LOCUS       sequence-460110-       10533 bp    DNA     linear   UNA 15-APR-2025
DEFINITION  Mammalian expression of Chimeric Higher-Order Assemblies for 
            Receptor Mediated Signaling (CHARMS) variant: CHARMS three mutations
            in the death domain that disrupt oligomer formation.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 10533)
  AUTHORS   Lichtenstein MA, Cao F, Lobnow F, Dirvanskyte P, Kulesza A, Ziska E,
            Taylor MJ
  TITLE     Bottom-up reconstruction of functional Death Domain Signalosomes 
            reveals a requirement for polymer stability and avidity
  JOURNAL   bioRxiv 2024.05.08.593169
REFERENCE   2  (bases 1 to 10533)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "bioRxiv 
            2024.05.08.593169"
COMMENT     Sequence Label: pHRSV_CHARMS-V43D/E52K/R62E
FEATURES             Location/Qualifiers
     source          1..10533
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        1..211
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      70..205
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             join(1391..1393,1394..2107)
                     /codon_start=1
                     /product="enhanced GFP with monomerizing A206K mutation 
                     (Zacharias et al., 2002)"
                     /label=mEGFP
                     /note="mammalian codon-optimized"
                     /translation="VSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLLE
                     FVTAAGITLGMDELYK"
     primer_bind     complement(1433..1454)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(1694..1713)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     2041..2062
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     misc_feature    2168..2756
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(2221..2241)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(2639..2650)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(2759..2775)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2760..2776)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     LTR             2866..3099
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     promoter        complement(3124..3142)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(3125..3142)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(3432..3451)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     primer_bind     complement(3665..3684)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     3784..3806
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(3844..3862)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        3930..4034
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(4035..4103,4104..4895)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(4253..4272)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      5066..5654
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     5555..5574
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     5808..5825
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     complement(5895..5915)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        5900..6229
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      6080..6215
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     intron          7363..7428
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             7558..7578
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    8003..8137
