LOCUS       sequence-461097-        9600 bp    DNA     circular SYN 15-APR-2025
DEFINITION  Express split-KRas fragments that are fused with CIDs and FPs.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9600)
  TITLE     Stains Lab split-GTPase Plasmids
REFERENCE   2  (bases 1 to 9600)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: 
            pIRES-mCerulean-GAI1-92-KRas/N12-KRas/13C(Q61L)-GID1-mVenus-CAAX
FEATURES             Location/Qualifiers
     source          1..9600
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        138..517
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        518..729
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     680..700
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     726..750
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     intron          890..1022
                     /label=chimeric intron
                     /note="chimera between introns from human beta-globin and 
                     immunoglobulin heavy chain genes"
     primer_bind     1005..1024
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     promoter        1067..1085
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             join(1091..1093,1094..1096,1097..1807)
                     /codon_start=1
                     /product="enhanced monomeric variant of CFP (Rizzo et al., 
                     2004)"
                     /label=mCerulean
                     /note=" mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTWGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNAISDNVYITADKQKNGIK
                     ANFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             1844..1861
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     misc_feature    join(2254..2828,2829..2831,2832..2840)
                     /label=IRES2
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(2421..2438)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     2648..2667
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             join(4499..4501,4502..5212)
                     /codon_start=1
                     /product="Venus YFP with monomerizing A206K mutation (Nagai
                     et al., 2002; Kremers et al., 2006)"
                     /label=mVenus
                     /note="mammalian codon-optimized"
                     /translation="VSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     LICTTGKLPVPWPTLVTTLGYGLQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYITADKQKNGIKA
                     NFKIRHNIEDGGVQLADHYQQNTPIGDGPVLLPDNHYLSYQSKLSKDPNEKRDHMVLLE
                     FVTAAGITLGMDELYK"
     polyA_signal    5304..5425
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5315..5334)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     5369..5388
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     rep_origin      5611..6066
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5698..5717)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     5908..5929
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        6183..6540
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     enhancer        6183..6374
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     complement(6206..6226)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      6391..6526
