LOCUS       sequence-458113-        9112 bp    DNA     circular SYN 15-APR-2025
DEFINITION  Bacterial expression vector of MBD4 C-terminal tagged to the 
            intein..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9112)
  TITLE     MBD2 modulates heterchromatin architecture and composition
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 9112)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pTYB1-MBD4 pc4785
FEATURES             Location/Qualifiers
     source          1..9112
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      1..589
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     490..509
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     743..760
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     complement(782..801)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     942..964
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     CDS             complement(958..1149)
                     /codon_start=1
                     /gene="rop"
                     /product="Rop protein, which maintains plasmids at low copy
                     number"
                     /label=rop
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGENL"
     CDS             complement(1706..2788)
                     /codon_start=1
                     /gene="lacI"
                     /product="lac repressor"
                     /label=lacI
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
                     /translation="MKPVTLYDVAEYAGVSYQTVSRVVNQASHVSAKTREKVEAAMAEL
                     NYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKSRADQLGASVVVSMVERSGV
                     EACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACTNVPALFLDVSDQTPINSIIFSH
                     EDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGWHKYLTRNQIQPIAEREGDWSA
                     MSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITESGLRVGADISVVGYDDTEDSSC
                     YIPPLTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQLLPVSLVKRKTTLAPNTQTASPR
                     ALADSLMQLARQVSRLESGQ"
     primer_bind     2750..2769
                     /label=LacI-R
                     /note="LacI, reverse primer"
     promoter        complement(2789..2866)
                     /gene="lacI"
                     /label=lacI promoter
     terminator      3019..3105
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      3202..3288
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      3385..3471
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      3568..3654
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      3751..3837
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     3972..3991
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        3972..3990
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    3991..4015
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     RBS             4030..4052
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             5070..5081
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     CDS             5746..6564
                     /codon_start=1
                     /gene="Saccharomyces cerevisiae VMA1"
                     /product="5' region of the intein from the yeast Vma1 
                     subunit of the vacuolar ATPase (Chong et al., 1998)"
                     /label=Sce VMA intein 5' region
                     /translation="CFAKGTNVLMADGSIECIENIEVGNKVMGKDGRPREVIKLPRGRE
                     TMYSVVQKSQHRAHKSDSSREVPELLKFTCNATHELVVRTPRSVRRLSRTIKGVEYFEV
                     ITFEMGQKKAPDGRIVELVKEVSKSYPISEGPERANELVESYRKASNKAYFEWTIEARD
                     LSLLGSHVRKATYQTYAPILYENDHFFDYMQKSKFHLTIEGPKVLAYLLGLWIGDGLSD
                     RATFSVDSRDTSLMERVTEYAEKLNLCAEYKDRKEPQVAKTVNLYSKVVRG"
     CDS             7020..7031
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     CDS             7141..7296
                     /codon_start=1
                     /gene="Bacillus circulans chiA"
                     /product="chitin binding domain from chitinase A1 (Watanabe
