LOCUS       sequence-457834-       10506 bp    DNA     circular SYN 14-APR-2025
DEFINITION  Lentiviral fluorescent ubiquitination-based cell cycle indicator 
            (FUCCI).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10506)
  AUTHORS   Liang WW, Muller S, Hart SK, Wessels HH, Mendez-Mancilla A, Sookdeo 
            A, Choi O, Caragine CM, Corman A, Lu L, Kolumba O, Williams B, 
            Sanjana NE
  TITLE     Transcriptome-scale RNA-targeting CRISPR screens reveal essential 
            lncRNAs in human cells.
  JOURNAL   Cell. 2024 Dec 26;187(26):7637-7654.e29. doi: 
            10.1016/j.cell.2024.10.021. Epub 2024 Nov 11.
  PUBMED    39532094
REFERENCE   2  (bases 1 to 10506)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 14, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2024.10.021"; journalName: "Cell"; date: 
            "2024-12-26- 26"; volume: "187"; issue: "26"; pages: "7637-7654.e29"
COMMENT     Sequence Label: pLentiFUCCI(CA)5
FEATURES             Location/Qualifiers
     source          1..10506
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     regulatory      92..101
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             1145..1201
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             1943..2272
                     /codon_start=1
                     /product="degron consisting of residues 1-110 of human 
                     Geminin (Zielke and Edgar, 2015)"
                     /label=Gem1 (1-110)
                     /translation="MNPSMKQKQEEIKENIKNSSVPRRTLKMIQPSASGSLVGRENELS
                     AGLSKRKHRNDHLTSTTSSPGVIVPESSENKNLGGVTQESFDLMIKENPSSQYWKEVAE
                     KRRKAL"
     primer_bind     complement(2489..2506)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     2716..2735
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     misc_feature    2799..3387
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(2852..2872)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(3270..3281)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             3459..3692
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     primer_bind     complement(3718..3735)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    3724..3948
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      3994..4422
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4081..4100)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     4291..4312
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(4431..4451)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        4436..4765
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      4616..4751
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     4678..4697
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        4813..4860
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             4879..5253
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    5383..5504
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5420..5439)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     5474..5493
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(5553..5569)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5553..5569)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(5566..5588)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    5577..5593
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(5601..5607,5608..5625,5626..5631))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    5646..5667
