LOCUS       sequence-459840-       14335 bp    DNA     circular SYN 15-APR-2025
DEFINITION  Introduce a C-to-T mutation to generate an early stop codon (Q139 to
            stop), thereby interrupting RUBY expression.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 14335)
  TITLE     Seeing is believing: Genome editing made easy with RUBY and GFP for 
            educational purposes
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 14335)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: RUBYm2
FEATURES             Location/Qualifiers
     source          1..14335
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        32..708
                     /label=CaMV 35S promoter (enhanced)
                     /note="cauliflower mosaic virus 35S promoter with a 
                     duplicated enhancer region"
     primer_bind     645..665
                     /label=35S promoter
                     /note="CaMV 35S promoter, forward primer"
     CDS             717..4667
                     /label=RUBY
     CDS             2217..2273
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             3108..3164
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     promoter        5371..5550
                     /label=NOS promoter
                     /note="nopaline synthase promoter"
     promoter        5403..5586
                     /function="NOS promoter"
                     /label=NOS promoter
                     /note="nopaline synthase promoter"
     terminator      6701..6953
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     primer_bind     complement(7528..7545)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(7528..7544)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7537..7559)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     misc_feature    7766..7790
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     CDS             8311..9102
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MGEAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      9348..9936
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     9837..9856
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     10090..10107
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    10122..10262
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(10188..10207)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     10348..10370
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     rep_origin      10606..10800
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     CDS             complement(10866..11939)
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     CDS             complement(12368..12997)
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
     misc_feature    join(14328..14335,1..17)
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
ORIGIN
        1 gccaatatat cctgtcaaac actgatagtt ttgagacttt tcaacaaagg gtaatatcgg
       61 gaaacctcct cggattccat tgcccagcta tctgtcactt catcaaaagg acagtagaaa