                     et al., 1994)"
                     /label=CBD
                     /translation="TTNPGVSAWQVNTAYTAGQLVTYNGKTYKCLQPHTSLAGWEPSNV
                     PALWQLQ"
     primer_bind     complement(7361..7379)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      7375..7422
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     promoter        7482..7586
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(7587..7655,7656..8447)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(7805..7824)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      complement(8489..9002)
                     /direction=LEFT
                     /label=M13 ori
                     /note="M13 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(8684..8705)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     8896..8915
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
ORIGIN
        1 ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc
       61 agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt
      121 cagcagagcg cagataccaa atactgtcct tctagtgtag ccgtagttag gccaccactt
      181 caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc
      241 tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa
      301 ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac
      361 ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg
      421 gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga
      481 gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact
      541 tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa
      601 cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc
      661 gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg
      721 ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagctatgg tgcactctca
      781 gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga
      841 ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg
      901 tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca
      961 gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg
     1021 gtcgtgcagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc
     1081 cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg
     1141 tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat
     1201 gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga
     1261 acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca
     1321 gggtcaatgc cagccgaacg ccagcaagac gtagcccagc gcgtcggccg ccatgccggc
     1381 gataatggcc tgcttctcgc cgaaacgttt ggtggcggga ccagtgacga aggcttgagc
     1441 gagggcgtgc aagattccga ataccgcaag cgacaggccg atcatcgtcg cgctccagcg
     1501 aaagcggtcc tcgccgaaaa tgacccagag cgctgccggc acctgtccta cgagttgcat
     1561 gataaagaag acagtcataa gtgcggcgac gatagtcatg ccccgcgccc accggaagga
     1621 gctgactggg ttgaaggctc tcaagggcat cggtcgagat cccggtgcct aatgagtgag
     1681 ctaacttaca ttaattgcgt tgcgctcact gcccgctttc cagtcgggaa acctgtcgtg
     1741 ccagctgcat taatgaatcg gccaacgcgc ggggagaggc ggtttgcgta ttgggcgcca
     1801 gggtggtttt tcttttcacc agtgagacgg gcaacagctg attgcccttc accgcctggc
     1861 cctgagagag ttgcagcaag cggtccacgc tggtttgccc cagcaggcga aaatcctgtt
     1921 tgatggtggt taacggcggg atataacatg agctgtcttc ggtatcgtcg tatcccacta
     1981 ccgagatatc cgcaccaacg cgcagcccgg actcggtaat ggcgcgcatt gcgcccagcg
     2041 ccatctgatc gttggcaacc agcatcgcag tgggaacgat gccctcattc agcatttgca
     2101 tggtttgttg aaaaccggac atggcactcc agtcgccttc ccgttccgct atcggctgaa
     2161 tttgattgcg agtgagatat ttatgccagc cagccagacg cagacgcgcc gagacagaac
     2221 ttaatgggcc cgctaacagc gcgatttgct ggtgacccaa tgcgaccaga tgctccacgc
     2281 ccagtcgcgt accgtcttca tgggagaaaa taatactgtt gatgggtgtc tggtcagaga
     2341 catcaagaaa taacgccgga acattagtgc aggcagcttc cacagcaatg gcatcctggt
     2401 catccagcgg atagttaatg atcagcccac tgacgcgttg cgcgagaaga ttgtgcaccg
     2461 ccgctttaca ggcttcgacg ccgcttcgtt ctaccatcga caccaccacg ctggcaccca
     2521 gttgatcggc gcgagattta atcgccgcga caatttgcga cggcgcgtgc agggccagac
     2581 tggaggtggc aacgccaatc agcaacgact gtttgcccgc cagttgttgt gccacgcggt