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(5784..5801)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(5955..6543)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6035..6054)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(6714..7505,7506..7574))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     7337..7356
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(7575..7679)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(7754..7773)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        7945..8324
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        8326..8524
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     8479..8499
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     LTR             8542..8722
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    8769..8894
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    9387..9620
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             9805..9849
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    10147..10264
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        join(10348..10506,1..53)
                     /label=EF-1-alpha core promoter
                     /note="core promoter for human elongation factor 
                     EF-1-alpha"
ORIGIN
        1 cagtagtcgc cgtgaacgtt ctttttcgca acgggtttgc cgccagaaca caggaccggc
       61 tggctaacta gaggagacgg ctagcggatc cgccaccatg gagaacgtca ggagaaagac
      121 aggcattcag acggaaatga aaacgaagct ccacatggat ggtatggtga atggacacag
      181 ctttgagatc aaaggggagg ggaagggaag cccgtacgag ggggtgcaga caatgaagct
      241 gaaggttact aagggcgccc cacttccctt ctctatagac attcttttgc ctcagtgcat
      301 gtatggcagc aaacctttca ttaaatatcc tgagaacatc cccgattatt tgaaactctc
      361 atttccagag ggtatcactt gggagaggac aatgacattc gaggacggcg cagtatgcga
      421 tgtatctaat gattccagac tggtcggcaa ttgctttatc tacactgtga agtttcaggg
      481 agtcaatttt ccacttgatg gccccgttat gcaaaagaag acccgaggct gggaaccctc
      541 cactgaagtg ctgtacgaat gtgatgggtg gatgcgcggg ttggtggaca tagctctgaa
      601 actggaaaac ggtggacatt atatgtgtaa ttttaagacc acctacaaat ccaagaaagg
      661 tcttgaggtg ccaccttacc atttcgtgga tcataaactc gacctgctca gtcataacac
      721 cgacggagcc acctttgagg agttcgaaca aggtgaaatc gctcacgcgc acttgagtaa
      781 actggccgga tatccatcac actggcggcc gctcgagatg gagcagcgcc gcgtcaccga
      841 cttcttcgcg cgccgccgcc ccgggccccc ccgcatcgcg ccgcccaagc tggcctgccg
      901 cacccccagc cccgccaggc ccgcactccg cgccccggcc tccgctacca gtggcagccg
      961 caagcgcgcc cgcccgcccg ccgcccccgg acgcgaccag gccaggccac cggcccgcgc
     1021 ggccgcgcgg ctgtcggtgg acgaggtttc cagccccagt acccccgagg ccccagacat
     1081 cccagcctgc ccttctccgg gccagaagat aaagaaaacc ggtcgcgcca agcgcggcag
     1141 cggcgccacc aacttcagcc tgctgaagca ggccggcgac gtggaggaga accccggccc
     1201 ccgcgaattc atgagtaaac tccagaaagg tgtggagaaa gagatgaaga ttaagctcac
     1261 tatgaactgc accgtgaacg aacggaactt tgtgatcaca gggcaggggg ctggggaacc
     1321 ttatgatgga actcagacac tttacctgac agtggagggc ggaaagacac tgaacttcag
     1381 cttcgatgta ctgaccccag cctttcagta cggcaatagg gcgtttacca agtaccccgg
     1441 taacatacca gacttcttca aacagacctt cagtggcggg ggatacacat ggaaaagaac
     1501 aatgacctat gaagacggcg gcgtctcaac cgttgagtcc gatatcagcg ttcaagggga
     1561 ctgcttccat tataaaatac aattcaacgg aaagttcccc ccccacggcc cagtcatgca
     1621 aaaggaaaca gtcaaatggg aaccttccac cgaggtgatg tacaaggacg ataaaaacga
     1681 tggagtgctg aagggcgacg tgaatatggc cctgctgttg aaggatggtg gtcacctcag
     1741 agtcgatttc aacacttctt atattcccaa aaataaggtg gagaagatgc cagattacca
     1801 cttcgtggac caccggatcg agatcctgga gaagcccgaa ggacgccctg tcaagctcta
     1861 cgcaggggct gtggccaggt acagcctgct gcctgaaaag aacctgaata agggatatcc