      121 aggaaggtgg cacctacaaa tgccatcatt gcgataaagg aaaggctatc gttcaagatg
      181 cctctgccga cagtggtccc aaagatggac ccccacccac gaggagcatc gtggaaaaag
      241 aagacgttcc aaccacgtct tcaaagcaag tggattgatg tgaacatggt ggagcacgac
      301 actctcgtct actccaagaa tatcaaagat acagtctcag aagaccaaag ggctattgag
      361 acttttcaac aaagggtaat atcgggaaac ctcctcggat tccattgccc agctatctgt
      421 cacttcatca aaaggacagt agaaaaggaa ggtggcacct acaaatgcca tcattgcgat
      481 aaaggaaagg ctatcgttca agatgcctct gccgacagtg gtcccaaaga tggaccccca
      541 cccacgagga gcatcgtgga aaaagaagac gttccaacca cgtcttcaaa gcaagtggat
      601 tgatgtgata tctccactga cgtaagggat gacgcacaat cccactatcc ttcgcaagac
      661 ccttcctcta tataaggaag ttcatttcat ttggagagga cacgctgaaa actagtatgg
      721 atcatgcgac cctcgccatg atcctcgcga tctggttcat cagcttccac ttcatcaagc
      781 tgctgttctc ccagcagacc accaagctgc ttccgccagg accaaagccg cttccgatca
      841 tcggcaacat ccttgaggtg ggcaagaagc cgcatcggtc cttcgccaac ctcgccaaga
      901 ttcacggccc actcatttcc ctcagactcg gctctgtgac caccatcgtt gtgtcctctg
      961 ccgacgtggc caaagagatg ttcctcaaga aggatcaccc gctctccaac cgcacgatcc
     1021 cgaatagtgt tacagccggc gaccaccaca agctcaccat gtcttggctc ccggtgtctc
     1081 cgaagtggcg caacttccgc aagattaccg ccgtgcatct gctctcccca tagagactcg
     1141 atgcctgcca gacattcagg cacgccaagg tgcagcagct ctacgagtac gttcaagagt
     1201 gcgcccagaa aggccaggcc gtggatattg gcaaggccgc ctttacgacc agcctcaacc
     1261 tcctcagcaa gctgttcttc agcgtcgagc tggcgcacca caagtcccat accagccaag
     1321 agttcaaaga gctgatctgg aacatcatgg aagatatagg caagccgaac tacgccgact
     1381 acttcccgat tctcggctgc gttgacccat ctggcattag aagaaggctc gcctgctcct
     1441 tcgacaagct gatcgccgtg ttccagggca tcatctgcga gagactcgcc ccagattcct
     1501 ccaccacaac taccaccacc accgacgacg tgctcgatgt gctcctccag ctgttcaagc
     1561 agaacgagct gacgatgggc gagatcaacc acctcctcgt ggacatcttc gacgccggca
     1621 ccgataccac atcctccaca ttcgagtggg tgatgaccga gctgatccgc aatccagaga
     1681 tgatggaaaa ggcccaagag gaaatcaagc aggtcctcgg caaggacaag cagatccaag
     1741 agtccgacat catcaacctg ccgtacctcc aggcgatcat caaagagaca ctccgcctcc
     1801 atccgccgac cgtgttcttg ctcccaagaa aggccgacac cgatgtcgag ctgtacggct
     1861 acatcgtgcc gaaggatgcc cagatcctcg tgaacctctg ggccattggc agggacccaa
     1921 acgcctggca gaacgccgat attttcagcc cagagcgctt catcggctgc gagatcgatg
     1981 ttaagggccg cgatttcggc ctccttccat ttggcgctgg ccgcagaatt tgcccaggca
     2041 tgaatctcgc catcaggatg ctcaccctca tgctcgccac actcctccag ttcttcaact
     2101 ggaagctcga aggcgacatc tccccgaagg acctcgacat ggacgagaag ttcggcattg
     2161 cgctccaaaa gaccaagccg ctcaagctca tcccgattcc gcgctacggt agcggagcta
     2221 ccaattttag cctccttaag caggcaggtg atgtagaaga gaaccccggg cctatgaaga
     2281 tgatgaacgg cgaggacgcc aacgaccaga tgatcaaaga gtccttcttc atcacccacg
     2341 gcaacccgat cctcaccgtc gaggatacac atccgctcag gccgttcttc gagacatggc
     2401 gcgagaagat tttctccaag aagccgaagg ccatcctcat catctccggc cactgggaga
     2461 cagtgaagcc aaccgtgaac gccgtgcaca tcaacgacac catccacgac ttcgacgact
