LOCUS       sequence-458103-       16392 bp    DNA     circular SYN 15-APR-2025
DEFINITION  PiggyBac transposon vector containing a dox inducible mutant p300 
            core-dCas9 fusion protein (p300 core mutation: D1399Y) and EGFP 
            reporter. Hygromycin selection marker..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 16392)
  TITLE     PiggyBac CRISPR-dCas9 fusion protein and gRNA donor plasmids
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 16392)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: PB_Mut(D1399Y)_p300_core-dCas9-EGFP_hygro
FEATURES             Location/Qualifiers
     source          1..16392
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(join(171..962,963..1031))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     794..813
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(1032..1136)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(1162..1617)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(1299..1320)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     1511..1530
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     1744..1766
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     1758..1775
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     1759..1775
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     repeat_region   1905..1967
                     /label=piggyBac right (3') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
     protein_bind    2405..2423
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2440..2458
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2476..2494
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2512..2530
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2547..2565
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2583..2601
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     promoter        2613..2651
                     /label=minimal CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     2648..2672
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     CDS             2710..4560
                     /codon_start=1
                     /gene="EP300"
                     /product="catalytic histone acetyltransferase core of the 
                     human E1A-associated protein p300, inactivated by the 
                     D1399Y point mutation (Hilton et al., 2015)"
                     /label=p300 core (D1399Y)
                     /translation="IFKPEELRQALMPTLEALYRQDPESLPFRQPVDPQLLGIPDYFDI
                     VKSPMDLSTIKRKLDTGQYQEPWQYVDDIWLMFNNAWLYNRKTSRVYKYCSKLSEVFEQ
                     EIDPVMQSLGYCCGRKLEFSPQTLCCYGKQLCTIPRDATYYSYQNRYHFCEKCFNEIQG
                     ESVSLGDDPSQPQTTINKEQFSKRKNDTLDPELFVECTECGRKMHQICVLHHEIIWPAG
                     FVCDGCLKKSARTRKENKFSAKRLPSTRLGTFLENRVNDFLRRQNHPESGEVTVRVVHA
                     SDKTVEVKPGMKARFVDSGEMAESFPYRTKALFAFEEIDGVDLCFFGMHVQEYGSDCPP
                     PNQRRVYISYLYSVHFFRPKCLRTAVYHEILIGYLEYVKKLGYTTGHIWACPPSEGDDY
                     IFHCHPPDQKIPKPKRLQEWYKKMLDKAVSERIVHDYKDIFKQATEDRLTSAKELPYFE
                     GDFWPNVLEESIKELEQEEEERKREENTSNESTDVTKGDSKNAKKKNNKKTSKNKSSLS
                     RGNKKKPGMPNVSNDLSQKLYATMEKHKEVFFVIRLIAGPAANSLPPIVDPDPLIPCDL
                     MDGRDAFLTLARDKHLEFSSLRRAQWSTMCMLVELHTQSQD"
     regulatory      4324..4333
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             4567..4587
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     regulatory      4600..4609
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             4606..8709
                     /codon_start=1
                     /product="Cas9 (Csn1) endonuclease from the Streptococcus 
                     pyogenes Type II CRISPR/Cas system"
                     /label=Cas9
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="MDKKYSIGLAIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKK
                     NLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEES
                     FLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIK
                     FRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINAGGVDAKAILSARLSKSRRL
                     ENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQ
                     IGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVR
                     QQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLL
                     RKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARG
                     NSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEY
                     FTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFD
                     SVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEER
                     LKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFM
                     QLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGR
                     HKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLY
                     LYYLQNGRDMYVDQELDINRLSDYDVDAIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVP
                     SEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKH
                     VAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYL
                     NAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKT
                     EITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSK
                     ESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGI
                     TIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNE
                     LALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILAD
                     ANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVL
                     DATLIHQSITGLYETRIDLSQLGGD"
     CDS             8713..8739
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             8719..8745
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /note="This is a variant of the standard HA tag, which has 
                     sequence YPYDVPDYA. Some anti-HA antibodies may not 
                     recognize this variant."
                     /translation="YDVPDYASL"
     CDS             8758..8778
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             8785..8805
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             8845..8901
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             join(8902..8904,8905..8907,8908..9621)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(8902..8904,8905..8907,8908..9618)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(9208..9227)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     9555..9576
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     misc_feature    9644..10232
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     CDS             complement(10115..10126)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     promoter        10239..11450
                     /label=UbC promoter
                     /note="human ubiquitin C promoter"
     primer_bind     11287..11306
                     /label=hUBCpro-F
                     /note="Human Ubiquitin C (UbC) promoter, forward primer"
     protein_bind    complement(11463..11496)
                     /label=loxP511
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATAC) (Shaw et al., 2021). loxP511 sites are 
                     compatible with each other, but incompatible with wild-type
                     loxP sites."
