LOCUS       sequence-456998-        8278 bp    DNA     circular SYN 15-APR-2025
DEFINITION  It presents the TRE3VG inducible promoter allowing the dox-dependent
            inducible activation of mCherry through the OPTi-OX platform. The 
            CLYBL GSH supports higher transgene expression.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8278)
  AUTHORS   Guichardaz M, Sveva B, Balmas E, Bertero A
  TITLE     Overcoming the Silencing of Doxycycline-Inducible Promoters in 
            hiPSC-derived Cardiomyocytes
  JOURNAL   Zenodo
REFERENCE   2  (bases 1 to 8278)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Zenodo"
COMMENT     Sequence Label: pCLYBL-Neo_TRE3VG_mCherry_Responder
FEATURES             Location/Qualifiers
     source          1..8278
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    127..160
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     polyA_signal    161..385
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     CDS             complement(420..1220)
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="GPRIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQG
                     RPVLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDL
                     LSSHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEE
                     HQGLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQD
                     IALATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(531..550)
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     1141..1160
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     CDS             complement(1215..1268)
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     misc_feature    1292..1317
                     /label=SA
                     /note="splice acceptor site"
     protein_bind    1318..1351
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     primer_bind     complement(2212..2228)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2212..2228)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(2225..2247)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    2236..2252
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(2260..2266,2267..2284,2285..2290))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    2305..2326
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(2443..2460)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(2614..3202)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(2694..2713)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(3380..4189)
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     4103..4122
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     primer_bind     4181..4200
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     primer_bind     complement(4331..4353)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     4453..4472
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     4666..4688
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     4680..4697
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     4681..4697
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     polyA_signal    6774..6855
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     6799..6818
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     CDS             complement(7052..7762)
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(7330..7349)
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     primer_bind     7599..7617
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     regulatory      7759..7768
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     promoter        complement(7775..8124)
                     /label=TRE3GV promoter
                     /note="3rd-generation Tet-responsive promoter that can be 