     2521 acccagccgc catgtaccag ttcaagtacc cagctccagg cgagccagag cttgcgagaa
     2581 aggtggaaga gatcctcaag aagtccgggt tcgagacagc cgagacagac caaaagaggg
     2641 gccttgatca cggcgcctgg gttccactca tgctcatgta tccagaggcg gacatcccgg
     2701 tgtgccagct ctcagttcag ccacatctcg acggcaccta ccactacaat ctcggcagag
     2761 ccctcgcgcc gctcaagaat gatggcgtgc tcattattgg ctccggcagc gccacacatc
     2821 cactcgatga gacaccgcac tacttcgatg gtgttgcccc ttgggccgct gccttcgatt
     2881 cttggcttag gaaggccctc atcaacggcc gcttcgagga agtgaacatc tacgagagca
     2941 aggccccgaa ctggaagctc gcccatccat ttccagagca cttctacccg ctccacgttg
     3001 tgctcggcgc tgctggtgaa aagtggaagg ccgagctgat ccactcctcc tgggatcatg
     3061 gcacactttg ccacggctcc tacaagttca cctccgccgg atccggagca accaacttta
     3121 gcctgctcaa gcaagcagga gatgttgagg aaaatcctgg ccccatgacc gccatcaaga
     3181 tgaacaccaa cggcgagggc gagacacagc acatcctcat gatcccgttc atggcgcagg
     3241 gccacctcag gccatttctc gaactcgcca tgttcctcta caagcgctcc cacgtgatca
     3301 tcaccctgct cacaactccg ctcaatgccg gcttcctcag gcacctcctt caccaccatt
     3361 cctactcctc cagcggcatc aggatcgtcg agctgccatt caactccacc aaccacggac
     3421 tcccaccggg catcgagaac accgataagc tcacactccc gctcgtggtg tccctcttcc
     3481 attccaccat cagcctcgat ccgcacctcc gcgattacat ctccaggcat ttcagcccag
     3541 ccaggccacc actctgcgtg atccatgatg tgttcctcgg ctgggttgac caggtggcca
     3601 aggatgtggg ctctacaggc gtggtgttca caacaggcgg cgcttatggc acatccgcct
     3661 acgtgtccat ctggaacgat ctcccgcacc agaactactc cgacgaccaa gagttcccgc
     3721 tgccaggctt cccagagaac cataagttcc gcaggtccca gctccatcgg ttcctcagat
     3781 atgccgacgg ctccgacgat tggtccaagt atttccagcc gcagctccgc cagtccatga
     3841 agtcttttgg ctggctctgc aactccgtgg aagagatcga gacactcggc ttctccatcc
     3901 tccgcaacta caccaagctg ccgatctggg gcatcggccc acttattgct tccccagtgc
     3961 agcactcctc ctccgacaac aattcaacag gcgccgagtt cgtgcagtgg ctcagcctca
     4021 aagagccgga ctccgtcctc tacatctcct tcggctccca gaacacgatc agcccgacgc
     4081 agatgatgga actcgctgct ggccttgagt cctccgagaa gccattcctc tgggtgatca
     4141 gagccccgtt cggcttcgac atcaacgaag agatgcgccc agagtggctg ccagagggct
     4201 ttgaggaacg catgaaggtg aagaaacagg gcaagctcgt gtacaagctc ggcccgcagc
     4261 ttgagatcct caaccatgaa tccatcggcg gctttctcac ccactgcgga tggaacagca
     4321 tccttgagtc tcttcgcgag ggcgttccga tgcttggatg gccacttgct gccgagcagg
     4381 cctacaacct caagtacctc gaagatgaga tgggcgtcgc ggttgagctt gctagaggcc
     4441 tcgaaggcga gatctccaaa gagaaggtca agcgcatcgt cgagatgatc cttgagcgca
     4501 acgagggctc caaaggctgg gagatgaaga atcgcgccgt ggaaatgggc aaaaagctca
     4561 aggacgccgt gaacgaggaa aaagagctga agggctcctc cgtgaaggcg atcgacgatt
     4621 tcctcgacgc cgtcatgcag gccaaacttg agccaagcct ccagtgatag tgaatatgaa
     4681 gatgaagatg aaatatttgg tgtgtcaaat aaaaaggttg tgtgcttaag tttgtgtttt
     4741 tttcttggct tgttgtgtta tgaatttgtg gctttttcta atattaaatg aatgtaacat
     4801 ctcattataa tgaataaaca aatgtttcta taatccattg tgaatgtttt gttggatctc
