LOCUS       sequence-460298-       10135 bp    DNA     circular SYN 15-APR-2025
DEFINITION  Expression of NIT-9 with Gephyrin linkage region in N. crassa at the
            his-3 locus.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10135)
  TITLE     Convergent Evolution Links Molybdenum Insertase Domains with 
            Organism-Specific Sequences.
REFERENCE   2  (bases 1 to 10135)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pCCG_nit9fl_Gephyrin_linker
FEATURES             Location/Qualifiers
     source          1..10135
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(98..117)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     217..239
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(277..295)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        363..467
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(468..536,537..1328)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(686..705)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      1499..2087
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     1988..2007
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     2241..2258
                     /label=L4440
                     /note="L4440 vector, forward primer"
     promoter        2345..2363
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             2638..2649
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     promoter        4007..4025
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     4979..4995
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     4979..4995
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     primer_bind     complement(7416..7432)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7417..7433)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     promoter        complement(join(10134..10135,1..17))
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(join(10135,1..17))
                     /label=SP6
                     /note="SP6 promoter, forward primer"
ORIGIN
        1 tatagtgtca cctaaatcgt atgtgtatga tacataaggt tatgtattaa ttgtagccgc
       61 gttctaacga caatatgtcc atatggtgca ctctcagtac aatctgctct gatgccgcat
      121 agttaagcca gccccgacac ccgccaacac ccgctgacgc gccctgacgg gcttgtctgc
      181 tcccggcatc cgcttacaga caagctgtga ccgtctccgg gagctgcatg tgtcagaggt
      241 tttcaccgtc atcaccgaaa cgcgcgagac gaaagggcct cgtgatacgc ctatttttat
      301 aggttaatgt catgataata atggtttctt agacgtcagg tggcactttt cggggaaatg
      361 tgcgcggaac ccctatttgt ttatttttct aaatacattc aaatatgtat ccgctcatga
      421 gacaataacc ctgataaatg cttcaataat attgaaaaag gaagagtatg agtattcaac
      481 atttccgtgt cgcccttatt cccttttttg cggcattttg ccttcctgtt tttgctcacc
      541 cagaaacgct ggtgaaagta aaagatgctg aagatcagtt gggtgcacga gtgggttaca
      601 tcgaactgga tctcaacagc ggtaagatcc ttgagagttt tcgccccgaa gaacgttttc
      661 caatgatgag cacttttaaa gttctgctat gtggcgcggt attatcccgt attgacgccg
      721 ggcaagagca actcggtcgc cgcatacact attctcagaa tgacttggtt gagtactcac
