LOCUS       sequence-457484-       11480 bp    DNA     circular SYN 14-APR-2025
DEFINITION  Mammalian expression of Csm complex from separate promoters; Puro 
            backbone (Used for RNA knockdown).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11480)
  AUTHORS   Colognori D, Trinidad M, Doudna JA
  TITLE     Precise transcript targeting by CRISPR-Csm complexes.
  JOURNAL   Nat Biotechnol. 2023 Jan 23. doi: 10.1038/s41587-022-01649-9.
  PUBMED    36690762
REFERENCE   2  (bases 1 to 11480)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 14, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Biotechnol. 2023 Jan 23. doi: 10.1038/s41587-022-01649-9."
COMMENT     Sequence Label: pDAC627
FEATURES             Location/Qualifiers
     source          1..11480
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     regulatory      92..101
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             101..124
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             131..151
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    539..586
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     promoter        617..820
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     regulatory      851..860
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             860..883
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             890..910
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    1580..1627
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     promoter        1658..1861
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     regulatory      1892..1901
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             1901..1924
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             1931..1951
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    2858..2905
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     promoter        2936..3139
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     regulatory      3170..3179
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             3179..3202
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             3209..3229
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    4310..4357
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     promoter        4388..4591
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     regulatory      4622..4631
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             4631..4654
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             4661..4681
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    5420..5467
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     promoter        5498..5701
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     CDS             5738..6337
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(5738..5757)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     6234..6254
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     polyA_signal    6338..6385
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     promoter        6416..6664
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for human U6 snRNA 
                     (Domitrovich & Kunkel, 2003)"
     primer_bind     6416..6436
                     /label=hU6-F
                     /note="Human U6 promoter, forward primer"
     primer_bind     6587..6606
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
     primer_bind     complement(6818..6836)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        6904..7008
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(7009..7077,7078..7869)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(7227..7246)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      8040..8628