     4861 ttctccagca tataactact gtatgtgcta tggtatggac tatggaatat gattaaagat
     4921 aagacctaac tgagtaagct agcttgttcg agtattatgg cattgggaaa actgtttttc
     4981 ttgtaccatt tgttgtgctt gtaatttact gtgtttttta ttcggttttc gctatcgaac
     5041 tgtgaaatgg aaatggatgg agaagagtta atgaatgata tggtcctttt gttcattctc
     5101 aaattaatat tatttgtttt ttctcttatt tgttgtgtgt tgaatttgaa attataagag
     5161 atatgcaaac attttgtttt gagtaaaaat gtgtcaaatc gtggcctcta atgaccgaag
     5221 ttaatatgag gagtaaaaca ctagatcccc aaacaagctt gaaactgaag gcgggaaacg
     5281 acaatctgat catgagcgga gaattaaggg agtcacgtta tgacccccgc cgatgacgcg
     5341 ggacaagccg ttttacgttt ggaactgaca gaaccgcaac gttgaaggag ccactcagcc
     5401 gcgggtttct ggagtttaat gagctaagca catacgtcag aaaccattat tgcgcgttca
     5461 aaagtcgcct aaggtcacta tcagctagca aatatttctt gtcaaaaatg ctccactgac
     5521 gttccataaa ttcccctcgg tatccaatta gagtctcata ttcactctca atccaaataa
     5581 tctgcaccgg atccgctaga ggatctcgac ctgcaagatc ccggggggca atgagatatg
     5641 aaaaagcctg aactcaccgc gacgtctgtc gagaagtttc tgatcgaaaa gttcgacagc
     5701 gtctccgacc tgatgcagct ctcggagggc gaagaatctc gtgctttcag cttcgatata
     5761 ggagggcgtg gatatgtcct gcgggtaaat agctgcgccg atggtttcta caaagatcgt
     5821 tatgtttatc ggcactttgc atcggccgcg ctcccgattc cggaagtgct tgacattggg
     5881 gaattcagcg agagcctgac ctattgcatc tcccgccgtg cacagggtgt cacgttgcaa
     5941 gacctgcctg aaaccgaact gcccgctgtt ctgcagccgg tcgcggaggc catggatgcg
     6001 atcgctgcgg ccgatcttag ccagacgagc gggttcggcc cattcggacc gcaaggaatc
     6061 ggtcaataca ctacatggcg tgatttcata tgcgcgattg ctgatcccca tgtgtatcac
     6121 tggcaaactg tgatggacga caccgtcagt gcgtccgtcg cgcaggctct cgatgagctg
     6181 atgctttggg ccgaggactg ccccgaagtc cggcacctcg tgcacgcgga tttcggctcc
     6241 aacaatgtcc tgacggacaa tggccgcata acagcggtca ttgactggag cgaggcgatg
     6301 ttcggggatt cccaatacga ggtcgccaac atcttcttct ggaggccgtg gttggcttgt
     6361 atggagcagc agacgcgcta cttcgagcgg aggcatccgg agcttgcagg atcgccgcgg
     6421 ctccgggcgt atatgctccg cattggtctt gaccaactct atcagagctt ggttgacggc
     6481 aatttcgatg atgcagcttg ggcgcagggt cgatgcgacg caatcgtccg atccggagcc
     6541 gggactgtcg ggcgtacaca aatcgcccgc agaagcgcgg ccgtctggac cgatggctgt
     6601 gtagaagtac tcgccgatag tggaaaccga cgccccagca ctcgtccggg atcttggagg
     6661 tgatgtaaca tgatcacaag ctgatccccc gaatttcccc gatcgttcaa acatttggca
     6721 ataaagtttc ttaagattga atcctgttgc cggtcttgcg atgattatca tataatttct
     6781 gttgaattac gttaagcatg taataattaa catgtaatgc atgacgttat ttatgagatg
     6841 ggtttttatg attagagtcc cgcaattata catttaatac gcgatagaaa acaaaatata
     6901 gcgcgcaaac taggataaat tatcgcgcgc ggtgtcatct atgttactag atcgggaatt
     6961 gatcccccct cgacagcttg catgccagct tgggctgcag gtcgacgcgt caattgatat
     7021 cgatccaggc ctcccagctt tcgtccgtat catcggtttc gacaacgttc gtcaagttca
     7081 atgcatcagt ttcattgccc acacaccaga atcctactaa gtttgagtat tatggcattg
     7141 gaaaagctgt tttcttctat catttgttct gcttgtaatt tactgtgttc tttcagtttt
     7201 tgttttcgga catcaaaatg caaatggatg gataagagtt aataaatgat atggtccttt