      781 cagtcacaga aaagcatctt acggatggca tgacagtaag agaattatgc agtgctgcca
      841 taaccatgag tgataacact gcggccaact tacttctgac aacgatcgga ggaccgaagg
      901 agctaaccgc ttttttgcac aacatggggg atcatgtaac tcgccttgat cgttgggaac
      961 cggagctgaa tgaagccata ccaaacgacg agcgtgacac cacgatgcct gtagcaatgg
     1021 caacaacgtt gcgcaaacta ttaactggcg aactacttac tctagcttcc cggcaacaat
     1081 taatagactg gatggaggcg gataaagttg caggaccact tctgcgctcg gcccttccgg
     1141 ctggctggtt tattgctgat aaatctggag ccggtgagcg tgggtctcgc ggtatcattg
     1201 cagcactggg gccagatggt aagccctccc gtatcgtagt tatctacacg acggggagtc
     1261 aggcaactat ggatgaacga aatagacaga tcgctgagat aggtgcctca ctgattaagc
     1321 attggtaact gtcagaccaa gtttactcat atatacttta gattgattta aaacttcatt
     1381 tttaatttaa aaggatctag gtgaagatcc tttttgataa tctcatgacc aaaatccctt
     1441 aacgtgagtt ttcgttccac tgagcgtcag accccgtaga aaagatcaaa ggatcttctt
     1501 gagatccttt ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag
     1561 cggtggtttg tttgccggat caagagctac caactctttt tccgaaggta actggcttca
     1621 gcagagcgca gataccaaat actgttcttc tagtgtagcc gtagttaggc caccacttca
     1681 agaactctgt agcaccgcct acatacctcg ctctgctaat cctgttacca gtggctgctg
     1741 ccagtggcga taagtcgtgt cttaccgggt tggactcaag acgatagtta ccggataagg
     1801 cgcagcggtc gggctgaacg gggggttcgt gcacacagcc cagcttggag cgaacgacct
     1861 acaccgaact gagataccta cagcgtgagc tatgagaaag cgccacgctt cccgaaggga
     1921 gaaaggcgga caggtatccg gtaagcggca gggtcggaac aggagagcgc acgagggagc
     1981 ttccaggggg aaacgcctgg tatctttata gtcctgtcgg gtttcgccac ctctgacttg
     2041 agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac gccagcaacg
     2101 cggccttttt acggttcctg gccttttgct ggccttttgc tcacatgttc tttcctgcgt
     2161 tatcccctga ttctgtggat aaccgtatta ccgcctttga gtgagctgat accgctcgcc
     2221 gcagccgaac gaccgagcgc agcgagtcag tgagcgagga agcggaagag cgcccaatac
     2281 gcaaaccgcc tctccccgcg cgttggccga ttcattaatg caggttaacc tggcttatcg
     2341 aaattaatac gactcactat agggagaccg gcagatccaa tgcggatgga ttcgctcact
     2401 cggtgccgcg gcggtgcgaa gttcctgccg tggattgggg gattgcacag acggtggagt
     2461 gcttgctaca gctaggtacc cagttgcaca ccaaacggtg cccctgtgcc cctgcgtcta
     2521 ggtgtgtcct gtgagcaact gcgcccgcgc gcggcaccgg gaagctgcgg gaacctcaat
     2581 ggatgattgg cgccagatcc ccggcatcac ctcagcacat gggcagcccg agaaggaatc
     2641 gagggtagaa gatattccag ggtttccgct agcacaactt ccaagcgcct ctaccgctac
     2701 tccacacacc cacgctgaat ggcacaacca ctgaggccta gcggtcccag ctccccgtaa
     2761 ccacgcagcc ccagcgaccg aggtcatcat gacactatga cgacaatctg gcgggagact
     2821 tgggaaaatc gatgacgggc acctaccttg ggaacaatag agcaagtcca gtcaacgccc
     2881 tagggaaggg ggaaaaaaaa aaaagagccc attctcacaa ggagcaacac tgtgctgcta
     2941 tcatagtaca ctacaagcaa agtcgggatg ttgctagaat ccgtcggcta cgtcggtaga
     3001 tacctcccat aggcagtaag tctaggtatc caatttatag ttccgcgtat ttcttcgtcg
     3061 aacggggcac agcaaagcct aacagccgca gtgcctcgca accaacacca agcagtaatc
