LOCUS       sequence-460940-       12482 bp    DNA     circular SYN 15-APR-2025
DEFINITION  Episomal plasmid expresses hTBX3.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12482)
  TITLE     Improved differentiation ability in porcine induced pluripotent stem
            cell by employing additional reprogramming factor TBX3
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 12482)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pCXLE-hTBX3
FEATURES             Location/Qualifiers
     source          1..12482
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    31..619
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(84..104)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(502..513)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(710..729)
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     polyA_signal    775..830
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(829..848)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     primer_bind     complement(1191..1207)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1191..1207)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(1204..1226)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    1215..1231
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(1239..1245,1246..1263,1264..1269))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    1284..1305
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     protein_bind    1394..1427
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     CDS             1888..3813
                     /codon_start=1
                     /product="Epstein-Barr nuclear antigen 1, also known as 
                     EBNA-1"
                     /label=EBNA1
                     /note="crucial for latent viral infection, and for episomal
                     amplification of vectors containing the oriP origin (Okita 
                     et al., 2013)"
                     /translation="MSDEGPGTGPGNGLGEKGDTSGPEGSGGSGPQRRGGDNHGRGRGR
                     GRGRGGGRPGAPGGSGSGPRHRDGVRRPQKRPSCIGCKGTHGGTGAGAGAGGAGAGGAG
                     AGGGAGAGGGAGGAGGAGGAGAGGGAGAGGGAGGAGGAGAGGGAGAGGGAGGAGAGGGA
                     GGAGGAGAGGGAGAGGGAGGAGAGGGAGGAGGAGAGGGAGAGGAGGAGGAGAGGAGAGG
                     GAGGAGGAGAGGAGAGGAGAGGAGAGGAGGAGAGGAGGAGAGGAGGAGAGGGAGGAGAG
                     GGAGGAGAGGAGGAGAGGAGGAGAGGAGGAGAGGGAGAGGAGAGGGGRGRGGSGGRGRG
                     GSGGRGRGGSGGRRGRGRERARGGSRERARGRGRGRGEKRPRSPSSQSSSSGSPPRRPP
                     PGRRPFFHPVGEADYFEYHQEGGPDGEPDVPPGAIEQGPADDPGEGPSTGPRGQGDGGR
                     RKKGGWFGKHRGQGGSNPKFENIAEGLRALLARSHVERTTDEGTWVAGVFVYGGSKTSL
                     YNLRRGTALAIPQCRLTPLSRLPFGMAPGPGPQPGPLRESIVCYFMVFLQTHIFAEVLK
                     DAIKDLVMTKPAPTCNIRVTVCSFDDGVDLPPWFPPMVEGAAAEGDDGDDGDEGGDGDE
                     GEEGQE"
     protein_bind    3981..4014
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     rep_origin      4201..5990
                     /label=oriP
                     /note="Epstein-Barr virus oriP replication origin (Yates et
                     al., 2000)"
     primer_bind     complement(6531..6548)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(6702..7290)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6782..6801)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(7461..8252,8253..8321))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8084..8103
