LOCUS       sequence-457261-       11571 bp    DNA     circular SYN 15-APR-2025
DEFINITION  Tet-inducible lentiviral vector for 2xHA GPR137 expression.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11571)
  AUTHORS   Pedro MP, Lund K, Kang SWS, Chen T, Stuelten CH, Porat-Shliom N, 
            Iglesias-Bartolome R
  TITLE     GPCR Screening Reveals that the Metabolite Receptor HCAR3 Regulates 
            Epithelial Proliferation, Migration, and Cellular Respiration.
  JOURNAL   J Invest Dermatol. 2024 Jun;144(6):1311-1321.e7. doi: 
            10.1016/j.jid.2023.12.002. Epub 2023 Dec 14.
  PUBMED    38103827
REFERENCE   2  (bases 1 to 11571)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.jid.2023.12.002"; journalName: "J Invest Dermatol"; date:
            "2024-06"; volume: "144"; issue: "6"; pages: "1311-1321.e7"
COMMENT     Sequence Label: pInducer20 2xHA GPR137
FEATURES             Location/Qualifiers
     source          1..11571
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     LTR             1..634
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    681..806
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1303..1536
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1721..1765
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2058..2175
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     protein_bind    join(2230..2248,2249..2271,2272..2290,2291..2313,2314..2332,
                     2333..2355,2356..2374,2375..2399,2400..2418,2419..2441,
                     2442..2460,2461..2483,2484..2502)
                     /label=tetracycline response element
                     /bound_moiety="tetracycline repressor TetR"
                     /note="contains seven copies of the tetracycline operator 
                     tetO"
     promoter        2535..2573
                     /label=minimal CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     2570..2594
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     protein_bind    2703..2727
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             2836..2862
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             2866..2892
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     protein_bind    complement(4143..4167)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             4220..4246
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     promoter        4332..5543
                     /label=UbC promoter
                     /note="human ubiquitin C promoter"
     primer_bind     5380..5399
                     /label=hUBCpro-F
                     /note="Human Ubiquitin C (UbC) promoter, forward primer"
     CDS             join(5602..6219,6220..6225,6226..6261,6262..6264,6265..6300,
                     6301..6303,6304..6339,6340..6348)
                     /codon_start=1
                     /product="improved tetracycline-controlled transactivator"
                     /label=rtTA-Advanced
                     /note="In the Tet-On(R) system, rtTA-Advanced binds to the 
                     Tet-responsive element and stimulates transcription only in
                     the presence of tetracycline or doxycycline."
                     /translation="MSRLDKSKVINGALELLNGVGIEGLTTRKLAQKLGVEQPTLYWHV
                     KNKRALLDALPIEMLDRHHTHFCPLEGESWQDFLRNNAKSYRCALLSHRDGAKVHLGTR
                     PTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEEQEHQVAKEERETPTT
                     DSMPPLLRQAIELFDRQGAEPAFLFGLELIICGLEKQLKCESGGPTDALDDFDLDMLPA
                     DALDDFDLDMLPADALDDFDLDMLPG"
     misc_feature    6401..6974
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(6568..6585)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     6795..6814
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             6990..7784
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     CDS             6990..7784
