LOCUS       sequence-460712-       16558 bp    DNA     circular SYN 15-APR-2025
DEFINITION  Wheat transformation vector used to express wheat Septoria tritici 
            blotch (Stb) resistance gene 15.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 16558)
  AUTHORS   Hafeez AN, Chartrain L, Feng C, Cambon F, Clarke M, Griffiths S, 
            Hayta S, Jiang M, Keller B, Kirby R, Kolodziej MC, Powell OR, 
            Smedley M, Steuernagel B, Xian W, Wingen LU, Cheng S, Saintenac C, 
            Wulff BBH, Brown JKM
  TITLE     Septoria tritici blotch resistance gene Stb15 encodes a lectin 
            receptor-like kinase
  JOURNAL   bioRxiv 2023.09.11.557217
REFERENCE   2  (bases 1 to 16558)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "bioRxiv 
            2023.09.11.557217"
COMMENT     Sequence Label: pGGG L2 TaStb15
FEATURES             Location/Qualifiers
     source          1..16558
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    20..44
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     rep_origin      complement(135..723)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(215..234)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(894..1709)
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     CDS             complement(894..1709)
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     1617..1636
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     primer_bind     1701..1720
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     rep_origin      2000..2435
                     /label=pSa ori
                     /note="origin of replication from bacterial plasmid pSa"
     misc_feature    2562..2584
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA 
                     (truncated)"
     misc_feature    2668..2692
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     polyA_signal    2781..2955
                     /label=CaMV poly(A) signal
                     /note="cauliflower mosaic virus polyadenylation signal"
     intron          3677..3866
                     /label=cat1 intron
                     /note="castor bean catalase intron, modified"
     protein_bind    6154..6175
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(6190..6195,6196..6213,6214..6220)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6228..6244
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     6233..6255
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     6252..6268
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     6252..6268
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     promoter        12356..14346
                     /label=Ubi promoter
                     /note="maize polyubiquitin gene promoter"
     terminator      16272..16524
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
ORIGIN
        1 ttgaagtgat ccgtttcctt gacaggatat attggcgggt aaactaagtc gctgtatgtg
       61 tttgtttgag atctcatgtg agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc
