LOCUS       sequence-457059-       13299 bp    DNA     circular SYN 15-APR-2025
DEFINITION  For targeted tethering of an inactive mutant (HxD) of the catalytic 
            domain of mouse TET2 using dCas13.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 13299)
  AUTHORS   Zou Z, Dou X, Li Y, Zhang Z, Wang J, Gao B, Xiao Y, Wang Y, Zhao L, 
            Sun C, Liu Q, Yu X, Wang H, Hong J, Dai Q, Yang FC, Xu M, He C
  TITLE     RNA m(5)C oxidation by TET2 regulates chromatin state and 
            leukaemogenesis.
  JOURNAL   Nature. 2024 Oct 2. doi: 10.1038/s41586-024-07969-x.
  PUBMED    39358506
REFERENCE   2  (bases 1 to 13299)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nature. 
            2024 Oct 2. doi: 10.1038/s41586-024-07969-x."
COMMENT     Sequence Label: pB-3xFlag-dCas13-mTET2HxDCD
FEATURES             Location/Qualifiers
     source          1..13299
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             16..36
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     protein_bind    complement(5629..5653)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     primer_bind     complement(5674..5690)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    5698..5714
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(5722..5728,5729..5746,5747..5752))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    5767..5788
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(5848..5865)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    5854..6078
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     protein_bind    6155..6188
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     repeat_region   6359..6421
                     /label=piggyBac right (3') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
     promoter        complement(6445..6463)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(6484..6500)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    6508..6524
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(6532..6538,6539..6556,6557..6562))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6577..6598
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(6715..6732)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(6886..7474)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6966..6985)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(7645..8436,8437..8505))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8268..8287
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8506..8610)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(8636..9091)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(8773..8794)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     8985..9004
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     9218..9240
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     9232..9249
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     9233..9249
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     9256..9275
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        9256..9274