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8322..8426)
                     /gene="bla"
                     /label=AmpR promoter
     enhancer        8457..8836
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        8838..9116
                     /label=chicken beta-actin promoter
     intron          9117..10133
                     /label=chimeric intron
                     /note="chimera between introns from chicken beta-actin and 
                     rabbit beta-globin"
     primer_bind     10057..10079
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
     primer_bind     10141..10160
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     protein_bind    10192..10216
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     regulatory      12204..12213
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     protein_bind    complement(12453..12477)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 aattcaagct tcggggacta gtcatatgat aatcaacctc tggattacaa aatttgtgaa
       61 agattgactg gtattcttaa ctatgttgct ccttttacgc tatgtggata cgctgcttta
      121 atgcctttgt atcatgctat tgcttcccgt atggctttca ttttctcctc cttgtataaa
      181 tcctggttgc tgtctcttta tgaggagttg tggcccgttg tcaggcaacg tggcgtggtg
      241 tgcactgtgt ttgctgacgc aacccccact ggttggggca ttgccaccac ctgtcagctc
      301 ctttccggga ctttcgcttt ccccctccct attgccacgg cggaactcat cgccgcctgc
      361 cttgcccgct gctggacagg ggctcggctg ttgggcactg acaattccgt ggtgttgtcg
      421 gggaagctga cgtcctttcc atggctgctc gcctgtgttg ccacctggat tctgcgcggg
      481 acgtccttct gctacgtccc ttcggccctc aatccagcgg accttccttc ccgcggcctg
      541 ctgccggctc tgcggcctct tccgcgtctt cgccttcgcc ctcagacgag tcggatctcc
      601 ctttgggccg cctccccgca tcggtacaat tcactcctca ggtgcaggct gcctatcaga
      661 aggtggtggc tggtgtggcc aatgccctgg ctcacaaata ccactgagat ctttttccct
      721 ctgccaaaaa ttatggggac atcatgaagc cccttgagca tctgacttct ggctaataaa
      781 ggaaatttat tttcattgca atagtgtgtt ggaatttttt gtgtctctca ctcggaagga
      841 catatgggag ggcaaatcat ttaaaacatc agaatgagta tttggtttag agtttggcaa
      901 catatgccca tatgctggct gccatgaaca aaggttggct ataaagaggt catcagtata
      961 tgaaacagcc ccctgctgtc cattccttat tccatagaaa agccttgact tgaggttaga
     1021 ttttttttat attttgtttt gtgttatttt tttctttaac atccctaaaa ttttccttac
     1081 atgttttact agccagattt ttcctcctct cctgactact cccagtcata gctgtccctc
     1141 ttctcttatg gagatccctc gacctgcagc ccaagcttgg cgtaatcatg gtcatagctg
     1201 tttcctgtgt gaaattgtta tccgctcaca attccacaca acatacgagc cggaagcata
     1261 aagtgtaaag cctggggtgc ctaatgagtg agctaactca cattaattgc gttgcgctca
     1321 ctgcccgctt tccagtcggg aaacctgtcg tgccagcgga tctcaattcc gatcatattc
     1381 aataaccctt aatataactt cgtataatgt atgctatacg aagttattag gtctgaagag
     1441 gagtttacgt ccagccaagc ttaggatcaa ttctcatgtt tgacagctta tcatcgataa
     1501 gctgatcctc acaggccgca cccagctttt cttccgttgc cccagtagca tctctgtctg
     1561 gtgaccttga agaggaagag gaggggtccc gagaatcccc atccctaccg tccagcaaaa
     1621 agggggacga ggaatttgag gcctggcttg aggctcagga cgcaaatctt gaggatgttc
     1681 agcgggagtt ttccgggctg cgagtaattg gtgatgagga cgaggatggt tcggaggatg
     1741 gggaattttc agacctggat ctgtctgaca gcgaccatga aggggatgag ggtggggggg
     1801 ctgttggagg gggcaggagt ctgcactccc tgtattcact gagcgtcgtc taataaagat