     7261 tgttcattct caaattatta ttatctgttg tttttacttt aatgggttga atttaagtaa
     7321 gaaaggaact aacagtgtga tattaaggtg caatgttaga catataaaac agtctttcac
     7381 ctctctttgg ttatgtcttg aattggtttg tttcttcact tatctgtgta atcaagttta
     7441 ctatgagtct atgatcaagt aattatgcaa tcaagttaag tacagtatag gctttttgtg
     7501 tcgagggggt accgagtcga ggaattcact ggccgtcgtt ttacaacgtc gtgactggga
     7561 aaaccctggc gttacccaac ttaatcgcct tgcagcacat ccccctttcg ccagctggcg
     7621 taatagcgaa gaggcccgca ccgatcgccc ttcccaacag ttgcgcagcc tgaatggcgg
     7681 gtaccgagct cgaattcaat tcggcgttaa ttcagtacat taaaaacgtc cgcaatgtgt
     7741 tattaagttg tctaagcgtc aatttgttta caccacaata tatcctgcca ccagccagcc
     7801 aacagctccc cgaccggcag ctcggcacaa aatcaccact cgatacaggc agcccatcag
     7861 tccgggacgg cgtcagcggg agagccgttg taaggcggca gactttgctc atgttaccga
     7921 tgctattcgg aagaacggca actaagctgc cgggtttgaa acacggatga tctcgcggag
     7981 ggtagcatgt tgattgtaac gatgacagag cgttgctgcc tgtgatcaat tcgggcacga
     8041 acccagtgga cataagcctg ttcggttcgt aagctgtaat gcaagtagcg tatgcgctca
     8101 cgcaactggt ccagaacctt gaccgaacgc agcggtggta acggcgcagt ggcggttttc
     8161 atggcttgtt atgactgttt ttttggggta cagtctatgc ctcgggcatc caagcagcaa
     8221 gcgcgttacg ccgtgggtcg atgtttgatg ttatggagca gcaacgatgt tacgcagcag
     8281 ggcagtcgcc ctaaaacaaa gttaaacatc atgggggaag cggtgatcgc cgaagtatcg
     8341 actcaactat cagaggtagt tggcgtcatc gagcgccatc tcgaaccgac gttgctggcc
     8401 gtacatttgt acggctccgc agtggatggc ggcctgaagc cacacagtga tattgatttg
     8461 ctggttacgg tgaccgtaag gcttgatgaa acaacgcggc gagctttgat caacgacctt
     8521 ttggaaactt cggcttcccc tggagagagc gagattctcc gcgctgtaga agtcaccatt
     8581 gttgtgcacg acgacatcat tccgtggcgt tatccagcta agcgcgaact gcaatttgga
     8641 gaatggcagc gcaatgacat tcttgcaggt atcttcgagc cagccacgat cgacattgat
     8701 ctggctatct tgctgacaaa agcaagagaa catagcgttg ccttggtagg tccagcggcg
     8761 gaggaactct ttgatccggt tcctgaacag gatctatttg aggcgctaaa tgaaacctta
     8821 acgctatgga actcgccgcc cgactgggct ggcgatgagc gaaatgtagt gcttacgttg
     8881 tcccgcattt ggtacagcgc agtaaccggc aaaatcgcgc cgaaggatgt cgctgccgac
     8941 tgggcaatgg agcgcctgcc ggcccagtat cagcccgtca tacttgaagc tagacaggct
     9001 tatcttggac aagaagaaga tcgcttggcc tcgcgcgcag atcagttgga agaatttgtc
     9061 cactacgtga aaggcgagat caccaaggta gtcggcaaat aatgtctagc tagaaattcg
     9121 ttcaagccga cgccgcttcg cggcgcggct taactcaagc gttagatgca ctaagcacat
     9181 aattgctcac agccaaacta tcaggtcaag tctgctttta ttatttttaa gcgtgcataa
     9241 taagccctac acaaattggg agatatatca tgcatgacca aaatccctta acgtgagttt
     9301 tcgttccact gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg agatcctttt
     9361 tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt
     9421 ttgccggatc aagagctacc aactcttttt ccgaaggtaa ctggcttcag cagagcgcag
     9481 ataccaaata ctgtccttct agtgtagccg tagttaggcc accacttcaa gaactctgta
     9541 gcaccgccta catacctcgc tctgctaatc ctgttaccag tggctgctgc cagtggcgat