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     repeat_region   9293..9327
                     /label=piggyBac left (5') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
     CDS             complement(9809..10834)
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     CDS             complement(9809..10834)
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     promoter        complement(10853..11352)
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(10901..10920)
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     primer_bind     11294..11317
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     protein_bind    11375..11408
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     enhancer        11424..11803
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     intron          12105..13120
                     /label=chimeric intron
                     /note="chimera between introns from chicken beta-actin and 
                     rabbit beta-globin"
     primer_bind     13044..13066
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
     primer_bind     13128..13147
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     protein_bind    13198..13222
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             13231..13296
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
ORIGIN
        1 tccggcgccg ctagcccaaa gaagaagcgg aaagtcaaca tccccgctct ggtggaaaac
       61 cagaagaagt actttggcac ctacagcgtg atggccatgc tgaacgctca gaccgtgctg
      121 gaccacatcc agaaggtggc cgatattgag ggcgagcaga acgagaacaa cgagaatctg
      181 tggtttcacc ccgtgatgag ccacctgtac aacgccaaga acggctacga caagcagccc
      241 gagaaaacca tgttcatcat cgagcggctg cagagctact tcccattcct gaagatcatg
      301 gccgagaacc agagagagta cagcaacggc aagtacaagc agaaccgcgt ggaagtgaac
      361 agcaacgaca tcttcgaggt gctgaagcgc gccttcggcg tgctgaagat gtacagggac
      421 ctgaccaacg catacaagac ctacgaggaa aagctgaacg acggctgcga gttcctgacc
      481 agcacagagc aacctctgag cggcatgatc aacaactact acacagtggc cctgcggaac
      541 atgaacgaga gatacggcta caagacagag gacctggcct tcatccagga caagcggttc
      601 aagttcgtga aggacgccta cggcaagaaa aagtcccaag tgaataccgg attcttcctg
      661 agcctgcagg actacaacgg cgacacacag aagaagctgc acctgagcgg agtgggaatc
      721 gccctgctga tctgcctgtt cctggacaag cagtacatca acatctttct gagcaggctg
      781 cccatcttct ccagctacaa tgcccagagc gaggaacggc ggatcatcat cagatccttc
      841 ggcatcaaca gcatcaagct gcccaaggac cggatccaca gcgagaagtc caacaagagc
      901 gtggccatgg atatgctcaa cgaagtgaag cggtgccccg acgagctgtt cacaacactg
      961 tctgccgaga agcagtcccg gttcagaatc atcagcgacg accacaatga agtgctgatg
     1021 aagcggagca gcgacagatt cgtgcctctg ctgctgcagt atatcgatta cggcaagctg
     1081 ttcgaccaca tcaggttcca cgtgaacatg ggcaagctga gatacctgct gaaggccgac
     1141 aagacctgca tcgacggcca gaccagagtc agagtgatcg agcagcccct gaacggcttc
     1201 ggcagactgg aagaggccga gacaatgcgg aagcaagaga acggcacctt cggcaacagc
     1261 ggcatccgga tcagagactt cgagaacatg aagcgggacg acgccaatcc tgccaactat