     CDS             11503..12210
                     /codon_start=1
                     /product="reverse tetracycline transactivator 3"
                     /label=rtTA3
                     /note="activates expression from Tet-responsive promoters 
                     in the presence of doxycycline"
                     /translation="MSRLDKSKVINGALELLNGVGIEGLTTRKLAQKLGVEQPTLYWHV
                     KNKRALLDALPIEMLDRHHTHFCPLEGESWQDFLRNNAKSYRCALLSHRDGAKVHLGTR
                     PTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEEQEHQVAKEERETPTT
                     DSMPPLLRQAIELFDRQGAEPAFLFGLELIICGLEKQLKCESGGPTDALDDFDLDMLPA
                     DALDDFDLDMLPG"
     protein_bind    complement(12211..12244)
                     /label=loxP511
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATAC) (Shaw et al., 2021). loxP511 sites are 
                     compatible with each other, but incompatible with wild-type
                     loxP sites."
     misc_feature    12267..12839
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(12434..12451)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     12661..12680
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             12842..13867
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     CDS             12842..13867
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     polyA_signal    13993..14114
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(14030..14049)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     14084..14103
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     repeat_region   14877..14911
                     /label=piggyBac left (5') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
     primer_bind     complement(15402..15418)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(15402..15418)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(15415..15437)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    15426..15442
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(15450..15456,15457..15474,15475..15480))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    15495..15516
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(15633..15650)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(15804..16392)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(15884..15903)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 gaagatcctt tgatcttttc tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa
       61 gggattttgg tcatgagatt atcaaaaagg atcttcacct agatcctttt aaattaaaaa
      121 tgaagtttta aatcaatcta aagtatatat gagtaaactt ggtctgacag ttaccaatgc
      181 ttaatcagtg aggcacctat ctcagcgatc tgtctatttc gttcatccat agttgcctga
      241 ctccccgtcg tgtagataac tacgatacgg gagggcttac catctggccc cagtgctgca
      301 atgataccgc gagacccacg ctcaccggct ccagatttat cagcaataaa ccagccagcc
      361 ggaagggccg agcgcagaag tggtcctgca actttatccg cctccatcca gtctattaat
      421 tgttgccggg aagctagagt aagtagttcg ccagttaata gtttgcgcaa cgttgttgcc
      481 attgctacag gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt cagctccggt
      541 tcccaacgat caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc ggttagctcc
      601 ttcggtcctc cgatcgttgt cagaagtaag ttggccgcag tgttatcact catggttatg
      661 gcagcactgc ataattctct tactgtcatg ccatccgtaa gatgcttttc tgtgactggt