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(8040..8059)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     8094..8113
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     LTR             8305..8938
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    8985..9110
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    9607..9840
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             10025..10069
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    10354..10471
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
ORIGIN
        1 gcaggcagaa gtatgcaaag catgcatctc aattagtcag caaccatagt cccgccccta
       61 actccgccca tcccgcccct aactccgccc agttccgccc attctccgcc ccatggctga
      121 ctaatttttt ttatttatgc agaggccgag gccgcctctg cctctgagct attccagaag
      181 tagtgaggag gcttttttgg aggcctaggc tacgcgtgcc accatgccac aaacaaagga
      241 actgggaggc atcaccaccc ttgatgaccc cctaggacaa acgccggaac ttttcgatgc
      301 ctttatctgc tactgcccca acgatatcga gtttgtgcag gagatgatcc ggcaactaga
      361 acagacagac tatcggctta agttgtgtgt gtccgaccgt gacgtcctgc cgggcacctg
      421 tgtctggtcc attgccagcg agctaattga gaaaaggtgt cgccgcatgg tggtggttgt
      481 ttctgacgat tatctacaga gcaaggaatg tgacttccag accaagtttg cactcagcct
      541 gtctccaggt gtccaacaga agcgactgat tcctattaaa tacaaggcga tgaagaagga
      601 ctttcccagt atcctgcggt tcatcactat atgcgactat accaaccctt gcaccaagtc
      661 ctggttctgg acccgccttg ccaaggcttt gtccctgccc ggaggtagtg gcgggtcccc
      721 tccttctccc caagaaaact cctatgtcag ctccactggt agggctcact ccggagccgc
      781 accatggcag ccactcgctg caccatcagg tgcttcagcc caggccgcag agcaactgca
      841 acggggaccc aaccagccag tcgagtccga cgaaagtctt ggagggctga gcgctgctct
      901 gcggtcctgg caccttacac caagttgtcc ccttgatcct gctcctctgc gggaggctgg
      961 gtgcccccaa ggtgacacag caggggaatc ctcatggggt tcaggacctg ggagccggcc
     1021 aaccgctgtt gaaggcctgg ctttgggctc ctctgcatca tcatcatcag aacctcccca
     1081 gattatcatc aatccagctc gccagaaaat ggttcagaaa ttggctttgt atgaggatgg
     1141 tgccctggat tccttgcaat tgctgtcatc ttcttctctt cccggacttg gacttgaaca
     1201 agacagacaa ggtccagagg agtccgacga gttccagggt ggcagtccta agcaggaacc
     1261 tcaagaaatc gacttcggtg ggggctcagg tggaactggg tcaggtggcg gttctggagg
     1321 tccaaaacaa gaaccacagg agatagactt tagcggaggt tccgggggta ctgggggtag
     1381 tggaggaagt gtgagcaagg gcgaggagct gttcaccggg gtggtgccca tcctggtcga
     1441 gctggacggc gacgtaaacg gccacaagtt cagcgtgtcc ggcgagggcg agggcgatgc
     1501 cacctacggc aagctgaccc tgaagttcat ctgcaccacc ggcaagctgc ccgtgccctg
     1561 gcccaccctc gtgaccaccc tgacctacgg cgtgcagtgc ttcagccgct accccgacca
     1621 catgaagcag cacgacttct tcaagtccgc catgcccgaa ggctacgtcc aggagcgcac
     1681 catcttcttc aaggacgacg gcaactacaa gacccgcgcc gaggtgaagt tcgagggcga
     1741 caccctggtg aaccgcatcg agctgaaggg catcgacttc aaggaggacg gcaacatcct
     1801 ggggcacaag ctggagtaca actacaacag ccacaacgtc tatatcatgg ccgacaagca
     1861 gaagaacggc atcaaggtga acttcaagat ccgccacaac atcgaggacg gcagcgtgca
     1921 gctcgccgac cactaccagc agaacacccc catcggcgac ggccccgtgc tgctgcccga
     1981 caaccactac ctgagcaccc agtccaagct gagcaaagac cccaacgaga agcgcgatca
     2041 catggtcctg ctggagttcg tgaccgccgc cgggatcact ctcggcatgg acgagctgta
     2101 caagtaaagc ggccgcgact ctagagtcga cctgcaggca tgcaagcttg atatcaagct
     2161 tatcgataat caacctctgg attacaaaat ttgtgaaaga ttgactggta ttcttaacta
     2221 tgttgctcct tttacgctat gtggatacgc tgctttaatg cctttgtatc atgctattgc
     2281 ttcccgtatg gctttcattt tctcctcctt gtataaatcc tggttgctgt ctctttatga
