LOCUS       sequence-457067-        7134 bp    DNA     circular SYN 15-APR-2025
DEFINITION  Mega' with knockout of GvpN and GvpL.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7134)
  AUTHORS   Iburg M, Anderson AP, Wong VT, Anton ED, He A, Lu GJ
  TITLE     Elucidating the assembly of gas vesicles by systematic 
            protein-protein interaction analysis.
  JOURNAL   EMBO J. 2024 Oct;43(19):4156-4172. doi: 10.1038/s44318-024-00178-2. 
            Epub 2024 Sep 3.
  PUBMED    39227754
REFERENCE   2  (bases 1 to 7134)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/s44318-024-00178-2"; journalName: "EMBO J"; date: 
            "2024-10"; volume: "43"; issue: "19"; pages: "4156-4172"
COMMENT     Sequence Label: pST39-Mega'-deltaNL
FEATURES             Location/Qualifiers
     source          1..7134
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     154..171
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    186..325
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(251..270)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     411..433
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     CDS             complement(427..618)
                     /codon_start=1
                     /gene="rop"
                     /product="Rop protein, which maintains plasmids at low copy
                     number"
                     /label=rop
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGENL"
     primer_bind     855..874
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        855..873
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             2324..2341
                     /codon_start=1
                     /product="N-myristoylation signal from Src kinase (Pellman 
                     et al., 1985; Kaplan et al., 1988)"
                     /label=myr
                     /translation="GSSKSK"
     primer_bind     complement(5392..5410)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      5406..5453
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     promoter        5520..5611
                     /gene="bla"
                     /label=AmpR promoter
     terminator      6476..6515
                     /label=fd terminator
                     /note="central terminator from bacteriophage fd (Otsuka and
                     Kunisawa, 1982)"
     rep_origin      6546..7134
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7035..7054
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 aacgccagca acgcggcctt tttacggttc ctggcctttt gctggccttt tgctcacatg
       61 ttctttcctg cgttatcccc tgattctgtg gataaccgta ttaccgcctt tgagtgagct
      121 gataccgctc gccgcagccg aacgaccgag cgcagcgagt cagtgagcga ggaagcggaa
      181 gagcgcctga tgcggtattt tctccttacg catctgtgcg gtatttcaca ccgcaatggt
      241 gcactctcag tacaatctgc tctgatgccg catagttaag ccagtataca ctccgctatc
      301 gctacgtgac tgggtcatgg ctgcgccccg acacccgcca acacccgctg acgcgccctg
      361 acgggcttgt ctgctcccgg catccgctta cagacaagct gtgaccgtct ccgggagctg
      421 catgtgtcag aggttttcac cgtcatcacc gaaacgcgcg aggcagctgc ggtaaagctc
      481 atcagcgtgg tcgtgaagcg attcacagat gtctgcctgt tcatccgcgt ccagctcgtt
      541 gagtttctcc agaagcgtta atgtctggct tctgataaag cgggccatgt taagggcggt
      601 tttttcctgt ttggtcactg atgcctccgt gtaaggggga tttctgttca tggggtaatg
      661 ataccgatga aacgagagag gatgctcacg atacgggtta ctgatgatga acatgcccgg
      721 ttactggaac gttgtgaggg taaacaactg gcggtatgga tgcggcggga ccagagaaaa
      781 atcactcagg gtcaatgcca gcgcttcgtt aatacagatg taggtgttgg caccatgcat
      841 cgatcccgcg aaattaatac gactcactat agggagacca caacggtttc cctctagaga
      901 tagcttagcg atgtgaaaga agctggagaa aactttcaag aaaaagtttc ttcttcagac
      961 gataaagaga agtcaaaaaa taagcggaaa ataaaaggcg taaatcagat taaaaaagca
     1021 acggatatta aaagctctac taagattaaa agttctaatg atattaaatc atctacagat