      121 gcgttgctgg cgtttttcca taggctccgc ccccctgacg agcatcacaa aaatcgacgc
      181 tcaagtcaga ggtggcgaaa cccgacagga ctataaagat accaggcgtt tccccctgga
      241 agctccctcg tgcgctctcc tgttccgacc ctgccgctta ccggatacct gtccgccttt
      301 ctcccttcgg gaagcgtggc gctttctcat agctcacgct gtaggtatct cagttcggtg
      361 taggtcgttc gctccaagct gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc
      421 gccttatccg gtaactatcg tcttgagtcc aacccggtaa gacacgactt atcgccactg
      481 gcagcagcca ctggtaacag gattagcaga gcgaggtatg taggcggtgc tacagagttc
      541 ttgaagtggt ggcctaacta cggctacact agaagaacag tatttggtat ctgcgctctg
      601 ctgaagccag ttaccttcgg aagaagagtt ggtagctctt gatccggcaa acaaaccacc
      661 gctggtagcg gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct
      721 caagaagatc ctttgatctt ttctacgggg tctgacgctc agtggaacga aaactcacgt
      781 taagggattt tggtcatgag attatcaaaa aggatcttca cctagatcct tttaaattaa
      841 aaatgaagtt ttaaatcaat ctaaagtata tatgtgtaac attggtctag tgattagaaa
      901 aactcatcga gcatcaaatg aaactgcaat ttattcatat caggattatc aataccatat
      961 ttttgaaaaa gccgtttctg taatgaagga gaaaactcac cgaggcagtt ccataggatg
     1021 gcaagatcct ggtatcggtc tgcgattccg actcgtccaa catcaataca acctattaat
     1081 ttcccctcgt caaaaataag gttatcaagt gagaaatcac catgagtgac gactgaatcc
     1141 ggtgagaatg gcaaaagttt atgcatttct ttccagactt gttcaacagg ccagccatta
     1201 cgctcgtcat caaaatcact cgcatcaacc aaaccgttat tcattcgtga ttgcgcctga
     1261 gcgagacgaa atacgcgatc gctgttaaaa ggacaattac aaacaggaat cgaatgcaac
     1321 cggcgcagga acactgccag cgcatcaaca atattttcac ctgaatcagg atattcttct
     1381 aatacctgga atgctgtttt ccctgggatc gcagtggtga gtaaccatgc atcatcagga
     1441 gtacggataa aatgcttgat ggtcggaaga ggcataaatt ccgtcagcca gtttagtctg
     1501 accatctcat ctgtaacatc attggcaacg ctacctttgc catgtttcag aaacaactct
     1561 ggcgcatcgg gcttcccata caatcggtag attgtcgcac ctgattgccc gacattatcg
     1621 cgagcccatt tatacccata taaatcagca tccatgttgg aatttaatcg cggccttgag
     1681 caagacgttt cccgttgaat atggctcata acaccccttg tattactgtt tatgtaagca
     1741 gacagtttta ttgttcatga tgatatattt ttatcttgtg caatgtaaca tcagagattt
     1801 tgagacacaa cgtggctttg ttgaataaat cgaacttttg ctgagttgaa ggatcagatc
     1861 acgcatcttc ccgacaacgc agaccgttcc gtggcaaagc aaaagttcaa aatccccaac
     1921 tggtccacct acaacaaagc tctcatcaac cgtggctccc tcactttctg gctggatgat
     1981 ggggcgattc aggcgatccc catccaacag cccgccgtcg agcgggcttt tttatccccg
     2041 gaagcctgtg gatagagggt agttatccac gtgaaaccgc taatgccccg caaagccttg
     2101 attcacgggg ctttccggcc cgctccaaaa actatccacg tgaaatcgct aatcagggta
     2161 cgtgaaatcg ctaatcggag tacgtgaaat cgctaataag gtcacgtgaa atcgctaatc
     2221 aaaaaggcac gtgagaacgc taatagccct ttcagatcaa cagcttgcaa acacccctcg
     2281 ctccggcaag tagttacagc aagtagtatg ttcaattagc ttttcaatta tgaatatata
     2341 tatcaattat tggtcgccct tggcttgtgg acaatgcgct acgcgcaccg gctccgcccg
     2401 tggacaaccg caagcggttg cccaccgtcg agcgcctttg cccacaaccc ggcggccggc
