LOCUS       sequence-457326-        9179 bp    DNA     circular SYN 15-APR-2025
DEFINITION  Encoding Merlin fused to mCherry..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9179)
  TITLE     Collective cell migration is coordinated by front-biased activation 
            of the feed-forward loop consisting of Ras, Rab5, and Rac1.
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 9179)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pPBneo-UbC-mCherry-Merlin
FEATURES             Location/Qualifiers
     source          1..9179
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      complement(30..458)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(140..161)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     352..371
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     585..607
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     599..616
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     600..616
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     626..645
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        626..644
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     repeat_region   674..708
                     /label=piggyBac left (5') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
     promoter        1004..2215
                     /label=UbC promoter
                     /note="human ubiquitin C promoter"
     primer_bind     2052..2071
                     /label=hUBCpro-F
                     /note="Human Ubiquitin C (UbC) promoter, forward primer"
     CDS             2233..2937
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELY"
     primer_bind     complement(2378..2396)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     2646..2665
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     misc_feature    4877..5426
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(5021..5038)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     5248..5267
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             5429..6223
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(5483..5502)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     6093..6112
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     complement(6263..6280)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    6269..6493
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     complement(6540..6556)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    6564..6580
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(6588..6594,6595..6612,6613..6618))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6633..6654
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     repeat_region   6888..6950
                     /label=piggyBac right (3') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
     primer_bind     complement(6991..7011)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(6991..7009)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(7030..7046)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    7054..7070
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(7078..7084,7085..7102,7103..7108))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7123..7144
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(7261..7278)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7432..8020)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7512..7531)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8191..8982,8983..9051))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8814..8833
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9052..9156)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 ctaaattgta agcgttaata ttttgttaaa attcgcgtta aatttttgtt aaatcagctc
       61 attttttaac caataggccg aaatcggcaa aatcccttat aaatcaaaag aatagaccga