     1081 ttaaaaacaa tgggatcata agcgaaagta gaaatgagat atgtctattc aaaaaagtac
     1141 taatagttca agtttagcag aagtcattga ccgtatttta gataaaggaa ttgttattga
     1201 tgcttttgca agagtttctg ttgtaggaat tgaaatttta acgattgaag cgcgagtggt
     1261 tattgccagt gttgatacat ggttacgcta tgcagaagca gtagggcttc ttcgtgacga
     1321 cgtagaagaa aacggtcttc ctgaacgttc aaattcaagt gaagggcagc cgcgttttag
     1381 tatttaatat gaagtggtat ggaaccttcc ttttttgagg gtggttccag cttttagatc
     1441 taactattgg aggctactaa aaatggaaat taaaaaaatt atgcaagccg tgaacgactt
     1501 tttcggtgaa cacgtagctc ctcctcataa aattacctcg gtggaagcta ctgaagatga
     1561 aggttggaga gttattgttg aagtcattga agaacgagaa tatatgaaaa aatacgccaa
     1621 agatgaaatg ctcggaacgt acgagtgctt tgtaaataaa gaaaaagaag tcatttcatt
     1681 caaacgactc gacgtcagat atagaagcgc cattggcatt gaagcataaa cgataagatg
     1741 gcgtaaagga gcttgaaaac atgagtgaaa caaacgaaac aggtatttat atttttagcg
     1801 ccattcaaac ggataaagac gaagaatttg gcgccgtgga agtagaagga acaaaagctg
     1861 aaacattttt gattcgctac aaagacgcgg ctatggtagc agctgaagta ccgatgaaaa
     1921 tttatcatcc taatcgccaa aatttattaa tgcatcaaaa cgcagtagca gcgattatgg
     1981 acaagaacga tacggttatt ccaatcagct ttgggaatgt attcaaatca aaagaagacg
     2041 taaaagttct tttggaaaac ctttatccgc agtttgaaaa gctgtttcca gcgatcaaag
     2101 gaaaaattga agtcggttta aaagtaattg ggaaaaaaga atggcttgag aaaaaagtaa
     2161 acgaaaatcc tgaacttgag aaagtatcag catccgtaaa aggaaaatca gaagcagccg
     2221 gttattatga gcgtattcaa cttggaggaa tggctcaaaa gatgtttact tccctgcaaa
     2281 aagaagtcaa gacagatgtg ttttctccgc ttgaagaagc agcggaagca gcaaaagcaa
     2341 atgagccaac gggcgaaacg atgcttttaa acgcgtcttt cttaattaac cgagaagatg
     2401 aagcgaagtt tgatgaaaaa gtaaatgaag cgcatgaaaa ctggaaagac aaagccgatt
     2461 ttcattacag cggtccttgg cctgcttata attttgtgaa cattcgccta aaagtagaag
     2521 agaaataacg tgcttcacaa attagtaacc gcacccatta accttgtagt gaaaatcggc
     2581 gaaaaagtac aggaagaagc tgataaacag ctatatgacc ttccgacgat tcagcaaaag
     2641 ctcattcagc ttcaaatgat gtttgagctt ggtgaaattc cagaagaagc gtttcaagaa
     2701 aaagaagatg aattgttaat gaggtacgaa attgcgaaac gcagagaaat tgaacaatgg
     2761 gaagagctaa cacaaaaaag aaatgaggaa tcctagagtg aggaattaac attatgtctc
     2821 ttaaacaatc catggagaat aaagatattg ctcttattga tattttagat gtcattttag
     2881 ataaaggagt cgccattaaa ggagacttaa tcatttccat agctggcgtc gatttagtgt
     2941 atttggattt gcgggtgctt atttcttcgg ttgaaacgct tgtgcaagca aaagaaggaa
     3001 atcacaaacc aatcacttct gaacaatttg ataaacaaaa agaagagctg atggacgcta
     3061 cgggccagcc tagtaagtgg acgaaccctc taggttcgtg acccttgcga gcagtcgtat
     3121 gctttctcga attccgagcg gttaagcgtg acagtccagg aggaattaat agatgcaacc
     3181 ggtcagccaa gcaaatggac gaatccactt ggatcctgat caagctgaac aaggcttagc
     3241 gcagcttgtg atgacagtta ttgagctatt gaggcaaatc gttgaacgtc atgccatgag
     3301 gcgggtggag ggtggaacgt tgacggacga acaaattgaa aacttaggaa ttgcactaat
     3361 gaacttagaa gaaaaaatgg acgagttgaa agaggtgttc ggtctggatg cagaagattt
     3421 aaatattgat cttggcccgc taggcagcct gctttaagcg gtcagtagga ggaacagtat
     3481 ggcagtcgaa cataatatgc agtcaagtac gattgtagat gtgctcgaaa agattttgga
     3541 taaaggagtc gttatagcgg gggacatcac cgtaggaatt gcagatgtcg agctattaac
     3601 gataaagatc cgcttgattg tggcttcggt tgataaggca aaagaaatcg gcatggactg
     3661 gtgggaaaat gatccgtatc tcagttcaaa aggagccaat aacaaagcgc tcgaagaaga
     3721 aaataaaatg ctgcatgagc ggttaaaaac gcttgaagaa aaaatagaaa cgaaacgtta
     3781 aaaactgtac gctacttaaa aaatggaggg atttacaatg gcaactgaaa caaaattaga
     3841 taacacacag gcagaaaaca aggaaaataa aaatgcggaa aacggttcaa aagaaaagaa
     3901 cggttcaaaa gcaagcaaaa caacaagcag cgggccaatc aaacgagcgg tagcaggagg
     3961 catcatcggt gcaacgattg gatatgtatc gactcctgaa aatcgaaaaa gtctccttga
     4021 ccgcattgat acagacgaat taaaaagcaa agcatctgat ttaggaacaa aggtaaaaga