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             6591..7385
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(6645..6664)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     7255..7274
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    7449..7497
                     /note="synthetic polyadenylation signal"
     primer_bind     complement(7538..7557)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     7657..7679
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(7717..7735)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        7803..7907
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(7908..7976,7977..8768)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(8126..8145)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      8939..9527
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     9428..9447
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 tcaatattgg ccattagcca tattattcat tggttatata gcataaatca atattggcta
       61 ttggccattg catacgttgt atctatatca taatatgtac atttatattg gctcatgtcc
      121 aatatgaccg ccatgttggc attgattatt gactagttat taatagtaat caattacggg
      181 gtcattagtt catagcccat atatggagtt ccgcgttaca taacttacgg taaatggccc
      241 gcctggctga ccgcccaacg acccccgccc attgacgtca ataatgacgt atgttcccat
      301 agtaacgcca atagggactt tccattgacg tcaatgggtg gagtatttac ggtaaactgc
      361 ccacttggca gtacatcaag tgtatcatat gccaagtccg ccccctattg acgtcaatga
      421 cggtaaatgg cccgcctggc attatgccca gtacatgacc ttacgggact ttcctacttg
      481 gcagtacatc tacgtattag tcatcgctat taccatggtg atgcggtttt ggcagtacac
      541 caatgggcgt ggatagcggt ttgactcacg gggatttcca agtctccacc ccattgacgt
      601 caatgggagt ttgttttggc accaaaatca acgggacttt ccaaaatgtc gtaacaactg
      661 cgatcgcccg ccccgttgac gcaaatgggc ggtaggcgtg tacggtggga ggtctatata
      721 agcagagctc gtttagtgaa ccgtcagatc actagaagct ttattgcggt agtttatcac
      781 agttaaattg ctaacgcagt cagtgcttct gacacaacag tctcgaactt aagctgcagt
      841 gactctctta aggtagcctt gcagaagttg gtcgtgaggc actgggcagg taagtatcaa
      901 ggttacaaga caggtttaag gagaccaata gaaactgggc ttgtcgagac agagaagact
      961 cttgcgtttc tgataggcac ctattggtct tactgacatc cactttgcct ttctctccac
     1021 aggtgtccac tcccagttca attacagctc ttaaggctag agtacttaat acgactcact
     1081 ataggctagc atggtgagca agggcgagga gctgttcacc ggggtggtgc ccatcctggt
     1141 cgagctggac ggcgacgtaa acggccacaa gttcagcgtg tccggcgagg gcgagggcga
     1201 tgccacctac ggcaagctga ccctgaagtt catctgcacc accggcaagc tgcccgtgcc
     1261 ctggcccacc ctcgtgacca ccctgacctg gggcgtgcag tgcttcgccc gctaccccga
     1321 ccacatgaag cagcacgact tcttcaagtc cgccatgccc gaaggctacg tccaggagcg
     1381 caccatcttc ttcaaggacg acggcaacta caagacccgc gccgaggtga agttcgaggg
     1441 cgacaccctg gtgaaccgca tcgagctgaa gggcatcgac ttcaaggagg acggcaacat
     1501 cctggggcac aagctggagt acaacgccat cagcgacaac gtctatatca ccgccgacaa
     1561 gcagaagaac ggcatcaagg ccaacttcaa gatccgccac aacatcgagg acggcagcgt
     1621 gcagctcgcc gaccactacc agcagaacac ccccatcggc gacggccccg tgctgctgcc
     1681 cgacaaccac tacctgagca cccagtccaa gctgagcaaa gaccccaacg agaagcgcga
     1741 tcacatggtc ctgctggagt tcgtgaccgc cgccgggatc actctcggca tggacgagct
     1801 gtacaagggc ggtggctcat ctggcggagg tatgaagaga gatcatcatc atcatcatca