     2641 tgggaatgta attcagctcc gccatcgccg cttccacttt ttcccgcgtt ttcgcagaaa
     2701 cgtggctggc ctggttcacc acgcgggaaa cggtctgata agagacaccg gcatactctg
     2761 cgacatcgta taacgttact ggtttcacat tcaccaccct gaattgactc tcttccgggc
     2821 gctatcatgc cataccgcga aaggttttgc gccattcgat ggtgtccggg atctcgacgc
     2881 tctcccttat gcgactcctg cattaggaag cagcccagta gtaggttgag gccgttgagc
     2941 accgccgccg caaggaatgg tgcatgccgg catgccgccc tttcgtcttc aagaattaat
     3001 tcccaattcc ccaggcatca aataaaacga aaggctcagt cgaaagactg ggcctttcgt
     3061 tttatctgtt gtttgtcggt gaacgctctc ctgagtagga caaatccgcc gggagcggat
     3121 ttgaacgttg cgaagcaacg gcccggaggg tggcgggcag gacgcccgcc ataaactgcc
     3181 aggaattaat tccccaggca tcaaataaaa cgaaaggctc agtcgaaaga ctgggccttt
     3241 cgttttatct gttgtttgtc ggtgaacgct ctcctgagta ggacaaatcc gccgggagcg
     3301 gatttgaacg ttgcgaagca acggcccgga gggtggcggg caggacgccc gccataaact
     3361 gccaggaatt aattccccag gcatcaaata aaacgaaagg ctcagtcgaa agactgggcc
     3421 tttcgtttta tctgttgttt gtcggtgaac gctctcctga gtaggacaaa tccgccggga
     3481 gcggatttga acgttgcgaa gcaacggccc ggagggtggc gggcaggacg cccgccataa
     3541 actgccagga attaattccc caggcatcaa ataaaacgaa aggctcagtc gaaagactgg
     3601 gcctttcgtt ttatctgttg tttgtcggtg aacgctctcc tgagtaggac aaatccgccg
     3661 ggagcggatt tgaacgttgc gaagcaacgg cccggagggt ggcgggcagg acgcccgcca
     3721 taaactgcca ggaattaatt ccccaggcat caaataaaac gaaaggctca gtcgaaagac
     3781 tgggcctttc gttttatctg ttgtttgtcg gtgaacgctc tcctgagtag gacaaatccg
     3841 ccgggagcgg atttgaacgt tgcgaagcaa cggcccggag ggtggcgggc aggacgcccg
     3901 ccataaactg ccaggaattg gggatcggaa ttaattcccg gtttaaaccg gggatctcga
     3961 tcccgcgaaa ttaatacgac tcactatagg ggaattgtga gcggataaca attcccctct
     4021 agaaataatt ttgtttaact ttaagaagga gatatacata tgatggagag cccaaacctt
     4081 ggggacaaca gagtccgtgg agagagccta gttccagacc cgccttggga tcgctgcaag
     4141 gaagatattg ctgttggact gggaggagtg ggagaagatg gaaaggacct ggtgataagc
     4201 agtgagcgca gctcccttct ccaagagccc actgcttcta ctctgtctag tactacagcg
     4261 acagaaggcc acaagcctgt cccgtgtgga tgggaaagag ttgtgaagca aaggttatct
     4321 gggaaaactg caggaaaatt tgatgtatac tttatcagcc cacaaggatt gaagttcaga
     4381 tcaaaacgtt cacttgctaa ttatcttctc aaaaatgggg agacttttct taagcctgaa
     4441 gattttaatt ttactgtact gccgaaaggg agcatcaatc ccggttataa acaccaaagt
     4501 ttggcagctc tgacttccct gcagccaaat gaaactgacg tttcaaagca gaacctcaag
     4561 acacgaagca agtggaaaac agatgtgttg cctctgccca gtggtacttc agagtcgcca
     4621 gaaagcagcg gactgtctaa ctctaactcg gcttgcttgc tattgagaga acatagggac
     4681 attcaggatg ttgactctga gaagaggaga aagtccaaaa gaaaggtgac tgttttgaaa
     4741 ggaactgcaa gtcagaaaac caaacaaaag tgcaggaaga gtctcttaga gtctactcaa
     4801 agaaacagaa aaagagcatc tgtggttcag aaggtgggtg ctgatcgcga gctggtgcca
     4861 caggaaagtc aactcaacag aaccctctgc cctgcagatg cctgtgcaag ggagactgtt
     4921 ggcctggctg gggaagaaaa atcaccaagc ccaggactgg atctttgttt catacaagta
     4981 acttctggca ccacaaacaa attccattca actgaagcag caggtgaagc aaatcgtgag
     5041 cagacttttt tagaatcaga ggaaatcaga tcgaagggag acagaaaggg ggaggcacat
     5101 ttgcatactg gtgttttaca ggatggctct gaaatgccca gctgctcaca agccaagaaa
     5161 cactttactt ctgagacatt tcaagaagac agcatcccac ggacacaagt agaaaaaagg
     5221 aaaacaagcc tgtatttttc cagcaagtac aacaaagaag ctcttagccc cccaagacgc
     5281 aaatccttca agaaatggac ccctcctcgg tcacctttta atcttgttca agaaatactt
     5341 ttccatgacc catggaagct cctcatcgcg actatatttc tcaatcggac ctcaggcaag
     5401 atggccatcc ctgtgctgtg ggagtttcta gagaagtacc cttcagctga agtggcccga
     5461 gctgccgact ggagggacgt gtcggagctt ctcaagcctc ttggtctcta cgatctccgt
     5521 gcaaaaacca ttatcaagtt ctcagatgaa tatctgacaa agcagtggag gtatccgatt
     5581 gagcttcatg gcattggtaa atatggcaac gactcctacc ggatcttttg tgtcaatgaa
     5641 tggaagcagg tgcaccctga agaccacaag ttaaataaat accatgactg gctttgggag
     5701 aatcatgaaa aattaagtct atctgaattc ctcgagggct cttcctgctt tgccaagggt
     5761 accaatgttt taatggcgga tgggtctatt gaatgtattg aaaacattga ggttggtaat
     5821 aaggtcatgg gtaaagatgg cagacctcgt gaggtaatta aattgcccag aggaagagaa