                     activated by binding of Tet-On(R) 3G, optimized for 
                     retroviral and lentiviral vectors"
     primer_bind     complement(7812..7836)
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     protein_bind    7882..7900
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    7918..7936
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    7954..7972
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    7990..8008
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    8026..8044
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    8062..8080
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    8098..8116
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
ORIGIN
        1 gctcggggat gcggggggag cgccggaccg gagcggagcc ccgggcggct cgctgctgcc
       61 ccctagcggg ggagggacgt aattacatcc ctgggggctt tggggggggg ctgtccccgt
      121 gagctcataa cttcgtataa tgtatgctat acgaagttat ccatagagcc caccgcatcc
      181 ccagcatgcc tgctattgtc ttcccaatcc tcccccttgc tgtcctgccc caccccaccc
      241 cccagaatag aatgacacct actcagacaa tgcgatgcaa tttcctcatt ttattaggaa
      301 aggacagtgg gagtggcacc ttccagggtc aaggaaggca cgggggaggg gcaaacaaca
      361 gatggctggc aactagaagg cacagtcagg caccgggctt gcgggtcatg caccaggtgt
      421 tagaagaact cgtcaagaag gcgatagaag gcgatgcgct gcgaatcggg agcggcgata
      481 ccgtaaagca cgaggaagcg gtcagcccat tcgccgccaa gctcttcagc aatatcacgg
      541 gtagccaacg ctatgtcctg atagcggtcc gccacaccca gccggccaca gtcgatgaat
      601 ccagaaaagc ggccattttc caccatgata ttcggcaagc aggcatcgcc atgggtcacg
      661 acgagatcct cgccgtcggg catgcgcgcc ttgagcctgg cgaacagttc ggctggcgcg
      721 agcccctgat gctcttcgtc cagatcatcc tgatcgacaa gaccggcttc catccgagta
      781 cgtgctcgct cgatgcgatg tttcgcttgg tggtcgaatg ggcaggtagc cggatcaagc
      841 gtatgcagcc gccgcattgc atcagccatg atggatactt tctcggcagg agcaaggtga
      901 gatgacagga gatcctgccc cggcacttcg cccaatagca gccagtccct tcccgcttca
      961 gtgacaacgt cgagcacagc tgcgcaagga acgcccgtcg tggccagcca cgatagccgc
     1021 gctgcctcgt cctgcagttc attcagggca ccggacaggt cggtcttgac aaaaagaacc
     1081 gggcgcccct gcgctgacag ccggaacacg gcggcatcag agcagccgat tgtctgttgt
     1141 gcccagtcat agccgaatag cctctccacc caagcggccg gagaacctgc gtgcaatcca
     1201 tcttgttcaa tcctagggcc gggattctcc tccacgtcac cgcatgttag aagacttcct
     1261 ctgccctctc cgctgccaga tctctcgagg ccctgtggga ggaagagaag aggtcagata
     1321 acttcgtata atgtatgcta tacgaagtta tgcggccgct gggagaaggg taagagagct
     1381 caatcctcat cttacataga aggaagtcag tagataatat cttacattta aaagtcaaga
     1441 aataacagtc tgaccatact atctagaaat atggaagtta acagtaagga agttaaaaca
     1501 gttgaaagta gtttcgtcta gaaagaaaga cttggggaag aacaaagaag gctaattttc
     1561 attatgagct ttattgtaac agaaaagaga ccactaactc agctaggttt tcccaattcc
     1621 tcaacacttt ggggcacagt gtttagtcag agctgtgatc actctctgag cacatggatt
     1681 gtatcttaat tttcatctca ttgaaaatat gtaccatttg tccaagtgga aactgggctg
     1741 aagcatgact gtgaccacca ccaaagaaat ggctagactc gctaaggtct gctggggtga
     1801 cccgtgattg tgttgtgctt gatcttgttg agcatggctc ttttaagtca ccatacctgc
     1861 ccctgccaat gcaccaaaca ccaacttaaa gggactttca gcttttgtaa gtcagtctca
     1921 gccttacttc tgaaccattt ctttgacttc tttcatgact ctgatcctgt cccagtttta
     1981 agcctcccag ccaccagtca tgctgttttg ctctgctatt tacattcttc tctacctact
     2041 ttggacttga tggtatgcct actatattag ttatttattg ctgtgtaaca aaccactctg
     2101 aaacatagtg gcttacaacc acaaccattt tattttatta tgaaactgtg ggtcagaatg
     2161 tatattggcc cattggcatg attctcctgc tgcggccgcg gcgtaatcat ggtcatagct
     2221 gtttcctgtg tgaaattgtt atccgctcac aattccacac aacatacgag ccggaagcat
     2281 aaagtgtaaa gcctggggtg cctaatgagt gagctaactc acattaattg cgttgcgctc
     2341 actgcccgct ttccagtcgg gaaacctgtc gtgccagctg cattaatgaa tcggccaacg