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8529..8548
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     promoter        8659..8862
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     CDS             8902..8919
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             8926..8949
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             8956..8976
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    11260..11307
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     promoter        join(11338..11480,1..61)
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
ORIGIN
        1 cgccccattg acgcaaatgg gcggtaggcg tgtacggtgg gaggtctata taagcagagc
       61 tcgtttagtg aaccgtcaga tctctagagc cgccaccatg gattacaaag acgatgacga
      121 taagatggcg cctaagaaga aacgcaaagt gcggggcatg accatcctga ccgacgagaa
      181 ctacgtggac atcgccgaga aagccatcct gaagctggaa agaaacacca gaaatagaaa
      241 gaaccctgat gccttcttcc tgaccacatc taagctgcgg aacctgctga gcctgacaag
      301 caccctgttc gacgagagca aggtgaagga atacgacgcc ctgctggaca gaatcgctta
      361 tctgagagtg cagttcgtgt accaggccgg cagagagatc gccgtgaaag atctgatcga
      421 gaaggcccag atcctggaag ctctgaaaga gatcaaggac cgggaaaccc tgcagagatt
      481 ctgcagatac atggaagccc tggtggccta cttcaagttc tacggcggca aggactgacg
      541 gcaataaaaa gacagaataa aacgcacggt gttgggtcgt ttgttcagtt cttttgcctt
      601 actttcaatg catgcggtga tgcggttttg gcagtacatc aatgggcgtg gatagcggtt
      661 tgactcacgg ggatttccaa gtctccaccc cattgacgtc aatgggagtt tgttttggca
      721 ccaaaatcaa cgggactttc caaaatgtcg taacaactcc gccccattga cgcaaatggg
      781 cggtaggcgt gtacggtggg aggtctatat aagcagagct cgtttagtga accgtcagat
      841 ctctagagcc gccaccatgg attacaaaga cgatgacgat aagatggcgc ctaagaagaa
      901 acgcaaagtg cggggcatga ccttcgccaa gatcaaattc agcgcccaga tccggctgga
      961 aaccggcctg cacatcggag gatctgatgc ctttgccgct atcggcgcca tcgacagccc
     1021 tgtgatcaag gaccccatca ccaacctgcc tatcatcccc ggctctagcc tgaagggcaa
     1081 gatgagaaca ctgctggcca aggtgtacaa cgaaaaggtg gccgagaagc ctagcgacga
     1141 cagcgacatc ctgagcagac tgttcggaaa tagcaaggat aagcggttca agatgggcag
     1201 actgatcttc cgggacgcct tcctgagcaa cgccgacgag ctggattctc tgggcgtgcg
     1261 gagctacacc gaggtgaagt tcgagaacac catcgataga atcaccgccg aggccaatcc
     1321 tagacagatc gagagagcca ttcggaactc aacattcgac ttcgagctga tctacgagat
     1381 cactgatgag aatgagaacc aggtcgagga agatttcaag gtgatcagag acggcctgaa
     1441 gctgctggaa ctggactacc tgggcggaag cggctccaga ggctacggca aagtggcttt
     1501 tgagaacctg aaagccacca cagtgttcgg caactacgac gtgaaaaccc tgaacgagct
     1561 gctgaccgcc gaagtgtgac ggcaataaaa agacagaata aaacgcacgg tgttgggtcg
     1621 tttgttctgg attgcgagaa tggactagta gcaaactgtg atgcggtttt ggcagtacat
     1681 caatgggcgt ggatagcggt ttgactcacg gggatttcca agtctccacc ccattgacgt
     1741 caatgggagt ttgttttggc accaaaatca acgggacttt ccaaaatgtc gtaacaactc
     1801 cgccccattg acgcaaatgg gcggtaggcg tgtacggtgg gaggtctata taagcagagc
     1861 tcgtttagtg aaccgtcaga tctctagagc cgccaccatg gattacaaag acgatgacga
     1921 taagatggcg cctaagaaga aacgcaaagt gcggggcatg acttacaagc tctacattat
     1981 gacctttcaa aacgcccact tcggttccgg cactctggac tcatcgaagc tgaccttctc
     2041 cgcggataga atcttctcgg cactcgtgct cgaggctctg aagatgggaa agctcgacgc
     2101 cttcttggcc gaggccaacc aggataagtt cactctgacc gacgcgttcc cattccaatt
     2161 cggtcctttc ctgccgaaac cgattggtta ccccaagcac gaccagatcg accagtctgt
     2221 ggacgtgaag gaagtccgcc gccaagcgaa gctgtccaaa aagctccagt tcctggctct
     2281 ggaaaacgtc gacgactacc tgaacggaga gctgtttgag aatgaggaac acgccgtgat
     2341 cgacacagtg accaagaacc agccccataa agatgataat ctgtaccaag tggccaccac
     2401 tcggttctcg aacgacacct ccctttacgt gatcgccaac gaatccgatc tgctgaacga