     2341 ggagttgtgg cccgttgtca ggcaacgtgg cgtggtgtgc actgtgtttg ctgacgcaac
     2401 ccccactggt tggggcattg ccaccacctg tcagctcctt tccgggactt tcgctttccc
     2461 cctccctatt gccacggcgg aactcatcgc cgcctgcctt gcccgctgct ggacaggggc
     2521 tcggctgttg ggcactgaca attccgtggt gttgtcgggg aaatcatcgt cctttccttg
     2581 gctgctcgcc tgtgttgcca cctggattct gcgcgggacg tccttctgct acgtcccttc
     2641 ggccctcaat ccagcggacc ttccttcccg cggcctgctg ccggctctgc ggcctcttcc
     2701 gcgtcttcgc cttcgccctc agacgagtcg gatctccctt tgggccgcct ccccgcatcg
     2761 ataccgtcga cctcgaggga attaattcga gctcggtacc tttaagacca atgacttaca
     2821 aggcagctgt agatcttagc cactttttaa aagaaaaggg gggactggaa gggctaattc
     2881 actcccaacg aagacaagat ctgctttttg cttgtactgg gtctctctgg ttagaccaga
     2941 tctgagcctg ggagctctct ggctaactag ggaacccact gcttaagcct caataaagct
     3001 tgccttgagt gcttcaagta gtgtgtgccc gtctgttgtg tgactctggt aactagagat
     3061 ccctcagacc cttttagtca gtgtggaaaa tctctagcag catctagaat taattccgtg
     3121 tattctatag tgtcacctaa atcgtatgtg tatgatacat aaggttatgt attaattgta
     3181 gccgcgttct aacgacaata tgtacaagcc taattgtgta gcatctggct tactgaagca
     3241 gaccctatca tctctctcgt aaactgccgt cagagtcggt ttggttggac gaaccttctg
     3301 agtttctggt aacgccgtcc cgcacccgga aatggtcagc gaaccaatca gcagggtcat
     3361 cgctagccag atcctctacg ccggacgcat cgtggccggc atcaccggcg ccacaggtgc
     3421 ggttgctggc gcctatatcg ccgacatcac cgatggggaa gatcgggctc gccacttcgg
     3481 gctcatgagc gcttgtttcg gcgtgggtat ggtggcaggc cccgtggccg ggggactgtt
     3541 gggcgccatc tccttgcatg caccattcct tgcggcggcg gtgctcaacg gcctcaacct
     3601 actactgggc tgcttcctaa tgcaggagtc gcataaggga gagcgtcgaa tggtgcactc
     3661 tcagtacaat ctgctctgat gccgcatagt taagccagcc ccgacacccg ccaacacccg
     3721 ctgacgcgcc ctgacgggct tgtctgctcc cggcatccgc ttacagacaa gctgtgaccg
     3781 tctccgggag ctgcatgtgt cagaggtttt caccgtcatc accgaaacgc gcgagacgaa
     3841 agggcctcgt gatacgccta tttttatagg ttaatgtcat gataataatg gtttcttaga
     3901 cgtcaggtgg cacttttcgg ggaaatgtgc gcggaacccc tatttgttta tttttctaaa
     3961 tacattcaaa tatgtatccg ctcatgagac aataaccctg ataaatgctt caataatatt
     4021 gaaaaaggaa gagtatgagt attcaacatt tccgtgtcgc ccttattccc ttttttgcgg
     4081 cattttgcct tcctgttttt gctcacccag aaacgctggt gaaagtaaaa gatgctgaag
     4141 atcagttggg tgcacgagtg ggttacatcg aactggatct caacagcggt aagatccttg
     4201 agagttttcg ccccgaagaa cgttttccaa tgatgagcac ttttaaagtt ctgctatgtg
     4261 gcgcggtatt atcccgtatt gacgccgggc aagagcaact cggtcgccgc atacactatt
     4321 ctcagaatga cttggttgag tactcaccag tcacagaaaa gcatcttacg gatggcatga
     4381 cagtaagaga attatgcagt gctgccataa ccatgagtga taacactgcg gccaacttac
     4441 ttctgacaac gatcggagga ccgaaggagc taaccgcttt tttgcacaac atgggggatc
     4501 atgtaactcg ccttgatcgt tgggaaccgg agctgaatga agccatacca aacgacgagc
     4561 gtgacaccac gatgcctgta gcaatggcaa caacgttgcg caaactatta actggcgaac
     4621 tacttactct agcttcccgg caacaattaa tagactggat ggaggcggat aaagttgcag
     4681 gaccacttct gcgctcggcc cttccggctg gctggtttat tgctgataaa tctggagccg
     4741 gtgagcgtgg gtctcgcggt atcattgcag cactggggcc agatggtaag ccctcccgta
     4801 tcgtagttat ctacacgacg gggagtcagg caactatgga tgaacgaaat agacagatcg
     4861 ctgagatagg tgcctcactg attaagcatt ggtaactgtc agaccaagtt tactcatata
     4921 tactttagat tgatttaaaa cttcattttt aatttaaaag gatctaggtg aagatccttt
     4981 ttgataatct catgaccaaa atcccttaac gtgagttttc gttccactga gcgtcagacc
     5041 ccgtagaaaa gatcaaagga tcttcttgag atcctttttt tctgcgcgta atctgctgct