     1321 ccctacatcg tggacaccta cacacactac atcctggaaa acaacaaggt cgagatgttt
     1381 atcaacgaca aagaggacag cgccccactg ctgcccgtga tcgaggatga tagatacgtg
     1441 gtcaagacaa tccccagctg ccggatgagc accctggaaa ttccagccat ggccttccac
     1501 atgtttctgt tcggcagcaa gaaaaccgag aagctgatcg tggacgtgca caaccggtac
     1561 aagagactgt tccaggccat gcagaaagaa gaagtgaccg ccgagaatat cgccagcttc
     1621 ggaatcgccg agagcgacct gcctcagaag atcctggatc tgatcagcgg caatgcccac
     1681 ggcaaggatg tggacgcctt catcagactg accgtggacg acatgctgac cgacaccgag
     1741 cggagaatca agagattcaa ggacgaccgg aagtccattc ggagcgccga caacaagatg
     1801 ggaaagagag gcttcaagca gatctccaca ggcaagctgg ccgacttcct ggccaaggac
     1861 atcgtgctgt ttcagcccag cgtgaacgat ggcgagaaca agatcaccgg cctgaactac
     1921 cggatcatgc agagcgccat tgccgtgtac gatagcggcg acgattacga ggccaagcag
     1981 cagttcaagc tgatgttcga gaaggcccgg ctgatcggca agggcacaac agagcctcat
     2041 ccatttctgt acaaggtgtt cgcccgcagc atccccgcca atgccgtcga gttctacgag
     2101 cgctacctga tcgagcggaa gttctacctg accggcctgt ccaacgagat caagaaaggc
     2161 aacagagtgg atgtgccctt catccggcgg gaccagaaca agtggaaaac acccgccatg
     2221 aagaccctgg gcagaatcta cagcgaggat ctgcccgtgg aactgcccag acagatgttc
     2281 gacaatgaga tcaagtccca cctgaagtcc ctgccacaga tggaaggcat cgacttcaac
     2341 aatgccaacg tgacctatct gatcgccgag tacatgaaga gagtgctgga cgacgacttc
     2401 cagaccttct accagtggaa ccgcaactac cggtacatgg acatgcttaa gggcgagtac
     2461 gacagaaagg gctccctgca gcactgcttc accagcgtgg aagagagaga aggcctctgg
     2521 aaagagcggg cctccagaac agagcggtac agaaagcagg ccagcaacaa gatccgcagc
     2581 aaccggcaga tgagaaacgc cagcagcgaa gagatcgaga caatcctgga taagcggctg
     2641 agcaacagcc ggaacgagta ccagaaaagc gagaaagtga tccggcgcta cagagtgcag
     2701 gatgccctgc tgtttctgct ggccaaaaag accctgaccg aactggccga tttcgacggc
     2761 gagaggttca aactgaaaga aatcatgccc gacgccgaga agggaatcct gagcgagatc
     2821 atgcccatga gcttcacctt cgagaaaggc ggcaagaagt acaccatcac cagcgagggc
     2881 atgaagctga agaactacgg cgacttcttt gtgctggcta gcgacaagag gatcggcaac
     2941 ctgctggaac tcgtgggcag cgacatcgtg tccaaagagg atggatccaa aagaaccgcc
     3001 gacggcagcg aattcggcaa atgtgaagga tgcaatccag acaaagatga agctccttat
     3061 tatacccatc taggagctgg tcctgatgtg gcagctatta gaacactcat ggaagaaagg
     3121 tatggagaga agggtaaagc tattaggatt gaaaaagtca tatatactgg taaagaaggc
     3181 aagagctctc agggatgtcc tattgctaaa tgggtatatc ggagatcgag tgaggaggag
     3241 aaactactgt gtttggtacg agtgcgacct aatcacacat gtgagacggc ggtgatggta
     3301 attgccatca tgttgtggga cggaatccca aagctactcg catcagaact ctactcagaa
     3361 cttacagata tcttgggcaa gtgtggcata tgcaccaacc gtcgctgttc tcagaatgaa
     3421 actagaaact gttgttgtca gggtgagaat ccagagacct gtggtgcctc cttttctttt
     3481 ggttgttctt ggagcatgta ctataatgga tgtaagtttg ccagaagcaa gaaaccaagg
     3541 aaatttaggc tacatggagc tgagccaaaa gaggaagaga gactaggttc tcatttgcaa
     3601 aacctggcta ctgtcattgc tccaatatac aagaagcttg cacccgatgc atacaataat
     3661 caggttgaat ttgaacacca agccccagac tgctgtttgg gtctgaagga aggccggcca
