LOCUS       sequence-457007-        7406 bp    DNA     circular SYN 17-SEP-2025
DEFINITION  AAV packaging plasmid, expressing Rep2 and Cap2.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7406)
  TITLE     AAV Packaging
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7406)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Sep 17, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pAAV2/2
FEATURES             Location/Qualifiers
     source          1..7406
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             3723..3734
                     /codon_start=1
                     /product="WELQut protease recognition and cleavage site"
                     /label=WELQut site
                     /translation="WELQ"
     primer_bind     complement(4037..4053)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4037..4053)
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     primer_bind     complement(4269..4289)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(4269..4287)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(4308..4324)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4308..4324)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(4321..4343)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    4332..4348
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(4356..4362,4363..4380,4381..4386))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    4401..4422
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(4539..4556)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(4710..5298)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(4790..4809)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(5469..6260,6261..6329))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     6092..6111
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(6330..6434)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(6460..6915)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6597..6618)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     6809..6828
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     7042..7064
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     7056..7073
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     7057..7073
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     7083..7102
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        7083..7101
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     7126..7142
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     primer_bind     7127..7143
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 gagtaaggcc ccggaggctc ttttctttgt gcaatttgag aagggagaga gctacttcca
       61 catgcacgtg ctcgtggaaa ccaccggggt gaaatccatg gttttgggac gtttcctgag
      121 tcagattcgc gaaaaactga ttcagagaat ttaccgcggg atcgagccga ctttgccaaa
      181 ctggttcgcg gtcacaaaga ccagaaatgg cgccggaggc gggaacaagg tggtggatga
      241 gtgctacatc cccaattact tgctccccaa aacccagcct gagctccagt gggcgtggac
      301 taatatggaa cagtatttaa gcgcctgttt gaatctcacg gagcgtaaac ggttggtggc
      361 gcagcatctg acgcacgtgt cgcagacgca ggagcagaac aaagagaatc agaatcccaa
      421 ttctgatgcg ccggtgatca gatcaaaaac ttcagccagg tacatggagc tggtcgggtg
      481 gctcgtggac aaggggatta cctcggagaa gcagtggatc caggaggacc aggcctcata
      541 catctccttc aatgcggcct ccaactcgcg gtcccaaatc aaggctgcct tggacaatgc
      601 gggaaagatt atgagcctga ctaaaaccgc ccccgactac ctggtgggcc agcagcccgt
      661 ggaggacatt tccagcaatc ggatttataa aattttggaa ctaaacgggt acgatcccca
      721 atatgcggct tccgtctttc tgggatgggc cacgaaaaag ttcggcaaga ggaacaccat
      781 ctggctgttt gggcctgcaa ctaccgggaa gaccaacatc gcggaggcca tagcccacac
      841 tgtgcccttc tacgggtgcg taaactggac caatgagaac tttcccttca acgactgtgt
      901 cgacaagatg gtgatctggt gggaggaggg gaagatgacc gccaaggtcg tggagtcggc
      961 caaagccatt ctcggaggaa gcaaggtgcg cgtggaccag aaatgcaagt cctcggccca
     1021 gatagacccg actcccgtga tcgtcacctc caacaccaac atgtgcgccg tgattgacgg
     1081 gaactcaacg accttcgaac accagcagcc gttgcaagac cggatgttca aatttgaact