     2461 cgcaacagat cgttttataa attttttttt ttgaaaaaga aaaagcccga aaggcggcaa
     2521 cctctcgggc ttctggattt ccgatccccg gaattagatc ttggcaggat atattgtggt
     2581 gtaacgttta gtcatggttg atgggctgcc tgtatcgagt ggtgattttg tgccgagctg
     2641 ccggtcgggg agctgttggc tggctggtgg caggatatat tgtggtgtaa acaaattgac
     2701 gcttagacaa cttaataaca cattgcggac gtttttaatg tactggggtt gaacactcta
     2761 atgccgaatt cggatccagc gatctggatt ttagtactgg attttggttt taggaattag
     2821 aaattttatt gatagaagta ttttacaaat acaaatacat actaagggtt tcttatatgc
     2881 tcaacacatg agcgaaaccc tataggaacc ctaattccct tatctgggaa ctactcacac
     2941 attattatgg agaaactcga gcttgtcgat cgactctagc tagagaagcc tattcctttg
     3001 ccctcggacg agtgctgggg cgtcggtttc cactatcggc gagtacttct acacagccat
     3061 cggtccagac ggccgcgctt ctgcgggcga tttgtgtacg cccgacagtc ccggctccgg
     3121 atcggacgat tgcgtcgcat cgaccctgcg cccaagctgc atcatcgaaa ttgccgtcaa
     3181 ccaagctctg atagagttgg tcaagaccaa tgcggagcat atacgcccgg agccgcggcg
     3241 atcctgcaag ctccggatgc ctccgctcga agtagcgcgt ctgctgctcc atacaagcca
     3301 accacggcct ccagaagaag atgttggcga cctcgtattg ggaatccccg aacatcgcct
     3361 cgctccagtc aatgaccgct gttatgcggc cattgtccgt caggacattg ttggagccga
     3421 aatccgcgtg cacgaggtgc cggacttcgg ggcagtcctc ggcccaaagc atcagctcat
     3481 cgagagcctg cgcgacggac gcactgacgg tgtcgtccat cacagtttgc cagtgataca
     3541 catggggatc agcaatcgcg catatgaaat cacgccatgt agtgtattga ccgattcctt
     3601 gcggtccgaa tgggccgaac ccgctcgtct ggctaagatc ggccgcagcg atcgcatcca
     3661 tggcctccgc gaccggctgc agttatcatc atcatcatag acacacgaaa taaagtaatc
     3721 agattatcag ttaaagctat gtaatattta caccataacc aatcaattaa aaaatagatc
     3781 agtttaaaga aagatcaaag ctcaaaaaaa taaaaagaga aaagggtcct aaccaagaaa
     3841 atgaaggaga aaaactagaa atttacctgc agaacagcgg gcagttcggt ttcaggcagg
     3901 tcttgcaacg tgacaccctg tgcacggcgg gagatgcaat aggtcaggct ctcgctgaat
     3961 tccccaatgt caagcacttc cggaatcggg agcgcggccg atgcaaagtg ccgataaaca
     4021 taacgatctt tgtagaaacc atcggcgcag ctatttaccc gcaggacata tccacgccct
     4081 cctacatcga agctgaaagc acgagattct tcgccctccg agagctgcat caggtcggag
     4141 acgctgtcga acttttcgat cagaaacttc tcgacagacg tcgcggtgag ttcaggcttt
     4201 ttcattctgc aaaagagaac cagacaacag ggtaagtgcc tagcagtaaa caaacagaac
     4261 tcatcacaag caaacagcaa catcatattc ataccaacag gtcatgtgtg ttcatcacat
     4321 cattagtact aagcatgcca tcatccaagt atatcaaagt aagggcaaag agcatgcatg
     4381 atcatcaggt gcacaaaaga atcatcaaat tgtagcagta catatcttca tctatcatgc
     4441 atatctatcc ataacaggac gatgcatgtt gaccaggtaa aagctacagg atcctatagg
     4501 aacagcagcg tatatatctt caccaatctt agcatcttaa tcatgtggca catgcagttt
     4561 caatttaagc acatgagcta gttgattatg aggtaccaga gaatcatcaa actgatgtag
     4621 cagcatatat cctcatctat catgcaatct aatctaatct aactaaacag gaaaggtgtg
     4681 ctattcagtt aaaagctacc gcatcatata caaacggcag cataagaaaa agcataatca
     4741 tcttaatcat gcaacaaacg cagattcata ataagcccaa gagctagctt gtgatgatct
