LOCUS       sequence-461298-       11009 bp    DNA     circular SYN 14-APR-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11009)
  AUTHORS   Liu D, Vleugel M, Backer CB, Hori T, Fukagawa T, Cheeseman IM, 
            Lampson MA
  TITLE     Regulated targeting of protein phosphatase 1 to the outer 
            kinetochore by KNL1 opposes Aurora B kinase.
  JOURNAL   J Cell Biol. 2010 Mar 22;188(6):809-20. doi: 10.1083/jcb.201001006. 
            Epub 2010 Mar 15.
  PUBMED    20231380
REFERENCE   2  (bases 1 to 11009)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 14, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1083/jcb.201001006"; journalName: "J Cell Biol"; date: 
            "2010-03-22- 22"; volume: "188"; issue: "6"; pages: "809-20"
COMMENT     Sequence Label: mCherry KNL1 wt hardened and RVSF->AAAA
FEATURES             Location/Qualifiers
     source          1..11009
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        1..304
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        305..508
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     459..479
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     regulatory      547..556
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             553..1260
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(698..716)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     966..985
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     CDS             1306..1326
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     CDS             1339..1383
                     /codon_start=1
                     /product="affinity and epitope tag derived from pancreatic 
                     ribonuclease A"
                     /label=S-Tag
                     /translation="KETAAAKFERQHMDS"
     polyA_signal    7737..7858
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7774..7793)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     7828..7847
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      complement(7865..8320)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(8002..8023)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     8214..8233
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     promoter        8347..8451
                     /gene="bla"
                     /label=AmpR promoter
     promoter        8453..8810
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     enhancer        8453..8644
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     complement(8476..8496)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      8661..8796
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             8845..9639
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     CDS             8845..9639
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(8899..8918)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     9509..9528
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     complement(9827..9846)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    9871..9918
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     rep_origin      10247..10835
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     10736..10755
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt
       61 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca
      121 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc
