LOCUS       sequence-460068-        1770 bp    DNA     linear   UNA 15-APR-2025
DEFINITION  Mammalian expression of Chimeric Higher-Order Assemblies for 
            Receptor Mediated Signaling (CHARMS): CHARMS-TIR.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1770)
  AUTHORS   Lichtenstein MA, Cao F, Lobnow F, Dirvanskyte P, Kulesza A, Ziska E,
            Taylor MJ
  TITLE     Bottom-up reconstruction of functional Death Domain Signalosomes 
            reveals a requirement for polymer stability and avidity
  JOURNAL   bioRxiv 2024.05.08.593169
REFERENCE   2  (bases 1 to 1770)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "bioRxiv 
            2024.05.08.593169"
COMMENT     Sequence Label: pHRSV_CHARMS-TIR
FEATURES             Location/Qualifiers
     source          1..1770
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             join(1054..1056,1057..1770)
                     /codon_start=1
                     /product="enhanced GFP with monomerizing A206K mutation 
                     (Zacharias et al., 2002)"
                     /label=mEGFP
                     /note="mammalian codon-optimized"
                     /translation="VSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLLE
                     FVTAAGITLGMDELYK"
     primer_bind     complement(1096..1117)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(1357..1376)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     1704..1725
                     /label=EGFP-C
                     /note="EGFP, forward primer"
ORIGIN
        1 atgccacaaa caaaggaact gggaggcatc accacccttg atgaccccct aggacaaacg
       61 ccggaacttt tcgatgcctt tatctgctac tgccccaacg atatcgagtt tgtgcaggag
      121 atgatccggc aactagaaca gacagactat cggcttaagt tgtgtgtgtc cgaccgtgac
      181 gtcctgccgg gcacctgtgt ctggtccatt gccagcgagc taattgagaa aaggtgtcgc
      241 cgcatggtgg tggttgtttc tgacgattat ctacagagca aggaatgtga cttccagacc
      301 aagtttgcac tcagcctgtc tccaggtgtc caacagaagc gactgattcc tattaaatac
      361 aaggcgatga agaaggactt tcccagtatc ctgcggttca tcactatatg cgactatacc
      421 aacccttgca ccaagtcctg gttctggacc cgccttgcca aggctttgtc cctgcccgga
      481 ggtagtggcg ggtcccctcc ttctccccaa gaaaactcct atgtcagctc cactggtagg
      541 gctcactccg gagccgcacc atggcagcca ctcgctgcac catcaggtgc ttcagcccag
      601 gccgcagagc aactgcaacg gggacccaac cagccagtcg agtccgacga aagtcttgga
      661 gggctgagcg ctgctctgcg gtcctggcac cttacaccaa gttgtcccct tgatcctgct
      721 cctctgcggg aggctgggtg cccccaaggt gacacagcag gggaatcctc atggggttca
      781 ggacctggga gccggccaac cgctgttgaa ggcctggctt tgggctcctc tgcatcatca
      841 tcatcagaac ctccccagat tatcatcaat ccagctcgcc agaaaatggt tcagaaattg
      901 gctttgtatg aggatggtgc cctggattcc ttgcaattgc tgtcatcttc ttctcttccc
      961 ggacttggac ttgaacaaga cagacaaggt ccagaggagt ccgacgagtt ccagagcgga
     1021 ggttccgggg gtactggggg tagtggagga agtgtgagca agggcgagga gctgttcacc
     1081 ggggtggtgc ccatcctggt cgagctggac ggcgacgtaa acggccacaa gttcagcgtg
     1141 tccggcgagg gcgagggcga tgccacctac ggcaagctga ccctgaagtt catctgcacc
     1201 accggcaagc tgcccgtgcc ctggcccacc ctcgtgacca ccctgaccta cggcgtgcag
     1261 tgcttcagcc gctaccccga ccacatgaag cagcacgact tcttcaagtc cgccatgccc
     1321 gaaggctacg tccaggagcg caccatcttc ttcaaggacg acggcaacta caagacccgc
     1381 gccgaggtga agttcgaggg cgacaccctg gtgaaccgca tcgagctgaa gggcatcgac
     1441 ttcaaggagg acggcaacat cctggggcac aagctggagt acaactacaa cagccacaac
     1501 gtctatatca tggccgacaa gcagaagaac ggcatcaagg tgaacttcaa gatccgccac
     1561 aacatcgagg acggcagcgt gcagctcgcc gaccactacc agcagaacac ccccatcggc
     1621 gacggccccg tgctgctgcc cgacaaccac tacctgagca cccagtccaa gctgagcaaa
     1681 gaccccaacg agaagcgcga tcacatggtc ctgctggagt tcgtgaccgc cgccgggatc
     1741 actctcggca tggacgagct gtacaagtaa
//