      721 gagtactcaa ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg ctcttgcccg
      781 gcgtcaatac gggataatac cgcgccacat agcagaactt taaaagtgct catcattgga
      841 aaacgttctt cggggcgaaa actctcaagg atcttaccgc tgttgagatc cagttcgatg
      901 taacccactc gtgcacccaa ctgatcttca gcatctttta ctttcaccag cgtttctggg
      961 tgagcaaaaa caggaaggca aaatgccgca aaaaagggaa taagggcgac acggaaatgt
     1021 tgaatactca tactcttcct ttttcaatat tattgaagca tttatcaggg ttattgtctc
     1081 atgagcggat acatatttga atgtatttag aaaaataaac aaataggggt tccgcgcaca
     1141 tttccccgaa aagtgccacc taaattgtaa gcgttaatat tttgttaaaa ttcgcgttaa
     1201 atttttgtta aatcagctca ttttttaacc aataggccga aatcggcaaa atcccttata
     1261 aatcaaaaga atagaccgag atagggttga gtgttgttcc agtttggaac aagagtccac
     1321 tattaaagaa cgtggactcc aacgtcaaag ggcgaaaaac cgtctatcag ggcgatggcc
     1381 cactacgtga accatcaccc taatcaagtt ttttggggtc gaggtgccgt aaagcactaa
     1441 atcggaaccc taaagggagc ccccgattta gagcttgacg gggaaagccg gcgaacgtgg
     1501 cgagaaagga agggaagaaa gcgaaaggag cgggcgctag ggcgctggca agtgtagcgg
     1561 tcacgctgcg cgtaaccacc acacccgccg cgcttaatgc gccgctacag ggcgcgtccc
     1621 attcgccatt caggctgcgc aactgttggg aagggcgatc ggtgcgggcc tcttcgctat
     1681 tacgccagct ggcgaaaggg ggatgtgctg caaggcgatt aagttgggta acgccagggt
     1741 tttcccagtc acgacgttgt aaaacgacgg ccagtgagcg cgcctcgttc attcacgttt
     1801 ttgaacccgt ggaggacggg cagactcgcg gtgcaaatgt gttttacagc gtgatggagc
     1861 agatgaagat gctcgacacg ctgcagaaca cgcagctaga ttaaccctag aaagataatc
     1921 atattgtgac gtacgttaaa gataatcatg tgtaaaattg acgcatgtgt tttatcggtc
     1981 tgtatatcga ggtttattta ttaatttgaa tagatattaa gttttattat atttacactt
     2041 acatactaat aataaattca acaaacaatt tatttatgtt tatttattta ttaaaaaaaa
     2101 caaaaactca aaatttcttc tataaagtaa caaaactttt atgagggaca gccccccccc
     2161 aaagccccca gggatgtaat tacgtccctc ccccgctagg gggcagcagc gagccgcccg
     2221 gggctccgct ccggtccggc gctccccccg catccccgag ccggcagcgt gcggggacag
     2281 cccgggcacg gggaaggtgg cacgggatcg ctttcctctg aacgcttctc gctgctcttt
     2341 gagcctgcag acacctgggg ggatacgggg aaaaggcctc cacggccaga ctagacgagt
     2401 ttactcccta tcagtgatag agaacgatgt cgagtttact ccctatcagt gatagagaac
     2461 gtatgtcgag tttactccct atcagtgata gagaacgtat gtcgagttta ctccctatca
     2521 gtgatagaga acgtatgtcg agtttatccc tatcagtgat agagaacgta tgtcgagttt
     2581 actccctatc agtgatagag aacgtatgtc gaggtaggcg tgtacggtgg gaggcctata
     2641 taagcagagc tcgtttagtg aaccgtcaga tcgcaccggt ggtaccgact cgaattccgc
     2701 ccgggcatga ttttcaaacc agaagaacta cgacaggcac tgatgccaac tttggaggca
     2761 ctttaccgtc aggatccaga atcccttccc tttcgtcaac ctgtggaccc tcagctttta
     2821 ggaatccctg attactttga tattgtgaag agccccatgg atctttctac cattaagagg
     2881 aagttagaca ctggacagta tcaggagccc tggcagtatg tcgatgatat ttggcttatg
     2941 ttcaataatg cctggttata taaccggaaa acatcacggg tatacaaata ctgctccaag
     3001 ctctctgagg tctttgaaca agaaattgac ccagtgatgc aaagccttgg atactgttgt
     3061 ggcagaaagt tggagttctc tccacagaca ctgtgttgct acggcaaaca gttgtgcaca
     3121 atacctcgtg atgccactta ttacagttac cagaacaggt atcatttctg tgagaagtgt
     3181 ttcaatgaga tccaagggga gagcgtttct ttgggggatg acccttccca gcctcaaact
     3241 acaataaata aagaacaatt ttccaagaga aaaaatgaca cactggatcc tgaactgttt