      181 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta
      241 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac
      301 catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg
      361 atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg
      421 ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt
      481 acggtgggag gtctatataa gcagagctgg tttagtgaac cgtcagatcc gctagcgcta
      541 ccggtcgcca ccatggtgag caagggcgag gaggataaca tggccatcat caaggagttc
      601 atgcgcttca aggtgcacat ggagggctcc gtgaacggcc acgagttcga gatcgagggc
      661 gagggcgagg gccgccccta cgagggcacc cagaccgcca agctgaaggt gaccaagggt
      721 ggccccctgc ccttcgcctg ggacatcctg tcccctcagt tcatgtacgg ctccaaggcc
      781 tacgtgaagc accccgccga catccccgac tacttgaagc tgtccttccc cgagggcttc
      841 aagtgggagc gcgtgatgaa cttcgaggac ggcggcgtgg tgaccgtgac ccaggactcc
      901 tccctgcagg acggcgagtt catctacaag gtgaagctgc gcggcaccaa cttcccctcc
      961 gacggccccg taatgcagaa gaagaccatg ggctgggagg cctcctccga gcggatgtac
     1021 cccgaggacg gcgccctgaa gggcgagatc aagcagaggc tgaagctgaa ggacggcggc
     1081 cactacgacg ctgaggtcaa gaccacctac aaggccaaga agcccgtgca gctgcccggc
     1141 gcctacaacg tcaacatcaa gttggacatc acctcccaca acgaggacta caccatcgtg
     1201 gaacagtacg aacgcgccga gggccgccac tccaccggcg gcatggacga gctgtacaag
     1261 tccggagggg atcggtggag ctccaccggt ggcggccgct ctagagagaa tctttatttt
     1321 cagggcgccg ccaaattcaa agaaaccgct gctgctaaat tcgaacgcca gcacatggac
     1381 agcggaggtg gaggtactag tctcgagatg gatggggtgt cttcagaggc taatgaagaa
     1441 aatgacaata tagagagacc tgttagaaga cggcattctt caatattgaa acccccaagg
     1501 agtcctcttc aggacctcag aggtgggaat gaaacagttc aagagtcaaa cgcgttaagg
     1561 aataagaaaa actctcgtgc agccgccgct gcagatacta taaaggtatt ccagacggag
     1621 tctcatatga aaatagtgag aaagtcagaa atggaagaaa cagaagcagg agaaaatctt
     1681 cttttgatac agaataagaa attagaagat aattactgtg aaattactgg gatgaacaca
     1741 ttgctttctg ctcccattca tacccagatg caacagaagg agttttcaat tatagaacat
     1801 acccgtgaaa ggaaacatgc aaatgaccag acagtcattt tttcagatga aaaccagatg
     1861 gacctgacat caagtcacac tgtaatgatt accaaaggcc ttttagataa tcccataagt
     1921 ggaaagtcca ccaagataga taccacatca tttctagcta atttaaagct tcacaccgag
     1981 gactcaagaa tgaaaaaaga agtaaatttt tccgtggatc aaaacacttc ttcagaaaat
     2041 aaaatagatt tcaatgactt cataaaaaga ttgaaaacag gaaaatgtag tgcttttcct
     2101 gatgtgcctg ataaagaaaa ttttgagata cctatttatt ccaaggaacc gaacagtgcc
     2161 tcttctacac atcaaatgca tgtatctctt aaggaagatg aaaataacag taatattact
     2221 aggctcttta gagaaaaaga tgatgggatg aatttcaccc agtgtcatac agccaatatt
     2281 cagacattga ttcccacatc cagtgagacc aactcacggg aatctaaagg taatgatatt
     2341 acaatttatg gcaatgactt tatggacttg acatttaacc acactttgca gatcttacct
     2401 gcaacaggta atttttctga aatagaaaat caaactcaga atgccatgga tgtaacaaca
     2461 ggttatggaa ctaaagcttc aggaaataaa acagttttta agagtaaaca aaatactgct
     2521 tttcaagacc tttccataaa ctctgcagac aaaatacata ttaccagaag tcatattatg
     2581 ggggcagaaa ctcacatagt ctcacagact tgtaatcagg atgccagaat attagccatg
     2641 accccagaat ctatatattc taatccatct attcaaggtt gtaagactgt tttctattct
     2701 agttgtaatg atgccatgga aatgaccaaa tgtctctcaa atatgagaga ggagaaaaat
     2761 ttgctaaagc atgacagtaa ttattctaaa atgtattgca atccagatgc tatgtcttct
     2821 ctcacagaga aaactattta ttccggagag gagaacatgg acattaccaa gagtcataca