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(7044..7063)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     7654..7673
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     misc_feature    7961..8549
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(8014..8034)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(8432..8443)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             8624..8857
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     primer_bind     complement(8976..8994)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        9062..9166
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(9167..9235,9236..10027)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(9385..9404)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      10198..10786
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     10687..10706
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     10940..10957
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    11197..11218
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(11233..11238,11239..11256,11257..11263)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    11271..11287
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     11276..11298
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     11295..11311
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     11295..11311
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     11361..11380
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
ORIGIN
        1 tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg atctaccaca
       61 cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac
      121 tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca
      181 ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg
      241 agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag
      301 agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg
      361 ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat
      421 cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga
      481 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct
      541 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc
      601 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag
      661 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg
      721 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga
      781 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg
      841 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg
      901 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct
      961 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat
     1021 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca
     1081 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc
     1141 aagcggccgg ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag
     1201 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc
     1261 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg
     1321 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc
     1381 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc
     1441 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct
     1501 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa
     1561 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca
     1621 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt
     1681 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt
     1741 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta
     1801 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt
     1861 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct
     1921 cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga
     1981 cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcgccgaa ttcacaaatg
     2041 gcagtattca tccacaattt taaaagaaaa ggggggattg gggggtacag tgcaggggaa
     2101 agaatagtag acataatagc aacagacata caaactaaag aattacaaaa acaaattaca
     2161 aaaattcaaa attttcgggt ttattacagg gacagcagag atccagtttg gactaggatc