     3721 ttctcaggag tcactgcatg tttggacttc tctgctcatt cctacagagc acagcagaac
     3781 atgccaaatg gcagtacagt ggtggtcacc ctcaatagag aagacaatcg agaagtcgga
     3841 gctaagcctg aggatgagca gttccacgtg ctgcctatgt acatcatcgc ccctgaggat
     3901 gagtttggga gtacggaagg ccaggagaag aagatacgga tggggtccat tgaggttctg
     3961 cagtcatttc ggaggagaag ggtcataagg ataggagagc tgcccaagag ttgcaagaag
     4021 aaagcggagc ccaagaaagc caagaccaag aaagcagctc gaaagcgttc ctctctggag
     4081 aactgctcca gtaggactga gaagggaaag tcttcctcac atacaaagct gatggaaaat
     4141 gcaagccata tgaaacaaat gacagcacaa ccgcagcttt cgggcccggt catccggcag
     4201 ccaccaacac tccagaggca ccttcagcaa gggcagaggc cacagcagcc gcagccacct
     4261 cagccgcagc cgcagacgac acctcagcca cagccacagc cacagcatat catgcccggt
     4321 aactctcagt ctgttggttc tcattgttct ggatccacca gtgtctacac gagacagcct
     4381 actcctcaca gtccttatcc cagctcagca cacacctcag atatttatgg agataccaac
     4441 catgtgaact tttaccccac ttcatctcat gcctcgggtt catatttgaa tccttctaat
     4501 tacatgaacc cctaccttgg gcttttgaat cagaataacc aatatgcacc ttttccatac
     4561 aatgggagtg tgccagtgga caatggttcc cctttcttag gttcttattc cccccaggct
     4621 cagtccaggg atctacatag atatccaaac caggaccatc tcaccaatca gaacttacca
     4681 cccatccaca cccttcacca acagacgttt ggggacagtc cctctaagta cttaagttat
     4741 ggaaaccaaa atatgcagag agatgccttc actactaact ccaccctaaa accaaatgta
     4801 caccacctag caacgttttc tccttacccc acccccaaga tggatagtca tttcatggga
     4861 gctgcctcca gatcaccata cagccaccca cacactgact acaaaaccag tgagcatcat
     4921 ctaccctctc acacgatcta cagctacacg gcagcagctt cggggagcag ttccagccac
     4981 gccttccaca acaaggagaa tgacaacata gccaatgggc tctcaagagt gcttccaggg
     5041 tttaatcatg atagaactgc ttctgcccaa gaactattat acagtctgac tggcagcagt
     5101 caggagaagc agcctgaggt gtcaggccag gatgcagctg ctgtgcagga aattgagtat
     5161 tggtcagata gtgagcacaa ctttcaggat ccttgcattg gaggggtggc tatagctcca
     5221 actcatgggt caattcttat tgagtgtgca aagtgtgagg ttcatgccac aaccaaagta
     5281 aacgatcccg accggaatca ccccaccagg atctcacttg tactgtatag gcataagaat
     5341 ttgtttctac caaaacattg tttggctctc tgggaagcca aaatggctga aaaggcccgg
     5401 aaagaggaag agtgcggaaa gaatggatca gaccacgtgt ctcagaaaaa tcatggcaaa
     5461 caggaaaagc gtgagcccac agggccacag gaacccagtt acctgcgttt catccagtct
     5521 cttgctgaga acacagggtc tgtgactacg gattctaccg tgactacatc accatatgct
     5581 ttcactcagg tcacagggcc ttacaacaca tttgtatgat taattaagac ccagctttct
     5641 tgtacaaagt ggtgatagct tggcgtaatc atggtcatag ctgtttcctg tgtgaaattg
     5701 ttatccgctc acaattccac acaacatacg agccggaagc ataaagtgta aagcctgggg
     5761 tgcctaatga gtgagctaac tcacattaat tgcgttgcgc tcactgcccg ctttccagtc
     5821 gggaaacctg tcgtgccagc ggatcagcct cgactgtgcc ttctagttgc cagccatctg
     5881 ttgtttgccc ctcccccgtg ccttccttga ccctggaagg tgccactccc actgtccttt
     5941 cctaataaaa tgaggaaatt gcatcgcatt gtctgagtag gtgtcattct attctggggg
     6001 gtggggtggg gcaggacagc aagggggagg attgggaaga caatagcagg catgctgggg
     6061 atgcggtggg ctctatggct tctgaggcgg aaagaaccag ctggggctcg agatccacta
