac tgatcttcag catcttttac tttcaccagc gtttctgggt
    11521 gagcaaaaac aggaaggcaa aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt
    11581 gaatactcat actcttcctt tttcaatatt attgaagcat ttatcagggt tattgtctca
    11641 tgagcggata catatttgaa tgtatttaga aaaataaaca aataggggtt ccgcgcacat
    11701 ttccccgaaa agtgccacct gacgtcgacg gatcgggaga tcaacttgtt tattgcagct
    11761 tataatggtt acaaataaag caatagcatc acaaatttca caaataaagc atttttttca
    11821 ctgcattcta gttgtggttt gtccaaactc atcaatgtat cttatcatgt ctggatcaac
    11881 tggataactc aagctaacca aaatcatccc aaacttccca ccccataccc tattaccact
    11941 gccaattacc tgtggtttca tttactctaa acctgtgatt cctctgaatt attttcattt
    12001 taaagaaatt gtatttgtta aatatgtact acaaacttag tagtt
//