     1921 atcacactgg cggccgctcg agatgaatcc cagtatgaag cagaaacaag aagaaatcaa
     1981 agagaatata aagaatagtt ctgtcccaag aagaactctg aagatgattc agccttctgc
     2041 atctggatct cttgttggaa gagaaaatga gctgtccgca ggcttgtcca aaaggaaaca
     2101 tcggaatgac cacttaacat ctacaacttc cagccctggg gttattgtcc cagaatctag
     2161 tgaaaataaa aatcttggag gagtcaccca ggagtcattt gatcttatga ttaaagaaaa
     2221 tccatcctct cagtattgga aggaagtggc agaaaaacgg agaaaggcgc tgtaatctag
     2281 agttaactaa cttaagctag caacggtttc cctctagcgg gatcaattcc gccccccccc
     2341 cctaacgtta ctggccgaag ccgcttggaa taaggccggt gtgcgtttgt ctatatgtta
     2401 ttttccacca tattgccgtc ttttggcaat gtgagggccc ggaaacctgg ccctgtcttc
     2461 ttgacgagca ttcctagggg tctttcccct ctcgccaaag gaatgcaagg tctgttgaat
     2521 gtcgtgaagg aagcagttcc tctggaagct tcttgaagac aaacaacgtc tgtagcgacc
     2581 ctttgcaggc agcggaaccc cccacctggc gacaggtgcc tctgcggcca aaagccacgt
     2641 gtataagata cacctgcaaa ggcggcacaa ccccagtgcc acgttgtgag ttggatagtt
     2701 gtggaaagag tcaaatggct ctcctcaagc gtattcaaca aggggctgaa ggatgcccag
     2761 aaggtacccg tctctaagga ctctcgcgtt aagtcgacaa tcaacctctg gattacaaaa
     2821 tttgtgaaag attgactggt attcttaact atgttgctcc ttttacgcta tgtggatacg
     2881 ctgctttaat gcctttgtat catgctattg cttcccgtat ggctttcatt ttctcctcct
     2941 tgtataaatc ctggttgctg tctctttatg aggagttgtg gcccgttgtc aggcaacgtg
     3001 gcgtggtgtg cactgtgttt gctgacgcaa cccccactgg ttggggcatt gccaccacct
     3061 gtcagctcct ttccgggact ttcgctttcc ccctccctat tgccacggcg gaactcatcg
     3121 ccgcctgcct tgcccgctgc tggacagggg ctcggctgtt gggcactgac aattccgtgg
     3181 tgttgtcggg gaaatcatcg tcctttcctt ggctgctcgc ctgtgttgcc acctggattc
     3241 tgcgcgggac gtccttctgc tacgtccctt cggccctcaa tccagcggac cttccttccc
     3301 gcggcctgct gccggctctg cggcctcttc cgcgtcttcg ccttcgccct cagacgagtc
     3361 ggatctccct ttgggccgcc tccccgcgtc gactttaaga ccaatgactt acaaggcagc
     3421 tgtagatctt agccactttt taaaagaaaa ggggggactg gaagggctaa ttcactccca
     3481 acgaagacaa gatctgcttt ttgcttgtac tgggtctctc tggttagacc agatctgagc
     3541 ctgggagctc tctggctaac tagggaaccc actgcttaag cctcaataaa gcttgccttg
     3601 agtgcttcaa gtagtgtgtg cccgtctgtt gtgtgactct ggtaactaga gatccctcag
     3661 acccttttag tcagtgtgga aaatctctag cagggcccgt ttaaacccgc tgatcagcct
     3721 cgactgtgcc ttctagttgc cagccatctg ttgtttgccc ctcccccgtg ccttccttga
     3781 ccctggaagg tgccactccc actgtccttt cctaataaaa tgaggaaatt gcatcgcatt
     3841 gtctgagtag gtgtcattct attctggggg gtggggtggg gcaggacagc aagggggagg
     3901 attgggaaga caatagcagg catgctgggg atgcggtggg ctctatggct tctgaggcgg
     3961 aaagaaccag ctggggctct agggggtatc cccacgcgcc ctgtagcggc gcattaagcg
     4021 cggcgggtgt ggtggttacg cgcagcgtga ccgctacact tgccagcgcc ctagcgcccg
     4081 ctcctttcgc tttcttccct tcctttctcg ccacgttcgc cggctttccc cgtcaagctc
     4141 taaatcgggg gctcccttta gggttccgat ttagtgcttt acggcacctc gaccccaaaa
     4201 aacttgatta gggtgatggt tcacgtagtg ggccatcgcc ctgatagacg gtttttcgcc
     4261 ctttgacgtt ggagtccacg ttctttaata gtggactctt gttccaaact ggaacaacac
     4321 tcaaccctat ctcggtctat tcttttgatt tataagggat tttgccgatt tcggcctatt
     4381 ggttaaaaaa tgagctgatt taacaaaaat ttaacgcgaa ttaattctgt ggaatgtgtg
     4441 tcagttaggg tgtggaaagt ccccaggctc cccagcaggc agaagtatgc aaagcatgca
     4501 tctcaattag tcagcaacca ggtgtggaaa gtccccaggc tccccagcag gcagaagtat
     4561 gcaaagcatg catctcaatt agtcagcaac catagtcccg cccctaactc cgcccatccc
     4621 gcccctaact ccgcccagtt ccgcccattc tccgccccat ggctgactaa ttttttttat
     4681 ttatgcagag gccgaggccg cctctgcctc tgagctattc cagaagtagt gaggaggctt
     4741 ttttggaggc ctaggctttt gcaaaaagct cccgggagct tgtatatcca ttttcggatc