     6121 gttctagcct cgaggctaga gcggccgcgt cgagataact tcgtataatg tatgctatac
     6181 gaagttatgt tttgttactt tatagaagaa attttgagtt tttgtttttt tttaataaat
     6241 aaataaacat aaataaattg tttgttgaat ttattattag tatgtaagtg taaatataat
     6301 aaaacttaat atctattcaa attaataaat aaacctcgat atacagaccg ataaaacaca
     6361 tgcgtcaatt ttacgcatga ttatctttaa cgtacgtcac aatatgatta tctttctagg
     6421 gttaaagagc tccagctttt gttcccttta gtgagggtta atttcgagct tggcgtaatc
     6481 atggtcatag ctgtttcctg tgtgaaattg ttatccgctc acaattccac acaacatacg
     6541 agccggaagc ataaagtgta aagcctgggg tgcctaatga gtgagctaac tcacattaat
     6601 tgcgttgcgc tcactgcccg ctttccagtc gggaaacctg tcgtgccagc tgcattaatg
     6661 aatcggccaa cgcgcgggga gaggcggttt gcgtattggg cgctcttccg cttcctcgct
     6721 cactgactcg ctgcgctcgg tcgttcggct gcggcgagcg gtatcagctc actcaaaggc
     6781 ggtaatacgg ttatccacag aatcagggga taacgcagga aagaacatgt gagcaaaagg
     6841 ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg
     6901 cccccctgac gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg
     6961 actataaaga taccaggcgt ttccccctgg aagctccctc gtgcgctctc ctgttccgac
     7021 cctgccgctt accggatacc tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca
     7081 tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt
     7141 gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc
     7201 caacccggta agacacgact tatcgccact ggcagcagcc actggtaaca ggattagcag
     7261 agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg tggcctaact acggctacac
     7321 tagaagaaca gtatttggta tctgcgctct gctgaagcca gttaccttcg gaaaaagagt
     7381 tggtagctct tgatccggca aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa
     7441 gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat cctttgatct tttctacggg
     7501 gtctgacgct cagtggaacg aaaactcacg ttaagggatt ttggtcatga gattatcaaa
     7561 aaggatcttc acctagatcc ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat
     7621 atatgagtaa acttggtctg acagttacca atgcttaatc agtgaggcac ctatctcagc
     7681 gatctgtcta tttcgttcat ccatagttgc ctgactcccc gtcgtgtaga taactacgat
     7741 acgggagggc ttaccatctg gccccagtgc tgcaatgata ccgcgagacc cacgctcacc
     7801 ggctccagat ttatcagcaa taaaccagcc agccggaagg gccgagcgca gaagtggtcc
     7861 tgcaacttta tccgcctcca tccagtctat taattgttgc cgggaagcta gagtaagtag
     7921 ttcgccagtt aatagtttgc gcaacgttgt tgccattgct acaggcatcg tggtgtcacg
     7981 ctcgtcgttt ggtatggctt cattcagctc cggttcccaa cgatcaaggc gagttacatg
     8041 atcccccatg ttgtgcaaaa aagcggttag ctccttcggt cctccgatcg ttgtcagaag
     8101 taagttggcc gcagtgttat cactcatggt tatggcagca ctgcataatt ctcttactgt
     8161 catgccatcc gtaagatgct tttctgtgac tggtgagtac tcaaccaagt cattctgaga
     8221 atagtgtatg cggcgaccga gttgctcttg cccggcgtca atacgggata ataccgcgcc
     8281 acatagcaga actttaaaag tgctcatcat tggaaaacgt tcttcggggc gaaaactctc
     8341 aaggatctta ccgctgttga gatccagttc gatgtaaccc actcgtgcac ccaactgatc
     8401 ttcagcatct tttactttca ccagcgtttc tgggtgagca aaaacaggaa ggcaaaatgc
     8461 cgcaaaaaag ggaataaggg cgacacggaa atgttgaata ctcatactct tcctttttca