     2221 ctttaccact ccctatcagt gatagagaaa agtgaaagtc gagtttacca ctccctatca
     2281 gtgatagaga aaagtgaaag tcgagtttac cactccctat cagtgataga gaaaagtgaa
     2341 agtcgagttt accactccct atcagtgata gagaaaagtg aaagtcgagt gtttaccact
     2401 ccctatcagt gatagagaaa agtgaaagtc gagtttacca ctccctatca gtgatagaga
     2461 aaagtgaaag tcgagtttac cactccctat cagtgataga gaaaagtgaa agtcgagctc
     2521 ggtacccggg tcgaggtagg cgtgtacggt gggaggccta tataagcaga gctcgtttag
     2581 tgaaccgtca gatcgcctgg agacgccatc cacgctgttt tgacctccat agaagacacc
     2641 gggaccgatc cagcctccgc ggccccgaac tagtccagtg tggtggaatt ctgcagatat
     2701 caacaagttt gtacaaaaaa gcaggcttta aaggaaccaa ttcagtcgac tggatccggt
     2761 accgctagcg ccgccaccac cttcgatcaa ctcgcgccgc tggtcaataa tggtaccgct
     2821 agcgccgcca ccatgtaccc atacgatgtt cctgactatg cgggctatcc ctatgacgtc
     2881 ccggactatg caggatccgg agatccacct gtcgcaacca tggagtctaa tctgtcaggg
     2941 ttggtccctg cagccgggtt ggtgccagcg ctcccaccag cggtaacact gggattgact
     3001 gctgcttaca ctactcttta cgctctcctt ttcttttccg tatacgcaca attgtggttg
     3061 gtcttgctct acgggcacaa gcgactgagc taccagacag tgttcctcgc cttgtgtctg
     3121 ctgtgggcgg cgttgaggac aactcttttc tctttttatt tcagagatac gccaagagct
     3181 aatcggctcg gccctcttcc attctggctt ctctactgtt gcccagtgtg tttgcaattc
     3241 tttactctga ctttgatgaa tctctacttt gctcaggttg ttttcaaagc gaaagtcaaa
     3301 cgcagacccg agatgtctcg aggattgctt gccgtccgag gtgcttttgt cggggcatct
     3361 ctcctgttcc ttctcgtaaa tgtgctgtgt gctgtgctga gtcaccgacg gcgagcacag
     3421 ccgtgggcgc tgttgcttgt ccgcgtcttg gtgagcgaca gtttgtttgt aatctgtgcc
     3481 ctttcactcg cagcatgcct ctgtctcgtc gctcggcgag caccaagtac aagcatctat
     3541 ttggaagcga aggggaccag tgtgtgccaa gccgctgcta tgggcggcgc catggttttg
     3601 ttgtacgcta gtagggcgtg ctataacttg acagcacttg ccttggcccc gcaaagtagg
     3661 ctggacactt tcgactacga ctggtacaac gtaagcgatc aagcggacct cgtaaacgat
     3721 cttggaaata aagggtatct cgtgttcggt ctgatcctct tcgtctggga actgttgccc
     3781 actaccctgc ttgttggatt tttcagggtc catagaccac cccaggatct ctcaacgtca
     3841 catatactga acggccaagt ttttgcttct agatcctact tcttcgatag agcgggacac
     3901 tgtgaggacg aaggctgctc atgggaacac agtaggggcg agtcaacgag tatgagtggt
     3961 tctctcgggt caggatcatg gtatggtgca atagggaggg aacccggatg gtacggaggt
     4021 agtcaaacga agactacgcc cctgctcttc agtcaggtgc ctgggcccgg gggacaccac
     4081 cacagcctgt actctacccc gcagacctga taagaattcg cggccgcact cgagatatct
     4141 agacccagct ttcttgtaca aagtggttga tatccagcac agtggcggcc gctcgagtct
     4201 agagggcccg cggttcgaat acccatacga cgtcccagac tacgcttagt aatgattaat
     4261 taaactagaa attctaccgg gtaggggagg cgcttttccc aaggcagtct ggagtaaccc
     4321 acccaagatc tggcctccgc gccgggtttt ggcgcctccc gcgggcgccc ccctcctcac
     4381 ggcgagcgct gccacgtcag acgaagggcg cagcgagcgt cctgatcctt ccgcccggac
     4441 gctcaggaca gcggcccgct gctcataaga ctcggcctta gaaccccagt atcagcagaa
     4501 ggacatttta ggacgggact tgggtgactc tagggcactg gttttctttc cagagagcgg
     4561 aacaggcgag gaaaagtagt cccttctcgg cgattctgcg gagggatctc cgtggggcgg
     4621 tgaacgccga tgattatata aggacgcgcc gggtgtggca cagctagttc cgtcgcagcc
     4681 gggatttggg tcgcggttct tgtttgtgga tcgctgtgat cgtcacttgg tgagtagcgg
     4741 gctgctgggc tggccggggc tttcgtggcc gccgggccgc tcggtgggac ggaagcgtgt
     4801 ggagagaccg ccaagggctg tagtctgggt ccgcgagcaa ggttgccctg aactgggggt
     4861 tggggggagc gcagcaaaat ggcggctgtt cccgagtctt gaatggaaga cgcttgtgag
     4921 gcgggctgtg aggtcgttga aacaaggtgg ggggcatggt gggcggcaag aacccaaggt
     4981 cttgaggcct tcgctaatgc gggaaagctc ttattcgggt gagatgggct ggggcaccat
     5041 ctggggaccc tgacgtgaag tttgtcactg actggagaac tcggtttgtc gtctgttgcg
     5101 ggggcggcag ttatggcggt gccgttgggc agtgcacccg tacctttggg agcgcgcgcc
     5161 ctcgtcgtgt cgtgacgtca cccgttctgt tggcttataa tgcagggtgg ggccacctgc
     5221 cggtaggtgt gcggtaggct tttctccgtc gcaggacgca gggttcgggc ctagggtagg
     5281 ctctcctgaa tcgacaggcg ccggacctct ggtgagggga gggataagtg aggcgtcagt