     3301 gttgaatgta cagagtgcgg aagaaagatg catcagatct gtgtccttca ccatgagatc
     3361 atctggcctg ctggattcgt ctgtgatggc tgtttaaaga aaagtgcacg aactaggaaa
     3421 gaaaataagt tttctgctaa aaggttgcca tctaccagac ttggcacctt tctagagaat
     3481 cgtgtgaatg actttctgag gcgacagaat caccctgagt caggagaggt cactgttaga
     3541 gtagttcatg cttctgacaa aaccgtggaa gtaaaaccag gcatgaaagc aaggtttgtg
     3601 gacagtggag agatggcaga atcctttcca taccgaacca aagccctctt tgcctttgaa
     3661 gaaattgatg gtgttgacct gtgcttcttt ggcatgcatg ttcaagagta tggctctgac
     3721 tgccctccac ccaaccagag gagagtatac atatcttacc tctatagtgt tcatttcttc
     3781 cgtcctaaat gcttgaggac tgcagtctat catgaaatcc taattggata tttagaatat
     3841 gtcaagaaat taggttacac aacagggcat atttgggcat gtccaccaag tgagggagat
     3901 gattatatct tccattgcca tcctcctgac cagaagatac ccaagcccaa gcgactgcag
     3961 gaatggtaca aaaaaatgct tgacaaggct gtatcagagc gtattgtcca tgactacaag
     4021 gatattttta aacaagctac tgaagataga ttaacaagtg caaaggaatt gccttatttc
     4081 gagggtgatt tctggcccaa tgttctggaa gaaagcatta aggaactgga acaggaggaa
     4141 gaagagagaa aacgagagga aaacaccagc aatgaaagca cagatgtgac caagggagac
     4201 agcaaaaatg ctaaaaagaa gaataataag aaaaccagca aaaataagag cagcctgagt
     4261 aggggcaaca agaagaaacc cgggatgccc aatgtatcta acgacctctc acagaaacta
     4321 tatgccacca tggagaagca taaagaggtc ttctttgtga tccgcctcat tgctggccct
     4381 gctgccaact ccctgcctcc cattgttgat cctgatcctc tcatcccctg cgatctgatg
     4441 gatggtcggg atgcgtttct cacgctggca agggacaagc acctggagtt ctcttcactc
     4501 cgaagagccc agtggtccac catgtgcatg ctggtggagc tgcacacgca gagccaggac
     4561 ggatccccca agaagaagag gaaggtggga tccagcgctg ccaccatgga caagaagtac
     4621 agcatcggcc tggccatcgg caccaactct gtaggctggg ccgtgatcac cgacgagtac
     4681 aaggtgccca gcaagaaatt caaggtgctg ggcaacaccg accggcacag catcaagaag
     4741 aacctgatcg gcgccctgct gttcgacagc ggagaaacag ccgaggccac ccggctgaag
     4801 agaaccgcca gaagaagata caccagacgg aagaaccgga tctgctatct gcaagagatc
     4861 ttcagcaacg agatggccaa ggtggacgac agcttcttcc acagactgga agagtccttc
     4921 ctggtggaag aggataagaa gcacgagcgg caccccatct tcggcaacat cgtggacgag
     4981 gtggcctacc acgagaagta ccccaccatc taccacctga gaaagaaact ggtggacagc
     5041 accgacaagg ccgacctgcg gctgatctat ctggccctgg cccacatgat caagttccgg
     5101 ggccacttcc tgatcgaggg cgacctgaac cccgacaaca gcgacgtgga caagctgttc
     5161 atccagctgg tgcagaccta caaccagctg ttcgaggaaa accccatcaa cgccggcggc
     5221 gtggacgcca aggccatcct gtctgccaga ctgagcaaga gcagacggct ggaaaatctg
     5281 atcgcccagc tgcccggcga gaagaagaat ggcctgttcg gcaacctgat tgccctgagc
     5341 ctgggcctga cccccaactt caagagcaac ttcgacctgg ccgaggatgc caaactgcag
     5401 ctgagcaagg acacctacga cgacgacctg gacaacctgc tggcccagat cggcgaccag
     5461 tacgccgacc tgtttctggc cgccaagaac ctgtccgacg ccatcctgct gagcgacatc
     5521 ctgagagtga acaccgagat caccaaggcc cccctgagcg cctctatgat caagagatac
     5581 gacgagcacc accaggacct gaccctgctg aaagctctcg tgcggcagca gctgcctgag
     5641 aagtacaaag agattttctt cgaccagagc aagaacggct acgccggcta catcgatggc
     5701 ggagccagcc aggaagagtt ctacaagttc atcaagccca tcctggaaaa gatggacggc
     5761 accgaggaac tgctcgtgaa gctgaacaga gaggacctgc tgcggaagca gcggaccttc
     5821 gacaacggca gcatccccca ccagatccac ctgggagagc tgcacgccat tctgcggcgg
     5881 caggaagatt tttacccatt cctgaaggac aaccgggaaa agatcgagaa gatcctgacc
