LOCUS       sequence-458343-        3506 bp    DNA     circular SYN 15-APR-2025
DEFINITION  pBAD promoter controls the expression of sfGFP. In the presence of 
            VVD-AraC, blue light and L-arabinose can be used to activate 
            mCitrine expression..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3506)
  AUTHORS   Hollo G, Park JH, Boni E, Schaerli Y
  TITLE     GRN_modeler: An Intuitive Tool for Constructing and Evaluating Gene 
            Regulatory Networks and its Applications to Oscillators and a Light 
            Biosensor
  JOURNAL   bioRxiv 2024.12.18.629005
REFERENCE   2  (bases 1 to 3506)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "bioRxiv 
            2024.12.18.629005"
COMMENT     Sequence Label: pJP_1node
FEATURES             Location/Qualifiers
     source          1..3506
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    183..202
                     /label=Csy4 Site
                     /bound_moiety="Pseudomonas aeruginosa Csy4 (Cas6f)"
                     /note="cleavage site for the Csy4 endoribonuclease (Cermak 
                     et al. 2017)"
     RBS             207..229
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             237..950
                     /codon_start=1
                     /product="yeast-enhanced green fluorescent protein"
                     /label=yeGFP
                     /note="codons optimized for translation in S. cerevisiae 
                     and C. albicans"
                     /translation="MSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTLGYGLMCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSYQSKLSKDPNEKRDHMVLLE
                     FVTAAGITHGMDELYK"
     CDS             complement(1497..2312)
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     CDS             complement(1497..2312)
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     2220..2239
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     promoter        complement(2313..2404)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(2422..3057)
                     /direction=LEFT
                     /label=ColA ori
                     /note="Plasmids containing the ColA origin of replication 
                     can be propagated in E. coli cells that contain additional 
                     plasmids with compatible origins."
     primer_bind     complement(2567..2586)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     promoter        3212..3496
                     /gene="araBAD"
                     /label=araBAD promoter
                     /note="promoter of the L-arabinose operon of E. coli; the 
                     araC regulatory gene is transcribed in the opposite 
                     direction (Guzman et al., 1995)"
     primer_bind     3419..3438
                     /label=pBAD Forward
                     /note="For vectors with E. coli araBAD promoter, forward 
                     primer"
ORIGIN
        1 catttcaccc aaggagctcg agatagccgt tacacaggtg acacttattt cagcctgcgg
       61 tccggccaca ctagagtcta gcttgagatc gctgggacgc ccgtgaacag ttgctctgat
      121 tgaaaccacg attcagcaag agatttctac acgattgagc actgtctcag cctgcggtcc
      181 gggttcactg ccgtataggc agtaattttg tttaacttta agaaggagat atacatatgt
      241 ctaaaggtga agaattattc actggtgttg tcccaatttt ggttgaatta gatggtgatg
      301 ttaatggtca caaattttct gtctccggtg aaggtgaagg tgatgctact tacggtaaat
      361 tgaccttaaa atttatttgt actactggta aattgccagt tccgtggcca accttagtca
      421 ctactttagg ttatggtttg atgtgttttg ctagataccc agatcatatg aaacaacatg
      481 actttttcaa gtctgccatg ccagaaggtt atgttcaaga aagaactatt tttttcaaag
      541 atgacggtaa ctacaagacc agagctgaag tcaagtttga aggtgatacc ttagttaata