     2401 cgcggggaga ggcggtttgc gtattgggcg ctcttccgct tcctcgctca ctgactcgct
     2461 gcgctcggtc gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg taatacggtt
     2521 atccacagaa tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc
     2581 caggaaccgt aaaaaggccg cgttgctggc gtttttccat aggctccgcc cccctgacga
     2641 gcatcacaaa aatcgacgct caagtcagag gtggcgaaac ccgacaggac tataaagata
     2701 ccaggcgttt ccccctggaa gctccctcgt gcgctctcct gttccgaccc tgccgcttac
     2761 cggatacctg tccgcctttc tcccttcggg aagcgtggcg ctttctcata gctcacgctg
     2821 taggtatctc agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc
     2881 cgttcagccc gaccgctgcg ccttatccgg taactatcgt cttgagtcca acccggtaag
     2941 acacgactta tcgccactgg cagcagccac tggtaacagg attagcagag cgaggtatgt
     3001 aggcggtgct acagagttct tgaagtggtg gcctaactac ggctacacta gaagaacagt
     3061 atttggtatc tgcgctctgc tgaagccagt taccttcgga aaaagagttg gtagctcttg
     3121 atccggcaaa caaaccaccg ctggtagcgg tggttttttt gtttgcaagc agcagattac
     3181 gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt tctacggggt ctgacgctca
     3241 gtggaacgaa aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac
     3301 ctagatcctt ttaaattaaa aatgaagttt taaatcaagc ccaatctgaa taatgttaca
     3361 accaattaac caattctgat tagaaaaact catcgagcat caaatgaaac tgcaatttat
     3421 tcatatcagg attatcaata ccatattttt gaaaaagccg tttctgtaat gaaggagaaa
     3481 actcaccgag gcagttccat aggatggcaa gatcctggta tcggtctgcg attccgactc
     3541 gtccaacatc aatacaacct attaatttcc cctcgtcaaa aataaggtta tcaagtgaga
     3601 aatcaccatg agtgacgact gaatccggtg agaatggcaa aagtttatgc atttctttcc
     3661 agacttgttc aacaggccag ccattacgct cgtcatcaaa atcactcgca tcaaccaaac
     3721 cgttattcat tcgtgattgc gcctgagcga gacgaaatac gcgatcgctg ttaaaaggac
     3781 aattacaaac aggaatcgaa tgcaaccggc gcaggaacac tgccagcgca tcaacaatat
     3841 tttcacctga atcaggatat tcttctaata cctggaatgc tgtttttccg gggatcgcag
     3901 tggtgagtaa ccatgcatca tcaggagtac ggataaaatg cttgatggtc ggaagaggca
     3961 taaattccgt cagccagttt agtctgacca tctcatctgt aacatcattg gcaacgctac
     4021 ctttgccatg tttcagaaac aactctggcg catcgggctt cccatacaag cgatagattg
     4081 tcgcacctga ttgcccgaca ttatcgcgag cccatttata cccatataaa tcagcatcca
     4141 tgttggaatt taatcgcggc ctcgacgttt cccgttgaat atggctcata acaccccttg
     4201 tattactgtt tatgtaagca gacagtttta ttgttcatga tgatatattt ttatcttgtg
     4261 caatgtaaca tcagagattt tgagacacgg gccagagctg catcgcgcgt ttcggtgatg
     4321 acggtgaaaa cctctgacac atgcagctcc cggagacggt cacagcttgt ctgtaagcgg
     4381 atgccgggag cagacaagcc cgtcagggcg cgtcagcggg tgttggcggg tgtcggggct
     4441 ggcttaacta tgcggcatca gagcagattg tactgagagt gcaccatatg cggtgtgaaa
     4501 taccgcacag atgcgtaagg agaaaatacc gcatcaggcg ccattcgcca ttcaggctgc
     4561 gcaactgttg ggaagggcga tcggtgcggg cctcttcgct attacgccag ctggcgaaag
     4621 ggggatgtgc tgcaaggcga ttaagttggg taacgccagg gttttcccag tcacgacgtt
     4681 gtaaaacgac ggccagtgtt taaacggctc tgttggagag tccagtattg aattagtgat
     4741 agatacacag aaaaaaagca aattgaatag gtgaatggat aaacaaactg tggaacatcc
     4801 agacaatgaa atattcacca ataaaaagaa atgagctatc aagccacatt ggctatgccc
     4861 ttcacattta aggaggaacc ttaaatgcat attgccaaat tttaaaagct agtctgaaaa
     4921 tgctacagac tgtatgattc caattatatg acattttaga aaaggcaaaa ctatagagat
     4981 cagtggttgc cagcggctca tggaggcagg atgaattggt ggaacacagg gatgctttca
     5041 gggtaatgaa aatatcttgt gtgatcctgt aataatggat acgtggcata atgcatttgt
     5101 taaaacctgt aaactagcca ggtatagtgg cttttttgca cctatagtct cagctacttg
     5161 gtaggctgag acaggaagat cacttgagcc caggacttca agaccagcct gggtaatata
     5221 gtaagacccc atctctattt tttaaagaaa ttaagaaaga aaagaaaccc atcaactgta
     5281 taacacaaag aatcgtaatg taaactacag tcctgagtaa tgaaacattt ttaaagaaat