     3121 tcatccatcc atacagatcc tcccacaata tctttacatg cgcacttgcg ctgtgttagc
     3181 agtttaggtt ccactaccaa ggctgacacg agcagcactg acagcaaact gctgcttggg
     3241 gccaagaaat tggcagtttg ctgagcacct acatatcagt acacatcaac cctggtttaa
     3301 ttgttagcct ggtactgtta accgtcatga acgaggacgt ggaggaagaa ccacgctaac
     3361 aattgaacca gggttgatgt atacctctct taccatattt gcttgcttcc acgtccctat
     3421 cctcaagcga ttttgttctt gactttttgt atttcaacgc aaaggccatt caggtttctc
     3481 atgttcaacc ctttggatgg cgatgccgtt cgtcccagga cctcgtgacc tttgaaagac
     3541 gagaaatata tcccaaagca acttctgctt atggacaagc aactgcatgg cggcccacaa
     3601 ctcatatctc gcgtaatcat ccaaacgcta ggcccgagta ctctactgtt ctcgagacac
     3661 ctaccataat atgtacccaa tcagacatga caacagtcaa ccaaacatac cctgatccgg
     3721 ccaaaccctg acatccctag atgatggtta ggcacacaaa tggaaaacgg gactcgttgg
     3781 ttaggtggga acgcttgtat tgcagccagc caaccgactc tcctcgtccc tctcgagtcc
     3841 cgttattgcc gtttgaccca gccgcccagc cgccaaacag ctgagggagc caatggtcta
     3901 atacaccgtc caacggcccc agtgatgttt caaacttgat tgacagcgaa cgaaacccct
     3961 gaaacccgtt atagatatgt atagatttga cggggtagct tggccctaat acgactcact
     4021 atagggcgaa ttggagctcc accgcggtgg cggccgctag aaggagcagt ccatctgcgt
     4081 gaatcacgag agaatcagct actttgaatc gatggatgca gctaccagaa gtcactcagt
     4141 tcgttcaaag ccacatcact gggcacttcc attgggacag gcattgatcg gacgagaccg
     4201 acttctggcc gctttcaaca gccacattat atccatgtca cggctacgcg cggccttcgg
     4261 taacagaaaa gcacacagac agcgattgtg acatggattc gggcaaagca ttggtggtcg
     4321 caccaggtca tcctgagtgt gcagtggctg ctattcagat ttcatctaac tgcgggagag
     4381 gggttcaaag gggcgtgacg tcacagacaa cgggtgaagg acgaagattg cctcacttct
     4441 ttgctagcaa tttgcctgca aagaaggcgc acatgtcaag caaacaactg ggaaaccact
     4501 attgaatacc cacaatgcaa agctcggaag gtacgtcttg attgcagtgt gtcgagtgtc
     4561 aaaaaagaag caagtgttca tgcaagccaa aattggcacc tcctccactt ctccgagtgc
     4621 cccacccgaa cctccaggcg agatggccgg aacataccat ccgcgttggg attatgacgt
     4681 atctccttct tcttcacatg attccatccc gttgttgctt gtttgcgagc tgtgacggga
     4741 gatcgtagat gccacttagg gccaggcagg cagtgcaggc agccaggaac acaagcttcc
     4801 aacttggtca tctcgattgc cgattaaggg aaccaaatgc ctatataaga ctgtcctccc
     4861 acctccccaa taccattctt ttcttcttcc atcatcagcc aacaaagcaa tcacatcttc
     4921 actacttcaa atcaacacaa cactcaaacc actttcacaa cccctcacat caaccaaatc
     4981 tagaactagt ggatccccgg gttaattaac atgtcctcaa gtactactcc agcagcgctc
     5041 aaagcagcca tccttatcgt ttcaacgaca gcagctcaag accccgcaac ggactcggcc
     5101 gacgtcactc tccgccaggt cttcgatgaa caacaagccc aatgggaggt ccatgaatcc
     5161 aaaatcgttc ccgacgatgt cgtgcaaatc cagaagcaaa tcatccactg gacaaaccaa
     5221 gccaccgccg caagtggcga caacaacaac aacaacaaca ccaacaccat caacctcatc
     5281 gtaaccacag gcggcaccgg cttcgccgtg tccgaccaaa ccaccgaagc cgtctcggct
     5341 ctcctacaca agaatgctcc tggcctggtc cacggcatgc tggcggcttc gctgcaagtg
     5401 accccctttg ccatgatgtc acgacctgtg gctggcgtca ggaacggcac cgttatcgtt