     1861 gtctattgat ctcttttagt gtgaatcatg tctgacgagg ggccaggtac aggacctgga
     1921 aatggcctag gagagaaggg agacacatct ggaccagaag gctccggcgg cagtggacct
     1981 caaagaagag ggggtgataa ccatggacga ggacggggaa gaggacgagg acgaggaggc
     2041 ggaagaccag gagccccggg cggctcagga tcagggccaa gacatagaga tggtgtccgg
     2101 agaccccaaa aacgtccaag ttgcattggc tgcaaaggga cccacggtgg aacaggagca
     2161 ggagcaggag cgggaggggc aggagcagga ggggcaggag caggaggagg ggcaggagca
     2221 ggaggagggg caggaggggc aggaggggca ggaggggcag gagcaggagg aggggcagga
     2281 gcaggaggag gggcaggagg ggcaggaggg gcaggagcag gaggaggggc aggagcagga
     2341 ggaggggcag gaggggcagg agcaggagga ggggcaggag gggcaggagg ggcaggagca
     2401 ggaggagggg caggagcagg aggaggggca ggaggggcag gagcaggagg aggggcagga
     2461 ggggcaggag gggcaggagc aggaggaggg gcaggagcag gaggggcagg aggggcagga
     2521 ggggcaggag caggaggggc aggagcagga ggaggggcag gaggggcagg aggggcagga
     2581 gcaggagggg caggagcagg aggggcagga gcaggagggg caggagcagg aggggcagga
     2641 ggggcaggag caggaggggc aggaggggca ggagcaggag gggcaggagg ggcaggagca
     2701 ggaggagggg caggaggggc aggagcagga ggaggggcag gaggggcagg agcaggaggg
     2761 gcaggagggg caggagcagg aggggcagga ggggcaggag caggaggggc aggaggggca
     2821 ggagcaggag gaggggcagg agcaggaggg gcaggagcag gaggtggagg ccggggtcga
     2881 ggaggcagtg gaggccgggg tcgaggaggt agtggaggcc ggggtcgagg aggtagtgga
     2941 ggccgccggg gtagaggacg tgaaagagcc agggggggaa gtcgtgaaag agccaggggg
     3001 agaggtcgtg gacgtggaga aaagaggccc aggagtccca gtagtcagtc atcatcatcc
     3061 gggtctccac cgcgcaggcc ccctccaggt agaaggccat ttttccaccc tgtaggggaa
     3121 gccgattatt ttgaatacca ccaagaaggt ggcccagatg gtgagcctga cgtgcccccg
     3181 ggagcgatag agcagggccc cgcagatgac ccaggagaag gcccaagcac tggaccccgg
     3241 ggtcagggtg atggaggcag gcgcaaaaaa ggagggtggt ttggaaagca tcgtggtcaa
     3301 ggaggttcca acccgaaatt tgagaacatt gcagaaggtt taagagctct cctggctagg
     3361 agtcacgtag aaaggactac cgacgaagga acttgggtcg ccggtgtgtt cgtatatgga
     3421 ggtagtaaga cctcccttta caacctaagg cgaggaactg cccttgctat tccacaatgt
     3481 cgtcttacac cattgagtcg tctccccttt ggaatggccc ctggacccgg cccacaacct
     3541 ggcccgctaa gggagtccat tgtctgttat ttcatggtct ttttacaaac tcatatattt
     3601 gctgaggttt tgaaggatgc gattaaggac cttgttatga caaagcccgc tcctacctgc
     3661 aatatcaggg tgactgtgtg cagctttgac gatggagtag atttgcctcc ctggtttcca
     3721 cctatggtgg aaggggctgc cgcggagggt gatgacggag atgacggaga tgaaggaggt
     3781 gatggagatg agggtgagga agggcaggag tgatgtaact tgttaggaga cgccctcaat
     3841 cgtattaaaa gccgtgtatt cccccgcact aaagaataaa tccccagtag acatcatgcg
     3901 tgctgttggt gtatttctgg ccatctgtct tgtcaccatt ttcgtcctcc caacatgggg
     3961 caattgccgg aacccttaat ataacttcgt ataatgtatg ctatacgaag ttattaggtc
     4021 cctcgaagag gttcactagc ggatctcaat tgggcatacc catgttgtca cgtcactcag
     4081 ctccgcgctc aacaccttct cgcgttggaa aacattagcg acatttacct ggtgagcaat
     4141 cagacatgcg acggctttag cctggcctcc ttaaattcac ctaagaatgg gagcaaccag
     4201 caggaaaagg acaagcagcg aaaattcacg cccccttggg aggtggcggc atatgcaaag
     4261 gatagcactc ccactctact actgggtatc atatgctgac tgtatatgca tgaggatagc
     4321 atatgctacc cggatacaga ttaggatagc atatactacc cagatataga ttaggatagc
     4381 atatgctacc cagatataga ttaggatagc ctatgctacc cagatataaa ttaggatagc
     4441 atatactacc cagatataga ttaggatagc atatgctacc cagatataga ttaggatagc
     4501 ctatgctacc cagatataga ttaggatagc atatgctacc cagatataga ttaggatagc