      121 gatagggttg agtgttgttc cagtttggaa caagagtcca ctattaaaga acgtggactc
      181 caacgtcaaa gggcgaaaaa ccgtctatca gggcgatggc ccactacgtg aaccatcacc
      241 ctaatcaagt tttttggggt cgaggtgccg taaagcacta aatcggaacc ctaaagggag
      301 cccccgattt agagcttgac ggggaaagcc ggcgaacgtg gcgagaaagg aagggaagaa
      361 agcgaaagga gcgggcgcta gggcgctggc aagtgtagcg gtcacgctgc gcgtaaccac
      421 cacacccgcc gcgcttaatg cgccgctaca gggcgcgtcc cattcgccat tcaggctgcg
      481 caactgttgg gaagggcgat cggtgcgggc ctcttcgcta ttacgccagc tggcgaaagg
      541 gggatgtgct gcaaggcgat taagttgggt aacgccaggg ttttcccagt cacgacgttg
      601 taaaacgacg gccagtgagc gcgcgtaata cgactcacta tagggcgaat tggggcgcgc
      661 cattctagat taaccctaga aagatagtct gcgtaaaatt gacgcatgca ttcttgaaat
      721 attgctctct ctttctaaat agcgcgaatc cgtcgctgtg catttaggac atctcagtcg
      781 ccgcttggag ctcccgtgag gcgtgcttgt caatgcggta agtgtcactg attttgaact
      841 ataacgaccg cgtgagtcaa aatgacgcat gattatcttt tacgtgactt ttaagattta
      901 actcatacga taattatatt gttatttcat gttctactta cgtgataact tattatatat
      961 atattttctt gttatagata tcaactagaa tgctagcctc gagggcctcc gcgccgggtt
     1021 ttggcgcctc ccgcgggcgc ccccctcctc acggcgagcg ctgccacgtc agacgaaggg
     1081 cgcagcgagc gtcctgatcc ttccgcccgg acgctcagga cagcggcccg ctgctcataa
     1141 gactcggcct tagaacccca gtatcagcag aaggacattt taggacggga cttgggtgac
     1201 tctagggcac tggttttctt tccagagagc ggaacaggcg aggaaaagta gtcccttctc
     1261 ggcgattctg cggagggatc tccgtggggc ggtgaacgcc gatgattata taaggacgcg
     1321 ccgggtgtgg cacagctagt tccgtcgcag ccgggatttg ggtcgcagtt cttgtttgtg
     1381 gatcgctgtg atcgtcactt ggtgagtagc gggctgctgg gctggccggg gctttcgtgg
     1441 ccgccgggcc gctcggtggg acggaggcgt gtggagagac cgccaagggc tgtagtctgg
     1501 gtccgcgagc aaggttgccc tgaactgggg gttgggggga gcgcagcaaa atggcggctg
     1561 ttcccgagtc ttgaatggaa gacgcttgtg aggcgggctg tgaggtcgtt gaaacaaggt
     1621 ggggggcatg gtgggcggca agaacccaag gtcttgaggc cttcgctaat gcgggaaagc
     1681 tcttattcgg gtgagatggg ctggggcacc atctggggac cctgacgtga agtttgtcac
     1741 tgactggaga actcggtttg tcgtctgttg cgggggcggc agttatggcg gtgccgttgg
     1801 gcagtgcacc cgtacctttg ggagcgcgcg ccctcgtcgt gtcgtgacgt cacccgttct
     1861 gttggcttat aatgcagggt ggggccacct gccggtaggt gtgcggtagg cttttctccg
     1921 tcgcaggacg cagggttcgg gcctagggta ggctctcctg aatcgacagg cgccggacct
     1981 ctggtgaggg gagggataag tgaggcgtca gtttctctgg tcggttttat gtacctatct
     2041 tcttaagtag ctgaagctcc ggttttgaac tatgcgctcg gggttggcga gtgtgttttg
     2101 tgaagttttt taggcacctt ttgaaatgta atcatttggg tcaatatgta attttcagtg
     2161 ttagactagt aaattgtccg ctaaattctg gccgtttttg gcttttttgt tagacggatc
     2221 catgaattca ccatggtgag caagggcgag gaggataaca tggccatcat caaggagttc
     2281 atgcgcttca aggtgcacat ggagggctcc gtgaacggcc acgagttcga gatcgagggc
     2341 gagggcgagg gccgccccta cgagggcacc cagaccgcca agctgaaggt gaccaagggt
     2401 ggccccctgc ccttcgcctg ggacatcctg tcccctcagt tcatgtacgg ctccaaggcc
     2461 tacgtgaagc accccgccga catccccgac tacttgaagc tgtccttccc cgagggcttc
     2521 aagtgggagc gcgtgatgaa cttcgaggac ggcggcgtgg tgaccgtgac ccaggactcc
     2581 tccctgcagg acggcgagtt catctacaag gtgaagctgc gcggcaccaa cttcccctcc
     2641 gacggccccg taatgcagaa gaagaccatg ggctgggagg cctcctccga gcggatgtac
     2701 cccgaggacg gcgccctgaa gggcgagatc aagcagaggc tgaagctgaa ggacggcggc
     2761 cactacgacg ctgaggtcaa gaccacctac aaggccaaga agcccgtgca gctgcccggc
     2821 gcctacaacg tcaacatcaa gttggacatc acctcccaca acgaggacta caccatcgtg
     2881 gaacagtacg aacgcgccga gggccgccac tccaccggcg gcatggacga gctgtacctc
     2941 gacatggccg gggccatcgc ttcccgcatg agcttcagct ctctcaagag gaagcaaccc
     3001 aagacgttca ccgtgaggat cgtcaccatg gacgccgaga tggagttcaa ttgcgagatg
     3061 aagtggaaag ggaaggacct ctttgatttg gtgtgccgga ctctggggct ccgagaaacc
     3121 tggttctttg gactgcagta cacaatcaag gacacagtgg cctggctcaa aatggacaag
