LOCUS       sequence-457426-        6324 bp    DNA     circular SYN 14-APR-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6324)
  AUTHORS   Sena-Esteves M, Tebbets JC, Steffens S, Crombleholme T, Flake AW
  TITLE     Optimized large-scale production of high titer lentivirus vector 
            pseudotypes.
  JOURNAL   J Virol Methods. 2004 Dec 15. 122(2):131-9.
  PUBMED    15542136
REFERENCE   2  (bases 1 to 6324)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 14, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Virol 
            Methods. 2004 Dec 15. 122(2):131-9."
COMMENT     Sequence Label: pHCMV-LCMV-Arm53b
FEATURES             Location/Qualifiers
     source          1..6324
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     intron          27..599
                     /label=beta-globin intron
                     /note="intron from rabbit beta-globin gene"
     CDS             complement(1047..1058)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(2264..2283)
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     polyA_signal    2329..2384
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(2383..2402)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     primer_bind     complement(2742..2758)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2742..2758)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(2755..2777)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    2766..2782
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(2790..2796,2797..2814,2815..2820))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    2835..2856
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(2973..2990)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(3144..3732)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(3224..3243)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(3903..4694,4695..4763))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     4526..4545
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(4764..4868)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(4894..5349)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5031..5052)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     5243..5262
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     5476..5498
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     5490..5507
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     5491..5507
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     5517..5536
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        5517..5535
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     enhancer        5656..6035
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        6036..6239
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     6190..6210
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     6236..6260
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
ORIGIN
        1 ggtcgaccga tcctgagaac ttcagggtga gtttggggac ccttgattgt tctttctttt
       61 tcgctattgt aaaattcatg ttatatggag ggggcaaagt tttcagggtg ttgtttagaa
      121 tgggaagatg tcccttgtat caccatggac cctcatgata attttgtttc tttcactttc
      181 tactctgttg acaaccattg tctcctctta ttttcttttc attttctgta actttttcgt
      241 taaactttag cttgcatttg taacgaattt ttaaattcac ttttgtttat ttgtcagatt
      301 gtaagtactt tctctaatca cttttttttc aaggcaatca gggtatatta tattgtactt
      361 cagcacagtt ttagagaaca attgttataa ttaaatgata aggtagaata tttctgcata
      421 taaattctgg ctggcgtgga aatattctta ttggtagaaa caactacacc ctggtcatca
      481 tcctgccttt ctctttatgg ttacaatgat atacactgtt tgagatgagg ataaaatact
      541 ctgagtccaa accgggcccc tctgctaacc atgttcatgc cttcttctct ttcctacagc