     5461 gcgctgcctg ggtcccccaa gggcgcgaag gagaatctgc aagcagtgat caaaatgctg
     5521 ccccacgctt gcatgcaggc tgctggtgct aactctagag aacttgaaga tttgccttcc
     5581 ccacctcccc ctctttcccc tcctcctact accagccccc ataaacagac agaagacaaa
     5641 ggagttcaat gtgaggaaga ggaagaagag aagaaagaca gtggtgttgc ttcaacagaa
     5701 gatagttcct catcacatat aactgcagca gccattgctg ccaagaagca tccattctac
     5761 accagtcctg ctgttgtcat ggcacacggt gaacagccca tccctggtct catcaattat
     5821 tcccatcatt caacagatga acggattcca gactccatca tttctcgtgg tgttcaggtg
     5881 ctcccacgag acacagcctc cctcagcact actccttcag aatcgcctcg tgctcaggct
     5941 acatctcgcc tctctacagc ttcctgccca acaccaaaag tccagtccag gtgcagcagc
     6001 aaggagaaca ttctcagagc cagtcacagt gctgtcgata tcaccaaggt ggctagaaga
     6061 atgctctctg tctccgaagc gctctcccaa atagcgcaac acacgccgat gccgcaaatc
     6121 gtcacctcca aagtcgatag ctcgctcatc ggcgccgttc tggcgcagga cgtttcggcg
     6181 cgcgaaaatg tgcccgcttt tcgcgctagc atcgtcgacg gctacgccgt agtcgtgccc
     6241 caagacggga acatgcgcgg cgtgttcccc gtcgtgtcgg tttcgcatgc cgctcctgga
     6301 gatggggaaa aaatgccgac gctcaaagag ggcgagattg cccggatcac cacgggtgca
     6361 cccctgcctc cgggtgcgac atcggttatt atggtggagg atacggtgtt ggtgtcgacg
     6421 acgtcatcca agacggagga tgcggaggat ggaaagggag tggaggagga gaaggaggtg
     6481 gagattttag cagctgataa agttcgcgaa ggcgaaaatg ttagggaggt gggaagtgat
     6541 atcaagcagg ggcaactgat tctaggcaag ggagatcagg tgtctgctgt ggggggtgag
     6601 attggtctgt tggccgccgt gggtgtgggt gaggtgcagg tgtaccgacg tcctgttgtt
     6661 ggcgttctat ctacgggtga tgagattgtg gaccatgatc gcccgggaga cttgcggctg
     6721 ggcgaggtgc gcgatacgaa ccggccaact ctcatttcgg cggcgagaga ttgggggtat
     6781 gaggtgattg atcttggagt ggcgcgggac aaggccggga ctttggagga gacgctgcgg
     6841 caggcattga ggcaggttga cgtggtgatc acgaccggcg gagtgtccat gggtgagctg
     6901 gatctcctca agccgacgat agagcgttcg ctgggcggaa caattcgctt tggtcgggtg
     6961 gcaatgaagc ccggcaagcc taccacgttt gctaccgttc cggtaaagga taacgacgga
     7021 gagagagttt ccaaggtaat cttctcgcta ccaggtaacc cggcatcggc tttggttacg
     7081 tttcacttgt ttgtactgcc gtctctgcac caaatgtcgg gcgtctcacc acctggcttg
     7141 ccaaaggtgc ctgtgacact gggccatgat ttctccctcg atagatctag acccgagtac
     7201 caccgggcca ttgtctccgt tagcagggac ggagtgctca ccgcggtcag cacaggagga
     7261 cagagaagct cgcgggtagg aagtatgaag ggggccaacg cgcttctctg catgccttgc
     7321 ggaccagagc cgttgcgcaa gggtgccaag gtggaagcgt tgcttatggg cggtttacgc
     7381 agcgatgtga tttaagaatt cgatatcaag cttatcgata ccgtcgacct cgaggggggg
     7441 cccggtacca gcttttgttc cctttagtga gggttaatag ggcgtgcacg gctatggggt
     7501 ctgaatgcta aaagacacca tttcccacac tccctctgat tttttttatg gttttttttt
     7561 atcttcattt ttcgccgtct tttattcggt tgtttgggag gttcacatgc aacgtgcaag
     7621 actgcaagta tattgagatg ggcgcttcat gcaatgcagc atcactgcag cgacattgtg
     7681 acatccacgt ttcgatcgtc tgtgcttccc cgttcatgta cgcatgatct gaaaaaaaaa
     7741 aaaaaaaaaa aaagttgatg taataatgaa aataaacacc cagggacgag caccccttct