      601 gaatcgaatt aaaaggtatt gattttaaag aagatggtaa cattttaggt cacaaattgg
      661 aatacaacta taactctcac aatgtttaca tcatggctga caaacaaaag aatggtatca
      721 aagttaactt caaaattaga cacaacattg aagatggttc tgttcaatta gctgaccatt
      781 atcaacaaaa tactccaatt ggtgatggtc cagtcttgtt accagacaac cattacttat
      841 cctatcaatc taaattatcc aaagatccaa acgaaaagag agaccacatg gtcttgttag
      901 aatttgttac tgctgctggt attacccacg gtatggatga attgtacaaa atgtcccgcc
      961 gtaatactga cgccatcaca atccacagca tcctggattg gattgaagac taataatcgc
     1021 tgggacgccc gcctgcaggc tcggtaccaa attccagaaa agaggcctcc cgaaaggggg
     1081 gccttttttc gttttggtcc taatagataa aggataggtc tggtagtgtt gttcgttctc
     1141 gcaggtaaat caataatact cagcagttcc gtagactttt cagtgggaca gggtagcgat
     1201 aacagataga ttgtaataag acacagtagg tgctcgtagt tgcgtgaaga gaaccgctca
     1261 ggaaatccag tcagaagtat tggtaatcgt tgtttctgcg caatcgttgt gcgtttcaga
     1321 tctaccggta aaccagcaat agacataagc ggctatttaa cgaccctgcc ctgaaccgac
     1381 gacaagctga cgaccgggtc tccgcaagtg gcacttttcg gggaaatgtg cgcggaaccc
     1441 ctatttgttt atttttctaa atacattcaa atatgtatcc gctcatgaat taattcttag
     1501 aaaaactcat cgagcatcaa atgaaactgc aatttattca tatcaggatt atcaatacca
     1561 tatttttgaa aaagccgttt ctgtaatgaa ggagaaaact caccgaggca gttccatagg
     1621 atggcaagat cctggtatcg gtctgcgatt ccgactcgtc caacatcaat acaacctatt
     1681 aatttcccct cgtcaaaaat aaggttatca agtgagaaat caccatgagt gacgactgaa
     1741 tccggtgaga atggcaaaag tttatgcatt tctttccaga cttgttcaac aggccagcca
     1801 ttacgctcgt catcaaaatc actcgcatca accaaaccgt tattcattcg tgattgcgcc
     1861 tgagcgagac gaaatacgcg gtcgctgtta aaaggacaat tacaaacagg aatcgaatgc
     1921 aaccggcgca ggaacactgc cagcgcatca acaatatttt cacctgaatc aggatattct
     1981 tctaatacct ggaatgctgt tttcccgggg atcgcagtgg tgagtaacca tgcatcatca
     2041 ggagtacgga taaaatgctt gatggtcgga agaggcataa attccgtcag ccagtttagt
     2101 ctgaccatct catctgtaac atcattggca acgctacctt tgccatgttt cagaaacaac
     2161 tctggcgcat cgggcttccc atacaatcga tagattgtcg cacctgattg cccgacatta
     2221 tcgcgagccc atttataccc atataaatca gcatccatgt tggaatttaa tcgcggccta
     2281 gagcaagacg tttcccgttg aatatggctc atactcttcc tttttcaata ttattgaagc
     2341 atttatcagg gttattgtct catgagcgga tacatatttg aatgtattta gaaaaataaa
     2401 caaataggca tgctagcgca gaaacgtcct agaagatgcc aggaggatac ttagcagaga
     2461 gacaataagg ccggagcgaa gccgtttttc cataggctcc gcccccctga cgaacatcac
     2521 gaaatctgac gctcaaatca gtggtggcga aacccgacag gactataaag ataccaggcg
     2581 tttccccctg atggctccct cttgcgctct cctgttcccg tcctgcggcg tccgtgttgt
     2641 ggtggaggct ttacccaaat caccacgtcc cgttccgtgt agacagttcg ctccaagctg
     2701 ggctgtgtgc aagaaccccc cgttcagccc gactgctgcg ccttatccgg taactatcat
     2761 cttgagtcca acccggaaag acacgacaaa acgccactgg cagcagccat tggtaactga
     2821 gaattagtgg atttagatat cgagagtctt gaagtggtgg cctaacagag gctacactga
     2881 aaggacagta tttggtatct gcgctccact aaagccagtt accaggttaa gcagttcccc
     2941 aactgactta accttcgatc aaaccgcctc cccaggcggt tttttcgttt acagagcagg
     3001 agattacgac gatcgtaaaa ggatctcaag aagatccttt acggattccc gacaccatca
     3061 ctctagattt cagtgcaatt tatctcttca aatgtagcac ctgaagtcag ccccatacga
     3121 tataagttgt aattctcatg ttagtcatgc cccgcgccca ccggaaggag ctgactgggt
     3181 tgctcacttt tcatactccc gccattcaga gaagaaacca attgtccata ttgcatcaga
     3241 cattgccgtc actgcgtctt ttactggctc ttctcgctaa ccaaaccggt aaccccgctt
     3301 attaaaagca ttctgtaaca aagcgggacc aaagccatga caaaaacgcg taacaaaagt
     3361 gtctataatc acggcagaaa agtccacatt gattatttgc acggcgtcac actttgctat
     3421 gccatagcat ttttatccat aagattagcg gttcctacct gacgcttttt atcgcaactc
     3481 tctactgttt ctccataccc tgaatt
//