     5341 aaataaattc aaggcaatta ttaacttcag gaaaaacagt aaagtaaaca taagtataca
     5401 aatcaaacat ggctcagttg tgaaaatatt tacaaggtca ctttaagtaa cattgaatgt
     5461 tgatataatc ccaaaatata tttatgttgg aaggatgagg agtttaaaca atattctcac
     5521 tgactccgtc ctggagttgg atgagagata atggccttac gttgtgccag gggagggtcg
     5581 ggctggattt agcaagattt accttctcca aagagcggtg ctgcagtggc acagctgccc
     5641 acggaggtgg gggggtcacc gtccctggag gtgatgaaga actgtgggga tgtggcactg
     5701 agggacatgg ccagtgggca cggtgggtgg gttggggttg gtcttgggga tcttggaggg
     5761 cttttccagc cttcatgatt tgacgattgt atgaacatct acatggcaat tctccagctg
     5821 cctgtcccag tcctactgac ccagctgtat ctctccaggc aagctcttcc accccttctg
     5881 cttgcatcca gacaccatca aacatgcagg ctcagacaca gggaccagca gtgtctgtgg
     5941 cctttttgtg ctcctctcca tgctgggttt taacttgctc tttgtccttc tatcctatct
     6001 tcttatcctt aaggctgttc tgaacgctgt gacttggaga gtgtcccaga gccctcaaca
     6061 cctgcatgtc ccacgtccat gctgtcctgc acttccttat ccccaagatc tgcctctccg
     6121 tgatgcactg aattggcaaa catgtgtcac cccagaccaa caatgtcaca gcaaactccc
     6181 ccttgatagg acaaggggga atggctttac actgagacag gggaggtttg ggttggatat
     6241 gaggaggcag tttttccccc agagggtggt gacgcactga acaggttgcc caaggaggct
     6301 gtggatgccc catccctgca ggcattcaag gccaggctgg atgtggctct gggcagcctg
     6361 ggctgctggt tgatgaccct gcacatagca gggggttgga tctggatgag cactgtgctc
     6421 ctttgcaacc caggccgttc tatgattctg tcattctaaa tctctctttc agcctaaagc
     6481 tttttccccg tatcccccca ggtgtctgca ggctcaaaga gcagcgagaa gcgttcagag
     6541 gaaagcgatc ccgtgccacc ttccccgtgc ccgggctgtc cccgcacgct gccggctcgg
     6601 ggatgcgggg ggagcgccgg accggagcgg agccccgggc ggctcgctgc tgccccctag
     6661 cgggggaggg acgtaattac atccctgggg gctttggggg ggggctgtcc ccgtgagctc
     6721 agtactatgc atgttaactt aattaaggta cccgacttaa gagtactgtc aaggcagtga
     6781 aaaaaatgct ttatttgtga aatttgtgat gctattgctt tatttgtaac cattataagc
     6841 tgcaataaac aagttaacaa caacaattgc attcatttta tgtttcaggt tcagggggag
     6901 gtgtgggagg ttttttaaag caagtaaaac ctctacaaat gtggtatggc tgattatgat
     6961 cctctagaga tatcgtcgac aagcttatcg atgcggccgc gctagcacgc gtcagctgac
     7021 tagaggatcc ccgggtaccg agctcgaatt cttacttgta cagctcgtcc atgccgccgg
     7081 tggagtggcg gccctcggcg cgttcgtact gttccacgat ggtgtagtcc tcgttgtggg
     7141 aggtgatgtc caacttgatg ttgacgttgt aggcgccggg cagctgcacg ggcttcttgg
     7201 ccttgtaggt ggtcttgacc tcagcgtcgt agtggccgcc gtccttcagc ttcagcctct
     7261 gcttgatctc gcccttcagg gcgccgtcct cggggtacat ccgctcggag gaggcctccc
     7321 agcccatggt cttcttctgc attacggggc cgtcggaggg gaagttggtg ccgcgcagct
     7381 tcaccttgta gatgaactcg ccgtcctgca gggaggagtc ctgggtcacg gtcaccacgc
     7441 cgccgtcctc gaagttcatc acgcgctccc acttgaagcc ctcggggaag gacagcttca
     7501 agtagtcggg gatgtcggcg gggtgcttca cgtaggcctt ggagccgtac atgaactgag
     7561 gggacaggat gtcccaggcg aagggcaggg ggccaccctt ggtcaccttc agcttggcgg
     7621 tctgggtgcc ctcgtagggg cggccctcgc cctcgccctc gatctcgaac tcgtggccgt
     7681 tcacggagcc ctccatgtgc accttgaagc gcatgaactc cttgatgatg gccatgttat
     7741 cctcctcgcc cttgctcacc atggtggcac tagtaagtat aagacaaaag tgttgtggaa
     7801 ttgctccagg cgatctgacg gttcactaaa cgagctctgc ttttataggc gcccaccgta
     7861 cacgcctacc tcgacatacg ttctctatca ctgataggga gtaaactgga tatacgttct
     7921 ctatcactga tagggagtaa actgtagata cgttctctat cactgatagg gagtaaactg
     7981 gtcatacgtt ctctatcact gatagggagt aaactcctta tacgttctct atcactgata
     8041 gggagtaaag tctgcatacg ttctctatca ctgataggga gtaaactctt catacgttct
     8101 ctatcactga tagggagtaa actcgagtga ccatagagcc caccggcgcg ccaatattct
     8161 cactgactcc gtcctggagt tggatgagag ataatggcct tacgttgtgc caggggaggg
     8221 tcgggctgga tttagcaaga tttaccttct ccaaagagcg gtgctgcagt ggcacagc
//
