LOCUS       sequence-458495-        8969 bp    DNA     circular SYN 14-APR-2025
DEFINITION  Encodes a 4xmNeonGreen-dMCP under control of a weak promoter (UBC)..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8969)
  AUTHORS   Kuffner CJ, Marzilli AM, Ngo JT
  TITLE     RNA-Stabilized Coat Proteins for Sensitive and Simultaneous Imaging 
            of Distinct Single mRNAs in Live Cells
  JOURNAL   bioRxiv 2024.11.21.624393
REFERENCE   2  (bases 1 to 8969)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 14, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "bioRxiv 
            2024.11.21.624393"
COMMENT     Sequence Label: UBC-4xmNG-dMCP
FEATURES             Location/Qualifiers
     source          1..8969
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        31..429
                     /label=UbC promoter
                     /note="human ubiquitin C promoter"
     regulatory      436..445
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             442..1146
                     /codon_start=1
                     /product="bright monomeric yellow-green fluorescent protein
                     derived from LanYFP (Shaner et al., 2013)"
                     /label=mNeonGreen
                     /translation="MVSKGEEDNMASLPATHELHIFGSINGVDFDMVGQGTGNPNDGYE
                     ELNLKSTKGDLQFSPWILVPHIGYGFHQYLPYPDGMSPFQAAMVDGSGYQVHRTMQFED
                     GASLTVNYRYTYEGSHIKGEAQVKGTGFPADGPVMTNSLTAADWCRSKKTYPNDKTIIS
                     TFKWSYTTGNGKRYRSTARTTYTFAKPMAANYLKNQPMYVFRKTELKHSKTELNFKEWQ
                     KAFTDVMGMDELY"
     regulatory      697..706
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             2692..3396
                     /codon_start=1
                     /product="bright monomeric yellow-green fluorescent protein
                     derived from LanYFP (Shaner et al., 2013)"
                     /label=mNeonGreen
                     /translation="VSKGEEDNMASLPATHELHIFGSINGVDFDMVGQGTGNPNDGYEE
                     LNLKSTKGDLQFSPWILVPHIGYGFHQYLPYPDGMSPFQAAMVDGSGYQVHRTMQFEDG
                     ASLTVNYRYTYEGSHIKGEAQVKGTGFPADGPVMTNSLTAADWCRSKKTYPNDKTIIST
                     FKWSYTTGNGKRYRSTARTTYTFAKPMAANYLKNQPMYVFRKTELKHSKTELNFKEWQK
                     AFTDVMGMDELYK"
     regulatory      2944..2953
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             3406..3432
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /note="This is a variant of the standard HA tag, which has 
                     sequence YPYDVPDYA. Some anti-HA antibodies may not 
                     recognize this variant."
                     /translation="YPYDVPDYA"
     CDS             3406..3432
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     primer_bind     complement(4187..4204)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    4193..4417
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      4463..4891
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4550..4569)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     4760..4781
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(4900..4920)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        4905..5234