     1861 tcaagataag aagactatga tgatgaatga agaagacgac ggtaacggca tggatgagct
     1921 tctagctgtt cttggttaca aggttaggtc atccgaaatg gctgatgttg ctcagaaact
     1981 cgagcagctt gaagttatga tgtctaatgt tcaagaagac gatctttctc aactcgctac
     2041 tgagactgtt cactataatc cggcggagct ttacacgtgg cttgattcta tgctcaccga
     2101 ccttaatcag atctcgtacg cgtcccgggg cggtggctca tctggcggag gtgagctcat
     2161 gactgaatat aaacttgtgg tagttggagc tggttaagaa ttcacgcgtc gagcatgcat
     2221 ctagggcggc caattccgcc cctctccccc ccccccctct ccctcccccc cccctaacgt
     2281 tactggccga agccgcttgg aataaggccg gtgtgcgttt gtctatatgt tattttccac
     2341 catattgccg tcttttggca atgtgagggc ccggaaacct ggccctgtct tcttgacgag
     2401 cattcctagg ggtctttccc ctctcgccaa aggaatgcaa ggtctgttga atgtcgtgaa
     2461 ggaagcagtt cctctggaag cttcttgaag acaaacaacg tctgtagcga ccctttgcag
     2521 gcagcggaac cccccacctg gcgacaggtg cctctgcggc caaaagccac gtgtataaga
     2581 tacacctgca aaggcggcac aaccccagtg ccacgttgtg agttggatag ttgtggaaag
     2641 agtcaaatgg ctctcctcaa gcgtattcaa caaggggctg aaggatgccc agaaggtacc
     2701 ccattgtatg ggatctgatc tggggcctcg gtgcacatgc tttacatgtg tttagtcgag
     2761 gttaaaaaaa cgtctaggcc ccccgaacca cggggacgtg gttttccttt gaaaaacacg
     2821 atgataagct tgccacaacc cgggatcctc tagagtcgac atgggcgtag gcaagagtgc
     2881 cttgacgata cagctaattc agaatcattt tgtggacgaa tatgatccaa caatagagga
     2941 ttcctacagg aagcaagtag taattgatgg agaaacctgt ctcttggata ttctcgacac
     3001 agcaggtctg gaggagtaca gtgcaatgag ggaccagtac atgaggactg gggagggctt
     3061 tctttgtgta tttgccataa ataatactaa atcatttgaa gatattcacc attatagaga
     3121 acaaattaaa agagttaagg actctgaaga tgtacctatg gtcctagtag gaaataaatg
     3181 tgatttgcct tccagaacag tagacacaaa acaggctcag gacttagcaa gaagttatgg
     3241 aattcctttt attgaaacat cagcaaagac aagacagggt gttgatgatg ccttctatac
     3301 attagttcga gaaattcgaa aacataaaga aaagatgagc aaagatggtg gggatcccaa
     3361 ttgggagctc gtgtacacgg cgcgcctgca gggaggtggc tcatctggcg gaggtcagat
     3421 ctcgtacgcg tcccggggca tggctgcgag cgatgaagtt aatcttattg agagcagaac
     3481 agtggttcct ctcaatacat gggttttaat atccaacttc aaagtagcct acaatatcct
     3541 tcgtcgccct gatggaacct ttaaccgaca cttagctgag tatctagacc gtaaagtcac
     3601 tgcaaacgcc aatccggttg atggggtttt ctcgttcgat gtcttgattg atcgcaggat
     3661 caatcttcta agcagagtct atagaccagc ttatgcagat caagagcaac ctcctagtat
     3721 tttagatctc gagaagcctg ttgatggcga cattgtccct gttatattgt tcttccatgg
     3781 aggtagcttt gctcattctt ctgcaaacag tgccatctac gatactcttt gtcgcaggct
     3841 tgttggtttg tgcaagtgtg ttgttgtctc tgtgaattat cggcgtgcac cagagaatcc
     3901 atacccttgt gcttatgatg atggttggat tgctcttaat tgggttaact cgagatcttg
     3961 gcttaaatcc aagaaagact caaaggtcca tattttcttg gctggtgata gctctggagg
     4021 taacatcgcg cataatgtgg ctttaagagc gggtgaatcg ggaatcgatg ttttggggaa
     4081 cattctgctg aatcctatgt ttggtgggaa tgagagaacg gagtctgaga aaagtttgga
     4141 tgggaaatac tttgtgacgg ttagagaccg cgattggtac tggaaagcgt ttttacccga
     4201 gggagaagat agagagcatc cagcgtgtaa tccgtttagc ccgagaggga aaagcttaga
     4261 aggagtgagt ttccccaaga gtcttgtggt tgtcgcgggt ttggatttga ttagagattg
     4321 gcagttggca tacgcggaag ggctcaagaa agcgggtcaa gaggttaagc ttatgcattt
     4381 agagaaagca actgttgggt tttacctctt gcctaataac aatcatttcc ataatgttat
