LOCUS       sequence-46102-e         876 bp    DNA     linear   UNA 14-APR-2025
DEFINITION  AAV expression of Ef1a-driven, cre-dependent, stabilized step 
            function opsin (SSFO) for optogenetic activation..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 876)
  AUTHORS   Yizhar O, Fenno LE, Prigge M, Schneider F, Davidson TJ, O'Shea DJ, 
            Sohal VS, Goshen I, Finkelstein J, Paz JT, Stehfest K, Fudim R, 
            Ramakrishnan C, Huguenard JR, Hegemann P, Deisseroth K
  TITLE     Neocortical excitation/inhibition balance in information processing 
            and social dysfunction.
  JOURNAL   Nature. 2011 Jul 27;477(7363):171-8. doi: 10.1038/nature10360.
  PUBMED    21796121
REFERENCE   2  (bases 1 to 876)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 14, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10"; journalName: 
            "Nature"; date: "2011-07-27- 27"; volume: "477"; issue: "7363"; 
            pages: "171-8"
COMMENT     Sequence Label: pAAV-Ef1a-DIO hChR2(C128S/D156A)-mCherry
FEATURES             Location/Qualifiers
     source          1..876
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    242..275
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     protein_bind    326..359
                     /label=lox2272
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (AAGTATCC) (Shaw et al., 2021). lox2272 sites are 
                     compatible with each other, but incompatible with loxP or 
                     loxN sites (Lee and Saito, 1988)."
     intron          399..602
                     /label=EF-1-alpha intron A
                     /note="truncated intron upstream of the start codon of 
                     human EF-1-alpha"
     primer_bind     complement(422..442)
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
ORIGIN
        1 ccctgaaggg cgagatcaag cagaggctga agctgaagga cggcggccac tacgacgctg
       61 aggtcaagac cacctacaag gccaagaagc ccgtgcagct gcccggcgcc tacaacgtca
      121 acatcaagtt ggacatcacc tcccacaacg aggactacac catcgtggaa cagtacgaac
      181 gcgccgaggg ccgccactcc accggcggca tggacgagct gtacaagtaa tagggcgcgc
      241 cataacttcg tataatgtat gctatacgaa gttattaaga ggtttcatat tgctaatagc
      301 agctacaatc cagctaccat tctgcataac ttcgtataaa gtatcctata cgaagttatt
      361 ccggagtcga ctctagagga tccggtacct cacgacacct gaaatggaag aaaaaaactt
      421 tgaaccactg tctgaggctt gagaatgaac caagatccaa actcaaaaag ggcaaattcc
      481 aaggagaatt acatcaagtg ccaagctggc ctaacttcag tctccaccca ctcagtgtgg
      541 ggaaactcca tcgcataaaa cccctccccc caacctaaag acgacgtact ccaaaagctc
      601 gagaactaat cgaggtgcct ggacggcgcc cggtactccg tggagtcaca tgaagcgacg
      661 gctgaggacg gaaaggccct tttcctttgt gtgggtgact cacccgcccg ctctcccgag
      721 cgccgcgtcc tccattttga gctccctgca gcagggccgg gaagcggcca tctttccgct
      781 cacgcaactg gtgccgaccg ggccagcctt gccgcccagg gcggggcgat acacggcggc
      841 gcgaggccag gcaccagagc aggccggcca gcttga
//