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      5085..5220
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     5147..5166
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     CDS             5283..6308
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     CDS             5283..6308
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     polyA_signal    6438..6559
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6475..6494)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     6529..6548
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(6608..6624)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6608..6624)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(6621..6643)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    6632..6648
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(6656..6662,6663..6680,6681..6686))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6701..6722
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(6839..6856)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7010..7598)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7090..7109)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(7769..8560,8561..8629))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8392..8411
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8630..8734)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(8809..8828)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
ORIGIN
        1 ttcgcgatgt acgggccaga tatacgcgtt ggcctccgcg ccgggttttg gcgcctcccg
       61 cgggcgcccc cctcctcacg gcgagcgctg ccacgtcaga cgaagggcgc agcgagcgtc
      121 ctgatccttc cgcccggacg ctcaggacag cggcccgctg ctcataagac tcggccttag
      181 aaccccagta tcagcagaag gacattttag gacgggactt gggtgactct agggcactgg
      241 ttttctttcc agagagcgga acaggcgagg aaaagtagtc ccttctcggc gattctgcgg
      301 agggatctcc gtggggcggt gaacgccgat gattatataa ggacgcgccg ggtgtggcac
      361 agctagttcc gtcgcagccg ggatttgggt cgcggttctt gtttgtggat cgctgtgatc
      421 gtcacttgga agcttgccac catggtgagc aagggcgagg aggataacat ggcctctctc
      481 ccagcgacac atgagttaca catctttggc tccatcaacg gtgtggactt tgacatggtg
      541 ggtcagggca ccggcaatcc aaatgatggt tatgaggagt taaacctgaa gtccaccaag
      601 ggtgacctcc agttctcccc ctggattctg gtccctcata tcgggtatgg cttccatcag
      661 tacctgccct accctgacgg gatgtcgcct ttccaggccg ccatggtaga tggctccgga
      721 taccaagtcc atcgcacaat gcagtttgaa gatggtgcct cccttactgt taactaccgc
      781 tacacctacg agggaagcca catcaaagga gaggcccagg tgaaggggac tggtttccct
      841 gctgacggtc ctgtgatgac caactcgctg accgctgcgg actggtgcag gtcgaagaag
      901 acttacccca acgacaaaac catcatcagt acctttaagt ggagttacac cactggaaat
      961 ggcaagcgct accggagcac tgcgcggacc acctacacct ttgccaagcc aatggcggct
     1021 aactatctga agaaccagcc gatgtacgtg ttccgtaaga cggagctcaa gcactccaag
     1081 accgagctca acttcaagga gtggcaaaag gcctttaccg atgtgatggg catggacgag
     1141 ctgtacgagg gtggacatat gggcacaggg tccacaggcg gtaccggcgg agtttccaaa
     1201 ggagaagaag acaatatggc atcactcccc gcaacccacg agttgcatat tttcggttca
     1261 attaatggag tagatttcga tatggttggc cagggaacag gaaacccaaa cgacggatat
     1321 gaagagctta atctcaaaag taccaaaggc gatctgcaat tttctccgtg gatactcgtg