     3181 aaggtactgg atcatgatgt ttcaaaggaa gaaccagtca cctttcactt cttggccaaa
     3241 ttttatcctg agaatgctga agaggagctg gttcaggaga tcacacaaca tttattcttc
     3301 ttacaggtaa agaagcagat tttagatgaa aagatctact gcccccctga ggcttctgtg
     3361 ctacttgcga gctatgcagt acaagccaag tatggtgact acgaccccag tgttcacaag
     3421 cggggatttt tggcccaaga ggaattgctt ccaaaaaggg taataaatct gtatcagatg
     3481 actccggaaa tgtgggagga gagaattact gcttggtacg cagagcaccg aggccgagcc
     3541 agggatgaag ctgaaatgga atatctgaag atagctcagg acctggagat gtacggtgtg
     3601 aactactttg caatccggaa taaaaagggc acagagctgc tgcttggagt ggatgccctg
     3661 gggcttcaca tttatgaccc tgagaacaga ctgaccccca agatctcctt cccgtggaat
     3721 gaaatccgaa acatctcgta cagtgacaag gagtttacta ttaaaccact ggataagaaa
     3781 attgatgtct tcaagtttaa ctcctcaaag cttcgtgtta ataagctgat tctccagcta
     3841 tgtatcggga accatgatct atttatgagg agaaggaaag ccgattcttt ggaagttcag
     3901 cagatgaaag cccaggccag ggaggagaag gctagaaagc agatggagcg gcagcgcctc
     3961 gctcgagaga agcagatgag ggaggaggct gaacgcacga gggatgagtt ggagaggagg
     4021 ctgctgcaga tgaaagaaga agcaacaatg gccaacgaag cactgatgcg gtctgaggag
     4081 acagctgacc tgttggctga aaaggcccag atcaccgagg aggaggcaaa acttctggcc
     4141 cagaaggccg cagaggctga gcaggaaatg cagcgcatca aggccacagc gattcgcacg
     4201 gaggaggaga agcgcctgat ggagcagaag gtgctggaag ccgaggtgct ggcactgaag
     4261 atggctgagg agtcagagag gagggccaaa gaggcagatc agctgaagca ggacctgcag
     4321 gaagcacgcg aggcggagcg aagagccaag cagaagctcc tggagattgc caccaagccc
     4381 acgtacccgc ccatgaaccc aattccagca ccgttgcctc ctgacatacc aagcttcaac
     4441 ctcattggtg acagcctgtc tttcgacttc aaagatactg acatgaagcg gctttccatg
     4501 gagatagaga aagaaaaagt ggaatacatg gaaaagagca agcatctgca ggagcagctc
     4561 aatgaactca agacagaaat cgaggccttg aaactgaaag agagggagac agctctggat
     4621 attctgcaca atgagaactc cgacaggggt ggcagcagca agcacaatac cattaaaaag
     4681 cctcaagccc aaggcagaag acctatctgc atttgagccc tcaaactcac cttgcagagc
     4741 gccaagtccc gagtggcctt ctttgaagag ctctaggcgg ccgctctaga gtcgacgggc
     4801 cgcggtaaca attgttaact aacttaagct agcaacggtt tccctctagc gggatcaatt
     4861 ccgccccccc cccctaacgt tactggccga agccgcttgg aataaggccg gtgtgcgttt
     4921 gtctatatgt tattttccac catattgccg tcttttggca atgtgagggc ccggaaacct
     4981 ggccctgtct tcttgacgag cattcctagg ggtctttccc ctctcgccaa aggaatgcaa
     5041 ggtctgttga atgtcgtgaa ggaagcagtt cctctggaag cttcttgaag acaaacaacg
     5101 tctgtagcga ccctttgcag gcagcggaac cccccacctg gcgacaggtg cctctgcggc
     5161 caaaagccac gtgtataaga tacacctgca aaggcggcac aaccccagtg ccacgttgtg
     5221 agttggatag ttgtggaaag agtcaaatgg ctctcctcaa gcgtattcaa caaggggctg
     5281 aaggatgccc agaaggtacc ccattgtatg ggatctgatc tggggcctcg gtgcacatgc
     5341 tttacatgtg tttagtcgag gttaaaaaaa cgtctaggcc ccccgaacca cggggacgtg
     5401 gttttccttt gaaaaacacg ataataccat gattgaacaa gatggattgc atgcaggttc
     5461 gccggccgct tgggtggaga ggctattcgg ctatgactgg gcacaacaga caatcggctg
     5521 ctctgatgcc gccgtgttcc ggctgtcagc gcaggggcgc ccggttcttt ttgtcaagac
     5581 cgacctgtcc ggtgccctga atgaactgca ggacgaggca gcgcggctat cgtggctggc
     5641 cacgacgggc gttccttgcg cagctgtgct cgacgttgtc actgaagcgg gaagggactg
     5701 gctgctattg ggcgaagtgc cggggcagga tctcctgtca tctcaccttg ctcctgccga
     5761 gaaagtatcc atcatggctg atgcaatgcg gcggctgcat acgcttgatc cggctacctg
     5821 cccattcgac caccaagcga aacatcgcat cgagcgagca cgtactcgga tggaagccgg
     5881 tcttgtcgat caggatgatc tggacgaaga gcatcagggg ctcgcgccag ccgaactgtt
     5941 cgccaggctc aaggcgcgca tgcccgacgg cgaggatctc gtcgtgaccc atggcgatgc
     6001 ctgcttgccg aatatcatgg tggaaaatgg ccgcttttct ggattcatcg actgtggccg
     6061 gctgggtgtg gcggaccgct atcaggacat agcgttggct acccgtgata ttgctgaaga
     6121 gcttggcggc gaatgggctg accgcttcct cgtgctttac ggtatcgccg ctcccgattc
