LOCUS       sequence-457856-        1092 bp    DNA     linear   UNA 15-APR-2025
DEFINITION  Bacterial expression of His-tagged GNL2 (643-731aa). To be used as a
            probe..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1092)
  TITLE     Iuchi Lab GNL2 Plasmids 2025
REFERENCE   2  (bases 1 to 1092)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pGNL2-#20
FEATURES             Location/Qualifiers
     source          1..1092
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     670..692
                     /label=pGEX 5'
                     /note="pGEX vectors, Glutathione-S-transferase, forward 
                     primer"
     CDS             722..733
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     CDS             1016..1033
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
ORIGIN
        1 tcggntnann tgtggnnttg tgancgataa caatttcaca cannacagta ttcnnncnta
       61 tactaaggtt ntggaanntt anggncnngt gcaaacccac ctcgaantct tttggaataa
      121 tcttgaanaa aaaaatatga agagcatttg tatgagcgcg atgaaaggtg ataaatggcg
      181 aaacaaaaag tttgaatttg gnttgggagt ttcccaatct tcccttatta tattgatggt
      241 gatgttaaat taacacagtc tatgggccat catacgttat atagctgaca agcacaacat
      301 gttgggtggt tgtccaaaag agcgtgcaga gatttcaatg cttgaaggag cggttttgga
      361 tattagatac ggtgtttcga gaattgcata tagtaaagac tttgaaactc tcaaagttga
      421 ttttcttagc aagctacctg aaatgctgaa aatgttcgaa gatcgtttat gtcataaaac
      481 atatttaaat ggtgatcatg taacccatcc tgacttcatg ttgtatgacg ctcttgatgt
      541 tgttttatac atggacccaa tgtgcctgga tgcgttccca aaattagttt gttttaaaaa
      601 acgtattgaa gctatcccac aaattgataa gtacttgaaa tccagcaagt atatagcatg
      661 gcctttgcag ggctggcaag ccacgtttgg tggtggcgac catcctccaa aatcggatct
      721 gatcgaaggt cgtgggatcc ccgaattcct ggaagaagat gtagatgaca gagcaccttc
      781 caaaaaggga aagaagcgga aggcacaaag ggaagaggaa caggaacatt caaataaagc
      841 tcccagggcg cttacatcaa aagaacggag gcgagcagta cgacagcaac ggccgaaaaa
      901 agttggtgtg cgctactatg aaacacacaa cgtgaaaaat aggaacagga acaaaaagaa
      961 gaccaatgac tcagagggac agaaacacaa acgcaaaaaa ttcagacaaa agcagcacca
     1021 ccaccaccac cactgaccgc gactcgagcg gccgcatcgt gactgactga cgatcctgcc
     1081 tncncggngt ga
//