     5341 ttctttggtc ggttttatgt acctatcttc ttaagtagct gaagctccgg ttttgaacta
     5401 tgcgctcggg gttggcgagt gtgttttgtg aagtttttta ggcacctttt gaaatgtaat
     5461 catttgggtc aatatgtaat tttcagtgtt agactagtaa attgtccgct aaattctggc
     5521 cgtttttggc ttttttgtta gacgaagctt ggtaccgagc tcggatctcc accccgtacc
     5581 ggtcctgcag tcgaattcac catgtctaga ctggacaaga gcaaagtcat aaacggagct
     5641 ctggaattac tcaatggtgt cggtatcgaa ggcctgacga caaggaaact cgctcaaaag
     5701 ctgggagttg agcagcctac cctgtactgg cacgtgaaga acaagcgggc cctgctcgat
     5761 gccctgccaa tcgagatgct ggacaggcat catacccact tctgccccct ggaaggcgag
     5821 tcatggcaag actttctgcg gaacaacgcc aagtcatacc gctgtgctct cctctcacat
     5881 cgcgacgggg ctaaagtgca tctcggcacc cgcccaacag agaaacagta cgaaaccctg
     5941 gaaaatcagc tcgcgttcct gtgtcagcaa ggcttctccc tggagaacgc actgtacgct
     6001 ctgtccgccg tgggccactt tacactgggc tgcgtattgg aggaacagga gcatcaagta
     6061 gcaaaagagg aaagagagac acctaccacc gattctatgc ccccacttct gagacaagca
     6121 attgagctgt tcgaccggca gggagccgaa cctgccttcc ttttcggcct ggaactaatc
     6181 atatgtggcc tggagaaaca gctaaagtgc gaaagcggcg ggccgaccga cgcccttgac
     6241 gattttgact tagacatgct cccagccgat gcccttgacg actttgacct tgatatgctg
     6301 cctgctgacg ctcttgacga ttttgacctt gacatgctcc ccgggtaact aagtaaggat
     6361 ccgcggccgc actagaggaa ttccgcccct ctcccccccc cccctctccc tccccccccc
     6421 ctaacgttac tggccgaagc cgcttggaat aaggccggtg tgcgtttgtc tatatgttat
     6481 tttccaccat attgccgtct tttggcaatg tgagggcccg gaaacctggc cctgtcttct
     6541 tgacgagcat tcctaggggt ctttcccctc tcgccaaagg aatgcaaggt ctgttgaatg
     6601 tcgtgaagga agcagttcct ctggaagctt cttgaagaca aacaacgtct gtagcgaccc
     6661 tttgcaggca gcggaacccc ccacctggcg acaggtgcct ctgcggccaa aagccacgtg
     6721 tataagatac acctgcaaag gcggcacaac cccagtgcca cgttgtgagt tggatagttg
     6781 tggaaagagt caaatggctc tcctcaagcg tattcaacaa ggggctgaag gatgcccaga
     6841 aggtacccca ttgtatggga tctgatctgg ggcctcggtg cacatgcttt acatgtgttt
     6901 agtcgaggtt aaaaaaacgt ctaggccccc cgaaccacgg ggacgtggtt ttcctttgaa
     6961 aaacacgatg ataagcttac cggtccacca tgattgaaca agatggattg cacgcaggtt
     7021 ctccggccgc ttgggtggag aggctattcg gctatgactg ggcacaacag acaatcggct
     7081 gctctgatgc cgccgtgttc cggctgtcag cgcaggggcg cccggttctt tttgtcaaga
     7141 ccgacctgtc cggtgccctg aatgaactgc aagacgaggc agcgcggcta tcgtggctgg
     7201 ccacgacggg cgttccttgc gcagctgtgc tcgacgttgt cactgaagcg ggaagggact
     7261 ggctgctatt gggcgaagtg ccggggcagg atctcctgtc atctcacctt gctcctgccg
     7321 agaaagtatc catcatggct gatgcaatgc ggcggctgca tacgcttgat ccggctacct
     7381 gcccattcga ccaccaagcg aaacatcgca tcgagcgagc acgtactcgg atggaagccg
     7441 gtcttgtcga tcaggatgat ctggacgaag agcatcaggg gctcgcgcca gccgaactgt
     7501 tcgccaggct caaggcgagc atgcccgacg gcgaggatct cgtcgtgacc catggcgatg
     7561 cctgcttgcc gaatatcatg gtggaaaatg gccgcttttc tggattcatc gactgtggcc
     7621 ggctgggtgt ggcggaccgc tatcaggaca tagcgttggc tacccgtgat attgctgaag
     7681 agcttggcgg cgaatgggct gaccgcttcc tcgtgcttta cggtatcgcc gctcccgatt
     7741 cgcagcgcat cgccttctat cgccttcttg acgagttctt ctgatgtaca agtaaagcgg
     7801 ccgcgactct agatcataat cagccatacc acatttgtag aggttttact tgctttaaaa
     7861 aacctcccac acctccccct gaacctgaaa cataaaatga atgcaattgt tgttgtttag
     7921 tccctcccaa ttcgatatca agcttatcgc gatagatcct aatcaacctc tggattacaa
     7981 aatttgtgaa agattgactg gtattcttaa ctatgttgct ccttttacgc tatgtggata
     8041 cgctgcttta atgcctttgt atcatgctat tgcttcccgt atggctttca ttttctcctc
     8101 cttgtataaa tcctggttgc tgtctcttta tgaggagttg tggcccgttg tcaggcaacg
     8161 tggcgtggtg tgcactgtgt ttgctgacgc aacccccact ggttggggca ttgccaccac
     8221 ctgtcagctc ctttccggga ctttcgcttt ccccctccct attgccacgg cggaactcat
     8281 cgccgcctgc cttgcccgct gctggacagg ggctcggctg ttgggcactg acaattccgt
     8341 ggtgttgtcg gggaaatcat cgtcctttcc ttggctgctc gcctgtgttg ccacctggat
     8401 tctgcgcggg acgtccttct gctacgtccc ttcggccctc aatccagcgg accttccttc