     5941 ttccgcatcc cctactacgt gggccctctg gccaggggaa acagcagatt cgcctggatg
     6001 accagaaaga gcgaggaaac catcaccccc tggaacttcg aggaagtggt ggacaagggc
     6061 gccagcgccc agagcttcat cgagcggatg accaacttcg ataagaacct gcccaacgag
     6121 aaggtgctgc ccaagcacag cctgctgtac gagtacttca ccgtgtacaa cgagctgacc
     6181 aaagtgaaat acgtgaccga gggaatgaga aagcccgcct tcctgagcgg cgagcagaaa
     6241 aaagccatcg tggacctgct gttcaagacc aaccggaaag tgaccgtgaa gcagctgaaa
     6301 gaggactact tcaagaaaat cgagtgcttc gactccgtgg aaatctccgg cgtggaagat
     6361 cggttcaacg cctccctggg cacataccac gatctgctga aaattatcaa ggacaaggac
     6421 ttcctggaca atgaggaaaa cgaggacatt ctggaagata tcgtgctgac cctgacactg
     6481 tttgaggaca gagagatgat cgaggaacgg ctgaaaacct atgcccacct gttcgacgac
     6541 aaagtgatga agcagctgaa gcggcggaga tacaccggct ggggcaggct gagccggaag
     6601 ctgatcaacg gcatccggga caagcagtcc ggcaagacaa tcctggattt cctgaagtcc
     6661 gacggcttcg ccaacagaaa cttcatgcag ctgatccacg acgacagcct gacctttaaa
     6721 gaggacatcc agaaagccca ggtgtccggc cagggcgata gcctgcacga gcacattgcc
     6781 aatctggccg gcagccccgc cattaagaag ggcatcctgc agacagtgaa ggtggtggac
     6841 gagctcgtga aagtgatggg ccggcacaag cccgagaaca tcgtgatcga aatggccaga
     6901 gagaaccaga ccacccagaa gggacagaag aacagccgcg agagaatgaa gcggatcgaa
     6961 gagggcatca aagagctggg cagccagatc ctgaaagaac accccgtgga aaacacccag
     7021 ctgcagaacg agaagctgta cctgtactac ctgcagaatg ggcgggatat gtacgtggac
     7081 caggaactgg acatcaaccg gctgtccgac tacgatgtgg acgctatcgt gcctcagagc
     7141 tttctgaagg acgactccat cgataacaaa gtgctgactc ggagcgacaa gaaccggggc
     7201 aagagcgaca acgtgccctc cgaagaggtc gtgaagaaga tgaagaacta ctggcgccag
     7261 ctgctgaatg ccaagctgat tacccagagg aagttcgaca atctgaccaa ggccgagaga
     7321 ggcggcctga gcgaactgga taaggccggc ttcatcaaga gacagctggt ggaaacccgg
     7381 cagatcacaa agcacgtggc acagatcctg gactcccgga tgaacactaa gtacgacgag
     7441 aacgacaaac tgatccggga agtgaaagtg atcaccctga agtccaagct ggtgtccgat
     7501 ttccggaagg atttccagtt ttacaaagtg cgcgagatca acaactacca ccacgcccac
     7561 gacgcctacc tgaacgccgt cgtgggaacc gccctgatca aaaagtaccc taagctggaa
     7621 agcgagttcg tgtacggcga ctacaaggtg tacgacgtgc ggaagatgat cgccaagagc
     7681 gagcaggaaa tcggcaaggc taccgccaag tacttcttct acagcaacat catgaacttt
     7741 ttcaagaccg agattaccct ggccaacggc gagatccgga agcggcctct gatcgagaca
     7801 aacggcgaaa caggcgagat cgtgtgggat aagggccggg actttgccac cgtgcggaaa
     7861 gtgctgtcta tgccccaagt gaatatcgtg aaaaagaccg aggtgcagac aggcggcttc
     7921 agcaaagagt ctatcctgcc caagaggaac agcgacaagc tgatcgccag aaagaaggac
     7981 tgggacccta agaagtacgg cggcttcgac agccccaccg tggcctattc tgtgctggtg
     8041 gtggccaaag tggaaaaggg caagtccaag aaactgaaga gtgtgaaaga gctgctgggg
     8101 atcaccatca tggaaagaag cagcttcgag aagaatccca tcgactttct ggaagccaag
     8161 ggctacaaag aagtgaaaaa ggacctgatc atcaagctgc ctaagtactc cctgttcgag
     8221 ctggaaaacg gccggaagag aatgctggcc tctgccggcg aactgcagaa gggaaacgaa
     8281 ctggccctgc cctccaaata tgtgaacttc ctgtacctgg ccagccacta tgagaagctg
     8341 aagggctccc ccgaggataa tgagcagaaa cagctgtttg tggaacagca caaacactac
     8401 ctggacgaga tcatcgagca gatcagcgag ttctccaaga gagtgatcct ggccgacgct
     8461 aatctggaca aggtgctgag cgcctacaac aagcacagag acaagcctat cagagagcag