     1381 ccacacattg gatacggatt tcaccaatat ctcccgtatc cggatggaat gtcccccttt
     1441 caagcagcaa tggtggacgg gagtggttat caggtacaca gaaccatgca gttcgaggac
     1501 ggggcttctc tgaccgtaaa ttataggtat acttatgaag gctcacatat taagggcgaa
     1561 gcacaggtta aaggaaccgg gtttcctgcg gatggccccg tcatgactaa ttctctgaca
     1621 gccgcagatt ggtgtcgctc caaaaagaca tacccgaatg ataagactat aatctcaaca
     1681 ttcaaatggt cctatacgac aggcaacggg aaacgatata gatccacggc tcgaacaact
     1741 tacacattcg ctaaacctat ggccgccaat tacctcaaaa atcagcccat gtatgtgttt
     1801 aggaaaaccg aattgaagca ttctaaaacg gaacttaatt ttaaggaatg gcagaaggct
     1861 ttcacagacg taatggggat ggatgaactc tataaatcag gtctcgagtc ctcaggggga
     1921 acgggtgggt ccggaggagt tagtaaaggt gaagaggaca atatggcaag tttgcctgcg
     1981 actcacgagc ttcatatctt tgggtctata aatggcgttg acttcgatat ggttggccaa
     2041 ggtactggca accccaatga cggttacgag gagttgaatc tcaagtccac aaaaggtgat
     2101 cttcagttca gcccttggat tctcgtacct catattggat atggctttca ccagtacctt
     2161 ccatacccag acggtatgtc accctttcaa gctgcgatgg tggatggttc cggctatcag
     2221 gtccaccgaa cgatgcaatt cgaggacggg gccagcctca ccgttaatta taggtacacc
     2281 tatgagggaa gtcacataaa gggagaagcc caagtgaaag gaacaggatt cccagctgat
     2341 ggtccagtaa tgacgaactc cttgacagcg gctgactggt gtagaagcaa aaagacgtat
     2401 cctaatgaca agaccatcat tagcactttc aaatggagtt ataccacagg aaacggcaaa
     2461 cggtacagaa gcactgctag aactacctac actttcgcaa agccgatggc tgcaaactat
     2521 ttgaagaatc agcccatgta cgtgtttcga aaaacggaac ttaagcacag taagactgaa
     2581 cttaatttca aggagtggca gaaggcgttc acggatgtca tgggtatgga tgaactgtat
     2641 aagggagggt ctggcactgg gggcactgcc agcagcggat ccggtggcgg tgtgagcaag
     2701 ggcgaggagg ataacatggc ctctctccca gcgacacatg agttacacat ctttggctcc
     2761 atcaacggtg tggactttga catggtgggt cagggcaccg gcaatccaaa tgatggttat
     2821 gaggagttaa acctgaagtc caccaagggt gacctccagt tctccccctg gattctggtc
     2881 cctcatatcg ggtatggctt ccatcagtac ctgccctacc ctgacgggat gtcgcctttc
     2941 caggccgcca tggtagatgg ctccggatac caagtccatc gcacaatgca gtttgaagat
     3001 ggtgcctccc ttactgttaa ctaccgctac acctacgagg gaagccacat caaaggagag
     3061 gcccaggtga aggggactgg tttccctgct gacggtcctg tgatgaccaa ctcgctgacc
     3121 gctgcggact ggtgcaggtc gaagaagact taccccaacg acaaaaccat catcagtacc
     3181 tttaagtgga gttacaccac tggaaatggc aagcgctacc ggagcactgc gcggaccacc
     3241 tacacctttg ccaagccaat ggcggctaac tatctgaaga accagccgat gtacgtgttc
     3301 cgtaagacgg agctcaagca ctccaagacc gagctcaact tcaaggagtg gcaaaaggcc
     3361 tttaccgatg tgatgggcat ggacgagctg tacaagggcg ggagttaccc ctacgacgtg
     3421 cccgactacg ccggtaccgg cgggagtggc ggtagtgccc aaaacaggaa atacaccatc
     3481 aaagtggagg tgccaaaggg tgcctggcgg agctacctga acatggagct gactattcca
     3541 atcttcgcta ctaattccga ttgcgaactg atagtaaaag cgatgcaagg tttgctgaaa
     3601 gacggcaacc caattcccag cgcaatcgcc gccaatagcg gcatctacgc taatttcaca
     3661 cagttcgtgc tggtcgacaa cggcggcacc ggcgacgtta cggtggcccc ctcaaacttt
     3721 gccaacggga ttgccgagtg gataagcagc aatagcaggt cccaggccta caaggttacc
     3781 tgtagcgtaa ggcagagcag cgcccagaac cgaaagtaca cgattaaggt ggaagtcccc
     3841 aaaggcgcat ggaggagcta tcttaatatg gaactgacca tccccatatt cgcgacaaac