      601 tcctgggcaa cgtgctggtt gttgtgctgt ctcatcattt tggcaaagaa ttcctcgacg
      661 gatccgccat gggtcagatt gtggcaatgt ttgaggctct gcctcacatc atcgatgagg
      721 tgatcaacat tgtcattatt gtgcttatcg tgatcacggg tatcaaggct gtctacaatt
      781 ttgccacctg tgggatattc gcattgatca gtttcctact tctggctggc aggtcctgtg
      841 gcatgtacgg tcttaaggga cccgacattt acaaaggagt ttaccaattt aagtcagtgg
      901 agtttgatat gtcacatctg aacctgacca tgcccaacgc atgttcagcc aacaactccc
      961 accattacat cagtatgggg acttctggac tagaattgac cttcaccaat gattccatca
     1021 tcagtcacaa cttttgcaat ctgacctctg ccttcaataa aaagaccttt gaccacacac
     1081 tcatgagtat agtttcgagc ctacacctca gtatcagagg gaactccaac tataaggcag
     1141 tatcctgcga cttcaacaat ggcataacca tccaatacaa cttgacattc tcaaatgcac
     1201 aaagtgctca gagccagtgt agaaccttca gaggtagagt cctagatatg tttagaactg
     1261 ccttcggggg gaaatacatg aggagtggct ggggctggac aggctcagat ggcaagacca
     1321 cctggtgtag ccagacgagt taccaatacc tgattataca aaatagaacc tgggaaaacc
     1381 actgcacata tgcaggtcct tttgggatgt ccaggattct cctttcccaa gagaagacta
     1441 agttcttcac taggagacta gcgggcacat tcacctggac tttgtcagac tcttcagggg
     1501 tggagaatcc aggtggttat tgcctgacca aatggatgat tcttgctgca gagcttaagt
     1561 gtttcgggaa cacagcagtt gcgaaatgca atgtaaatca tgatgaagaa ttctgtgaca
     1621 tgctgcgact aattgactac aacaaggctg ctttgagtaa gttcaaagag gacgtagaat
     1681 ctgccttgca cttattcaaa acaacagtga attctttgat ttcagatcaa ctactgatga
     1741 ggaaccactt gagagatctg atgggggtgc catattgcaa ttactcaaag ttttggtacc
     1801 tagaacatgc aaagaccggc gaaactagtg tccccaagtg ctggcttgtc accaatggtt
     1861 cttacttaaa tgagacccac ttcagtgatc aaatcgaaca ggaagccgat aacatgatta
     1921 cagagatgtt gaggaaggat tacataaaga ggcaggggag taccccccta gcattgatgg
     1981 accttctgat gttttccaca tctgcatatc tagtcagcat cttcctgcac cttgtcaaaa
     2041 taccaacaca caggcacata aaaggtggct catgtccaaa gccacaccga ttaaccaaca
     2101 aaggaatttg tagttgtggt gcatttaagg tgcctggtgt aaaaaccgtc tggaaaagac
     2161 gctgataagg atccgtcgag gaattcactc ctcaggtgca ggctgcctat cagaaggtgg
     2221 tggctggtgt ggccaatgcc ctggctcaca aataccactg agatcttttt ccctctgcca
     2281 aaaattatgg ggacatcatg aagccccttg agcatctgac ttctggctaa taaaggaaat
     2341 ttattttcat tgcaatagtg tgttggaatt ttttgtgtct ctcactcgga aggacatatg
     2401 ggagggcaaa tcatttaaaa catcagaatg agtatttggt ttagagtttg gcaacatatg
     2461 cccatatgct ggctgccatg aacaaaggtt ggctataaag aggtcatcag tatatgaaac
     2521 agccccctgc tgtccattcc ttattccata gaaaagcctt gacttgaggt tagatttttt
     2581 ttatattttg ttttgtgtta tttttttctt taacatccct aaaattttcc ttacatgttt
     2641 tactagccag atttttcctc ctctcctgac tactcccagt catagctgtc cctcttctct
     2701 tatggagatc cctcgacgga tcggccgcaa ttcgtaatca tgtcatagct gtttcctgtg
     2761 tgaaattgtt atccgctcac aattccacac aacatacgag ccggaagcat aaagtgtaaa
     2821 gcctggggtg cctaatgagt gagctaactc acattaattg cgttgcgctc actgcccgct
     2881 ttccagtcgg gaaacctgtc gtgccagctg cattaatgaa tcggccaacg cgcggggaga
     2941 ggcggtttgc gtattgggcg ctcttccgct tcctcgctca ctgactcgct gcgctcggtc
     3001 gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg taatacggtt atccacagaa
     3061 tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt
     3121 aaaaaggccg cgttgctggc gtttttccat aggctccgcc cccctgacga gcatcacaaa
     3181 aatcgacgct caagtcagag gtggcgaaac ccgacaggac tataaagata ccaggcgttt
     3241 ccccctggaa gctccctcgt gcgctctcct gttccgaccc tgccgcttac cggatacctg
     3301 tccgcctttc tcccttcggg aagcgtggcg ctttctcata gctcacgctg taggtatctc
     3361 agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc
     3421 gaccgctgcg ccttatccgg taactatcgt cttgagtcca acccggtaag acacgactta