     9601 aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac cggataaggc gcagcggtcg
     9661 ggctgaacgg ggggttcgtg cacacagccc agcttggagc gaacgaccta caccgaactg
     9721 agatacctac agcgtgagct atgagaaagc gccacgcttc ccgaagggag aaaggcggac
     9781 aggtatccgg taagcggcag ggtcggaaca ggagagcgca cgagggagct tccaggggga
     9841 aacgcctggt atctttatag tcctgtcggg tttcgccacc tctgacttga gcgtcgattt
     9901 ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc ggccttttta
     9961 cggttcctgg ccttttgctg gccttttgct cacatgttct ttcctgcgtt atcccctgat
    10021 tctgtggata accgtattac cgcctttgag tgagctgata ccgctcgccg cagccgaacg
    10081 accgagcgca gcgagtcagt gagcgaggaa gcggaagagc gcctgatgcg gtattttctc
    10141 cttacgcatc tgtgcggtat ttcacaccgc atatggtgca ctctcagtac aatctgctct
    10201 gatgccgcat agttaagcca gtatacactc cgctatcgct acgtgactgg gtcatggctg
    10261 cgccccgaca cccgccaaca cccgctgacg cgccctgacg ggcttgtctg ctcccggcat
    10321 ccgcttacag acaagctgtg accgtctccg ggagctgcat gtgtcagagg ttttcaccgt
    10381 catcaccgaa acgcgcgagg cagggtgcct tgatgtgggc gccggcggtc gagtggcgac
    10441 ggcgcggctt gtccgcgccc tggtagattg cctggccgta ggccagccat ttttgagcgg
    10501 ccagcggccg cgataggccg acgcgaagcg gcggggcgta gggagcgcag cgaccgaagg
    10561 gtaggcgctt tttgcagctc ttcggctgtg cgctggccag acagttatgc acaggccagg
    10621 cgggttttaa gagttttaat aagttttaaa gagttttagg cggaaaaatc gccttttttc
    10681 tcttttatat cagtcactta catgtgtgac cggttcccaa tgtacggctt tgggttccca
    10741 atgtacgggt tccggttccc aatgtacggc tttgggttcc caatgtacgt gctatccaca
    10801 ggaaagagac cttttcgacc tttttcccct gctagggcaa tttgccctag catctgctcc
    10861 gtacattagg aaccggcgga tgcttcgccc tcgatcaggt tgcggtagcg catgactagg
    10921 atcgggccag cctgccccgc ctcctccttc aaatcgtact ccggcaggtc atttgacccg
    10981 atcagcttgc gcacggtgaa acagaacttc ttgaactctc cggcgctgcc actgcgttcg
    11041 tagatcgtct tgaacaacca tctggcttct gccttgcctg cggcgcggcg tgccaggcgg
    11101 tagagaaaac ggccgatgcc gggatcgatc aaaaagtaat cggggtgaac cgtcagcacg
    11161 tccgggttct tgccttctgt gatctcgcgg tacatccaat cagctagctc gatctcgatg
    11221 tactccggcc gcccggtttc gctctttacg atcttgtagc ggctaatcaa ggcttcaccc
    11281 tcggataccg tcaccaggcg gccgttcttg gccttcttcg tacgctgcat ggcaacgtgc
    11341 gtggtgttta accgaatgca ggtttctacc aggtcgtctt tctgctttcc gccatcggct
    11401 cgccggcaga acttgagtac gtccgcaacg tgtggacgga acacgcggcc gggcttgtct
    11461 cccttccctt cccggtatcg gttcatggat tcggttagat gggaaaccgc catcagtacc
    11521 aggtcgtaat cccacacact ggccatgccg gccggccctg cggaaacctc tacgtgcccg
    11581 tctggaagct cgtagcggat cacctcgcca gctcgtcggt cacgcttcga cagacggaaa
    11641 acggccacgt ccatgatgct gcgactatcg cgggtgccca cgtcatagag catcggaacg
    11701 aaaaaatctg gttgctcgtc gcccttgggc ggcttcctaa tcgacggcgc accggctgcc
    11761 ggcggttgcc gggattcttt gcggattcga tcagcggccg cttgccacga ttcaccgggg
    11821 cgtgcttctg cctcgatgcg ttgccgctgg gcggcctgcg cggccttcaa cttctccacc
    11881 aggtcatcac ccagcgccgc gccgatttgt accgggccgg atggtttgcg accgctcacg