     4801 tattctactc tgatctacag caatcagata acgacctaac cttgcacatg gcaacaaaac
     4861 aatcgatcgg acgaatcagt tgtttgttcc tagctagcac catcgaacca gataatagat
     4921 gcacgtacag atcccgaaaa cgaacccaaa aacagggcag acctagctga acctaggcag
     4981 cgacccagca gatcgtgaga acgatctcat ctacgaacag cctagaagca accccacgat
     5041 tcccggacaa acgacctaaa atccccacaa atcacatgag catgacagga taaacagcgg
     5101 aaccgatcag atctacacga aaaccccacc tcccagccac ccacgatcag gaaacacgcg
     5161 gatctagcat gatttcgtca acgcctcagc ctagttccta gccacagacc aagcagaacc
     5221 accaaaccac gccgagcgag gagatggggc aagaggacgg gggacacgat cgccgtacct
     5281 tgaagcgggg gaaggatcgc cgagggtcgc gagaagagca atttggattt ggaacccggg
     5341 ggttgtgcgc tccgaacgat cagacgatgt gagattgtgg gaagaggcgc ggagggccct
     5401 gtatttatgg gctgcgacgg ggggaggaga ggtggggagg gttggggaag gaatccccca
     5461 cccgtgccgt gacggttccg ggccgtgtga gaggagcccg ctcgtgtccg ccacgcaatt
     5521 tccgcgatcg gagcggagct ttcgagaggc ggctggatgg ttggtggccg ttagatttgt
     5581 agacgccgtt aacgcctcgc ctccaccggg aagagttttg agcagccgct tatgacaatg
     5641 gcttaacgac gttagacgga gcgttagtgg caggccatga tagaatagac gtattgcaat
     5701 gggattataa ttaaataaat aagaatataa taagatatgg caagtcggca ctcatgacat
     5761 ggtgtccgaa atgatagtgc tcactttctt agccgagaaa gttgacgcga ctgatttaga
     5821 agttaagata ttatttctct cttcttttct ttcctcgtca tataaggatg aaataaactt
     5881 tagagattgc cggtagtgat tttggatttc ggcgatcagg cttggtttgc cggtttcgga
     5941 cggtgtgcct taggccaccc gcagtgtatc ttgtaatgtt caaccgataa gcaagggtgg
     6001 ggctcaagca agtagtaaac aactatgtca aatgtcacca tggttatggt cttgtttagt
     6061 tggcttcctc cgcaagaatt caagcttgga gtgagaccgc agctggcacg acaggtttcc
     6121 cgactggaaa gcgggcagtg agcgcaacgc aattaatgtg agttagctca ctcattaggc
     6181 accccaggct ttacacttta tgcttccggc tcgtatgttg tgtggaattg tgagcggata
     6241 acaatttcac acaggaaaca gctatgacca tgattacgcc aagcttgcat gcctgcaggg
     6301 gaccccatgg gcggtgggat gtcgcgagcg gattgacttg ccctctataa gggtgatggt
     6361 gtttagtttt agtttaagta catgtttaat tttaagttca agttcatgtt taggtttaaa
     6421 aactagacta tgtttcggcg gatgattatg tacattttgt gtcgaatttc gtttgtttaa
     6481 atcgaatcta tgttgaattt atgaatattt gctttttact ccgccaaatt taggagttcg
     6541 gccctaaatt tagtgaagta aaaatcctcc actaagttta ctcggccgtt tataaatcaa
     6601 gttcatcctt atcatgagtg atattatatt acgtgaggtc caaatcgacc acatgatcaa
     6661 aataagcttt gtcgtgaacc aatctgtggt tggatggtta ggacgagagt ggtatcccct
     6721 gttcattaga gttgaaattt catacttaat actgggttca tatttttcta gatttatttc
     6781 agggcttcca gccaatggcg gagcttcagt aaggttggat gggccatgac ctggccagcc
     6841 cagagcaaaa acagtggagg gttcaaatta gattaggcca gtttgagaaa aatagtgggc
     6901 ttttctttgt accggcccgc ccatatttgg catcgtagtt cttccagcaa tgtaccttta
     6961 gtgaaaggag tagtttccgc cgattacaaa gacgtgtgcg gtaacttcat caatgtcaat
     7021 gtcaatgtta agataatgtg ccggctcagt ctctcgcatg ttctcgtagg ggtaagatgt
     7081 gcctgcgctt ctgccagtca gttctccgaa aaaagaggat cacacaaaac gtcctttgcc