     8521 atattattga agcatttatc agggttattg tctcatgagc ggatacatat ttgaatgtat
     8581 ttagaaaaat aaacaaatag gggttccgcg cacatttccc cgaaaagtgc cacctaaatt
     8641 gtaagcgtta atattttgtt aaaattcgcg ttaaattttt gttaaatcag ctcatttttt
     8701 aaccaatagg ccgaaatcgg caaaatccct tataaatcaa aagaatagac cgagataggg
     8761 ttgagtgttg ttccagtttg gaacaagagt ccactattaa agaacgtgga ctccaacgtc
     8821 aaagggcgaa aaaccgtcta tcagggcgat ggcccactac gtgaaccatc accctaatca
     8881 agttttttgg ggtcgaggtg ccgtaaagca ctaaatcgga accctaaagg gagcccccga
     8941 tttagagctt gacggggaaa gccggcgaac gtggcgagaa aggaagggaa gaaagcgaaa
     9001 ggagcgggcg ctagggcgct ggcaagtgta gcggtcacgc tgcgcgtaac caccacaccc
     9061 gccgcgctta atgcgccgct acagggcgcg tcccattcgc cattcaggct gcgcaactgt
     9121 tgggaagggc gatcggtgcg ggcctcttcg ctattacgcc agctggcgaa agggggatgt
     9181 gctgcaaggc gattaagttg ggtaacgcca gggttttccc agtcacgacg ttgtaaaacg
     9241 acggccagtg aattgtaata cgactcacta tagggcgaat tgggtacctt aaccctagaa
     9301 agatagtctg cgtaaaattg acgcatgcat tcttgaaata ttgctctctc tttctaaata
     9361 gcgcgaatcc gtcgctgtgc atttaggaca tctcagtcgc cgcttggagc tcccgtgagg
     9421 cgtgcttgtc aatgcggtaa gtgtcactga ttttgaacta taacgaccgc gtgagtcaaa
     9481 atgacgcatg attatctttt acgtgacttt taagatttaa ctcatacgat aattatattg
     9541 ttatttcatg ttctacttac gtgataactt attatatata tattttcttg ttatagatat
     9601 ccaactatga aacattatca taaagcaata gtaaagagcc ttcagtaaag agcaggcatt
     9661 tatctaatcc caccccaccc ccacccccgt agctccaatc cttccattca aaatgtaggt
     9721 actctgttct cacccttctt aacaaagtat gacaggaaaa acttccatgt ggacatcttt
     9781 attgtttaat agatcatcaa tttctcgact attcctttgc cctcggacga gtgctggggc
     9841 gtcggtttcc actatcggcg agtacttcta cacagccatc ggtccagacg gccgcgcttc
     9901 tgcgggcgat ttgtgtacgc ccgacagtcc cggctccgga tcggacgatt gcgtcgcatc
     9961 gaccctgcgc ccaagctgca tcatcgaaat tgccgtcaac caagctctga tagagttggt
    10021 caagaccaat gcggagcata tacgcccgga gccgcggcga tcctgcaagc tccggatgcc
    10081 tccgctcgaa gtagcgcgtc tgctgctcca tacaagccaa ccacggcctc cagaagaaga
    10141 tgttggcgac ctcgtattgg gaatccccga acatcgcctc gctccagtca atgaccgctg
    10201 ttatgcggcc attgtccgtc aggacattgt tggagccgaa atccgcgtgc acgaggtgcc
    10261 ggacttcggg gcagtcctcg gcccaaagca tcagctcatc gagagcctgc gcgacggacg
    10321 cactgacggt gtcgtccatc acagtttgcc agtgatacac atggggatca gcaatcgcgc
    10381 atatgaaatc acgccatgta gtgtattgac cgattccttg cggtccgaat gggccgaacc
    10441 cgctcgtctg gctaagatcg gccgcagcga tcgcatccat ggcctccgcg accggctgca
    10501 gaacagcggg cagttcggtt tcaggcaggt cttgcaacgt gacaccctgt gcacggcggg
    10561 agatgcaata ggtcaggctc tcgctgaatt ccccaatgtc aagcacttcc ggaatcggga
    10621 gcgcggccga tgcaaagtgc cgataaacat aacgatcttt gtagaaacca tcggcgcagc
    10681 tatttacccg caggacatat ccacgccctc ctacatcgaa gctgaaagca cgagattctt
    10741 cgccctccga gagctgcatc aggtcggaga cgctgtcgaa cttttcgatc agaaacttct
    10801 cgacagacgt cgcggtgagt tcaggctttt tcatggtggc ggctggatcg gtcgaaaggc
    10861 ccggagatga ggaagaggag aacagcgcgg cagacgtgcg cttttgaagc gtgcagaatg