     4561 atatgctatc cagatatttg ggtagtatat gctacccaga tataaattag gatagcatat
     4621 actaccctaa tctctattag gatagcatat gctacccgga tacagattag gatagcatat
     4681 actacccaga tatagattag gatagcatat gctacccaga tatagattag gatagcctat
     4741 gctacccaga tataaattag gatagcatat actacccaga tatagattag gatagcatat
     4801 gctacccaga tatagattag gatagcctat gctacccaga tatagattag gatagcatat
     4861 gctatccaga tatttgggta gtatatgcta cccatggcaa cattagccca ccgtgctctc
     4921 agcgacctcg tgaatatgag gaccaacaac cctgtgcttg gcgctcaggc gcaagtgtgt
     4981 gtaatttgtc ctccagatcg cagcaatcgc gcccctatct tggcccgccc acctacttat
     5041 gcaggtattc cccggggtgc cattagtggt tttgtgggca agtggtttga ccgcagtggt
     5101 tagcggggtt acaatcagcc aagttattac acccttattt tacagtccaa aaccgcaggg
     5161 cggcgtgtgg gggctgacgc gtgcccccac tccacaattt caaaaaaaag agtggccact
     5221 tgtctttgtt tatgggcccc attggcgtgg agccccgttt aattttcggg ggtgttagag
     5281 acaaccagtg gagtccgctg ctgtcggcgt ccactctctt tccccttgtt acaaatagag
     5341 tgtaacaaca tggttcacct gtcttggtcc ctgcctggga cacatcttaa taaccccagt
     5401 atcatattgc actaggatta tgtgttgccc atagccataa attcgtgtga gatggacatc
     5461 cagtctttac ggcttgtccc caccccatgg atttctattg ttaaagatat tcagaatgtt
     5521 tcattcctac actagtattt attgcccaag gggtttgtga gggttatatt ggtgtcatag
     5581 cacaatgcca ccactgaacc ccccgtccaa attttattct gggggcgtca cctgaaacct
     5641 tgttttcgag cacctcacat acaccttact gttcacaact cagcagttat tctattagct
     5701 aaacgaagga gaatgaagaa gcaggcgaag attcaggaga gttcactgcc cgctccttga
     5761 tcttcagcca ctgcccttgt gactaaaatg gttcactacc ctcgtggaat cctgacccca
     5821 tgtaaataaa accgtgacag ctcatggggt gggagatatc gctgttcctt aggacccttt
     5881 tactaaccct aattcgatag catatgcttc ccgttgggta acatatgcta ttgaattagg
     5941 gttagtctgg atagtatata ctactacccg ggaagcatat gctacccgtt tagggttaac
     6001 aagggggcct tataaacact attgctaatg ccctcttgag ggtccgctta tcggtagcta
     6061 cacaggcccc tctgattgac gttggtgtag cctcccgtag tcttcctggg cccctgggag
     6121 gtacatgtcc cccagcattg gtgtaagagc ttcagccaag agttacacat aaaggcaatg
     6181 ttgtgttgca gtccacagac tgcaaagtct gctccaggat gaaagccact cagtgttggc
     6241 aaatgtgcac atccatttat aaggatgtca actacagtca gagaacccct ttgtgtttgg
     6301 tccccccccg tgtcacatgt ggaacagggc ccagttggca agttgtacca accaactgaa
     6361 gggattacat gcactgcccc gcgaagaagg ggcagagatg tcgtagtcag gtttagttcg
     6421 tccggggcgg ggatcgatcc tctagagtcg acgctagcgg atccgctgca ttaatgaatc
     6481 ggccaacgcg cggggagagg cggtttgcgt attgggcgct cttccgcttc ctcgctcact
     6541 gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat cagctcactc aaaggcggta
     6601 atacggttat ccacagaatc aggggataac gcaggaaaga acatgtgagc aaaaggccag
     6661 caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag gctccgcccc
     6721 cctgacgagc atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc gacaggacta
     6781 taaagatacc aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt tccgaccctg
     6841 ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc
     6901 tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac
     6961 gaaccccccg ttcagcccga ccgctgcgcc ttatccggta actatcgtct tgagtccaac
     7021 ccggtaagac acgacttatc gccactggca gcagccactg gtaacaggat tagcagagcg
     7081 aggtatgtag gcggtgctac agagttcttg aagtggtggc ctaactacgg ctacactaga
     7141 agaacagtat ttggtatctg cgctctgctg aagccagtta ccttcggaaa aagagttggt