     7801 ggaataggga gaaaaactaa agggacgttt gggtctctac acttaccaac agattcatgt
     7861 actgatcttc agcctgtaca gaacagtaca atgtccaaaa ctcgttatca ttactctatg
     7921 tgcccaaaat gacccagata tacatcaacc cactacatcc ttttgttctt gttttttttt
     7981 ttttttttgc tacttcgaaa agaatttccg tttagttgct cttgctcatg tgctcaagac
     8041 ggatgctgac cgcccttctg tgagcctcga gctcctcaac cttagccaac tgcatgacag
     8101 cctggccgac gtttttgaga ccctcggcag tcaagttgga gctggtaatg tgcttgacga
     8161 acgaggcgag attgacgcca gagtactgct tggcaaagcc ataggtagct atgtgtcttg
     8221 acgttagcag aagaatctgt gaagcggggc tatttatgat atgtaactta cgcagcgagt
     8281 ggttaacacc agcagagtaa tcgccaacgg actcaggagt ccaggcgcca atgaagacac
     8341 taccggcgtt catgacaaga tcgacagcct tctcggcctc cttgatctgg aggatcaagt
     8401 gctcaggagc gtacttgttg ctgagctcca tggcctcctc gacggtcttg acctgcacgg
     8461 tgatcgagtg ggcgatggag ccacggacaa tctggacgcg aggaagctcc gtagcctgac
     8521 ggtgaacctc gtcctcaata gcctgaagat gctcctcgtc gaggtcaata gcgatcagga
     8581 tgacctgact gtcaacgccg tgctcagcct gggacaggag atccgaggca acgaacgcgg
     8641 ggttggcgtc cttgtcagcg atgaccagca cctcggacgg gccagcgggc atgtcaatac
     8701 caacggcagc gttggtgtcg ttgctgacga acatcttggc agcagtgacg aactggttac
     8761 cggggccgag aatcttgtcg accttggtga tgctctcggt gccgtaggcc atggcagcta
     8821 cggcctgggc accgccggca agcacgatgg actcggcgcc aaccttgtga gcgacgtaga
     8881 caatctcggg agtgatggtt ccgtcggcgc ggggaggaga ggcgaacaca atcttgttgc
     8941 agccggcgac catggcggga acacccagca taagggcagt gctggggaga acggcggtac
     9001 cgccggggat gtagcagccg acggcctcga tgggacgaga gaaacggctg cagacaacac
     9061 cgggcatggt ctcgacctgg aggggcttct cctccttctg ggcggcgtgg aacttgcgga
     9121 tgttctcgaa ggacacgtcg atggcagcaa tggtctcctc agggagctgc ataagctcct
     9181 tggggaaggg cgccttcagg acggggctag taagagaggt agccttctcg aacttgtgag
     9241 tgtacgacag aacagccttg tcgccgttct tgcggacgtc ctcgatgatg gggacaatga
     9301 tcttgtagat ggcatcggac gacttttgcg caggacgctt gagagcagca tcgagctcct
     9361 cggtagagac cttggaggcg tcgaaacgtc tcatggtgat cttctcaggg gtggtctcct
     9421 gggcggcctc cttggtgaca ggggccgaag tggcagcgag agcggacgcc gcaggcttga
     9481 tgccctcctt ctcagcccac ttacccttag catctccagt cctgcgcttg accttccagc
     9541 tcttggcgtc aaggctcctt tcgatatcgg caagagtaac gccggcagca acggccctgg
     9601 taagagcaaa gtagaagaga tcggcagcct caaaggcgat ttcctggggg gtctgagcgg
     9661 tgcagagctc ctcagcctcc tccatgatct tggcccggac tagcttctca tcggagaaga
     9721 gacgggcagt gtaggagccc tcgggggcag actgtttcct cgaaatcaaa gtctgctcga
     9781 gcttgggaag gcctttgagc tgaccaaagc agccggactg atcgaggtgg cagaaacgac
     9841 ccttctgctt cacgacaaac ttgagagcat cgttatcgca gtcaagcgag atgcggacga
     9901 gctcctgagt gtctccggaa gtagcaccct tgtaccagag accgcgcttc cggctctgat
     9961 agacaccagt ctgtgtcctg agggcctcgt tcacactctc ggcactgctg tagaccagac
    10021 ccagagcagt gtcgtgctca tcaacgacaa cggtggggag aagaccatcg ggacggtcag
    10081 acttccagac gctcgagagg atggtggaga tggcaagctt cagctgctcg agttc
//