     7141 cacgtgaggg gggtgtaaac ctaacctgga cgtttgacat caagccgatc cggcgcttcg
     7201 tcccaaaatc tcgtcgcatg ggaacagttc aacttcaaca gagcatgcat catgtcctcc
     7261 tcctcagcga gacagatttt gcacttacta tcagtaaccg gaagggaggg aatatgagga
     7321 aggttgacga ttgtgggtgg caccctgtat tcccagactt tcaggggccc cgttccagcg
     7381 cgcacggggg ccaagcaggc ggcatgcaca gagggtgttc gacgaaatgc ctcaccgagc
     7441 gagcggcttt gttgtggggt tgcgttcaat tcaacctgac tacgtgcgcc gcttcaggct
     7501 ggtcatagtg gggagtaact tatactagtg tcatgcatat gacactagtc taagttacta
     7561 cctttatagt gcaaagtaac atattattag tgtcatatgt ggcttcattt aatggcttgt
     7621 agactcatct tatcttggga agcgctatat tacagtaaca tattatgtta ccacttctta
     7681 ttaactacat gccacataag caattttttt tttgaaatgt gctatgttac tacctaagtt
     7741 actcccacta tgactagcct cagtcggcgt cagggtagcg acagcaggtg aggtaatgcc
     7801 ggtcccattg ctttagttat gaatcgaaag atgtgcacct catttgattt tgactctctg
     7861 ccgtaagagt tcatgccagc atgcaccatt aatttgctaa atatcaaagt ataagacttt
     7921 ttattttgac aggatggagt actgacctgg tcagtcctgt ctacgcctgc ccaatagagc
     7981 cgttcaactg aggactctag atggcaaata ctcctactac tagccaagca tgtctgatgt
     8041 cgactgtgaa ccacatgtta cagtctctgt tttagcgtgc ttgtctgtgt accgaaatgc
     8101 tcaacatttt ccacactgaa tagtcatggc tatccattaa ttcaaccgca ctgccaacca
     8161 tatttgacct ggataattcc tgtatgatgt agcacaccat tgacttgcaa ccgaagctgt
     8221 tgcttggttc ccatatatct gttcagttag gatggacgga gcacatccat tagtcaccaa
     8281 gaaatacctc atcaaattca gtcaggacgg acggagcaca tccattgctc accaagaaat
     8341 acctcatcaa tggagagagc aagcaagcgg aggtctgtgc tcccgtcttc ctttgtttta
     8401 ctactcccct tcctcatcaa ccttgtgtgc cccggctgga aattcacgga tgcaggagac
     8461 acgctccttc ctggacaatc tctaaatgct agccaggtcc tactctcaaa caatggtgtc
     8521 ttcaagttgg gtttcaactt gccatatgat tatgattatt cccggtattg tcgttttggc
     8581 acctggttca ctaactcatc gccccatgat gatgattcac tggtttggca acctgatctg
     8641 gaagcgactt ttgattgcaa ttcatcatca gctaccctat cagagaatgg cacgctacat
     8701 ataaccgaca gctatacctc tgtctttttg accacttcta tctctgccat tgcagtgctt
     8761 cttgataacg gaaaccttgt attaagagac caagcaaaca gttcgcaggt gctttggcag
     8821 agttttgatt acccaactgg tacactgctc cctggaatgc gcctagggtc caattcaatt
     8881 atcggcaaga gcatgatcct tttttctgat cttagtaatt atactgagta tgacgtttat
     8941 attacaaatt ttactctgag agtggatgca acaagaaggc gaggttttat tattcagaag
     9001 aatccttttg gtacagtgtt tgctggtact tttcctagct ggatagttcg tgaagatgga
     9061 gactctgttc tgacactaaa tgacgcacat acatacatgc gtttaaatgg ctctggattt
     9121 gttgaatttg gtaagcaaga gacacgcagt tctattttgt ggtctgctcc agacagctta
     9181 tgcgactttg agtcaaactg tggaccttat gttatctgca aaaggccagg cttctgcata
     9241 tgccctcctg gttttgatcc attaagcaca acatcaagca gttctcttgg ctgttcgagg
     9301 gaggttccta tgaattgtga aagagacaat tcaactcatg gggaggtggc gtttaacccg
     9361 atagatggcg tctacaagtt ccccaagcat tcttggccct ctgatgccag aaatctgaaa
     9421 gagtgtgaag cctcttgctt aaaggactgc acctgtactg cctttgctta caatgtgaca