    11941 ccgattcctc gggcttgggg gttccagtgc cattgcaggg ccggcagaca acccagccgc
    12001 ttacgcctgg ccaaccgccc gttcctccac acatggggca ttccacggcg tcggtgcctg
    12061 gttgttcttg attttccatg ccgcctcctt tagccgctaa aattcatcta ctcatttatt
    12121 catttgctca tttactctgg tagctgcgcg atgtattcag atagcagctc ggtaatggtc
    12181 ttgccttggc gtaccgcgta catcttcagc ttggtgtgat cctccgccgg caactgaaag
    12241 ttgacccgct tcatggctgg cgtgtctgcc aggctggcca acgttgcagc cttgctgctg
    12301 cgtgcgctcg gacggccggc acttagcgtg tttgtgcttt tgctcatttt ctctttacct
    12361 cattaactca aatgagtttt gatttaattt cagcggccag cgcctggacc tcgcgggcag
    12421 cgtcgccctc gggttctgat tcaagaacgg ttgtgccggc ggcggcagtg cctgggtagc
    12481 tcacgcgctg cgtgatacgg gactcaagaa tgggcagctc gtacccggcc agcgcctcgg
    12541 caacctcacc gccgatgcgc gtgcctttga tcgcccgcga cacgacaaag gccgcttgta
    12601 gccttccatc cgtgacctca atgcgctgct taaccagctc caccaggtcg gcggtggccc
    12661 atatgtcgta agggcttggc tgcaccggaa tcagcacgaa gtcggctgcc ttgatcgcgg
    12721 acacagccaa gtccgccgcc tggggcgctc cgtcgatcac tacgaagtcg cgccggccga
    12781 tggccttcac gtcgcggtca atcgtcgggc ggtcgatgcc gacaacggtt agcggttgat
    12841 cttcccgcac ggccgcccaa tcgcgggcac tgccctgggg atcggaatcg actaacagaa
    12901 catcggcccc ggcgagttgc agggcgcggg ctagatgggt tgcgatggtc gtcttgcctg
    12961 acccgccttt ctggttaagt acagcgataa ccttcatgcg ttccccttgc gtatttgttt
    13021 atttactcat cgcatcatat acgcagcgac cgcatgacgc aagctgtttt actcaaatac
    13081 acatcacctt tttagacggc ggcgctcggt ttcttcagcg gccaagctgg ccggccaggc
    13141 cgccagcttg gcatcagaca aaccggccag gatttcatgc agccgcacgg ttgagacgtg
    13201 cgcgggcggc tcgaacacgt acccggccgc gatcatctcc gcctcgatct cttcggtaat
    13261 gaaaaacggt tcgtcctggc cgtcctggtg cggtttcatg cttgttcctc ttggcgttca
    13321 ttctcggcgg ccgccagggc gtcggcctcg gtcaatgcgt cctcacggaa ggcaccgcgc
    13381 cgcctggcct cggtgggcgt cacttcctcg ctgcgctcaa gtgcgcggta cagggtcgag
    13441 cgatgcacgc caagcagtgc agccgcctct ttcacggtgc ggccttcctg gtcgatcagc
    13501 tcgcgggcgt gcgcgatctg tgccggggtg agggtagggc gggggccaaa cttcacgcct
    13561 cgggccttgg cggcctcgcg cccgctccgg gtgcggtcga tgattaggga acgctcgaac
    13621 tcggcaatgc cggcgaacac ggtcaacacc atgcggccgg ccggcgtggt ggtgtcggcc
    13681 cacggctctg ccaggctacg caggcccgcg ccggcctcct ggatgcgctc ggcaatgtcc
    13741 agtaggtcgc gggtgctgcg ggccaggcgg tctagcctgg tcactgtcac aacgtcgcca
    13801 gggcgtaggt ggtcaagcat cctggccagc tccgggcggt cgcgcctggt gccggtgatc
    13861 ttctcggaaa acagcttggt gcagccggcc gcgtgcagtt cggcccgttg gttggtcaag
    13921 tcctggtcgt cggtgctgac gcgggcatag cccagcaggc cagcggcggc gctcttgttc
    13981 atggcgtaat gtctccggtt ctagtcgcaa gtattctact ttatgcgact aaaacacgcg
    14041 acaagaaaac gccaggaaaa gggcagggcg gcagcctgtc gcgtaactta ggacttgtgc
    14101 gacatgtcgt tttcagaaga cggctgcact gaacgtcaga agccgactgc actatagcag
    14161 cggaggggtt ggatcaaagt actttaaagt actttaaagt actttaaagt actttgatcc
    14221 cgaggggaac cctgtggttg gcatgcacat acaaatggac gaacggataa accttttcac
    14281 gcccttttaa atatccgatt attctaataa acgctctttt ctcttaggtt taccc
//