    10921 ccgggcctcc ggaggacctt cgggcgcccg ccccgcccct gagcccgccc ctgagcccgc
    10981 ccccggaccc accccttccc agcctctgag cccagaaagc gaaggagcaa agctgctatt
    11041 ggccgctgcc ccaaaggcct acccgcttcc attgctcagc ggtgctgtcc atctgcacga
    11101 gactagtgag acgtgctact tccatttgtc acgtcctgca cgacgcgagc tgcggggcgg
    11161 gggggaactt cctgactagg ggaggagtag aaggtggcgc gaaggggcca ccaaagaacg
    11221 gagccggttg gcgcctaccg gtggatgtgg aatgtgtgcg aggccagagg ccacttgtgt
    11281 agcgccaagt gcccagcggg gctgctaaag cgcatgctcc agactgcctt gggaaaagcg
    11341 cctcccctac ccggtagaat tcatcgatga tatcataact tcgtataatg tatgctatac
    11401 gaagttatgc ggccctcgag gtcgacattg attattgact agttattaat agtaatcaat
    11461 tacggggtca ttagttcata gcccatatat ggagttccgc gttacataac ttacggtaaa
    11521 tggcccgcct ggctgaccgc ccaacgaccc ccgcccattg acgtcaataa tgacgtatgt
    11581 tcccatagta acgccaatag ggactttcca ttgacgtcaa tgggtggagt atttacggta
    11641 aactgcccac ttggcagtac atcaagtgta tcatatgcca agtacgcccc ctattgacgt
    11701 caatgacggt aaatggcccg cctggcatta tgcccagtac atgaccttat gggactttcc
    11761 tacttggcag tacatctacg tattagtcat cgctattacc atggtcgagg tgagccccac
    11821 gttctgcttc actctcccca tctccccccc ctccccaccc ccaattttgt atttatttat
    11881 tttttaatta ttttgtgcag cgatgggggc gggggggggg gggggggggc cccaggcggg
    11941 gcgggggggg gggggggggg gggggggggg gggggggggg gggggggggg gggggaagga
    12001 ggggggcggc ggcggccaat cagggcggcg ggctccaaaa gtttcctttt agggcggggg
    12061 gggggcggcg gcggccctat aaaaagcgaa gcgcgcggcg ggcgggagtc gctgcgcgct
    12121 gccttcgccc cgtgccccgc tccgccgccg cctcgcgccg cccgccccgg ctctgactga
    12181 ccgcgttact cccacaggtg agcgggcggg acggcccttc tcctccgggc tgtaattagc
    12241 gcttggttta atgacggctt gtttcttttc tgtggctgcg tgaaagcctt gaggggctcc
    12301 gggagggccc tttgtgcggg gggagcggct cggggggtgc gtgcgtgtgt gtgtgcgtgg
    12361 ggagcgccgc gtgcggctcc gcgctgcccg gcggctgtga gcgctgcggg cgcggcgcgg
    12421 ggctttgtgc gctccgcagt gtgcgcgagg ggagcgcggc cgggggcggt gccccgcggt
    12481 gcgggggggc tgcgagggga acaaaggctg cgtgcggggt gtgtgcgtgg gggggtgagc
    12541 agggggtgtg ggcgcgtcgg tcgggctgca accccccctg cacccccctc cccgagttgc
    12601 tgagcacggc ccggcttcgg gtgcggggct ccgtacgggg cgtggcgcgg ggctcgccgt
    12661 gccgggcggg gggtggcggc aggtgggggt gccgggcggg gcggggccgc ctcgggccgg
    12721 ggagggctcg ggggaggggc gcggcggccc ccggagcgcc ggcggctgtc gaggcgcggc
    12781 gagccgcagc cattgccttt tatggtaatc gtgcgagagg gcgcagggac ttcctttgtc
    12841 ccaaatctgt gcggagccga aatctgggag gcgccgccgc accccctcta gcgggcgcgg
    12901 ggcgaagcgg tgcggcgccg gcaggaagga aatgggcggg gagggccttc gtgcgtcgcc
    12961 gcgccgccgt ccccttctcc ctctccagcc tcggggctgt ccgcgggggg acggctgcct
    13021 tcggggggga cggggcaggg cggggttcgg cttctggcgt gtgaccggcg gctctagagc
    13081 ctctgctaac catgttcatg ccttcttctt tttcctacag ctcctgggca acgtgctggt
    13141 tattgtgctg tctcatcatt ttggcaaaga attcctcgac ggtatcgata agctatcaca
    13201 agtttgtaca aaaaagcagg cttcaccatg gactacaaag accatgacgg tgattataaa
    13261 gatcatgata tcgattacaa ggatgacgat gacaaggga
//