     3901 agcgactgtg agctgatcgt gaaggctatg cagggcctcc tcaaggatgg gaacccgatc
     3961 ccgtctgcca tcgctgctaa ctccggcatt tatgcaaact tcactcaatt tgttctggtg
     4021 gacaatggtg ggaccgggga tgttaccgtg gctcccagca attttgctaa cggtatcgca
     4081 gaatggatca gtagtaacag caggagccaa gcctataaag tgacgtgcag cgtacggcag
     4141 aggagaagag gctaatctag agggcccgtt taaacccgct gatcagcctc gactgtgcct
     4201 tctagttgcc agccatctgt tgtttgcccc tcccccgtgc cttccttgac cctggaaggt
     4261 gccactccca ctgtcctttc ctaataaaat gaggaaattg catcgcattg tctgagtagg
     4321 tgtcattcta ttctgggggg tggggtgggg caggacagca agggggagga ttgggaagac
     4381 aatagcaggc atgctgggga tgcggtgggc tctatggctt ctgaggcgga aagaaccagc
     4441 tggggctcta gggggtatcc ccacgcgccc tgtagcggcg cattaagcgc ggcgggtgtg
     4501 gtggttacgc gcagcgtgac cgctacactt gccagcgccc tagcgcccgc tcctttcgct
     4561 ttcttccctt cctttctcgc cacgttcgcc ggctttcccc gtcaagctct aaatcggggg
     4621 ctccctttag ggttccgatt tagtgcttta cggcacctcg accccaaaaa acttgattag
     4681 ggtgatggtt cacgtagtgg gccatcgccc tgatagacgg tttttcgccc tttgacgttg
     4741 gagtccacgt tctttaatag tggactcttg ttccaaactg gaacaacact caaccctatc
     4801 tcggtctatt cttttgattt ataagggatt ttgccgattt cggcctattg gttaaaaaat
     4861 gagctgattt aacaaaaatt taacgcgaat taattctgtg gaatgtgtgt cagttagggt
     4921 gtggaaagtc cccaggctcc ccagcaggca gaagtatgca aagcatgcat ctcaattagt
     4981 cagcaaccag gtgtggaaag tccccaggct ccccagcagg cagaagtatg caaagcatgc
     5041 atctcaatta gtcagcaacc atagtcccgc ccctaactcc gcccatcccg cccctaactc
     5101 cgcccagttc cgcccattct ccgccccatg gctgactaat tttttttatt tatgcagagg
     5161 ccgaggccgc ctctgcctct gagctattcc agaagtagtg aggaggcttt tttggaggcc
     5221 taggcttttg caaaaagctc ccgggagctt gtatatccat tttcggatct gatcagcacg
     5281 tgatgaaaaa gcctgaactc accgcgacgt ctgtcgagaa gtttctgatc gaaaagttcg
     5341 acagcgtctc cgacctgatg cagctctcgg agggcgaaga atctcgtgct ttcagcttcg
     5401 atgtaggagg gcgtggatat gtcctgcggg taaatagctg cgccgatggt ttctacaaag
     5461 atcgttatgt ttatcggcac tttgcatcgg ccgcgctccc gattccggaa gtgcttgaca
     5521 ttggggaatt cagcgagagc ctgacctatt gcatctcccg ccgtgcacag ggtgtcacgt
     5581 tgcaagacct gcctgaaacc gaactgcccg ctgttctgca gccggtcgcg gaggccatgg
     5641 atgcgatcgc tgcggccgat cttagccaga cgagcgggtt cggcccattc ggaccgcaag
     5701 gaatcggtca atacactaca tggcgtgatt tcatatgcgc gattgctgat ccccatgtgt
     5761 atcactggca aactgtgatg gacgacaccg tcagtgcgtc cgtcgcgcag gctctcgatg
     5821 agctgatgct ttgggccgag gactgccccg aagtccggca cctcgtgcac gcggatttcg
     5881 gctccaacaa tgtcctgacg gacaatggcc gcataacagc ggtcattgac tggagcgagg
     5941 cgatgttcgg ggattcccaa tacgaggtcg ccaacatctt cttctggagg ccgtggttgg
     6001 cttgtatgga gcagcagacg cgctacttcg agcggaggca tccggagctt gcaggatcgc
     6061 cgcggctccg ggcgtatatg ctccgcattg gtcttgacca actctatcag agcttggttg
     6121 acggcaattt cgatgatgca gcttgggcgc agggtcgatg cgacgcaatc gtccgatccg
     6181 gagccgggac tgtcgggcgt acacaaatcg cccgcagaag cgcggccgtc tggaccgatg
     6241 gctgtgtaga agtactcgcc gatagtggaa accgacgccc cagcactcgt ccgagggcaa
     6301 aggaatagca cgtgctacga gatttcgatt ccaccgccgc cttctatgaa aggttgggct
     6361 tcggaatcgt tttccgggac gccggctgga tgatcctcca gcgcggggat ctcatgctgg
     6421 agttcttcgc ccaccccaac ttgtttattg cagcttataa tggttacaaa taaagcaata
