LOCUS       sequence-457850-        1098 bp    DNA     linear   UNA 15-APR-2025
DEFINITION  Mammalian expression of GNL2 (593-660aa).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1098)
  TITLE     Iuchi Lab GNL2 Plasmids 2025
REFERENCE   2  (bases 1 to 1098)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pGNL2-#26
FEATURES             Location/Qualifiers
     source          1..1098
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     polyA_signal    343..464
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(380..399)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     434..453
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      complement(471..926)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(608..629)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     820..839
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
ORIGIN
        1 gnccgnattc nngctcagct tcgattcacc aaagccgtta ttaaagcact ggatgagaag
       61 attgccaaat atcagaagtt tctagacaaa gccaaagcca aaaagttttc agcagtcaga
      121 atatccaagg gactgagtga aaagatattt gcaaaacctg aagaacaaag aaaaacactg
      181 gaagaagatg tagatgacag agcaccttcc aaaaagggaa agaagcggaa gtaactgatc
      241 ataatcagcc ataccacatt tgtagaggtt ttacttgctt taaaaaacct cccacacctc
      301 cccctgaacc tgaaacataa aatgaatgca attgttgttg ttaacttgtt tattgcagct
      361 tataatggtt acaaataaag caatagcatc acaaatttca caaataaagc atttttttca
      421 ctgcattcta gttgtggttt gtccaaactc atcaatgtat cttaacgcgt aaattgtaag
      481 cgttaatatt ttgttaaaat tcgcgttaaa tttttgttaa atcagctcat tttttaacca
      541 ataggccgaa atcggcaaaa tcccttataa atcaaaagaa tagaccgaga tagggttgag
      601 tgttgttcca gtttggaaca agagtccact attaaagaac gtggactcca acgtcaaagg
      661 gcgaaaaacc gtctatcagg gcgatggccc actacgtgaa ccatcaccct aatcaagttt
      721 tttggggtcg aggtgccgta aagcactaaa tcggaaccct aaagggagcc cccgatttag
      781 agcttgacgg ggaaagccgg cgaacgtggc gagaaaggaa gggaagaaag cgaaaggagc
      841 gggcgctagg gcgctggcaa gtgtagcggt cacgctgcgc gtaaccacca cacccgccgc
      901 gcttaatgcg ccgctacagg gcgcgtcann ggcacttttc ggggnntgtg cgcggaaccc
      961 tattgtttat ttttctaana cattcanatg tatccgctca tgagacaata cctgataatt
     1021 gcttcanana ttgaaannnn nagtctgagg cggaannann ncctgtgnnn tgtgtgtcag
     1081 taggtgtgnn nnnnccca
//