     8461 ccgcggcctg ctgccggctc tgcggcctct tccgcgtctt cgccttcgcc ctcagacgag
     8521 tcggatctcc ctttgggccg cctccccgcc tgagatcctt taagaccaat gacttacaag
     8581 gcagctgtag atcttagcca ctttttaaaa gaaaaggggg gactggaagg gctaattcac
     8641 tcccaacgaa gacaagatct gctttttgct tgtactgggt ctctctggtt agaccagatc
     8701 tgagcctggg agctctctgg ctaactaggg aacccactgc ttaagcctca ataaagcttg
     8761 ccttgagtgc ttcaagtagt gtgtgcccgt ctgttgtgtg actctggtaa ctagagatcc
     8821 ctcagaccct tttagtcagt gtggaaaatc tctagcagta gtagttcatg tcatcttatt
     8881 attcagtatt tataacttgc aaagaaatga atatcagaga gtgagaggcc cgggttaatt
     8941 aaggaaaggg ctagatcatt cttgaagacg aaagggcctc gtgatacgcc tatttttata
     9001 ggttaatgtc atgataataa tggtttctta gacgtcaggt ggcacttttc ggggaaatgt
     9061 gcgcggaacc cctatttgtt tatttttcta aatacattca aatatgtatc cgctcatgag
     9121 acaataaccc tgataaatgc ttcaataata ttgaaaaagg aagagtatga gtattcaaca
     9181 tttccgtgtc gcccttattc ccttttttgc ggcattttgc cttcctgttt ttgctcaccc
     9241 agaaacgctg gtgaaagtaa aagatgctga agatcagttg ggtgcacgag tgggttacat
     9301 cgaactggat ctcaacagcg gtaagatcct tgagagtttt cgccccgaag aacgttttcc
     9361 aatgatgagc acttttaaag ttctgctatg tggcgcggta ttatcccgtg ttgacgccgg
     9421 gcaagagcaa ctcggtcgcc gcatacacta ttctcagaat gacttggttg agtactcacc
     9481 agtcacagaa aagcatctta cggatggcat gacagtaaga gaattatgca gtgctgccat
     9541 aaccatgagt gataacactg cggccaactt acttctgaca acgatcggag gaccgaagga
     9601 gctaaccgct tttttgcaca acatggggga tcatgtaact cgccttgatc gttgggaacc
     9661 ggagctgaat gaagccatac caaacgacga gcgtgacacc acgatgcctg tagcaatggc
     9721 aacaacgttg cgcaaactat taactggcga actacttact ctagcttccc ggcaacaatt
     9781 aatagactgg atggaggcgg ataaagttgc aggaccactt ctgcgctcgg cccttccggc
     9841 tggctggttt attgctgata aatctggagc cggtgagcgt gggtctcgcg gtatcattgc
     9901 agcactgggg ccagatggta agccctcccg tatcgtagtt atctacacga cggggagtca
     9961 ggcaactatg gatgaacgaa atagacagat cgctgagata ggtgcctcac tgattaagca
    10021 ttggtaactg tcagaccaag tttactcata tatactttag attgatttaa aacttcattt
    10081 ttaatttaaa aggatctagg tgaagatcct ttttgataat ctcatgacca aaatccctta
    10141 acgtgagttt tcgttccact gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg
    10201 agatcctttt tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac cgctaccagc
    10261 ggtggtttgt ttgccggatc aagagctacc aactcttttt ccgaaggtaa ctggcttcag
    10321 cagagcgcag ataccaaata ctgttcttct agtgtagccg tagttaggcc accacttcaa
    10381 gaactctgta gcaccgccta catacctcgc tctgctaatc ctgttaccag tggctgctgc
    10441 cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac cggataaggc
    10501 gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc agcttggagc gaacgaccta
    10561 caccgaactg agatacctac agcgtgagct atgagaaagc gccacgcttc ccgaagggag
    10621 aaaggcggac aggtatccgg taagcggcag ggtcggaaca ggagagcgca cgagggagct
    10681 tccaggggga aacgcctggt atctttatag tcctgtcggg tttcgccacc tctgacttga
    10741 gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc
    10801 ggccttttta cggttcctgg ccttttgctg gccttttgct cacatgttct ttcctgcgtt
    10861 atcccctgat tctgtggata accgtattac cgcctttgag tgagctgata ccgctcgccg
    10921 cagccgaacg accgagcgca gcgagtcagt gagcgaggaa gcggaagagc gcccaatacg
    10981 caaaccgcct ctccccgcgc gttggccgat tcattaatgc agcaagctca tggctgacta
    11041 atttttttta tttatgcaga ggccgaggcc gcctcggcct ctgagctatt ccagaagtag
    11101 tgaggaggct tttttggagg cctaggcttt tgcaaaaagc tccccgtggc acgacaggtt
    11161 tcccgactgg aaagcgggca gtgagcgcaa cgcaattaat gtgagttagc tcactcatta
    11221 ggcaccccag gctttacact ttatgcttcc ggctcgtatg ttgtgtggaa ttgtgagcgg
    11281 ataacaattt cacacaggaa acagctatga catgattacg aatttcacaa ataaagcatt
    11341 tttttcactg cattctagtt gtggtttgtc caaactcatc aatgtatctt atcatgtctg
    11401 gatcaactgg ataactcaag ctaaccaaaa tcatcccaaa cttcccaccc cataccctat
    11461 taccactgcc aattacctgt ggtttcattt actctaaacc tgtgattcct ctgaattatt
    11521 ttcattttaa agaaattgta tttgttaaat atgtactaca aacttagtag t
//