     8521 gccgagaata tcatccacct gtttaccctg accaatctgg gagcccctgc cgccttcaag
     8581 tactttgaca ccaccatcga ccggaagagg tacaccagca ccaaagaggt gctggacgcc
     8641 accctgatcc accagagcat caccggcctg tacgagacac ggatcgacct gtctcagctg
     8701 ggaggcgacg cctatcccta tgacgtgccc gattatgcca gcctgggcag cggctccccc
     8761 aagaaaaaac gcaaggtgga agatcctaag aaaaagcgga aagtggacgg cattggtagt
     8821 gggagcaacg gcagcagcgg atccgctact aacttcagcc tgctgaagca ggctggagac
     8881 gtggaggaga accctggacc tatggtgagc aagggcgagg agctgttcac cggggtggtg
     8941 cccatcctgg tcgagctgga cggcgacgta aacggccaca agttcagcgt gtccggcgag
     9001 ggcgagggcg atgccaccta cggcaagctg accctgaagt tcatctgcac caccggcaag
     9061 ctgcccgtgc cctggcccac cctcgtgacc accctgacct acggcgtgca gtgcttcagc
     9121 cgctaccccg accacatgaa gcagcacgac ttcttcaagt ccgccatgcc cgaaggctac
     9181 gtccaggagc gcaccatctt cttcaaggac gacggcaact acaagacccg cgccgaggtg
     9241 aagttcgagg gcgacaccct ggtgaaccgc atcgagctga agggcatcga cttcaaggag
     9301 gacggcaaca tcctggggca caagctggag tacaactaca acagccacaa cgtctatatc
     9361 atggccgaca agcagaagaa cggcatcaag gtgaacttca agatccgcca caacatcgag
     9421 gacggcagcg tgcagctcgc cgaccactac cagcagaaca cccccatcgg cgacggcccc
     9481 gtgctgctgc ccgacaacca ctacctgagc acccagtccg ccctgagcaa agaccccaac
     9541 gagaagcgcg atcacatggt cctgctggag ttcgtgaccg ccgccgggat cactctcggc
     9601 atggacgagc tgtacaagta atgtacaagg cggccgcgtc gacaatcaac ctctggatta
     9661 caaaatttgt gaaagattga ctggtattct taactatgtt gctcctttta cgctatgtgg
     9721 atacgctgct ttaatgcctt tgtatcatgc tattgcttcc cgtatggctt tcattttctc
     9781 ctccttgtat aaatcctggt tgctgtctct ttatgaggag ttgtggcccg ttgtcaggca
     9841 acgtggcgtg gtgtgcactg tgtttgctga cgcaaccccc actggttggg gcattgccac
     9901 cacctgtcag ctcctttccg ggactttcgc tttccccctc cctattgcca cggcggaact
     9961 catcgccgcc tgccttgccc gctgctggac aggggctcgg ctgttgggca ctgacaattc
    10021 cgtggtgttg tcggggaagc tgacgtcctt tccatggctg ctcgcctgtg ttgccacctg
    10081 gattctgcgc gggacgtcct tctgctacgt cccttcggcc ctcaatccag cggaccttcc
    10141 ttcccgcggc ctgctgccgg ctctgcggcc tcttccgcgt cttcgccttc gccctcagac
    10201 gagtcggatc tccctttggg ccgcctcccc gcacgcgtgg cctccgcgcc gggttttggc
    10261 gcctcccgcg ggcgcccccc tcctcacggc gagcgctgcc acgtcagacg aagggcgcag
    10321 cgagcgtcct gatccttccg cccggacgct caggacagcg gcccgctgct cataagactc
    10381 ggccttagaa ccccagtatc agcagaagga cattttagga cgggacttgg gtgactctag
    10441 ggcactggtt ttctttccag agagcggaac aggcgaggaa aagtagtccc ttctcggcga
    10501 ttctgcggag ggatctccgt ggggcggtga acgccgatga ttatataagg acgcgccggg
    10561 tgtggcacag ctagttccgt cgcagccggg atttgggtcg cggttcttgt ttgtggatcg
    10621 ctgtgatcgt cacttggtga gtagcgggct gctgggctgg ccggggcttt cgtggccgcc
    10681 gggccgctcg gtgggacgga agcgtgtgga gagaccgcca agggctgtag tctgggtccg
    10741 cgagcaaggt tgccctgaac tgggggttgg ggggagcgca gcaaaatggc ggctgttccc
    10801 gagtcttgaa tggaagacgc ttgtgaggcg ggctgtgagg tcgttgaaac aaggtggggg
    10861 gcatggtggg cggcaagaac ccaaggtctt gaggccttcg ctaatgcggg aaagctctta
    10921 ttcgggtgag atgggctggg gcaccatctg gggaccctga cgtgaagttt gtcactgact
    10981 ggagaactcg gtttgtcgtc tgttgcgggg gcggcagtta tggcggtgcc gttgggcagt
    11041 gcacccgtac ctttgggagc gcgcgccctc gtcgtgtcgt gacgtcaccc gttctgttgg
    11101 cttataatgc agggtggggc cacctgccgg taggtgtgcg gtaggctttt ctccgtcgca