     9481 tgcctattat ggttttggga gttacggaac acagtggtgc ttgactctgg ttcaaatggc
     9541 aatcgtttgt acattcgtgt tggcaccaaa caacaacatg aaaatttggg tttcagagct
     9601 gctcctctgt ttctaacggc aatggccgcg ttagccatca ttgttattat cattggtctg
     9661 actgttttgt ggagatgcag aacaaagtta ttcagagcaa gaactgtgta tgaacacgga
     9721 agccttgtgg ttttctcatt tacacaaata aagaactcaa caaaaaaatt ctctgaaaaa
     9781 ctcggagaag gtggtttcgg ctgtgttttc aaggggacat tgccatgttc tgctgcagtg
     9841 gccgtcaaaa ggctgaaatg ccttggacaa ggagagaagc agtttcgagc agaggtgcag
     9901 accattggga tgattcaaca caaaaatctt gttcggctac ttggattctg tgctgatagc
     9961 agtagcaggt tattggtgta tgagtacatg gaaaaaggtt ctttaaactc acatctcttc
    10021 cgtaaggatt ttgcaaaact gagctggaag ctgcggtacc atatagcact tggaacagca
    10081 agaggcctgg cttatctgca cgaggagtgc aaggattgca ttatacactg cgacatgaag
    10141 ccggacaatg tacttcttga tgcacagttc tgtcctaaga ttgccgactt tggtatggcg
    10201 aagcttcttg gtcgggattt cagtagggcc ctgacaacaa tgagagggac catcggatat
    10261 cttgcaccgg agtggatctc agggctgccg atcacacata aggctgacgt ctacagctat
    10321 ggaatgatgc tttttgaaat catatcaggg cgaagaaatg cggagaaaat tgaacaaggg
    10381 aactttactt actttcccat ctttgctgca gtcaagctgc atgaagggga cgttatgtgc
    10441 ctgttggata gaaggttgga aggcgatgcg gatgtggagc agctgaggag agcttgcaga
    10501 attgcatgtt ggtgcattca agatgctgaa gatcataggc caatgatggg gcaagttgtt
    10561 cagatgctag aaggtgttct ggatgttgaa gtacctccca ttccaaggtc actgcagaat
    10621 tttgtgggca tggaggattg attgcacttg ctctacaggc atgtactgtc tctgaaatac
    10681 taaaagagct atcattatat gtcaagaatt aagtgttcaa agttatgtgt gttttatatt
    10741 ctctaaacta cgttatcccc ttatctgcta aggttctgaa ctgaatttgc tatcattata
    10801 gacttgtgat attgtgcctt cacttgtttc tctgttggca tgatggcttg tctgttcgac
    10861 aatcagaact gtaaatcttt atgtatttat acgaattgag ttcaagatat catgtgaatg
    10921 aattctagta gatgtactta ctgcaccaca tactgcggtc tttgcacaaa ctaattaact
    10981 aatcctgctg tttcaagcaa tggttcactg cagtagcaca cacgtttgca gaatatcaat
    11041 tatatctttt ttgtgctaaa tttacttgtt agcatggttg tagattcttg tgaataattg
    11101 ggtaaccaca ttttttttca aaaagggggg actccccggc ctctgcatca gaacgatgca
    11161 tagggccaca attgggtaac cacatgaaag tatggataat atgtaggcat cttggctgtc
    11221 tgtaatgcac aaaatttaga tagatccatg gacatttcag aatagaaatc taaaacaaac
    11281 atcaagtaat tttcaaaaaa gaaaatcctt ctcattgtta taattagcat aaaaagatgt
    11341 atcctctggt ttctgtgtag tgtcagcctc tgctttcagc ttggttggat ttttattttt
    11401 attttttgag gggggtgaaa acagttttgt tcaacaaata aggggccggg atacaaataa
    11461 tttcaagagt cccttagaac cacacatacc aaccaggatc taatgatgct gccgcctttg
    11521 ggagatcatg ttcttcaaaa ttggactccc tcatttcatt cttttctttg ccttgatgac
    11581 ttgaataatg gtgctgctga tgcccaagaa tggcttatcc aggtgattaa ccgttgccag
    11641 acagtccgct gtgatgatta tctttctcag attgagatca gaaaccaaat taagaccctc
    11701 taacctgttt tctgtggact ctgcctgatg gctttattta taaagtcggg tgtatgcttt
    11761 ttctctagaa gaacactcat tgcgctcatg atgccatgcc caagaatcat caacattccg
    11821 agggttgagt gggatattga gaacaacatg tgcgtccggt gcctgcagaa gtgcagatgt