     3481 tcgccactgg cagcagccac tggtaacagg attagcagag cgaggtatgt aggcggtgct
     3541 acagagttct tgaagtggtg gcctaactac ggctacacta gaagaacagt atttggtatc
     3601 tgcgctctgc tgaagccagt taccttcgga aaaagagttg gtagctcttg atccggcaaa
     3661 caaaccaccg ctggtagcgg tggttttttt gtttgcaagc agcagattac gcgcagaaaa
     3721 aaaggatctc aagaagatcc tttgatcttt tctacggggt ctgacgctca gtggaacgaa
     3781 aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt
     3841 ttaaattaaa aatgaagttt taaatcaatc taaagtatat atgagtaaac ttggtctgac
     3901 agttaccaat gcttaatcag tgaggcacct atctcagcga tctgtctatt tcgttcatcc
     3961 atagttgcct gactccccgt cgtgtagata actacgatac gggagggctt accatctggc
     4021 cccagtgctg caatgatacc gcgagaccca cgctcaccgg ctccagattt atcagcaata
     4081 aaccagccag ccggaagggc cgagcgcaga agtggtcctg caactttatc cgcctccatc
     4141 cagtctatta attgttgccg ggaagctaga gtaagtagtt cgccagttaa tagtttgcgc
     4201 aacgttgttg ccattgctac aggcatcgtg gtgtcacgct cgtcgtttgg tatggcttca
     4261 ttcagctccg gttcccaacg atcaaggcga gttacatgat cccccatgtt gtgcaaaaaa
     4321 gcggttagct ccttcggtcc tccgatcgtt gtcagaagta agttggccgc agtgttatca
     4381 ctcatggtta tggcagcact gcataattct cttactgtca tgccatccgt aagatgcttt
     4441 tctgtgactg gtgagtactc aaccaagtca ttctgagaat agtgtatgcg gcgaccgagt
     4501 tgctcttgcc cggcgtcaat acgggataat accgcgccac atagcagaac tttaaaagtg
     4561 ctcatcattg gaaaacgttc ttcggggcga aaactctcaa ggatcttacc gctgttgaga
     4621 tccagttcga tgtaacccac tcgtgcaccc aactgatctt cagcatcttt tactttcacc
     4681 agcgtttctg ggtgagcaaa aacaggaagg caaaatgccg caaaaaaggg aataagggcg
     4741 acacggaaat gttgaatact catactcttc ctttttcaat attattgaag catttatcag
     4801 ggttattgtc tcatgagcgg atacatattt gaatgtattt agaaaaataa acaaataggg
     4861 gttccgcgca catttccccg aaaagtgcca cctaaattgt aagcgttaat attttgttaa
     4921 aattcgcgtt aaatttttgt taaatcagct cattttttaa ccaataggcc gaaatcggca
     4981 aaatccctta taaatcaaaa gaatagaccg agatagggtt gagtgttgtt ccagtttgga
     5041 acaagagtcc actattaaag aacgtggact ccaacgtcaa agggcgaaaa accgtctatc
     5101 agggcgatgg cccactacgt gaaccatcac cctaatcaag ttttttgggg tcgaggtgcc
     5161 gtaaagcact aaatcggaac cctaaaggga gcccccgatt tagagcttga cggggaaagc
     5221 cggcgaacgt ggcgagaaag gaagggaaga aagcgaaagg agcgggcgct agggcgctgg
     5281 caagtgtagc ggtcacgctg cgcgtaacca ccacacccgc cgcgcttaat gcgccgctac
     5341 agggcgcgtc ccattcgcca ttcaggctgc gcaactgttg ggaagggcga tcggtgcggg
     5401 cctcttcgct attacgccag ctggcgaaag ggggatgtgc tgcaaggcga ttaagttggg
     5461 taacgccagg gttttcccag tcacgacgtt gtaaaacgac ggccagtgag cgcgcgtaat
     5521 acgactcact atagggcgaa ttggagctcc accgcggtgg cggccgctct agagagcttg
     5581 gcccattgca tacgttgtat ccatatcata atatgtacat ttatattggc tcatgtccaa
     5641 cattaccgcc atgttgacat tgattattga ctagttatta atagtaatca attacggggt
     5701 cattagttca tagcccatat atggagttcc gcgttacata acttacggta aatggcccgc
     5761 ctggctgacc gcccaacgac ccccgcccat tgacgtcaat aatgacgtat gttcccatag
     5821 taacgccaat agggactttc cattgacgtc aatgggtgga gtatttacgg taaactgccc
     5881 acttggcagt acatcaagtg tatcatatgc caagtacgcc ccctattgac gtcaatgacg
     5941 gtaaatggcc cgcctggcat tatgcccagt acatgacctt atgggacttt cctacttggc
     6001 agtacatcta cgtattagtc atcgctatta ccatggtgat gcggttttgg cagtacatca
     6061 atgggcgtgg atagcggttt gactcacggg gatttccaag tctccacccc attgacgtca
     6121 atgggagttt gttttggcac caaaatcaac gggactttcc aaaatgtcgt aacaactccg
     6181 ccccattgac gcaaatgggc ggtaggcgtg tacggtggga ggtctatata agcagagctc
     6241 gtttagtgaa ccgtcagatc gcctggagac gccatccacg ctgttttgac ctccatagaa
     6301 gacaccggga ccgatccagc ctcc
//
