LOCUS       sequence-457857-        1094 bp    DNA     linear   UNA 15-APR-2025
DEFINITION  Bacterial expression of 6xHis tag. To be used as a probe..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1094)
  TITLE     Iuchi Lab GNL2 Plasmids 2025
REFERENCE   2  (bases 1 to 1094)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pGNL2-#22
FEATURES             Location/Qualifiers
     source          1..1094
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        join(275..280,281..296,297..303)
                     /label=tac promoter
                     /note="strong E. coli promoter; hybrid between the trp and 
                     lac UV5 promoters"
     protein_bind    311..327
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     316..338
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     CDS             350..1003
                     /codon_start=1
                     /product="glutathione S-transferase from Schistosoma 
                     japonicum"
                     /label=GST
                     /translation="MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKK
                     FELGLEFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVLDIRY
                     GVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTHPDFMLYDALDVVL
                     YMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIAWPLQGWQATFGGGDHPPK"
     primer_bind     961..983
                     /label=pGEX 5'
                     /note="pGEX vectors, Glutathione-S-transferase, forward 
                     primer"
     CDS             1013..1024
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     CDS             1034..1051
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
ORIGIN
        1 attccgncng nntgntnntc gntcacnttn ntntgatgan agctggctac ngaaggccag
       61 acgcgnntat tttgatgcnn naanttaggc tattcgactg cacgatgcac caatgcttct
      121 gacgttcagg canccatcgg aaagctgtgg tatgagctgt gcaggtcgta atcactgcat
      181 aatttcgtgt cgctcaaggc gcacttcccg ttctggataa tgttttttgc gccgacatca
      241 taacgggttc tggcaaatat tctgaaatga gctgttgaca attaatcatc ggctcgtata
      301 atgtgtggaa ttgtgagcgg ataacaattt cacacaggaa acagtattca tgtcccctat
      361 actaggttat tggaaaatta agggccttgt gcaacccact cgacttcttt tggaatatct
      421 tgaagaaaaa tatgaagagc atttgtatga gcgcgatgaa ggtgataaat ggcgaaacaa
      481 aaagtttgaa ttgggtttgg agtttcccaa tcttccttat tatattgatg gtgatgttaa
      541 attaacacag tctatggcca tcatacgtta tatagctgac aagcacaaca tgttgggtgg
      601 ttgtccaaaa gagcgtgcag agatttcaat gcttgaagga gcggttttgg atattagata
      661 cggtgtttcg agaattgcat atagtaaaga ctttgaaact ctcaaagttg attttcttag
      721 caagctacct gaaatgctga aaatgttcga agatcgttta tgtcataaaa catatttaaa
      781 tggtgatcat gtaacccatc ctgacttcat gttgtatgac gctcttgatg ttgttttata
      841 catggaccca atgtgcctgg atgcgttccc aaaattagtt tgttttaaaa aacgtattga
      901 agctatccca caaattgata agtacttgaa atccagcaag tatatagcat ggcctttgca
      961 gggctggcaa gccacgtttg gtggtggcga ccatcctcca aaatcggatc tgatcgaagg
     1021 tcgtgggatc ccccaccacc accaccacca ctgagccgca tcgtgactga ctgacgatct
     1081 gccctcgcnn ncgt
//