     4801 tgatcagcac gtgttgacaa ttaatcatcg gcatagtata tcggcatagt ataatacgac
     4861 aaggtgagga actaaaccat ggccaagttg accagtgccg ttccggtgct caccgcgcgc
     4921 gacgtcgccg gagcggtcga gttctggacc gaccggctcg ggttctcccg ggacttcgtg
     4981 gaggacgact tcgccggtgt ggtccgggac gacgtgaccc tgttcatcag cgcggtccag
     5041 gaccaggtgg tgccggacaa caccctggcc tgggtgtggg tgcgcggcct ggacgagctg
     5101 tacgccgagt ggtcggaggt cgtgtccacg aacttccggg acgcctccgg gccggccatg
     5161 accgagatcg gcgagcagcc gtgggggcgg gagttcgccc tgcgcgaccc ggccggcaac
     5221 tgcgtgcact tcgtggccga ggagcaggac tgacacgtgc tacgagattt cgattccacc
     5281 gccgccttct atgaaaggtt gggcttcgga atcgttttcc gggacgccgg ctggatgatc
     5341 ctccagcgcg gggatctcat gctggagttc ttcgcccacc ccaacttgtt tattgcagct
     5401 tataatggtt acaaataaag caatagcatc acaaatttca caaataaagc atttttttca
     5461 ctgcattcta gttgtggttt gtccaaactc atcaatgtat cttatcatgt ctgtataccg
     5521 tcgacctcta gctagagctt ggcgtaatca tggtcatagc tgtttcctgt gtgaaattgt
     5581 tatccgctca caattccaca caacatacga gccggaagca taaagtgtaa agcctggggt
     5641 gcctaatgag tgagctaact cacattaatt gcgttgcgct cactgcccgc tttccagtcg
     5701 ggaaacctgt cgtgccagct gcattaatga atcggccaac gcgcggggag aggcggtttg
     5761 cgtattgggc gctcatccgc ttcctcgctc actgactcgc tgcgctcggt cgttcggctg
     5821 cggcgagcgg tatcagctca ctcaaaggcg gtaatacggt tatccacaga atcaggggat
     5881 aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc
     5941 gcgttgctgg cgtttttcca taggctccgc ccccctgacg agcatcacaa aaatcgacgc
     6001 tcaagtcaga ggtggcgaaa cccgacagga ctataaagat accaggcgtt tccccctgga
     6061 agctccctcg tgcgctctcc tgttccgacc ctgccgctta ccggatacct gtccgccttt
     6121 ctcccttcgg gaagcgtggc gctttctcat agctcacgct gtaggtatct cagttcggtg
     6181 taggtcgttc gctccaagct gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc
     6241 gccttatccg gtaactatcg tcttgagtcc aacccggtaa gacacgactt atcgccactg
     6301 gcagcagcca ctggtaacag gattagcaga gcgaggtatg taggcggtgc tacagagttc
     6361 ttgaagtggt ggcctaacta cggctacact agaagaacag tatttggtat ctgcgctctg
     6421 ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa acaaaccacc
     6481 gctggtagcg gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct
     6541 caagaagatc ctttgatctt ttctacgggg tctgacgctc agtggaacga aaactcacgt
     6601 taagggattt tggtcatgag attatcaaaa aggatcttca cctagatcct tttaaattaa
     6661 aaatgaagtt ttaaatcaat ctaaagtata tatgagtaaa cttggtctga cagttaccaa
     6721 tgcttaatca gtgaggcacc tatctcagcg atctgtctat ttcgttcatc catagttgcc
     6781 tgactccccg tcgtgtagat aactacgata cgggagggct taccatctgg ccccagtgct
     6841 gcaatgatac cgcgagaccc acgctcaccg gctccagatt tatcagcaat aaaccagcca
     6901 gccggaaggg ccgagcgcag aagtggtcct gcaactttat ccgcctccat ccagtctatt
     6961 aattgttgcc gggaagctag agtaagtagt tcgccagtta atagtttgcg caacgttgtt
     7021 gccattgcta caggcatcgt ggtgtcacgc tcgtcgtttg gtatggcttc attcagctcc
     7081 ggttcccaac gatcaaggcg agttacatga tcccccatgt tgtgcaaaaa agcggttagc
     7141 tccttcggtc ctccgatcgt tgtcagaagt aagttggccg cagtgttatc actcatggtt
     7201 atggcagcac tgcataattc tcttactgtc atgccatccg taagatgctt ttctgtgact
     7261 ggtgagtact caaccaagtc attctgagaa tagtgtatgc ggcgaccgag ttgctcttgc
     7321 ccggcgtcaa tacgggataa taccgcgcca catagcagaa ctttaaaagt gctcatcatt
     7381 ggaaaacgtt cttcggggcg aaaactctca aggatcttac cgctgttgag atccagttcg
     7441 atgtaaccca ctcgtgcacc caactgatct tcagcatctt ttactttcac cagcgtttct
     7501 gggtgagcaa aaacaggaag gcaaaatgcc gcaaaaaagg gaataagggc gacacggaaa
     7561 tgttgaatac tcatactctt cctttttcaa tattattgaa gcatttatca gggttattgt
     7621 ctcatgagcg gatacatatt tgaatgtatt tagaaaaata aacaaatagg ggttccgcgc