     7201 agctcttgat ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag
     7261 cagattacgc gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc tacggggtct
     7321 gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg tcatgagatt atcaaaaagg
     7381 atcttcacct agatcctttt aaattaaaaa tgaagtttta aatcaatcta aagtatatat
     7441 gagtaaactt ggtctgacag ttaccaatgc ttaatcagtg aggcacctat ctcagcgatc
     7501 tgtctatttc gttcatccat agttgcctga ctccccgtcg tgtagataac tacgatacgg
     7561 gagggcttac catctggccc cagtgctgca atgataccgc gagacccacg ctcaccggct
     7621 ccagatttat cagcaataaa ccagccagcc ggaagggccg agcgcagaag tggtcctgca
     7681 actttatccg cctccatcca gtctattaat tgttgccggg aagctagagt aagtagttcg
     7741 ccagttaata gtttgcgcaa cgttgttgcc attgctacag gcatcgtggt gtcacgctcg
     7801 tcgtttggta tggcttcatt cagctccggt tcccaacgat caaggcgagt tacatgatcc
     7861 cccatgttgt gcaaaaaagc ggttagctcc ttcggtcctc cgatcgttgt cagaagtaag
     7921 ttggccgcag tgttatcact catggttatg gcagcactgc ataattctct tactgtcatg
     7981 ccatccgtaa gatgcttttc tgtgactggt gagtactcaa ccaagtcatt ctgagaatag
     8041 tgtatgcggc gaccgagttg ctcttgcccg gcgtcaatac gggataatac cgcgccacat
     8101 agcagaactt taaaagtgct catcattgga aaacgttctt cggggcgaaa actctcaagg
     8161 atcttaccgc tgttgagatc cagttcgatg taacccactc gtgcacccaa ctgatcttca
     8221 gcatctttta ctttcaccag cgtttctggg tgagcaaaaa caggaaggca aaatgccgca
     8281 aaaaagggaa taagggcgac acggaaatgt tgaatactca tactcttcct ttttcaatat
     8341 tattgaagca tttatcaggg ttattgtctc atgagcggat acatatttga atgtatttag
     8401 aaaaataaac aaataggggt tccgcgcaca tttccccgaa aagtgccacc tgggtcgaca
     8461 ttgattattg actagttatt aatagtaatc aattacgggg tcattagttc atagcccata
     8521 tatggagttc cgcgttacat aacttacggt aaatggcccg cctggctgac cgcccaacga
     8581 cccccgccca ttgacgtcaa taatgacgta tgttcccata gtaacgccaa tagggacttt
     8641 ccattgacgt caatgggtgg agtatttacg gtaaactgcc cacttggcag tacatcaagt
     8701 gtatcatatg ccaagtacgc cccctattga cgtcaatgac ggtaaatggc ccgcctggca
     8761 ttatgcccag tacatgacct tatgggactt tcctacttgg cagtacatct acgtattagt
     8821 catcgctatt accatggtcg aggtgagccc cacgttctgc ttcactctcc ccatctcccc
     8881 ccccctcccc acccccaatt ttgtatttat ttatttttta attattttgt gcagcgatgg
     8941 gggcgggggg gggggggggg cgcgcgccag gcggggcggg gcggggcgag gggcggggcg
     9001 gggcgaggcg gagaggtgcg gcggcagcca atcagagcgg cgcgctccga aagtttcctt
     9061 ttatggcgag gcggcggcgg cggcggccct ataaaaagcg aagcgcgcgg cgggcgggag
     9121 tcgctgcgcg ctgccttcgc cccgtgcccc gctccgccgc cgcctcgcgc cgcccgcccc
     9181 ggctctgact gaccgcgtta ctcccacagg tgagcgggcg ggacggccct tctcctccgg
     9241 gctgtaatta gcgcttggtt taatgacggc ttgtttcttt tctgtggctg cgtgaaagcc
     9301 ttgaggggct ccgggagggc cctttgtgcg gggggagcgg ctcggggggt gcgtgcgtgt
     9361 gtgtgtgcgt ggggagcgcc gcgtgcggct ccgcgctgcc cggcggctgt gagcgctgcg
     9421 ggcgcggcgc ggggctttgt gcgctccgca gtgtgcgcga ggggagcgcg gccgggggcg
     9481 gtgccccgcg gtgcgggggg ggctgcgagg ggaacaaagg ctgcgtgcgg ggtgtgtgcg
     9541 tgggggggtg agcagggggt gtgggcgcgt cggtcgggct gcaacccccc ctgcaccccc
     9601 ctccccgagt tgctgagcac ggcccggctt cgggtgcggg gctccgtacg gggcgtggcg
     9661 cggggctcgc cgtgccgggc ggggggtggc ggcaggtggg ggtgccgggc ggggcggggc
     9721 cgcctcgggc cggggagggc tcgggggagg ggcgcggcgg cccccggagc gccggcggct
     9781 gtcgaggcgc ggcgagccgc agccattgcc ttttatggta atcgtgcgag agggcgcagg