    11881 ccccaaacta gatcagcgtt ctggtctttt tgacgcccca tccatgaatt cttcaactaa
    11941 cttcagaggg atggccgacc gtgggtgcag cgttcttagc atctctatta tccctttggc
    12001 agccaattgt cctcccagac agtggggtag ccaggcccca ggcaagaggg gccatggcct
    12061 aggcgtatgg ctgaaatcct tctctgactg tagcagtata actactatag cataggttgt
    12121 tagagcaact ccagtagata ttgtaaaagt tcatactgta aaaggttttt tgagtaaggg
    12181 gcgggttccg acagaacaga tactgtaaaa cctgtacgca aaaaatattt ctccttttat
    12241 ttctcataca aatttgcatg tcaactcgat atgatctact ctctctctgt tcctctttat
    12301 agtgtgcctt tgtgtgtagc gcttatacga aaacatgagc tggcgctctc gagctcggag
    12361 gtgcagcgtg acccggtcgt gcccctctct agagataatg agcattgcat gtctaagtta
    12421 taaaaaatta ccacatattt tttttgtcac acttgtttga agtgcagttt atctatcttt
    12481 atacatatat ttaaacttta ctctacgaat aatataatct atagtactac aataatatca
    12541 gtgttttaga gaatcatata aatgaacagt tagacatggt ctaaaggaca attgagtatt
    12601 ttgacaacag gactctacag ttttatcttt ttagtgtgca tgtgttctcc tttttttttg
    12661 caaatagctt cacctatata atacttcatc cattttatta gtacatccat ttagggttta
    12721 gggttaatgg tttttataga ctaatttttt tagtacatct attttattct attttagcct
    12781 ctaaattaag aaaactaaaa ctctatttta gtttttttat ttaataattt agatataaaa
    12841 tagaataaaa taaagtgact aaaaattaaa caaataccct ttaagaaatt aaaaaaacta
    12901 aggaaacatt tttcttgttt cgagtagata atgccagcct gttaaacgcc gtcgacgagt
    12961 ctaacggaca ccaaccagcg aaccagcagc gtcgcgtcgg gccaagcgaa gcagacggca
    13021 cggcatctct gtcgctgcct ctggacccct ctcgagagtt ccgctccacc gttggacttg
    13081 ctccgctgtc ggcatccaga aattgcgtgg cggagcggca gacgtgagcc ggcacggcag
    13141 gcggcctcct cctcctctca cggcacggca gctacggggg attcctttcc caccgctcct
    13201 tcgctttccc ttcctcgccc gccgtaataa atagacaccc cctccacacc ctctttcccc
    13261 aacctcgtgt tgttcggagc gcacacacac acaaccagat ctcccccaaa tccacccgtc
    13321 ggcacctccg cttcaaggta cgccgctcgt cctccccccc ccccctctct accttctcta
    13381 gatcggcgtt ccggtccatg gttagggccc ggtagttcta cttctgttca tgtttgtgtt
    13441 agatccgtgt ttgtgttaga tccgtgctgc tagcgttcgt acacggatgc gacctgtacg
    13501 tcagacacgt tctgattgct aacttgccag tgtttctctt tggggaatcc tgggatggct
    13561 ctagccgttc cgcagacggg atcgatttca tgattttttt tgtttcgttg catagggttt
    13621 ggtttgccct tttcctttat ttcaatatat gccgtgcact tgtttgtcgg gtcatctttt
    13681 catgcttttt tttgtcttgg ttgtgatgat gtggtctggt tgggcggtcg ttctagatcg
    13741 gagtagaatt ctgtttcaaa ctacctggtg gatttattaa ttttggatct gtatgtgtgt
    13801 gccatacata ttcatagtta cgaattgaag atgatggatg gaaatatcga tctaggatag
    13861 gtatacatgt tgatgcgggt tttactgatg catatacaga gatgcttttt gttcgcttgg
    13921 ttgtgatgat gtggtgtggt tgggcggtcg ttcattcgtt ctagatcgga gtagaatact
    13981 gtttcaaact acctggtgta tttattaatt ttggaactgt atgtgtgtgt catacatctt
    14041 catagttacg agtttaagat ggatggaaat atcgatctag gataggtata catgttgatg
    14101 tgggttttac tgatgcatat acatgatggc atatgcagca tctattcata tgctctaacc
    14161 ttgagtacct atctattata ataaacaagt atgttttata attattttga tcttgatata