     6181 gcagcgcatc gccttctatc gccttcttga cgagttcttc tgaaagtcga gatgcatcga
     6241 tgatctagag ctcgctgatc agcctcgact gtgccttcta gttgccagcc atctgttgtt
     6301 tgcccctccc ccgtgccttc cttgaccctg gaaggtgcca ctcccactgt cctttcctaa
     6361 taaaatgagg aaattgcatc gcattgtctg agtaggtgtc attctattct ggggggtggg
     6421 gtggggcagg acagcaaggg ggaggattgg gaagacaata gcaggcatgc tggggatgcg
     6481 gtgggctcta tggcttctga ggcggaaaga acctgcagcc caagcttggc gtaatcatgg
     6541 tcatagctgt ttcctgtgtg aaattgttat ccgctcacaa ttccacacaa catacgagcc
     6601 ggaagcataa agtgtaaagc ctggggtgcc taatgagtga gctaactcac attaattgcg
     6661 ttgcgctcac tgcccgcttt ccagtcggga aacctgtcgt gccagcggat cttaaaagtt
     6721 ttgttacttt atagaagaaa ttttgagttt ttgttttttt ttaataaata aataaacata
     6781 aataaattgt ttgttgaatt tattattagt atgtaagtgt aaatataata aaacttaata
     6841 tctattcaaa ttaataaata aacctcgata tacagaccga taaaacacat gcgtcaattt
     6901 tacgcatgat tatctttaac gtacgtcaca atatgattat ctttctaggg ttaatctagt
     6961 atacgcgttt aattaatcca gcttttgttc cctttagtga gggttaattg cgcgcttggc
     7021 gtaatcatgg tcatagctgt ttcctgtgtg aaattgttat ccgctcacaa ttccacacaa
     7081 catacgagcc ggaagcataa agtgtaaagc ctggggtgcc taatgagtga gctaactcac
     7141 attaattgcg ttgcgctcac tgcccgcttt ccagtcggga aacctgtcgt gccagctgca
     7201 ttaatgaatc ggccaacgcg cggggagagg cggtttgcgt attgggcgct cttccgcttc
     7261 ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat cagctcactc
     7321 aaaggcggta atacggttat ccacagaatc aggggataac gcaggaaaga acatgtgagc
     7381 aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag
     7441 gctccgcccc cctgacgagc atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc
     7501 gacaggacta taaagatacc aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt
     7561 tccgaccctg ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct
     7621 ttctcatagc tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg
     7681 ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc ttatccggta actatcgtct
     7741 tgagtccaac ccggtaagac acgacttatc gccactggca gcagccactg gtaacaggat
     7801 tagcagagcg aggtatgtag gcggtgctac agagttcttg aagtggtggc ctaactacgg
     7861 ctacactaga agaacagtat ttggtatctg cgctctgctg aagccagtta ccttcggaaa
     7921 aagagttggt agctcttgat ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt
     7981 ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc
     8041 tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg tcatgagatt
     8101 atcaaaaagg atcttcacct agatcctttt aaattaaaaa tgaagtttta aatcaatcta
     8161 aagtatatat gagtaaactt ggtctgacag ttaccaatgc ttaatcagtg aggcacctat
     8221 ctcagcgatc tgtctatttc gttcatccat agttgcctga ctccccgtcg tgtagataac
     8281 tacgatacgg gagggcttac catctggccc cagtgctgca atgataccgc gagacccacg
     8341 ctcaccggct ccagatttat cagcaataaa ccagccagcc ggaagggccg agcgcagaag
     8401 tggtcctgca actttatccg cctccatcca gtctattaat tgttgccggg aagctagagt
     8461 aagtagttcg ccagttaata gtttgcgcaa cgttgttgcc attgctacag gcatcgtggt
     8521 gtcacgctcg tcgtttggta tggcttcatt cagctccggt tcccaacgat caaggcgagt
     8581 tacatgatcc cccatgttgt gcaaaaaagc ggttagctcc ttcggtcctc cgatcgttgt
     8641 cagaagtaag ttggccgcag tgttatcact catggttatg gcagcactgc ataattctct
     8701 tactgtcatg ccatccgtaa gatgcttttc tgtgactggt gagtactcaa ccaagtcatt
     8761 ctgagaatag tgtatgcggc gaccgagttg ctcttgcccg gcgtcaatac gggataatac
     8821 cgcgccacat agcagaactt taaaagtgct catcattgga aaacgttctt cggggcgaaa
     8881 actctcaagg atcttaccgc tgttgagatc cagttcgatg taacccactc gtgcacccaa
     8941 ctgatcttca gcatctttta ctttcaccag cgtttctggg tgagcaaaaa caggaaggca
     9001 aaatgccgca aaaaagggaa taagggcgac acggaaatgt tgaatactca tactcttcct
     9061 ttttcaatat tattgaagca tttatcaggg ttattgtctc atgagcggat acatatttga
     9121 atgtatttag aaaaataaac aaataggggt tccgcgcaca tttccccgaa aagtgccac
//
