LOCUS       sequence-457913-        2848 bp    DNA     linear   UNA 15-APR-2025
DEFINITION  AAV transfer plasmid for CAG-mediated Cre-dependent expression of 
            soma-targeted FRCaMPi.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2848)
  TITLE     A Sensitive Soma-localized Red Fluorescent Calcium Indicator for 
            Multi-Modality Imaging of Neuronal Populations In Vivo
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2848)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pAAV-CAG-DIO-NES-SomaFRCaMPi
FEATURES             Location/Qualifiers
     source          1..2848
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    68..101
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     protein_bind    172..205
                     /label=lox2272
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (AAGTATCC) (Shaw et al., 2021). lox2272 sites are 
                     compatible with each other, but incompatible with loxP or 
                     loxN sites (Lee and Saito, 1988)."
     CDS             240..266
                     /codon_start=1
                     /product="nuclear export signal from the HIV Rev protein 
                     (Fischer et al., 1995)"
                     /label=NES
                     /translation="LPPLERLTL"
     primer_bind     689..708
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     protein_bind    complement(2340..2373)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     protein_bind    complement(2436..2469)
                     /label=lox2272
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (AAGTATCC) (Shaw et al., 2021). lox2272 sites are 
                     compatible with each other, but incompatible with loxP or 
                     loxN sites (Lee and Saito, 1988)."
     primer_bind     complement(2504..2523)
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     primer_bind     complement(2585..2607)
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
ORIGIN
        1 caaattttgt aatccagagg ttgattatcg ataagcttga tatcgaattc gaacctcttc
       61 gagggacata acttcgtata atgtatgcta tacgaagtta tgggtcgatg gtgagatctg
      121 gactagaggg tcgatggtga tgcttggatc aaaataggaa gaatcccaat gataacttcg
      181 tataaagtat cctatacgaa gttatactag aattcggtac ctaagccacc atgcttcaac
      241 ttcctcctct tgaacgtctt actctttcga gatctatggt gagcaagggc gaggaggata
      301 acatggccat catcaaggag ttcatgcgct tcaaggtgca catggagggc tccgtgaacg
      361 gccacgagtt cgagatcgag ggcgagggcg agggccgccc ctacgaggcc ttccagaccg
      421 ctaggctgaa ggtgaccaag ggtggccccc tgcccttcgc ctgggacatc ctgtcccctc
      481 agttcatgta cggctccaag gcctacatta agcacccagc cgacatcccc gactacttca
      541 agctgtcctt ccccgagggc ttcaggtggg agcgcgtgat gagcttcgag gacggcggca
      601 ttattcacgt taatcaggac tcctccctgc aggacggcgt attcatctac aaggtgaagc
      661 tgctcggcac caacttcccc cccgacggcc ccgtaatgca gaagaagacc atgggctggg
      721 aggcttccta cgagatgact acccgtaacc ttacagatga gcagattgcg gagttccgtg
      781 aggccttttc gctgcttgat cgtgatcagg atggaaatat cacgtccaat gaattgggtg
      841 tggttatgag gtcgttaggt ctatcgccta ctgccgccga attacaagat atgattaatg
      901 aggtcgatgc cgatggtaat ggcacaattg attttaccga atttttgaca atgatggccc
      961 ggaaaatgaa ggataccgac gacgaagagg aagttcgcga agcctttaaa gtcttcgata
     1021 aagatggaag tggatacatt acagtcgagg agctgactca tgttcttaca agtctcggtg
     1081 aacgtttgtc tcgagaagaa gtagccgatg tgatacgtga agccgactcc gatggcgatg
     1141 gtgtaatcaa ctacgaagaa ttttctcgtg tcatttcttc caagggcggt tcaggaggca
     1201 gcgggtcagg ttcaatgagg aaaccgttcc gtggcgcggg caacgctgtg cgtgctttca
     1261 gcacttggaa aaagctagtg ccgcgggttt ccgagtggat gtaccccgag gacggcgccc
     1321 tgaagagcga gatcaggaag gggctgaggc tgaaggacgg cggccactac gccgccgagg
     1381 tcaagaccac ctacaaggcc aagaagcccg tgcagctgcc cggcgcctac atcgtcgaca
     1441 tcgagttgga catcttgtcc cacaacgagg actacaccat cgtggaacag tgcgaacgcg
     1501 ccgtgggccg ccaccccacc ggtggcacgg acgcgctgta caagggcgga tctggcggta
     1561 gcggagggac aggtggctct ggagggagcg gaggcactgg cggatctggt gggagcggag
     1621 gtacctcagg ccggactcag atctcaagct caagcttcga gttcagcagc aaagtctcac
     1681 gcgacaccct gtacgaggcg gtgcgggaag tcctgcacgg gaaccagcgc aagcgccgca
     1741 agtttctgga gacggtggag ctgcagatca gcctgaagaa ctacgaccct cagaaggaca
     1801 aacgtttctc gggcaccgtc aggctcaagt ccaccccacg ccccaagttc tcggtgtgcg
     1861 ttctggggga ccagcagcac tgtgatgaag ccaaggccgt ggatatcccc cacatggaca
     1921 tcgaggcgct caagaaactt aacaaaaaca agaagttggt caagaagctg gctaagaagt
     1981 acgatgcctt tttggcctct gagtctctga ttaagcagat cccacgtatc ctgggcccag
     2041 gcctaaacaa ggctggcaag ttcccctccc tgctgacaca caatgaaaac atggtggcca
     2101 aagtggatga ggtgaaatcg acaatcaagt tccagatgaa gaaggtgctg tgtttggccg
     2161 tcgctgttgg ccacgtgaag atgaccgatg atgagctagt ctacaacatt catctggctg
     2221 tcaatttctt ggtgtccttg cttaagaaaa actggcaaaa cgtgcgggct ctgtacatca
     2281 agagcaccat gggcaagccc cagcgtctgt attagtgtac aagtaagggg atccctagta
     2341 taacttcgta tagcatacat tatacgaagt tatgggtcga tggtgatgct tggcaattcg
     2401 ggtcgatggt gaagcattgg tcttcctatt ttgatataac ttcgtatagg atactttata
     2461 cgaagttatg gggatccaat tctttgccaa aatgatgaga cagcacaata accagcacgt
     2521 tgcccaggag ctgtaggaaa aagaagaagg catgaacatg gttagcagag gctctagagc
     2581 cgccggtcac acgccagaag ccgaaccccg ccctgccccg tcccccccga aggcagccgt
     2641 ccccccgcgg acagccccga gccgctcccc ccgcacaaag ggccctcccg gagcccctca
     2701 aggctttcac gcagccacag aaaagaaaca agccgtcatt aaaccaagcg ctaattacag
     2761 cccggaggag aagggccgtc ccgcccgctc acctgtggga gtaacgcggt cagtcagagc
     2821 cggggcggcg gggccccaaa ggcggcgc
//