    11161 ggacgcaggg ttcgggccta gggtaggctc tcctgaatcg acaggcgccg gacctctggt
    11221 gaggggaggg ataagtgagg cgtcagtttc tttggtcggt tttatgtacc tatcttctta
    11281 agtagctgaa gctccggttt tgaactatgc gctcggggtt ggcgagtgtg ttttgtgaag
    11341 ttttttaggc accttttgaa atgtaatcat ttgggtcaat atgtaatttt cagtgttaga
    11401 ctagtaaatt gtccgctaaa ttctggccgt ttttggcttt tttgttagac gctagcggat
    11461 ccataacttc gtatagtata cattatacga agttatgcca ccatgtctag gctggacaag
    11521 agcaaagtca taaacggagc tctggaatta ctcaatggtg tcggtatcga aggcctgacg
    11581 acaaggaaac tcgctcaaaa gctgggagtt gagcagccta ccctgtactg gcacgtgaag
    11641 aacaagcggg ccctgctcga tgccctgcca atcgagatgc tggacaggca tcatacccac
    11701 ttctgccccc tggaaggcga gtcatggcaa gactttctgc ggaacaacgc caagtcatac
    11761 cgctgtgctc tcctctcaca tcgcgacggg gctaaagtgc atctcggcac ccgcccaaca
    11821 gagaaacagt acgaaaccct ggaaaatcag ctcgcgttcc tgtgtcagca aggcttctcc
    11881 ctggagaacg cactgtacgc tctgtccgcc gtgggccact ttacactggg ctgcgtattg
    11941 gaggaacagg agcatcaagt agcaaaagag gaaagagaga cacctaccac cgattctatg
    12001 cccccacttc tgagacaagc aattgagctg ttcgaccggc agggagccga acctgccttc
    12061 cttttcggcc tggaactaat catatgtggc ctggagaaac agctaaagtg cgaaagcggc
    12121 gggccgaccg acgcccttga cgattttgac ttagacatgc tcccagccga tgcccttgac
    12181 gattttgacc ttgacatgct ccccgggtaa ataacttcgt atagtataca ttatacgaag
    12241 ttatggatct gcggccgcaa attccgcccc tctccctccc ccccccctaa cgttactggc
    12301 cgaagccgct tggaataagg ccggtgtgcg tttgtctata tgttattttc caccatattg
    12361 ccgtcttttg gcaatgtgag ggcccggaaa cctggccctg tcttcttgac gagcattcct
    12421 aggggtcttt cccctctcgc caaaggaatg caaggtctgt tgaatgtcgt gaaggaagca
    12481 gttcctctgg aagcttcttg aagacaaaca acgtctgtag cgaccctttg caggcagcgg
    12541 aaccccccac ctggcgacag gtgcctctgc ggccaaaagc cacgtgtata agatacacct
    12601 gcaaaggcgg cacaacccca gtgccacgtt gtgagttgga tagttgtgga aagagtcaaa
    12661 tggctctcct caagcgtatt caacaagggg ctgaaggatg cccagaaggt accccattgt
    12721 atgggatctg atctggggcc tcggtgcaca tgctttacat gtgtttagtc gaggttaaaa
    12781 aaacgtctag gccccccgaa ccacggggac gtggttttcc tttgaaaaac acgataatac
    12841 catgaaaaag cctgaactca ccgcgacgtc tgtcgagaag tttctgatcg aaaagttcga
    12901 cagcgtctcc gacctgatgc agctctcgga gggcgaagaa tctcgtgctt tcagcttcga
    12961 tgtaggaggg cgtggatatg tcctgcgggt aaatagctgc gccgatggtt tctacaaaga
    13021 tcgttatgtt tatcggcact ttgcatcggc cgcgctcccg attccggaag tgcttgacat
    13081 tggggaattc agcgagagcc tgacctattg catctcccgc cgtgcacagg gtgtcacgtt
    13141 gcaagacctg cctgaaaccg aactgcccgc tgttctgcag ccggtcgcgg aggccatgga
    13201 tgcgatcgct gcggccgatc ttagccagac gagcgggttc ggcccattcg gaccgcaagg
    13261 aatcggtcaa tacactacat ggcgtgattt catatgcgcg attgctgatc cccatgtgta
    13321 tcactggcaa actgtgatgg acgacaccgt cagtgcgtcc gtcgcgcagg ctctcgatga
    13381 gctgatgctt tgggccgagg actgccccga agtccggcac ctcgtgcacg cggatttcgg
    13441 ctccaacaat gtcctgacgg acaatggccg cataacagcg gtcattgact ggagcgaggc
    13501 gatgttcggg gattcccaat acgaggtcgc caacatcttc ttctggaggc cgtggttggc
    13561 ttgtatggag cagcagacgc gctacttcga gcggaggcat ccggagcttg caggatcgcc
    13621 gcggctccgg gcgtatatgc tccgcattgg tcttgaccaa ctctatcaga gcttggttga
    13681 cggcaatttc gatgatgcag cttgggcgca gggtcgatgc gacgcaatcg tccgatccgg
    13741 agccgggact gtcgggcgta cacaaatcgc ccgcagaagc gcggccgtct ggaccgatgg