     2881 gttgcaatag ataatcaaat ttttaaacaa gatcaatcaa atgtgcaaat agcagctgca
     2941 ccaacacccg aaaaagaaat gatgctccaa aatcttatga ccacatcaga agatgagaaa
     3001 atgaatgtaa attgtaactc agttcctcat gtatctaagg aaagaataca gcagagcctg
     3061 tcaaatcctt tgtctatttc attgactgat agaaagactg aactcttatc aggtgaaaat
     3121 atggatttga ctgaaagtca cacaagtaac ttaggaagtc aggttcctct tgcagcttat
     3181 aatctagcac cggagagtac cagtgaatct cactctcaga gcaaaagctc ttcagatgaa
     3241 tgtgaagaaa ttaccaaaag tcgtaatgaa ccatttcagc gatcagacat aatagccaaa
     3301 aacagcttaa ccgacacctg gaacaaagac aaagattggg ttttgaagat tttgccctac
     3361 cttgataaag attctcctca gtcagctgat tgtaatcagg agatagcaac aagccataat
     3421 atagtctact gtggtggagt tcttgataaa caaataacta atagaaatac agtatcatgg
     3481 gaacaatctt tgttttctac cacaaagcca ttattttcat caggacagtt ctctatgaaa
     3541 aatcatgata ctgctataag tagtcataca gtgaaatctg tactaggcca gaattctaaa
     3601 ctggctgagc cactgaggaa aagtttaagc aatcccacac ctgactattg ccatgacaag
     3661 atgattatat gttcagagga agagcaaaat atggatctaa caaagagcca cactgtcgtc
     3721 attggatttg gtccttctga actacaagaa cttggtaaaa ctaatttaga acacactact
     3781 ggccagctaa caacaatgaa cagacagata gctgtaaaag ttgaaaaatg tggtaaaagt
     3841 cccatagaaa aaagtggagt gcttaaatct aactgtatta tggatgtgtt agaggacgaa
     3901 agtgtacaga aacctaaatt tccaaaggaa aagcaaaatg tcaaaatttg gggaaggaaa
     3961 agtgttggtg gaccaaaaat tgataagact attgtatttt cagaagacga taagaatgat
     4021 atggatatca ctaagagtta tacaatagaa ataaaccata gacctttatt agagaaacgt
     4081 gattgtcatt tggtgccatt ggcaggaact tctgaaacta ttttatatac atgtgggcag
     4141 gatgacatgg agatcactag gagtcacaca actgccttag aatgtaaaac tctcctgcca
     4201 aacgaaatag ctattaggcc catggacaaa accgtattgt tcacagataa ttacagtgat
     4261 ctggaagtca ccgattccca tactgttttc attgactgtc aagccacaga gaaaatactt
     4321 gaagaaaacc ctaaatttgg aataggaaaa ggaaaaaact tgggtgtttc ctttcctaag
     4381 gataatagct gtgttcaaga aatcgctgaa aaacaagcac tggctgtagg aaacaaaata
     4441 gttcttcaca ccgagcaaaa gcaacaactc tttgctgcta ctaatagaac tactaatgaa
     4501 atcatcaaat ttcatagtgc tgctatggat gaaaaggtca tagggaaagt tgtagaccag
     4561 gcctgtacat tggaaaaagc gcaagttgaa agctgtcagt taaataatag agatagaaga
     4621 aatgtggact ttacaagtag tcatgcaact gctgtttgtg gatccagtga taattattcc
     4681 tgtttaccaa atgttatttc ctgtactgat aatttggagg gtagtgccat gctcttatgt
     4741 gataaagatg aggaaaaagc caattattgc ccagtgcaaa atgatcttgc ttatgcaaat
     4801 gattttgcca gtgaatatta cttggaatct gagggacagc ctctctctgc tccttgtcct
     4861 ttgttagaga aggaagaagt tattcaaacc agtaccaaag gacagttaga ctgtgttata
     4921 acactgcaca aagatcaaga tctgattaag gatccacgaa atctattggc taatcaaact
     4981 ttagtatata gtcaagatct gggggagatg actaaactta attcaaagcg agtatctttt
     5041 aagcttccaa aggatcaaat gaaagtctat gttgatgaca tttatgttat tcctcagcct
     5101 catttctcaa ccgaccaacc tccattacct aaaaaaggac agagtagtat caataaagaa
     5161 gaagtaatac tgtctaaagc tggaaataag agtttaaata ttatagaaaa ttcctctgca
     5221 cccatatgtg aaaacaagcc caaaatactc aatagtgagg aatggtttgc tgcagcctgt
     5281 aaaaaagaac tgaaggaaaa tattcaaaca actaactata atacagctct agatctccac
     5341 agtaactcag acgtaactaa gcaagtcatt caaactcatg tcaatgctgg agaagcacca
     5401 gatcctgtaa ttacatctaa tgttccatgt tttcatagta tcaaaccaaa tctgaataat
     5461 ttgaatggaa aaactggaga gtttttagcc tttcaaactg ttcatctacc accccttcca
     5521 gagcaattac ttgaattagg aaataaggca cacaatgata tgcatatagt gcaagctaca