     2461 actgatgagc agccttcagt actccgggct gggcggcaaa aggtcctcag gattcggcag
     2521 atttgagctg gacatccaga acattccctt ggaactgtcc gaccggctga cgaagaacca
     2581 cagcgacaag gtcatgtcac ttaccaccgc cctcccggtg gacgctgatc tcgaggaagc
     2641 gatggaagat ggccattacc tgttgaccaa gtcgtccgga ttcgcattct cccacgccac
     2701 caacgaaaac tatcggaagc aggacctgta caagttcgcc tccgggagca ccttcagcaa
     2761 gactttcgag ggacagatcg tggacgtgcg ccctctcgat ttccctcacg ccgtgctgaa
     2821 ctacgccaag ccgctgttct ttaagctcga agtctaacgg caataaaaag acagaataaa
     2881 acgcacggtg ttgggtcgtt tgttcgcaca ttcaaaaaca ggcaattgga caagcgtgat
     2941 gcggttttgg cagtacatca atgggcgtgg atagcggttt gactcacggg gatttccaag
     3001 tctccacccc attgacgtca atgggagttt gttttggcac caaaatcaac gggactttcc
     3061 aaaatgtcgt aacaactccg ccccattgac gcaaatgggc ggtaggcgtg tacggtggga
     3121 ggtctatata agcagagctc gtttagtgaa ccgtcagatc tctagagccg ccaccatgga
     3181 ttacaaagac gatgacgata agatggcgcc taagaagaaa cgcaaagtgc ggggcatgaa
     3241 aaatgactac cggaccttca agctgagcct gctgaccctg gctcctatcc acatcggcaa
     3301 cggcgagaag tacaccagca gagaattcat ctacgagaac aagaagttct acttccccga
     3361 catgggcaag ttctacaaca agatggtgga aaagagactg gccgagaagt tcgaggcctt
     3421 cctgatccag accagaccca acgccagaaa caaccggctg atttcttttc tgaacgacaa
     3481 cagaatcgcc gaaagatctt ttggcggcta cagcatcagt gaaaccggcc tggaatctga
     3541 taagaaccct aacagcgccg gagctatcaa cgaggtgaac aaattcatcc gggacgcctt
     3601 cggaaatcct tacatcccag gcagcagcct gaagggcgcc atccgcacca tcctgatgaa
     3661 caccacacct aagtggaaca acgagaacgc cgtgaacgac ttcggcagat tcccaaagga
     3721 aaacaagaac ctgatccctt ggggacctaa gaaaggcaag gaatacgacg acctgttcaa
     3781 cgccatcaga gtgtccgaca gcaagccctt cgacaacaaa agcctgatcc tcgtgcagaa
     3841 gtgggactac agcgccaaaa ccaacaaggc caagcctctg cctctgtaca gagagtctat
     3901 cagccctctg accaagatcg agttcgagat aacaacaacc actgatgagg ccggcagact
     3961 gatcgaggaa ctgggaaagc gggcccaggc cttttataag gactacaagg cctttttcct
     4021 gtctgaattc cctgatgata agatccaggc taatctgcaa taccccatct acctgggcgc
     4081 cggcagcggc gcttggacaa agaccctgtt taagcaggcc gacggcatcc tgcagcggag
     4141 atactccaga atgaaaacca agatggtcaa gaagggcgtg ctgaagctga caaaggcccc
     4201 tctgaaaaca gtgaagatcc ccagcggcaa ccacagcctg gtgaagaatc acgagagctt
     4261 ctacgagatg ggcaaagcca acttcatgat caaggaaatc gacaagtgac ggcaataaaa
     4321 agacagaata aaacgcacgg tgttgggtcg tttgttcatg tgtgccggcc ggagaataaa
     4381 agtctaagtg atgcggtttt ggcagtacat caatgggcgt ggatagcggt ttgactcacg
     4441 gggatttcca agtctccacc ccattgacgt caatgggagt ttgttttggc accaaaatca
     4501 acgggacttt ccaaaatgtc gtaacaactc cgccccattg acgcaaatgg gcggtaggcg
     4561 tgtacggtgg gaggtctata taagcagagc tcgtttagtg aaccgtcaga tctctagagc
     4621 cgccaccatg gattacaaag acgatgacga taagatggcg cctaagaaga aacgcaaagt
     4681 gcggggcatg aaaaagctcg tgttcacctt taagcggatc gaccaccctg ctcaggacct
     4741 ggccgtgaaa ttccacggct tcctgatgga acagctggat agcgactacg tggactacct
     4801 gcaccagcag cagaccaacc cctacgccac aaaggtgatc cagggcaaag agaacaccca
     4861 gtgggtcgtg catctgctga cagacgacat cgaggacaag gtgttcatga ccctgctgca
     4921 gatcaaggaa gtgtccctga acgacctgcc taagttgtct gtggaaaagg tggaaatcca
     4981 ggagctgggc gctgataagc tgctcgagat cttcaacagc gaggaaaacc agacctactt
     5041 cagcatcatc ttcgagacac ctacaggctt taaaagccag ggcagctacg tgatcttccc
     5101 cagcatgcgg ctgatctttc agagcctgat gcagaagtac ggcagactgg tggaaaacca
     5161 gcctgagatc gaggaagata ccctggacta cctgagcgag cacagcacca tcaccaatta
     5221 cagactggaa acaagctact tcagagtgca tagacagaga atccccgcct tccggggcaa
     5281 gctgaccttc aaggtgcagg gagcccagac actgaaggcc tacgtgaaga tgctgctgac
     5341 cttcggcgag tacagcggcc tgggcatgaa aaccagcctg ggaatgggcg gcatcaagct