     1141 cacccgccgt ctggatcatg actttgggaa ggtcaccaag caggaagtca aagacttttt
     1201 ccggtgggca aaggatcacg tggttgaggt ggagcatgaa ttctacgtca aaaagggtgg
     1261 agccaagaaa agacccgccc ccagtgacgc agatataagt gagcccaaac gggtgcgcga
     1321 gtcagttgcg cagccatcga cgtcagacgc ggaagcttcg atcaactacg cagacaggta
     1381 ccaaaacaaa tgttctcgtc acgtgggcat gaatctgatg ctgtttccct gcagacaatg
     1441 cgagagaatg aatcagaatt caaatatctg cttcactcac ggacagaaag actgtttaga
     1501 gtgctttccc gtgtcagaat ctcaacccgt ttctgtcgtc aaaaaggcgt atcagaaact
     1561 gtgctacatt catcatatca tgggaaaggt gccagacgct tgcactgcct gcgatctggt
     1621 caatgtggat ttggatgact gcatctttga acaataaatg atttaaatca ggtatggctg
     1681 ccgatggtta tcttccagat tggctcgagg acactctctc tgaaggaata agacagtggt
     1741 ggaagctcaa acctggccca ccaccaccaa agcccgcaga gcggcataag gacgacagca
     1801 ggggtcttgt gcttcctggg tacaagtacc tcggaccctt caacggactc gacaagggag
     1861 agccggtcaa cgaggcagac gccgcggccc tcgagcacga caaagcctac gaccggcagc
     1921 tcgacagcgg agacaacccg tacctcaagt acaaccacgc cgacgcggag tttcaggagc
     1981 gccttaaaga agatacgtct tttgggggca acctcggacg agcagtcttc caggcgaaaa
     2041 agagggttct tgaacctctg ggcctggttg aggaacctgt taagacggct ccgggaaaaa
     2101 agaggccggt agagcactct cctgtggagc cagactcctc ctcgggaacc ggaaaggcgg
     2161 gccagcagcc tgcaagaaaa agattgaatt ttggtcagac tggagacgca gactcagtac
     2221 ctgaccccca gcctctcgga cagccaccag cagccccctc tggtctggga actaatacga
     2281 tggctacagg cagtggcgca ccaatggcag acaataacga gggcgccgac ggagtgggta
     2341 attcctcggg aaattggcat tgcgattcca catggatggg cgacagagtc atcaccacca
     2401 gcacccgaac ctgggccctg cccacctaca acaaccacct ctacaaacaa atttccagcc
     2461 aatcaggagc ctcgaacgac aatcactact ttggctacag caccccttgg gggtattttg
     2521 acttcaacag attccactgc cacttttcac cacgtgactg gcaaagactc atcaacaaca
     2581 actggggatt ccgacccaag agactcaact tcaagctctt taacattcaa gtcaaagagg
     2641 tcacgcagaa tgacggtacg acgacgattg ccaataacct taccagcacg gttcaggtgt
     2701 ttactgactc ggagtaccag ctcccgtacg tcctcggctc ggcgcatcaa ggatgcctcc
     2761 cgccgttccc agcagacgtc ttcatggtgc cacagtatgg atacctcacc ctgaacaacg
     2821 ggagtcaggc agtaggacgc tcttcatttt actgcctgga gtactttcct tctcagatgc
     2881 tgcgtaccgg aaacaacttt accttcagct acacttttga ggacgttcct ttccacagca
     2941 gctacgctca cagccagagt ctggaccgtc tcatgaatcc tctcatcgac cagtacctgt
     3001 attacttgag cagaacaaac actccaagtg gaaccaccac gcagtcaagg cttcagtttt
     3061 ctcaggccgg agcgagtgac attcgggacc agtctaggaa ctggcttcct ggaccctgtt
     3121 accgccagca gcgagtatca aagacatctg cggataacaa caacagtgaa tactcgtgga
     3181 ctggagctac caagtaccac ctcaatggca gagactctct ggtgaatccg ggcccggcca
     3241 tggcaagcca caaggacgat gaagaaaagt tttttcctca gagcggggtt ctcatctttg
     3301 ggaagcaagg ctcagagaaa acaaatgtgg acattgaaaa ggtcatgatt acagacgaag
     3361 aggaaatcag gacaaccaat cccgtggcta cggagcagta tggttctgta tctaccaacc
     3421 tccagagagg caacagacaa gcagctaccg cagatgtcaa cacacaaggc gttcttccag
     3481 gcatggtctg gcaggacaga gatgtgtacc ttcaggggcc catctgggca aagattccac
     3541 acacggacgg acattttcac ccctctcccc tcatgggtgg attcggactt aaacaccctc
     3601 ctccacagat tctcatcaag aacaccccgg tacctgcgaa tccttcgacc accttcagtg
     3661 cggcaaagtt tgcttccttc atcacacagt actccacggg acaggtcagc gtggagatcg
     3721 agtgggagct gcagaaggaa aacagcaaac gctggaatcc cgaaattcag tacacttcca
     3781 actacaacaa gtctgttaat gtggacttta ctgtggacac taatggcgtg tattcagagc
     3841 ctcgccccat tggcaccaga tacctgactc gtaatctgta attacgtgtt aatcaataaa
     3901 ccggttgatt cgtttcagtt gaactttggt ctcctgtcct tcttatctta tcggttacca
     3961 tggttatagc ttacacatta actgcttggt tgcgcttcgc gataaaagac ttacgtcatc
     4021 gggttacccc tagggggatc cactagttct agaggtcctg tattagaggt cacgtgagtg
     4081 ttttgcgaca ttttgcgaca ccatgtggtc acgctgggta tttaagcccg agtgagcacg
     4141 cagggtctcc attttgaagc gggaggtttg aacgcgcagc cgccaagccg aattctgcag
     4201 atatccatca cactggcggc cgctcgacta gagcggccgc caccgcggtg gagctccagc
     4261 ttttgttccc tttagtgagg gttaattgcg cgcttggcgt aatcatggtc atagctgttt