     6481 gcatcacaaa tttcacaaat aaagcatttt tttcactgca ttctagttgt ggtttgtcca
     6541 aactcatcaa tgtatcttat catgtctgta taccgtcgac ctctagctag agcttggcgt
     6601 aatcatggtc atagctgttt cctgtgtgaa attgttatcc gctcacaatt ccacacaaca
     6661 tacgagccgg aagcataaag tgtaaagcct ggggtgccta atgagtgagc taactcacat
     6721 taattgcgtt gcgctcactg cccgctttcc agtcgggaaa cctgtcgtgc cagctgcatt
     6781 aatgaatcgg ccaacgcgcg gggagaggcg gtttgcgtat tgggcgctct tccgcttcct
     6841 cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg agcggtatca gctcactcaa
     6901 aggcggtaat acggttatcc acagaatcag gggataacgc aggaaagaac atgtgagcaa
     6961 aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc
     7021 tccgcccccc tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga
     7081 caggactata aagataccag gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc
     7141 cgaccctgcc gcttaccgga tacctgtccg cctttctccc ttcgggaagc gtggcgcttt
     7201 ctcatagctc acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc aagctgggct
     7261 gtgtgcacga accccccgtt cagcccgacc gctgcgcctt atccggtaac tatcgtcttg
     7321 agtccaaccc ggtaagacac gacttatcgc cactggcagc agccactggt aacaggatta
     7381 gcagagcgag gtatgtaggc ggtgctacag agttcttgaa gtggtggcct aactacggct
     7441 acactagaag aacagtattt ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa
     7501 gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt
     7561 gcaagcagca gattacgcgc agaaaaaaag gatctcaaga agatcctttg atcttttcta
     7621 cggggtctga cgctcagtgg aacgaaaact cacgttaagg gattttggtc atgagattat
     7681 caaaaaggat cttcacctag atccttttaa attaaaaatg aagttttaaa tcaatctaaa
     7741 gtatatatga gtaaacttgg tctgacagtt accaatgctt aatcagtgag gcacctatct
     7801 cagcgatctg tctatttcgt tcatccatag ttgcctgact ccccgtcgtg tagataacta
     7861 cgatacggga gggcttacca tctggcccca gtgctgcaat gataccgcga gacccacgct
     7921 caccggctcc agatttatca gcaataaacc agccagccgg aagggccgag cgcagaagtg
     7981 gtcctgcaac tttatccgcc tccatccagt ctattaattg ttgccgggaa gctagagtaa
     8041 gtagttcgcc agttaatagt ttgcgcaacg ttgttgccat tgctacaggc atcgtggtgt
     8101 cacgctcgtc gtttggtatg gcttcattca gctccggttc ccaacgatca aggcgagtta
     8161 catgatcccc catgttgtgc aaaaaagcgg ttagctcctt cggtcctccg atcgttgtca
     8221 gaagtaagtt ggccgcagtg ttatcactca tggttatggc agcactgcat aattctctta
     8281 ctgtcatgcc atccgtaaga tgcttttctg tgactggtga gtactcaacc aagtcattct
     8341 gagaatagtg tatgcggcga ccgagttgct cttgcccggc gtcaatacgg gataataccg
     8401 cgccacatag cagaacttta aaagtgctca tcattggaaa acgttcttcg gggcgaaaac
     8461 tctcaaggat cttaccgctg ttgagatcca gttcgatgta acccactcgt gcacccaact
     8521 gatcttcagc atcttttact ttcaccagcg tttctgggtg agcaaaaaca ggaaggcaaa
     8581 atgccgcaaa aaagggaata agggcgacac ggaaatgttg aatactcata ctcttccttt
     8641 ttcaatatta ttgaagcatt tatcagggtt attgtctcat gagcggatac atatttgaat
     8701 gtatttagaa aaataaacaa ataggggttc cgcgcacatt tccccgaaaa gtgccacctg
     8761 acgtcgacgg atcgggagat ctcccgatcc cctatggtgc actctcagta caatctgctc
     8821 tgatgccgca tagttaagcc agtatctgct ccctgcttgt gtgttggagg tcgctgagta
     8881 gtgcgcgagc aaaatttaag ctacaacaag gcaaggcttg accgacaatt gcatgaagaa
     8941 tctgcttagg gttaggcgtt ttgcgctgc
//