     5401 ggaagaaaga aaggactgac ggcaataaaa agacagaata aaacgcacgg tgttgggtcg
     5461 tttgttcgtg cgatagaggg atcccgcatt gaattatgtg atgcggtttt ggcagtacat
     5521 caatgggcgt ggatagcggt ttgactcacg gggatttcca agtctccacc ccattgacgt
     5581 caatgggagt ttgttttggc accaaaatca acgggacttt ccaaaatgtc gtaacaactc
     5641 cgccccattg acgcaaatgg gcggtaggcg tgtacggtgg gaggtctata taagcagagc
     5701 tcgtttagtg aaccgtcaga tctctagagc cgccaccatg accgagtaca agcccacggt
     5761 gcgcctcgcc acccgcgacg acgtccccag ggccgtacgc accctcgccg ccgcgttcgc
     5821 cgactacccc gccacgcgcc acaccgtcga tccggaccgc cacatcgagc gggtcaccga
     5881 gctgcaagaa ctcttcctca cgcgcgtcgg gctcgacatc ggcaaggtgt gggtcgcgga
     5941 cgacggcgcc gcggtggcgg tctggaccac gccggagagc gtcgaagcgg gggcggtgtt
     6001 cgccgagatc ggcccgcgca tggccgagtt gagcggttcc cggctggccg cgcagcaaca
     6061 gatggaaggc ctcctggcgc cgcaccggcc caaggagccc gcgtggttcc tggccaccgt
     6121 cggagtctcg cccgaccacc agggcaaggg tctgggcagc gccgtcgtgc tccccggagt
     6181 ggaggcggcg gagcgcgccg gggtgcccgc cttcctggaa acctccgcgc cccgcaacct
     6241 ccccttctac gagcggctcg gcttcaccgt caccgccgac gtcgaggtgc ccgaaggacc
     6301 gcgcacctgg tgcatgaccc gcaagcccgg tgcctgacgg caataaaaag acagaataaa
     6361 acgcacggtg ttgggtcgtt tgttcgagca gattgtactg agagtgcacc ggttggaggg
     6421 cctatttccc atgattcctt catatttgca tatacgatac aaggctgtta gagagataat
     6481 tagaattaat ttgactgtaa acacaaagat attagtacaa aatacgtgac gtagaaagta
     6541 ataatttctt gggtagtttg cagttttaaa attatgtttt aaaatggact atcatatgct
     6601 taccgtaact tgaaagtatt tcgatttctt ggctttatat atcttgtgga aaggacgaaa
     6661 caccgatata aacctaatta cctcgagagg ggacggaaac ccgtcttcga tgaagcgatt
     6721 cagaagactt gatataaacc taattacctc gagaggggac ttttttacat gtgtcagagg
     6781 ttttcaccgt catcaccgaa acgcgcgaga cgaaagggcc tcgtgatacg cctattttta
     6841 taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat
     6901 gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg
     6961 agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa
     7021 catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac
     7081 ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac
     7141 atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt
     7201 ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tattgacgcc
     7261 gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca
     7321 ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc
     7381 ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag
     7441 gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa
     7501 ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgtagcaatg
     7561 gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa
     7621 ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg
     7681 gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt
     7741 gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt
     7801 caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag
     7861 cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat
     7921 ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct
     7981 taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct
     8041 tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca
     8101 gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc
     8161 agcagagcgc agataccaaa tactgttctt ctagtgtagc cgtagttagg ccaccacttc
     8221 aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct
     8281 gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag
     8341 gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc
     8401 tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg
     8461 agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag
     8521 cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt
     8581 gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac
     8641 gcggcctttt tcttaagcgt gatgcggttt tggcagtaca tcaatgggcg tggatagcgg
     8701 tttgactcac ggggatttcc aagtctccac cccattgacg tcaatgggag tttgttttgg
     8761 caccaaaatc aacgggactt tccaaaatgt cgtaacaact ccgccccatt gacgcaaatg
     8821 ggcggtaggc gtgtacggtg ggaggtctat ataagcagag ctcgtttagt gaaccgtcag
     8881 atctctagag ccgccaccat gcaccatcac catcaccatt ccggcgatta caaagacgat
     8941 gacgataaga tggcgcctaa gaagaaacgc aaagtgcggg gcatgaagaa agaaaagatt
     9001 gatctgtttt acggagccct gctgcacgac atcggaaagg tcatccagcg agcaaccgga
     9061 gagcggaaga aacacgcact tgtgggcgcc gactggttcg acgagatcgc cgacaaccaa
     9121 gtcatctcgg atcagatccg gtaccatatg gccaactacc agtctgataa gctcggcaac
     9181 gatcacctgg cttacatcac ctacattgcc gacaacatcg cctccggtgt cgaccgccgg
     9241 caatccaacg aagagtcaga cgaagatacc tccgcaaaga tctgggacac ctacacgaac
     9301 caggccgaca tctttaacgt gttcggagcg cagaccgata agcggtactt caagcctacc
     9361 gtgctgaatc tcaagtcgaa gcccaacttc gcgtccgcca cttacgaacc ctttagcaag
     9421 ggcgattacg ctgccatcgc cacccggatt aagaacgaac tggccgagtt cgagttcaac
     9481 caagtccaga ttgactccct gctcaacctt ttcgaggcta ctctctcctt cgtgccgtca
     9541 agcaccaaca ctaaggaaat cgccgacatc tccctggccg accattcccg cttgactgct
     9601 gccttcgctc tggcgatcta cgactacctg gaggacaagg gtcggcacaa ctacaaagag
     9661 gacctgttca ccaaagtgtc agcgttctat gaagaagaag ccttcctgct ggcctccttc
     9721 gacctgtcgg gaatccagga ctttatctac aacattaaca tcgcaactaa cggcgcggcg
     9781 aagcagctga aggcccggag cctctacctg gactttatgt ccgagtacat cgccgatagc
     9841 ctgctggaca agctgggact gaacagggct aacatgcttt acgtcggcgg cggacacgcc
     9901 tacttcgtcc tggccaacac cgaaaagact gtggaaaccc tggtgcagtt tgagaaggat
     9961 ttcaaccagt tcctgttggc aaacttccag acccgcctct atgtggcctt tggctggggt
    10021 tccttcgcgg ccaaggacat catgtccgag ctgaatagcc ccgagtccta ccgccaagtg
    10081 taccaaaagg cttcgcgcat gatctccaaa aagaaaatct ccagatacga ctaccagaca
    10141 ctgatgctcc tgaatcgcgg tggaaagtcc tcagagagag agtgcgagat ttgccactcc
    10201 gtggagaacc tggtgtccta ccacgaccag aaagtctgtg acatttgccg gggactgtac
    10261 cagttctcga aagaaattgc ccatgaccac ttcatcatta ccgaaaatga ggggctgccg
    10321 attggaccaa acgcgtgctt aaagggcgtg gcattcgaaa agctgtccca agaagcgttc
    10381 agccgggtct acgtgaagaa tgactataag gccggtaccg tgaaggctac gcatgtgttc
    10441 gtgggggatt accagtgcga cgagatctac aactacgccg ccctgagcaa gaacgagaac
    10501 ggcctaggca tcaagagact ggccgtggtc cggctcgacg tggatgactt gggcgccgcc
    10561 ttcatggccg gtttcagcca gcagggaaac ggacaatact ccactctgtc aagatcggcc
    10621 acattctccc ggagcatgtc gctgttcttc aaagtgtaca ttaaccagtt cgcctccgac
    10681 aagaagctga gcattatcta cgcgggcggc gatgacgtgt tcgccattgg atcgtggcag
    10741 gatatcatcg cgttcactgt ggaacttcgc gaaaacttca tcaagtggac caacgggaag
    10801 ctcaccctct ccgcggggat agggttgttc gccgacaaga ctcctattag cctgatggct
    10861 caccagaccg gggaactgga agaggccgcc aagggcaacg aaaaggactc catctcgctg
    10921 ttctcaagcg actacacttt caagtttgat aggttcatca ctaacgtgta cgacgacaaa
    10981 ctggaacaga ttagatactt cttcaaccat caagacgaga ggggaaagaa cttcatctat
    11041 aagcttattg agcttttgag gaaccacgac cgcatgaata tggcacgcct cgcctattac
    11101 ctcactcgcc tggaagaact gacccgggag actgacaggg acaagttcaa gaccttcaag
    11161 aacctgttct actcctggta caccaacaag aacgataagg accggaagga agccgagctc
    11221 gcgctcctgc tgtacatcta cgaaatcaga aaggattaac ggcaataaaa agacagaata
    11281 aaacgcacgg tgttgggtcg tttgttcgca cacattagct agccgtcagc acacattgtg
    11341 atgcggtttt ggcagtacat caatgggcgt ggatagcggt ttgactcacg gggatttcca
    11401 agtctccacc ccattgacgt caatgggagt ttgttttggc accaaaatca acgggacttt
    11461 ccaaaatgtc gtaacaactc
//