     5101 tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa gagctaccaa
     5161 ctctttttcc gaaggtaact ggcttcagca gagcgcagat accaaatact gttcttctag
     5221 tgtagccgta gttaggccac cacttcaaga actctgtagc accgcctaca tacctcgctc
     5281 tgctaatcct gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt accgggttgg
     5341 actcaagacg atagttaccg gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca
     5401 cacagcccag cttggagcga acgacctaca ccgaactgag atacctacag cgtgagctat
     5461 gagaaagcgc cacgcttccc gaagggagaa aggcggacag gtatccggta agcggcaggg
     5521 tcggaacagg agagcgcacg agggagcttc cagggggaaa cgcctggtat ctttatagtc
     5581 ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt gtgatgctcg tcaggggggc
     5641 ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc ttttgctggc
     5701 cttttgctca catgttcttt cctgcgttat cccctgattc tgtggataac cgtattaccg
     5761 cctttgagtg agctgatacc gctcgccgca gccgaacgac cgagcgcagc gagtcagtga
     5821 gcgaggaagc ggaagagcgc ccaatacgca aaccgcctct ccccgcgcgt tggccgattc
     5881 attaatgcag ctgtggaatg tgtgtcagtt agggtgtgga aagtccccag gctccccagc
     5941 aggcagaagt atgcaaagca tgcatctcaa ttagtcagca accaggtgtg gaaagtcccc
     6001 aggctcccca gcaggcagaa gtatgcaaag catgcatctc aattagtcag caaccatagt
     6061 cccgccccta actccgccca tcccgcccct aactccgccc agttccgccc attctccgcc
     6121 ccatggctga ctaatttttt ttatttatgc agaggccgag gccgcctcgg cctctgagct
     6181 attccagaag tagtgaggag gcttttttgg aggcctaggc ttttgcaaaa agcttggaca
     6241 caagacaggc ttgcgagata tgtttgagaa taccacttta tcccgcgtca gggagaggca
     6301 gtgcgtaaaa agacgcggac tcatgtgaaa tactggtttt tagtgcgcca gatctctata
     6361 atctcgcgca acctattttc ccctcgaaca ctttttaagc cgtagataaa caggctggga
     6421 cacttcacat gagcgaaaaa tacatcgtca cctgggacat gttgcagatc catgcacgta
     6481 aactcgcaag ccgactgatg ccttctgaac aatggaaagg cattattgcc gtaagccgtg
     6541 gcggtctgta ccgggtgcgt tactggcgcg tgaactgggt attcgtcatg tcgataccgt
     6601 ttgtatttcc agctacgatc acgacaacca gcgcgagctt aaagtgctga aacgcgcaga
     6661 aggcgatggc gaaggcttca tcgttattga tgacctggtg gataccggtg gtactgcggt
     6721 tgcgattcgt gaaatgtatc caaaagcgca ctttgtcacc atcttcgcaa aaccggctgg
     6781 tcgtccgctg gttgatgact atgttgttga tatcccgcaa gatacctgga ttgaacagcc
     6841 gtgggatatg ggcgtcgtat tcgtcccgcc aatctccggt cgctaatctt ttcaacgcct
     6901 ggcactgccg ggcgttgttc tttttaactt caggcgggtt acaatagttt ccagtaagta
     6961 ttctggaggc tgcatccatg acacaggcaa acctgagcga aaccctgttc aaaccccgct
     7021 ttaaacatcc tgaaacctcg acgctagtcc gccgctttaa tcacggcgca caaccgcctg
     7081 tgcagtcggc ccttgatggt aaaaccatcc ctcactggta tcgcatgatt aaccgtctga
     7141 tgtggatctg gcgcggcatt gacccacgcg aaatcctcga cgtccaggca cgtattgtga
     7201 tgagcgatgc cgaacgtacc gacgatgatt tatacgatac ggtgattggc taccgtggcg
     7261 gcaactggat ttatgagtgg gccccggatc tttgtgaagg aaccttactt ctgtggtgtg
     7321 acataattgg acaaactacc tacagagatt taaagctcta aggtaaatat aaaattttta
     7381 agtgtataat gtgttaaact actgattcta attgtttgtg tattttagat tccaacctat
     7441 ggaactgatg aatgggagca gtggtggaat gcctttaatg aggaaaacct gttttgctca
     7501 gaagaaatgc catctagtga tgatgaggct actgctgact ctcaacattc tactcctcca
     7561 aaaaagaaga gaaaggtaga agaccccaag gactttcctt cagaattgct aagttttttg
     7621 agtcatgctg tgtttagtaa tagaactctt gcttgctttg ctatttacac cacaaaggaa
     7681 aaagctgcac tgctatacaa gaaaattatg gaaaaatatt ctgtaacctt tataagtagg
     7741 cataacagtt ataatcataa catactgttt tttcttactc cacacaggca tagagtgtct