     4321 cctgtgtgaa attgttatcc gctcacaatt ccacacaaca tacgagccgg aagcataaag
     4381 tgtaaagcct ggggtgccta atgagtgagc taactcacat taattgcgtt gcgctcactg
     4441 cccgctttcc agtcgggaaa cctgtcgtgc cagctgcatt aatgaatcgg ccaacgcgcg
     4501 gggagaggcg gtttgcgtat tgggcgctct tccgcttcct cgctcactga ctcgctgcgc
     4561 tcggtcgttc ggctgcggcg agcggtatca gctcactcaa aggcggtaat acggttatcc
     4621 acagaatcag gggataacgc aggaaagaac atgtgagcaa aaggccagca aaaggccagg
     4681 aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc tccgcccccc tgacgagcat
     4741 cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga caggactata aagataccag
     4801 gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga
     4861 tacctgtccg cctttctccc ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg
     4921 tatctcagtt cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga accccccgtt
     4981 cagcccgacc gctgcgcctt atccggtaac tatcgtcttg agtccaaccc ggtaagacac
     5041 gacttatcgc cactggcagc agccactggt aacaggatta gcagagcgag gtatgtaggc
     5101 ggtgctacag agttcttgaa gtggtggcct aactacggct acactagaag aacagtattt
     5161 ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa gagttggtag ctcttgatcc
     5221 ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt gcaagcagca gattacgcgc
     5281 agaaaaaaag gatctcaaga agatcctttg atcttttcta cggggtctga cgctcagtgg
     5341 aacgaaaact cacgttaagg gattttggtc atgagattat caaaaaggat cttcacctag
     5401 atccttttaa attaaaaatg aagttttaaa tcaatctaaa gtatatatga gtaaacttgg
     5461 tctgacagtt accaatgctt aatcagtgag gcacctatct cagcgatctg tctatttcgt
     5521 tcatccatag ttgcctgact ccccgtcgtg tagataacta cgatacggga gggcttacca
     5581 tctggcccca gtgctgcaat gataccgcga gacccacgct caccggctcc agatttatca
     5641 gcaataaacc agccagccgg aagggccgag cgcagaagtg gtcctgcaac tttatccgcc
     5701 tccatccagt ctattaattg ttgccgggaa gctagagtaa gtagttcgcc agttaatagt
     5761 ttgcgcaacg ttgttgccat tgctacaggc atcgtggtgt cacgctcgtc gtttggtatg
     5821 gcttcattca gctccggttc ccaacgatca aggcgagtta catgatcccc catgttgtgc
     5881 aaaaaagcgg ttagctcctt cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg
     5941 ttatcactca tggttatggc agcactgcat aattctctta ctgtcatgcc atccgtaaga
     6001 tgcttttctg tgactggtga gtactcaacc aagtcattct gagaatagtg tatgcggcga
     6061 ccgagttgct cttgcccggc gtcaatacgg gataataccg cgccacatag cagaacttta
     6121 aaagtgctca tcattggaaa acgttcttcg gggcgaaaac tctcaaggat cttaccgctg
     6181 ttgagatcca gttcgatgta acccactcgt gcacccaact gatcttcagc atcttttact
     6241 ttcaccagcg tttctgggtg agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata
     6301 agggcgacac ggaaatgttg aatactcata ctcttccttt ttcaatatta ttgaagcatt
     6361 tatcagggtt attgtctcat gagcggatac atatttgaat gtatttagaa aaataaacaa
     6421 ataggggttc cgcgcacatt tccccgaaaa gtgccaccta aattgtaagc gttaatattt
     6481 tgttaaaatt cgcgttaaat ttttgttaaa tcagctcatt ttttaaccaa taggccgaaa
     6541 tcggcaaaat cccttataaa tcaaaagaat agaccgagat agggttgagt gttgttccag
     6601 tttggaacaa gagtccacta ttaaagaacg tggactccaa cgtcaaaggg cgaaaaaccg
     6661 tctatcaggg cgatggccca ctacgtgaac catcacccta atcaagtttt ttggggtcga
     6721 ggtgccgtaa agcactaaat cggaacccta aagggagccc ccgatttaga gcttgacggg
     6781 gaaagccggc gaacgtggcg agaaaggaag ggaagaaagc gaaaggagcg ggcgctaggg
     6841 cgctggcaag tgtagcggtc acgctgcgcg taaccaccac acccgccgcg cttaatgcgc
     6901 cgctacaggg cgcgtcccat tcgccattca ggctgcgcaa ctgttgggaa gggcgatcgg
     6961 tgcgggcctc ttcgctatta cgccagctgg cgaaaggggg atgtgctgca aggcgattaa
     7021 gttgggtaac gccagggttt tcccagtcac gacgttgtaa aacgacggcc agtgagcgcg
     7081 cgtaatacga ctcactatag ggcgaattgg gtaccgggcc ccccctcgag gtcgacggta
     7141 tcgggggagc tcgcagggtc tccattttga agcgggaggt ttgaacgcgc agccgccatg
     7201 ccggggtttt acgagattgt gattaaggtc cccagcgacc ttgacgagca tctgcccggc
     7261 atttctgaca gctttgtgaa ctgggtggcc gagaaggaat gggagttgcc gccagattct
     7321 gacatggatc tgaatctgat tgagcaggca cccctgaccg tggccgagaa gctgcagcgc
     7381 gactttctga cggaatggcg ccgtgt
//