    14221 cttggatgat ggcatatgca gcagctatat gtggattttt ttagccctgc cttcatacgc
    14281 tatttatttg cttggtactg tttcttttgt cgatgctcac cctgttgttt ggtgttactt
    14341 ctgcaggaat ggcgatgccg tatgcctctc tttccccggc aggcgaccgc cgctcctccc
    14401 cggccgccac cgccaccgcc tccctcctcc ccttctgccg ctcctccccc ttctccgccg
    14461 gcggcaatgg cggcatgggg gaggaggcgc ggatggacgg gaggtggatg gcgaggccgg
    14521 tgcccttcac ggcggcgcag tacgaggagc tggagcacca ggcgctcata tacaagtacc
    14581 tggtggccgg cgtgtccgtc ccgccggatc tcgtgctccc catccgccgc ggcatcgagt
    14641 ccctcgccgc ccgcttctac cacaaccccc tcgccatcgg gtacggatcg tacctgggca
    14701 agaaggtgga tccggagccg ggccggtgcc ggcgcacgga cggcaagaag tggcggtgcg
    14761 ccaaggaggc cgcctccgac tccaagtatt gcgagcgcca catgcaccgc ggccgcaacc
    14821 gttcaagaaa gcctgtggaa acgcagctcg tctcgcactc ccagccgccg gccgcctccg
    14881 tcgtgccgcc cctcgccacc ggcttccaca accactccct ctaccccgcc atcggcggca
    14941 ccaacggtgg tggaggcggg gggaacaacg gcatgtccat gcccggcacg ttctcctccg
    15001 cgctggggcc gcctcagcag cacatgggca acaatgccgc ctctccctac gcggctctcg
    15061 gcggcgccgg aacatgcaaa gatttcaggt ataccgcata tggaataaga tctttggcag
    15121 acgagcagag tcagctcatg acagaagcca tgaacacctc cgtggagaac ccatggcgcc
    15181 tgccgccatc ttctcaaacg actacattcc cgctctcaag ctactctcct cagcttggag
    15241 caacgagtga cctgggtcag aacaacagca gcaacaacaa cagcggcgtc aaggccgagg
    15301 gacagcagca gcagcagccg ctctccttcc cggggtgcgg cgacttcggc agcggcgact
    15361 ccgcgaagca ggagaaccag acgctgcggc cgttcttcga cgagtggccc aagacgaggg
    15421 actcgtggtc ggacctgacc gacgacaact cgaacgtcgc ctccttctcg gccacccagc
    15481 tgtcgatctc gatacccatg acgtcctccg acttctccgc cgccagctcc cagtcgccca
    15541 acggcatgct gttcgccggc gagatgtacg cggccgctgc catgcagcag caacacctga
    15601 tgcagatgaa ccagagcatg atggggggct acgcttcctc taccactgcc accactgatc
    15661 tcattcagca gtacctggat gagaacaagc agctgatcct ggccatcctc gacaaccaga
    15721 acaacggcaa ggtggaggag tgcgcacgga accaagctaa gctccagcag aacctcatgt
    15781 acctcgccgc catcgccgac agccagcctc cgcagacggc atcgctgtct cagtacccgt
    15841 ccaacctgat gatgcagtcc gggccgcggt acatgcagca gcagtcggcg cagatgatgt
    15901 cgccgcagtc gctgatggcg gcgcggtcgt cgatgatgta cgcgcagcag gccatgtcgc
    15961 cgctccagca gcagcagcag cagcagcagc accaggcggc cgcgcacggc cagctgggga
    16021 tgtcctccgg cgcgaccacc gggttcaacc tcctgcacgg tgaggccagc atgggcggcg
    16081 gcggcggcgc cactggcaac agcatgatga acgccagcgt cttttcggac tatggccgcg
    16141 gcggcagcgg cgccaaggag gggtcgacct cgctgtcggc cgacgctcgc ggcgccaact
    16201 ctggcgcgca cagcggcgac ggggagtacc tcaagggcac cgaggaggaa ggaagctagg
    16261 cttgtcaagc agatcgttca aacatttggc aataaagttt cttaagattg aatcctgttg
    16321 ccggtcttgc gatgattatc atataatttc tgttgaatta cgttaagcat gtaataatta
    16381 acatgtaatg catgacgtta tttatgagat gggtttttat gattagagtc ccgcaattat
    16441 acatttaata cgcgatagaa aacaaaatat agcgcgcaaa ctaggataaa ttatcgcgcg
    16501 cggtgtcatc tatgttacta gatcgacgct ttacgaggat gcacatgtga ccgaggga
//