     7801 gctattaata actatgctca aaaattgtgt acctttagct ttttaatttg taaaggggtt
     7861 aataaggaat atttgatgta tagtgccttg actagagatc ataatcagcc ataccacatt
     7921 tgtagaggtt ttacttgctt taaaaaacct cccacacctc cccctgaacc tgaaacataa
     7981 aatgaatgca attgttgttg ttaacttgtt tattgcagct tataatggtt acaaataaag
     8041 caatagcatc acaaatttca caaataaagc atttttttca ctgcattcta gttgtggttt
     8101 gtccaaactc atcaatgtat cttatcatgt ctggatcaac tggataactc aagctaacca
     8161 aaatcatccc aaacttccca ccccataccc tattaccact gccaattacc tgtggtttca
     8221 tttactctaa acctgtgatt cctctgaatt attttcattt taaagaaatt gtatttgtta
     8281 aatatgtact acaaacttag tagttggaag ggctaattca ctcccaaaga agacaagata
     8341 tccttgatct gtggatctac cacacacaag gctacttccc tgattagcag aactacacac
     8401 cagggccagg ggtcagatat ccactgacct ttggatggtg ctacaagcta gtaccagttg
     8461 agccagataa ggtagaagag gccaataaag gagagaacac cagcttgtta caccctgtga
     8521 gcctgcatgg gatggatgac ccggagagag aagtgttaga gtggaggttt gacagccgcc
     8581 tagcatttca tcacgtggcc cgagagctgc atccggagta cttcaagaac tgctgatatc
     8641 gagcttgcta caagggactt tccgctgggg actttccagg gaggcgtggc ctgggcggga
     8701 ctggggagtg gcgagccctc agatcctgca tataagcagc tgctttttgc ctgtactggg
     8761 tctctctggt tagaccagat ctgagcctgg gagctctctg gctaactagg gaacccactg
     8821 cttaagcctc aataaagctt gccttgagtg cttcaagtag tgtgtgcccg tctgttgtgt
     8881 gactctggta actagagatc cctcagaccc ttttagtcag tgtggaaaat ctctagcagt
     8941 ggcgcccgaa cagggacttg aaagcgaaag ggaaaccaga ggagctctct cgacgcagga
     9001 ctcggcttgc tgaagcgcgc acggcaagag gcgaggggcg gcgactggtg agtacgccaa
     9061 aaattttgac tagcggaggc tagaaggaga gagatgggtg cgagagcgtc agtattaagc
     9121 gggggagaat tagatcgcga tgggaaaaaa ttcggttaag gccaggggga aagaaaaaat
     9181 ataaattaaa acatatagta tgggcaagca gggagctaga acgattcgca gttaatcctg
     9241 gcctgttaga aacatcagaa ggctgtagac aaatactggg acagctacaa ccatcccttc
     9301 agacaggatc agaagaactt agatcattat ataatacagt agcaaccctc tattgtgtgc
     9361 atcaaaggat agagataaaa gacaccaagg aagctttaga caagatagag gaagagcaaa
     9421 acaaaagtaa gaccaccgca cagcaagcgg ccggccgctg atcttcagac ctggaggagg
     9481 agatatgagg gacaattgga gaagtgaatt atataaatat aaagtagtaa aaattgaacc
     9541 attaggagta gcacccacca aggcaaagag aagagtggtg cagagagaaa aaagagcagt
     9601 gggaatagga gctttgttcc ttgggttctt gggagcagca ggaagcacta tgggcgcagc
     9661 gtcaatgacg ctgacggtac aggccagaca attattgtct ggtatagtgc agcagcagaa
     9721 caatttgctg agggctattg aggcgcaaca gcatctgttg caactcacag tctggggcat
     9781 caagcagctc caggcaagaa tcctggctgt ggaaagatac ctaaaggatc aacagctcct
     9841 ggggatttgg ggttgctctg gaaaactcat ttgcaccact gctgtgcctt ggaatgctag
     9901 ttggagtaat aaatctctgg aacagatttg gaatcacacg acctggatgg agtgggacag
     9961 agaaattaac aattacacaa gcttaataca ctccttaatt gaagaatcgc aaaaccagca
    10021 agaaaagaat gaacaagaat tattggaatt agataaatgg gcaagtttgt ggaattggtt
    10081 taacataaca aattggctgt ggtatataaa attattcata atgatagtag gaggcttggt
    10141 aggtttaaga atagtttttg ctgtactttc tatagtgaat agagttaggc agggatattc
    10201 accattatcg tttcagaccc acctcccaac cccgagggga cccgacaggc ccgaaggaat
    10261 agaagaagaa ggtggagaga gagacagaga cagatccatt cgattagtga acggatctcg
    10321 acggtatcgc caaatggcag tattcatcca caattttaaa agaaaagggg ggattggggg
    10381 gtacagtgca ggggaaagaa tagtagacat aatagcaaca gacatacaaa ctaaagaatt
    10441 acaaaaacaa attacaaaaa ttcaaaattt tcgggtttat tacagggaca gcagagatcc
    10501 agtttggatc gataagcttg atatcgaatt cag
//