     5881 actatgtaca gcgtcgtgca gaaaagtcag cacagagccc acaaaagtga ctcaagtcgt
     5941 gaagtgccag aattactcaa gtttacgtgt aatgcgaccc atgagttggt tgttagaaca
     6001 cctcgtagtg tccgccgttt gtctcgtacc attaagggtg tcgaatattt tgaagttatt
     6061 acttttgaga tgggccaaaa gaaagccccc gacggtagaa ttgttgagct tgtcaaggaa
     6121 gtttcaaaga gctacccaat atctgagggg cctgagagag ccaacgaatt agtagaatcc
     6181 tatagaaagg cttcaaataa agcttatttt gagtggacta ttgaggccag agatctttct
     6241 ctgttgggtt cccatgttcg taaagctacc taccagactt acgctccaat tctttatgag
     6301 aatgaccact ttttcgacta catgcaaaaa agtaagtttc atctcaccat tgaaggtcca
     6361 aaagtacttg cttatttact tggtttatgg attggtgatg gattgtctga cagggcaact
     6421 ttttcggttg attccagaga tacttctttg atggaacgtg ttactgaata tgctgaaaag
     6481 ttgaatttgt gcgccgagta taaggacaga aaagaaccac aagttgccaa aactgttaat
     6541 ttgtactcta aagttgtcag aggtaatggt attcgcaata atcttaatac tgagaatcca
     6601 ttatgggacg ctattgttgg cttaggattc ttgaaggacg gtgtcaaaaa tattccttct
     6661 ttcttgtcta cggacaatat cggtactcgt gaaacatttc ttgctggtct aattgattct
     6721 gatggctatg ttactgatga gcatggtatt aaagcaacaa taaagacaat tcatacttct
     6781 gtcagagatg gtttggtttc ccttgctcgt tctttaggct tagtagtctc ggttaacgca
     6841 gaacctgcta aggttgacat gaatgtcacc aaacataaaa ttagttatgc tatttatatg
     6901 tctggtggag atgttttgct taacgttctt tcgaagtgtg ccggctctaa aaaattcagg
     6961 cctgctcccg ccgctgcttt tgcacgtgag tgccgcggat tttatttcga gttacaagaa
     7021 ttgaaggaag acgattatta tgggattact ttatctgatg attctgatca tcagtttttg
     7081 cttggatccc aggttgtcgt ccatgcatgc ggtggcctga ccggtctgaa ctcaggcctc
     7141 acgacaaatc ctggtgtatc cgcttggcag gtcaacacag cttatactgc gggacaattg
     7201 gtcacatata acggcaagac gtataaatgt ttgcagcccc acacctcctt ggcaggatgg
     7261 gaaccatcca acgttcctgc cttgtggcag cttcaatgac tgcaggaagg ggatccggct
     7321 gctaacaaag cccgaaagga agctgagttg gctgctgcca ccgctgagca ataactagca
     7381 taaccccttg gggcctctaa acgggtcttg aggggttttt tgctgaaagg aggaactata
     7441 tccggataac tacgtcaggt ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt
     7501 tatttttcta aatacattca aatatgtatc cgctcatgag acaataaccc tgataaatgc
     7561 ttcaataata ttgaaaaagg aagagtatga gtattcaaca tttccgtgtc gcccttattc
     7621 ccttttttgc ggcattttgc cttcctgttt ttgctcaccc agaaacgctg gtgaaagtaa
     7681 aagatgctga agatcagttg ggtgcacgag tgggttacat cgaactggat ctcaacagcg
     7741 gtaagatcct tgagagtttt cgccccgaag aacgtttccc aatgatgagc acttttaaag
     7801 ttctgctatg tggcgcggta ttatcccgtg ttgacgccgg gcaagagcaa ctcggtcgcc
     7861 gcatacacta ttctcagaat gacttggttg agtactcacc agtcacagaa aagcatctta
     7921 cggatggcat gacagtaaga gaattatgca gtgctgccat aaccatgagt gataacactg
     7981 cggccaactt acttctgaca acgatcggag gaccgaagga gctaaccgct tttttgcaca
     8041 acatggggga tcatgtaact cgccttgatc gttgggaacc ggagctgaat gaagccatac
     8101 caaacgacga gcgtgacacc acgatgcctg tagcaatggc aacaacgttg cgcaaactat
     8161 taactggcga actacttact ctagcttccc ggcaacaatt aatagactgg atggaggcgg
     8221 ataaagttgc aggaccactt ctgcgctcgg cccttccggc tggctggttt attgctgata
     8281 aatctggagc cggtgagcgt gggtctcgcg gtatcattgc agcactgggg ccagatggta
     8341 agccctcccg tatcgtagtt atctacacga cggggagtca ggcaactatg gatgaacgaa
     8401 atagacagat cgctgagata ggtgcctcac tgattaagca ttggtaactg tcagaccaag
     8461 tttactcata tatactttag attgatttac cccggttgat aatcagaaaa gccccaaaaa
     8521 caggaagatt gtataagcaa atatttaaat tgtaaacgtt aatattttgt taaaattcgc
     8581 gttaaatttt tgttaaatca gctcattttt taaccaatag gccgaaatcg gcaaaatccc
     8641 ttataaatca aaagaatagc ccgagatagg gttgagtgtt gttccagttt ggaacaagag
     8701 tccactatta aagaacgtgg actccaacgt caaagggcga aaaaccgtct atcagggcga
     8761 tggcccacta cgtgaaccat cacccaaatc aagttttttg gggtcgaggt gccgtaaagc
     8821 actaaatcgg aaccctaaag ggagcccccg atttagagct tgacggggaa agccggcgaa
     8881 cgtggcgaga aaggaaggga agaaagcgaa aggagcgggc gctagggcgc tggcaagtgt
     8941 agcggtcacg ctgcgcgtaa ccaccacacc cgccgcgctt aatgcgccgc tacagggcgc
     9001 gtaaaaggat ctaggtgaag atcctttttg ataatctcat gaccaaaatc ccttaacgtg
     9061 agttttcgtt ccactgagcg tcagaccccg tagaaaagat caaaggatct tc
//
