LOCUS       sequence-458764-         864 bp    DNA     linear   UNA 15-APR-2025
DEFINITION  Retroviral expression of tagged mNgn2x3FLAG.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 864)
  AUTHORS   Wang NB, Adewumi HO, Lende-Dorn BA, Beitz AM, O'Shea TM, Galloway KE
  TITLE     Compact transcription factor cassettes generate functional, 
            engraftable motor neurons by direct conversion.
  JOURNAL   Cell Syst. 2025 Mar 10:101206. doi: 10.1016/j.cels.2025.101206.
  PUBMED    40086435
REFERENCE   2  (bases 1 to 864)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell Syst. 
            2025 Mar 10:101206. doi: 10.1016/j.cels.2025.101206."
COMMENT     Sequence Label: pMXs-mNgn2-x3FLAG
FEATURES             Location/Qualifiers
     source          1..864
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             799..864
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
ORIGIN
        1 atgttcgtca aatctgagac tctggagttg aaggaggaag aggaggtact gatgctgctg
       61 ggctcggctt ccccggcctc ggcgaccctg accccgatgt cctccagcgc ggacgaggag
      121 gaggacgagg agctgcgccg gccgggctcc gcgcgtgggc agcgtggagc ggaagccggg
      181 cagggggtgc agggcagtcc ggcgtcgggt gccgggggtt gccggccagg gcggctgctg
      241 ggcctgatgc acgagtgcaa gcgtcgcccg tcgcgctcac gggccgtctc ccgaggtgcc
      301 aagacggcgg agacggtgca gcgcatcaag aagacccgca ggctcaaggc caacaaccgc
      361 gagcgcaacc gcatgcacaa cctaaacgcc gcgctggacg cgctgcgcga ggtgctgccc
      421 accttccccg aggatgccaa gctcacgaag atcgagacgc tgcgcttcgc ccacaattac
      481 atctgggcgc tcaccgagac tctgcgcctg gcggaccact gcgccggcgc cggtggcctc
      541 cagggggcgc tcttcacgga ggcggtgctc ctgagcccgg gagctgcgct cggcgccagc
      601 ggggacagcc cttctccacc ttcctcctgg agctgcacca acagcccggc gtcatcctcc
      661 aactccacgt ccccatacag ctgcacttta tcgcccgcta gccccgggtc agacgtggac
      721 tactggcagc ccccacctcc ggagaagcat cgttatgcgc ctcacctgcc cctcgccagg
      781 gactgtatcg gaagcggtga ttataaggat cacgatggcg actacaagga ccacgatatt
      841 gactacaaag acgatgacga taaa
//