     7681 acatttcccc gaaaagtgcc acctgacgtc gacggatcgg gagatctccc gatcccctat
     7741 ggtgcactct cagtacaatc tgctctgatg ccgcatagtt aagccagtat ctgctccctg
     7801 cttgtgtgtt ggaggtcgct gagtagtgcg cgagcaaaat ttaagctaca acaaggcaag
     7861 gcttgaccga caattgcatg aagaatctgc ttagggttag gcgttttgcg ctgcttcgcg
     7921 atgtacgggc cagatatacg cgttgacatt gattattgac tagttattaa tagtaatcaa
     7981 ttacggggtc attagttcat agcccatata tggagttccg cgttacataa cttacggtaa
     8041 atggcccgcc tggctgaccg cccaacgacc cccgcccatt gacgtcaata atgacgtatg
     8101 ttcccatagt aacgccaata gggactttcc attgacgtca atgggtggag tatttacggt
     8161 aaactgccca cttggcagta catcaagtgt atcatatgcc aagtacgccc cctattgacg
     8221 tcaatgacgg taaatggccc gcctggcatt atgcccagta catgacctta tgggactttc
     8281 ctacttggca gtacatctac gtattagtca tcgctattac catggtgatg cggttttggc
     8341 agtacatcaa tgggcgtgga tagcggtttg actcacgggg atttccaagt ctccacccca
     8401 ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg ggactttcca aaatgtcgta
     8461 acaactccgc cccattgacg caaatgggcg gtaggcgtgt acggtgggag gtctatataa
     8521 gcagcgcgtt ttgcctgtac tgggtctctc tggttagacc agatctgagc ctgggagctc
     8581 tctggctaac tagggaaccc actgcttaag cctcaataaa gcttgccttg agtgcttcaa
     8641 gtagtgtgtg cccgtctgtt gtgtgactct ggtaactaga gatccctcag acccttttag
     8701 tcagtgtgga aaatctctag cagtggcgcc cgaacaggga cttgaaagcg aaagggaaac
     8761 cagaggagct ctctcgacgc aggactcggc ttgctgaagc gcgcacggca agaggcgagg
     8821 ggcggcgact ggtgagtacg ccaaaaattt tgactagcgg aggctagaag gagagagatg
     8881 ggtgcgagag cgtcagtatt aagcggggga gaattagatc gcgatgggaa aaaattcggt
     8941 taaggccagg gggaaagaaa aaatataaat taaaacatat agtatgggca agcagggagc
     9001 tagaacgatt cgcagttaat cctggcctgt tagaaacatc agaaggctgt agacaaatac
     9061 tgggacagct acaaccatcc cttcagacag gatcagaaga acttagatca ttatataata
     9121 cagtagcaac cctctattgt gtgcatcaaa ggatagagat aaaagacacc aaggaagctt
     9181 tagacaagat agaggtagag caaaacaaaa gtaagaccac cgcacagcaa gcggccgctg
     9241 atcttcagac ctggaggagg agatatgagg gacaattgga gaagtgaatt atataaatat
     9301 aaagtagtaa aaattgaacc attaggagta gcacccacca aggcaaagag aagagtggtg
     9361 cagagagaaa aaagagcagt gggaatagga gctttgttcc ttgggttctt gggagcagca
     9421 ggaagcacta tgggcgcagc gtcaatgacg ctgacggtac aggccagaca attattgtct
     9481 ggtatagtgc agcagcagaa caatttgctg agggctattg aggcgcaaca gcatctgttg
     9541 caactcacag tctggggcat caagcagctc caggcaagaa tcctggctgt ggaaagatac
     9601 ctaaaggatc aacagctcct ggggatttgg ggttgctctg gaaaactcat ttgcaccact
     9661 gctgtgcctt ggaatgctag ttggagtaat aaatctctgg aacagatttg gaatcacacg
     9721 acctggatgg agtgggacag agaaattaac aattacacaa gcttaataca ctccttaatt
     9781 gaagaatcgc aaaaccagca agaaaagaat gaacaagaat tattggaatt agataaatgg
     9841 gcaagtttgt ggaattggtt taacataaca aattggctgt ggtatataaa attattcata
     9901 atgatagtag gaggcttggt aggtttaaga atagtttttg ctgtactttc tatagtgaat
     9961 agagttaggc agggatattc accattatcg tttcagaccc acctcccaac cccgagggga
    10021 cccgacaggc ccgaaggaat agaagaagaa ggtggagaga gagacagaga cagatccatt
    10081 cgattagtga acggatcggc actgcgtgcg ccaattctgc agacaaatgg cagtattcat
    10141 ccacaatttt aaaagaaaag gggggattgg ggggtacagt gcaggggaaa gaatagtaga
    10201 cataatagca acagacatac aaactaaaga attacaaaaa caaattacaa aaattcaaaa
    10261 ttttcgggtt tattacaggg acagcagaga tccagtttgg ttaattaatc ttgaaaggag
    10321 tgggaattga ctccggtgcc cgtcagtggg cagagcgcac atcgcccaca gtccccgaga
    10381 agttgggggg aggggtcggc aattgatccg gtgcctagag aaggtggcgc ggggtaaact
    10441 gggaaagtga tgtcgtgtac tggctccgcc tttttcccga gggtggggga gaaccgtata
    10501 taagtg
//