     4441 ggatgagatt tcggcgtttg taaacgcgga atgtggcggt ggctcatctg gcggaggtgt
     4501 gagcaagggc gaggagctgt tcaccggggt ggtgcccatc ctggtcgagc tggacggcga
     4561 cgtaaacggc cacaagttca gcgtgtccgg cgagggcgag ggcgatgcca cctacggcaa
     4621 gctgaccctg aagctcatct gcaccaccgg caagctgccc gtgccctggc ccaccctcgt
     4681 gaccaccctc ggctacggcc tgcagtgctt cgcccgctac cccgaccaca tgaagcagca
     4741 cgacttcttc aagtccgcca tgcccgaagg ctacgtccag gagcgcacca tcttcttcaa
     4801 ggacgacggc aactacaaga cccgcgccga ggtgaagttc gagggcgaca ccctggtgaa
     4861 ccgcatcgag ctgaagggca tcgacttcaa ggaggacggc aacatcctgg ggcacaagct
     4921 ggagtacaac tacaacagcc acaacgtcta tatcaccgcc gacaagcaga agaacggcat
     4981 caaggccaac ttcaagatcc gccacaacat cgaggacggc ggcgtgcagc tcgccgacca
     5041 ctaccagcag aacaccccca tcggcgacgg ccccgtgctg ctgcccgaca accactacct
     5101 gagctaccag tccaagctga gcaaagaccc caacgagaag cgcgatcaca tggtcctgct
     5161 ggagttcgtg accgccgccg ggatcactct cggcatggac gagctgtaca agaaaaaaaa
     5221 gaagaaaaag agcaagacca aatgcgtgat tatgtaagcg gccgcttccc tttagtgagg
     5281 gttaatgctt cgagcagaca tgataagata cattgatgag tttggacaaa ccacaactag
     5341 aatgcagtga aaaaaatgct ttatttgtga aatttgtgat gctattgctt tatttgtaac
     5401 cattataagc tgcaataaac aagttaacaa caacaattgc attcatttta tgtttcaggt
     5461 tcagggggag atgtgggagg ttttttaaag caagtaaaac ctctacaaat gtggtaaaat
     5521 ccgataagga tcgatccggg ctggcgtaat agcgaagagg cccgcaccga tcgcccttcc
     5581 caacagttgc gcagcctgaa tggcgaatgg acgcgccctg tagcggcgca ttaagcgcgg
     5641 cgggtgtggt ggttacgcgc agcgtgaccg ctacacttgc cagcgcccta gcgcccgctc
     5701 ctttcgcttt cttcccttcc tttctcgcca cgttcgccgg ctttccccgt caagctctaa
     5761 atcgggggct ccctttaggg ttccgattta gtgctttacg gcacctcgac cccaaaaaac
     5821 ttgattaggg tgatggttca cgtagtgggc catcgccctg atagacggtt tttcgccctt
     5881 tgacgttgga gtccacgttc tttaatagtg gactcttgtt ccaaactgga acaacactca
     5941 accctatctc ggtctattct tttgatttat aagggatttt gccgatttcg gcctattggt
     6001 taaaaaatga gctgatttaa caaaaattta acgcgaattt taacaaaata ttaacgctta
     6061 caatttcctg atgcggtatt ttctccttac gcatctgtgc ggtatttcac accgcatacg
     6121 cggatctgcg cagcaccatg gcctgaaata acctctgaaa gaggaacttg gttaggtacc
     6181 ttctgaggcg gaaagaacca gctgtggaat gtgtgtcagt tagggtgtgg aaagtcccca
     6241 ggctccccag caggcagaag tatgcaaagc atgcatctca attagtcagc aaccaggtgt
     6301 ggaaagtccc caggctcccc agcaggcaga agtatgcaaa gcatgcatct caattagtca
     6361 gcaaccatag tcccgcccct aactccgccc atcccgcccc taactccgcc cagttccgcc
     6421 cattctccgc cccatggctg actaattttt tttatttatg cagaggccga ggccgcctcg
     6481 gcctctgagc tattccagaa gtagtgagga ggcttttttg gaggcctagg cttttgcaaa
     6541 aagcttgatt cttctgacac aacagtctcg aacttaaggc tagagccacc atgattgaac
     6601 aagatggatt gcacgcaggt tctccggccg cttgggtgga gaggctattc ggctatgact
     6661 gggcacaaca gacaatcggc tgctctgatg ccgccgtgtt ccggctgtca gcgcaggggc
     6721 gcccggttct ttttgtcaag accgacctgt ccggtgccct gaatgaactg caggacgagg
     6781 cagcgcggct atcgtggctg gccacgacgg gcgttccttg cgcagctgtg ctcgacgttg
     6841 tcactgaagc gggaagggac tggctgctat tgggcgaagt gccggggcag gatctcctgt
     6901 catctcacct tgctcctgcc gagaaagtat ccatcatggc tgatgcaatg cggcggctgc
     6961 atacgcttga tccggctacc tgcccattcg accaccaagc gaaacatcgc atcgagcgag