     9841 gacttccttt gtcccaaatc tgtgcggagc cgaaatctgg gaggcgccgc cgcaccccct
     9901 ctagcgggcg cggggcgaag cggtgcggcg ccggcaggaa ggaaatgggc ggggagggcc
     9961 ttcgtgcgtc gccgcgccgc cgtccccttc tccctctcca gcctcggggc tgtccgcggg
    10021 gggacggctg ccttcggggg ggacggggca gggcggggtt cggcttctgg cgtgtgaccg
    10081 gcggctctag agcctctgct aaccatgttc atgccttctt ctttttccta cagctcctgg
    10141 gcaacgtgct ggttattgtg ctgtctcatc attttggcaa agaattatca aacaagtttg
    10201 tacaaaaaag caggctacca tgagcctctc catgagagat ccggtcattc ctgggacaag
    10261 catggcctac catccgttcc tacctcaccg ggcgccggac ttcgccatga gcgcggtgct
    10321 gggtcaccag ccgccgttct tccccgcgct gacgctgcct cccaacggcg cggcggcgct
    10381 ctcgctgccg ggcgccctgg ccaagccgat catggatcaa ttggtggggg cggccgagac
    10441 cggcatcccg ttctcctccc tggggcccca ggcgcatctg aggcctttga agaccatgga
    10501 gcccgaagaa gaggtggagg acgaccccaa ggtgcacctg gaggctaaag aactttggga
    10561 tcagtttcac aagcggggca ccgagatggt cattaccaag tcgggaaggc gaatgtttcc
    10621 tccatttaaa gtgagatgtt ctgggctgga taaaaaagcc aaatacattt tattgatgga
    10681 cattatagct gctgatgact gtcgttataa atttcacaat tctcggtgga tggtggctgg
    10741 taaggccgac cccgaaatgc caaagaggat gtacattcac ccggacagcc ccgctactgg
    10801 ggaacagtgg atgtccaaag tcgtcacttt ccacaaactg aaactcacca acaacatttc
    10861 agacaaacat ggatttactt tggccttccc aagtgatcac gctacgtggc aggggaatta
    10921 tagttttggt actcagacta tattgaactc catgcacaaa taccagcccc ggttccacat
    10981 tgtaagagcc aatgacatct tgaaactccc ttatagtaca tttcggacat acttgttccc
    11041 cgaaactgaa ttcatcgctg tgactgcata ccagaatgat aagataaccc agttaaaaat
    11101 agacaacaac ccttttgcaa aaggtttccg ggacactgga aatggccgaa gagaaaaaag
    11161 aaaacagctc accctgcagt ccatgagggt gtttgatgaa agacacaaaa aggagaatgg
    11221 gacctctgat gagtcctcca gtgaacaagc agctttcaac tgcttcgccc aggcttcttc
    11281 tccagccgcc tccactgtag ggacatcgaa cctcaaagat ttatgtccca gcgagggtga
    11341 gagcgacgcc gaggccgaga gcaaagagga gcatggcccc gaggcctgcg acgcggccaa
    11401 gatctccacc accacgtcgg aggagccctg ccgtgacaag ggcagccccg cggtcaaggc
    11461 tcaccttttc gctgctgagc ggccccggga cagcgggcgg ctggacaaag cgtcgcccga
    11521 ctcacgccat agccccgcca ccatctcgtc cagcactcgc ggcctgggcg cggaggagcg
    11581 caggagcccg gttcgcgagg gcacagcgcc ggccaaggtg gaagaggcgc gcgcgctccc
    11641 gggcaaggag gccttcgcgc cgctcacggt gcagacggac gcggccgccg cgcacctggc
    11701 ccagggcccc ctgcctggcc tcggcttcgc cccgggcctg gcgggccaac agttcttcaa
    11761 cgggcacccg ctcttcctgc accccagcca gtttgccatg gggggcgcct tctccagcat
    11821 ggcggccgct ggcatgggtc ccctcctggc cacggtttct ggggcctcca ccggtgtctc
    11881 gggcctggat tccacggcca tggcctctgc cgctgcggcg cagggactgt ccggggcgtc
    11941 cgcggccacc ctgcccttcc acctccagca gcacgtcctg gcctctcagg gcctggccat
    12001 gtcccctttc ggaagcctgt tcccttaccc ctacacgtac atggccgcag cggcggccgc
    12061 ctcctctgcg gcagcctcca gctcggtgca ccgccacccc ttcctcaatc tgaacaccat
    12121 gcgcccgcgg ctgcgctaca gcccctactc catcccggtg ccggtcccgg acggcagcag
    12181 tctgctcacc accgccctgc cctccatggc ggcggccgcg gggcccctgg acggcaaagt
    12241 cgccgccctg gccgccagcc cggcctcggt ggcagtggac tcgggctctg aactcaacag
    12301 ccgctcctcc acgctctcct ccagctccat gtccttgtcg cccaaactct gcgcggagaa
    12361 agaggcggcc accagcgaac tgcagagcat ccagcggttg gttagcggct tggaagccaa
    12421 gccggacagg tcccgcagcg cgtccccgta ggacccagct ttcttgtaca aagtggttcg
    12481 at
//
