LOCUS       sequence-460249-         632 bp    DNA     linear   UNA 15-APR-2025
DEFINITION  Bacterial expression of N-terminally 6His-tagged MAX; includes TEV 
            cleavage site.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 632)
  AUTHORS   Yang J, Chung CI, Koach J, Liu H, Navalkar A, He H, Ma Z, Zhao Q, 
            Yang X, He L, Mittag T, Shen Y, Weiss WA, Shu X
  TITLE     MYC phase separation selectively modulates the transcriptome.
  JOURNAL   Nat Struct Mol Biol. 2024 Oct;31(10):1567-1579. doi: 
            10.1038/s41594-024-01322-6. Epub 2024 May 29.
  PUBMED    38811792
REFERENCE   2  (bases 1 to 632)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/s41594-024-01322-6"; journalName: "Nat Struct Mol Biol"; 
            date: "2024-10"; volume: "31"; issue: "10"; pages: "1567-1579"
COMMENT     Sequence Label: pDest17_MAX
FEATURES             Location/Qualifiers
     source          1..632
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    37..61
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             64..84
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     protein_bind    complement(572..596)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 ttgcaacaaa ttgatgagca atgctttttt ataatgccaa ctttgtacaa aaaagcaggc
       61 ttcgagaacc tgtattttca gggtagcatg agcgataacg atgatattga agtggaaagc
      121 gacgaagaac agcctcgttt tcagagcgca gcagataaac gtgcacatca taatgcactg
      181 gaacgtaaac gtcgcgatca tatcaaagat agctttcata gcctgcgtga tagcgttccg
      241 agcctgcagg gtgaaaaagc aagccgtgca cagattctgg ataaagcaac cgagtatatt
      301 cagtacatgc gtcgcaaaaa tcatacccat cagcaggata ttgatgatct gaaacgtcag
      361 aatgcactgc tggaacagca ggttcgtgcc ctggaaaaag cacgtagcag cgcacagctg
      421 cagaccaatt atccgagcag cgataatagc ctgtatacca atgcaaaagg tagcaccatt
      481 agcgcatttg atggtggtag cgatagcagc agcgaaagcg aaccggaaga accgcagagc
      541 cgtaaaaagc tgcgtatgga agcaagctaa gacccagctt tcttgtacaa agttggcatt
      601 ataagaaagc attgcttatc aatttgttgc aa
//