     4081 aaaatcaaaa agcagcgtgg ccagcctgaa aacatctgcg ggaagcttgt ttaaaaaaga
     4141 taaagataaa tcaaaagatg atgaagaaaa cgtaaattct tctagtagcg aaacagaaga
     4201 cgataacgtt caagagtacg acgagttaaa agaagaaaat caaactcttc aagatcgctt
     4261 atcacagctt gaagaaaaaa tgaacatgct tgttgagctt agcctcaata aaaatcaaga
     4321 cgaagaagcg gaagatacag attccgacga agaagagaac gatgagaacg atgaaaacga
     4381 tgaaaacgag caggacgatg aaaacgaaga agaaacatct aagccacgta aaaaggataa
     4441 aaaagaagct gaggaagaag aaagtgaaag tgacgaagac agcgaggaag aagaggaaga
     4501 ttctcgctca aacaaaaaaa ataaaaaagt aaaaacagaa gaagaagacg aagatgaaag
     4561 cgaagaagaa aaaaaggaag cgaaaccaaa aaagtcaaca gctaaaaaat caaaaaatac
     4621 aaaagcaaag aaaaacacgg acgaagaaga tgatgaagca acatctcttt ctagtgaaga
     4681 cgatacaaca gcctaagacg taaaggagga aagaaagaca tgagtacagg cccttctttt
     4741 tcaactaaag acaatacgct tgaatacttt gtgaaagctt ctaataaaca cggcttttca
     4801 cttgatattt cattaaatgt aaacggcgct gtgatttccg gtaccatgat ttcagcaaaa
     4861 gaatactttg attacttaag cgaaacgttt gaagaaggca gtgaagtggc tcaggcgcta
     4921 agcgaacaat tctctttagc aagcgaagcg agcgaatcaa acggagaagc agaagcccat
     4981 tttattcatt tgaaaaatac aaagatttac tgtggagaca gtaaatctac tccttctaaa
     5041 ggcaaaatct tttggagagg gaaaatagca gaagtagacg ggtttttctt aggaaagatt
     5101 tctgatgcaa aatcaacgag taaaaagagt tcataaaaaa cggcggggtg attgccccgc
     5161 cgttttttag tgatgtgatg agatgtgcag cttctttttt cgatacatga gcggagtcat
     5221 cttcatttct tttcgaaact gattaataaa atagcttgta ctattaaacc ctacttgata
     5281 ggcgacttcc gtcacatttg cttctgcttg ctgcagtccg gattcgcgaa cgcgtctcga
     5341 gagatccggc tgctaacaaa gcccgaaagg aagctgagtt ggctgctgcc accgctgagc
     5401 aataactagc ataacccctt ggggcctcta aacgggtctt gaggggtttt ttgctgaaag
     5461 gaggaactat atccgactag tagcccgcct aatgagcggg ctttttttta attcccctat
     5521 ttgtttattt ttctaaatac attcaaatat gtatccgctc atgagacaat aaccctgata
     5581 aatgcttcaa taatattgaa aaaggaagag tatgagtatt caacatttcc gtgtcgccct
     5641 tattcccttt tttgcggcat tttgccttcc tgtttttgct cacccagaaa cgctcgtgaa
     5701 agtaaaagac gcagaggacc aattgggggc acgagtggga tacatagaac tggacttgaa
     5761 tagcggtaaa atccttgaga gttttcgccc tgaagagcgt tttccaatga tgagcacttt
     5821 caaagttctg ctatgtggag cagtattatc ccgtgtagat gcggggcaag agcaactcgg
     5881 acgacgaata cactattcgc agaatgactt ggttgaatac tccccagtga cagaaaagca
     5941 ccttacggac ggaatgacgg taagagaatt atgtagtgcc gccataacga tgagtgataa
     6001 cactgcggcg aacttacttc tgacaaccat cggtggaccg aaggaattaa ccgctttttt
     6061 gcacaatatg ggagaccatg taactcgcct tgaccgttgg gaaccagaac tgaatgaagc
     6121 cataccaaac gacgagcgag acaccacaat gcctgcggca atggcaacaa cattacgcaa
     6181 actattaact ggcgaactac ttactctggc ttcacggcaa caattaatag actggcttga
     6241 agcggataaa gttgcaggac cactactgcg ttcggcactt cctgctggct ggtttattgc
     6301 tgataaatct ggggcaggag agcgtggttc acggggtatc attgccgcac ttggaccaga
     6361 tggtaagcct tcccgtatcg tagttatcta cacgacgggt agtcaggcaa ctatggacga
     6421 acgaaataga cagattgctg aaataggggc ttcactgatt aagcattggt aaaccgatac
     6481 aattaaaggc tccttttgga gccttttttt ttggacggac cggtagaaaa gatcaaagga
     6541 tcttcttgag atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg
     6601 ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact
     6661 ggcttcagca gagcgcagat accaaatact gtccttctag tgtagccgta gttaggccac
     6721 cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg
     6781 gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg
     6841 gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga
     6901 acgacctaca ccgaactgag atacctacag cgtgagctat gagaaagcgc cacgcttccc
     6961 gaagggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg
     7021 agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc
     7081 tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaa
//