    13801 ctgtgtagaa gtactcgccg atagtggaaa ccgacgcccc agcactcgtc cgagggcaaa
    13861 ggaataggtc gacaatcaac ctctggatta caaaatttgt gaaagattga ctggtattct
    13921 taactatgtt gctcctttta cgctatgtgg atacgctgct ttaatgcctt tgtatcatgc
    13981 gttaactaac taaacttgtt tattgcagct tataatggtt acaaataaag caatagcatc
    14041 acaaatttca caaataaagc atttttttca ctgcattcta gttgtggttt gtccaaactc
    14101 atcaatgtat cttatcatgt ctggaattga ctcaaatgat gtcaattagt ctatcagaag
    14161 ctcatctggt ctcccttccg ggggacaaga catccctgtt taatatttaa acagcagtgt
    14221 tcccaaactg ggttcttata tcccttgctc tggtcaacca ggttgcaggg tttcctgtcc
    14281 tcacaggaac gaagtcccta aagaaacagt ggcagccagg tttagccccg gaattgactg
    14341 gattcctttt ttagggccca ttggtatggc tttttccccg tatcccccca ggtgtctgca
    14401 ggctcaaaga gcagcgagaa gcgttcagag gaaagcgatc ccgtgccacc ttccccgtgc
    14461 ccgggctgtc cccgcacgct gccggctcgg ggatgcgggg ggagcgccgg accggagcgg
    14521 agccccgggc ggctcgctgc tgccccctag cgggggaggg acgtaattac atccctgggg
    14581 gctttggggg ggggctgtcc ctgatatcta taacaagaaa atatatatat aataagttat
    14641 cacgtaagta gaacatgaaa taacaatata attatcgtat gagttaaatc ttaaaagtca
    14701 cgtaaaagat aatcatgcgt cattttgact cacgcggtcg ttatagttca aaatcagtga
    14761 cacttaccgc attgacaagc acgcctcacg ggagctccaa gcggcgactg agatgtccta
    14821 aatgcacagc gacggattcg cgctatttag aaagagagag caatatttca agaatgcatg
    14881 cgtcaatttt acgcagacta tctttctagg gttaatctag ctgcatcagg atcatatcgt
    14941 cgggtctttt ttccggctca gtcatcgccc aagctggcgc tatctgggca tcggggagga
    15001 agaagcccgt gccttttccc gcgaggttga agcggcatgg aaagagtttg ccgaggatga
    15061 ctgctgctgc attgacgttg agcgaaaacg cacgtttacc atgatgattc gggaaggtgt
    15121 ggccatgcac gcctttaacg gtgaactgtt cgttcaggcc acctgggata ccagttcgtc
    15181 gcggcttttc cggacacagt tccggatggt cagcccgaag cgcatcagca acccgaacaa
    15241 taccggcgac agccggaact gccgtgccgg tgtgcagatt aatgacagcg gtgcggcgct
    15301 gggatattac gtcagcgagg acgggtatcc tggctggatg ccgcagaaat ggacatggat
    15361 accccgtgag ttacccggcg ggcgcgcttg gcgtaatcat ggtcatagct gtttcctgtg
    15421 tgaaattgtt atccgctcac aattccacac aacatacgag ccggaagcat aaagtgtaaa
    15481 gcctggggtg cctaatgagt gagctaactc acattaattg cgttgcgctc actgcccgct
    15541 ttccagtcgg gaaacctgtc gtgccagctg cattaatgaa tcggccaacg cgcggggaga
    15601 ggcggtttgc gtattgggcg ctattccgct tcctcgctca ctgactcgct gcgctcggtc
    15661 gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg taatacggtt atccacagaa
    15721 tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt
    15781 aaaaaggccg cgttgctggc gtttttccat aggctccgcc cccctgacga gcatcacaaa
    15841 aatcgacgct caagtcagag gtggcgaaac ccgacaggac tataaagata ccaggcgttt
    15901 ccccctggaa gctccctcgt gcgctctcct gttccgaccc tgccgcttac cggatacctg
    15961 tccgcctttc tcccttcggg aagcgtggcg ctttctcata gctcacgctg taggtatctc
    16021 agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc
    16081 gaccgctgcg ccttatccgg taactatcgt cttgagtcca acccggtaag acacgactta
    16141 tcgccactgg cagcagccac tggtaacagg attagcagag cgaggtatgt aggcggtgct
    16201 acagagttct tgaagtggtg gcctaactac ggctacacta gaagaacagt atttggtatc
    16261 tgcgctctgc tgaagccagt taccttcgga aaaagagttg gtagctcttg atccggcaaa
    16321 caaaccaccg ctggtagcgg tggttttttt gtttgcaagc agcagattac gcgcagaaaa
    16381 aaaggatctc aa
//