     7021 cacgtactcg gatggaagcc ggtcttgtcg atcaggatga tctggacgaa gagcatcagg
     7081 ggctcgcgcc agccgaactg ttcgccaggc tcaaggcgcg catgcccgac ggcgaggatc
     7141 tcgtcgtgac ccatggcgat gcctgcttgc cgaatatcat ggtggaaaat ggccgctttt
     7201 ctggattcat cgactgtggc cggctgggtg tggcggaccg ctatcaggac atagcgttgg
     7261 ctacccgtga tattgctgaa gagcttggcg gcgaatgggc tgaccgcttc ctcgtgcttt
     7321 acggtatcgc cgctcccgat tcgcagcgca tcgccttcta tcgccttctt gacgagttct
     7381 tctgagcggg actctggggt tcgaaatgac cgaccaagcg acgcccaacc tgccatcacg
     7441 atggccgcaa taaaatatct ttattttcat tacatctgtg tgttggtttt ttgtgtgaat
     7501 cgatagcgat aaggatccgc gtatggtgca ctctcagtac aatctgctct gatgccgcat
     7561 agttaagcca gccccgacac ccgccaacac ccgctgacgc gccctgacgg gcttgtctgc
     7621 tcccggcatc cgcttacaga caagctgtga ccgtctccgg gagctgcatg tgtcagaggt
     7681 tttcaccgtc atcaccgaaa cgcgcgagac gaaagggcct cgtgatacgc ctatttttat
     7741 aggttaatgt catgataata atggtttctt agacgtcagg tggcactttt cggggaaatg
     7801 tgcgcggaac ccctatttgt ttatttttct aaatacattc aaatatgtat ccgctcatga
     7861 gacaataacc ctgataaatg cttcaataat attgaaaaag gaagagtatg agtattcaac
     7921 atttccgtgt cgcccttatt cccttttttg cggcattttg ccttcctgtt tttgctcacc
     7981 cagaaacgct ggtgaaagta aaagatgctg aagatcagtt gggtgcacga gtgggttaca
     8041 tcgaactgga tctcaacagc ggtaagatcc ttgagagttt tcgccccgaa gaacgttttc
     8101 caatgatgag cacttttaaa gttctgctat gtggcgcggt attatcccgt attgacgccg
     8161 ggcaagagca actcggtcgc cgcatacact attctcagaa tgacttggtt gagtactcac
     8221 cagtcacaga aaagcatctt acggatggca tgacagtaag agaattatgc agtgctgcca
     8281 taaccatgag tgataacact gcggccaact tacttctgac aacgatcgga ggaccgaagg
     8341 agctaaccgc ttttttgcac aacatggggg atcatgtaac tcgccttgat cgttgggaac
     8401 cggagctgaa tgaagccata ccaaacgacg agcgtgacac cacgatgcct gtagcaatgg
     8461 caacaacgtt gcgcaaacta ttaactggcg aactacttac tctagcttcc cggcaacaat
     8521 taatagactg gatggaggcg gataaagttg caggaccact tctgcgctcg gcccttccgg
     8581 ctggctggtt tattgctgat aaatctggag ccggtgagcg tgggtctcgc ggtatcattg
     8641 cagcactggg gccagatggt aagccctccc gtatcgtagt tatctacacg acggggagtc
     8701 aggcaactat ggatgaacga aatagacaga tcgctgagat aggtgcctca ctgattaagc
     8761 attggtaact gtcagaccaa gtttactcat atatacttta gattgattta aaacttcatt
     8821 tttaatttaa aaggatctag gtgaagatcc tttttgataa tctcatgacc aaaatccctt
     8881 aacgtgagtt ttcgttccac tgagcgtcag accccgtaga aaagatcaaa ggatcttctt
     8941 gagatccttt ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag
     9001 cggtggtttg tttgccggat caagagctac caactctttt tccgaaggta actggcttca
     9061 gcagagcgca gataccaaat actgttcttc tagtgtagcc gtagttaggc caccacttca
     9121 agaactctgt agcaccgcct acatacctcg ctctgctaat cctgttacca gtggctgctg
     9181 ccagtggcga taagtcgtgt cttaccgggt tggactcaag acgatagtta ccggataagg
     9241 cgcagcggtc gggctgaacg gggggttcgt gcacacagcc cagcttggag cgaacgacct
     9301 acaccgaact gagataccta cagcgtgagc tatgagaaag cgccacgctt cccgaaggga
     9361 gaaaggcgga caggtatccg gtaagcggca gggtcggaac aggagagcgc acgagggagc
     9421 ttccaggggg aaacgcctgg tatctttata gtcctgtcgg gtttcgccac ctctgacttg
     9481 agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac gccagcaacg
     9541 cggccttttt acggttcctg gccttttgct ggccttttgc tcacatggct cgacagatct
//