     5581 gaaatacata atattaacat aatctccagc aatgctaaag atagtagaga tgaggaaaat
     5641 aaaaagtctc ataatggagc tgaaaccacc tctctaccgc caaagacagt ttttaaagat
     5701 aaagtaagga gatgttcttt gggaatcttt ttgcctagat tgcccaacaa gagaaattgt
     5761 agtgtcactg gtattgatga cctggaacag attccagcag acacaactga tataaatcac
     5821 ttagaaactc agccggtctc tagcaaagat tcaggcattg gatctgttgc aggtaaactg
     5881 aacctaagtc cttctcaata tataaatgag gaaaatcttc ctgtatatcc tgatgagatc
     5941 aattcttcag actctattaa catagaaact gaggaaaagg ccttgattga gacataccaa
     6001 aaagagattt caccatatga aaataaaatg ggaaaaactt gcaatagcca aaaaagaacg
     6061 tgggtacaag aagaagaaga tattcataag gagaaaaaaa tcagaaaaaa tgagattaag
     6121 tttagtgata cgacacaaga tcgggagatt tttgatcacc atactgaaga ggatatagat
     6181 aaaagtgcta acagtgtatt gataaaaaac ctgagcagga ccccatctag ttgcagcagc
     6241 tctctggatt caatcaaggc tgatgggacc tctctggact tcagcactta ccgcagtagt
     6301 caaatggaat cacagtttct cagagatact atttgtgaag agagcttgag ggagaaactc
     6361 caagatggga gaataacaat aagggagttc tttatacttc tccaggtcca catcttgata
     6421 cagaaacccc gacagagcaa tctcccaggc aattttactg taaacacacc acctactcca
     6481 gaagacctga tgttaagtca atatgtttac cgacccaaga tacagattta tagagaagat
     6541 tgtgaggctc gtcgccaaaa gattgaagaa ttaaagcttt ctgcatcgaa ccaagataag
     6601 ctgttggttg atataaataa gaacctgtgg gaaaaaatga gacactgctc tgacaaagag
     6661 ctgaaggcct ttggaattta tcttaacaaa ataaagtcat gttttaccaa gatgactaaa
     6721 gtcttcactc accaaggaaa agtggctctg tatggcaagc tggtgcagtc agctcagaat
     6781 gagagggaga aacttcaaat aaagatagat gagatggata aaatacttaa gaagatcgat
     6841 aactgcctca ctgagatgga aacagaaact aagaatttgg aggatgaagg gaaaaacaat
     6901 cctgtggaag aatgggattc tgaaatgaga gctgcagaaa aagaattgga acagctgaaa
     6961 actgaagaag aggagcttca aagaaatctc ttagaactgg aggtacaaaa agagcagacc
     7021 cttgctcaaa tagactttat gcaaaaacaa agaaatagaa ctgaagagct actggatcag
     7081 ttgagcttgt ctgagtggga tgtcgttgag tggagtgatg atcaagctgt attcaccttt
     7141 gtttatgaca cgatacaact caccatcacc tttgaagagt cagttgttgg tttccctttc
     7201 ctggacaagc gttataggaa gattgttgat gtcaattttc aatctctgtt agatgaggat
     7261 caagctcctc cttcctccct tttagttcat aagcttattt tccagtacgt tgaagaaaag
     7321 gaatcctgga agaagacatg tacaacccag catcagttac ccaagatgct tgaagaattc
     7381 tcactggtag tgcaccattg cagactcctt ggagaggaga ttgagtattt aaagagatgg
     7441 ggaccaaatt ataacctaat gaacatagat attaataata atgaattgag acttttattc
     7501 tctagctccg cagcatttgc aaagtttgaa ataactttgt ttctctcagc ctattatcca
     7561 tctgtaccat taccttccac cattcagaat cacgttggga acactagcca agatgatatt
     7621 gctaccattc tatctaaagt gccactggag aacaactacc tgaagaatgt agtcaagcaa
     7681 atttaccaag atctgtttca ggactgccat ttctaccact agcaattgtt gttgttaact
     7741 tgtttattgc agcttataat ggttacaaat aaagcaatag catcacaaat ttcacaaata
     7801 aagcattttt ttcactgcat tctagttgtg gtttgtccaa actcatcaat gtatcttaac
     7861 gcgtaaattg taagcgttaa tattttgtta aaattcgcgt taaatttttg ttaaatcagc
     7921 tcatttttta accaataggc cgaaatcggc aaaatccctt ataaatcaaa agaatagacc
     7981 gagatagggt tgagtgttgt tccagtttgg aacaagagtc cactattaaa gaacgtggac
     8041 tccaacgtca aagggcgaaa aaccgtctat cagggcgatg gcccactacg tgaaccatca
     8101 ccctaatcaa gttttttggg gtcgaggtgc cgtaaagcac taaatcggaa ccctaaaggg
     8161 agcccccgat ttagagcttg acggggaaag ccggcgaacg tggcgagaaa ggaagggaag
     8221 aaagcgaaag gagcgggcgc tagggcgctg gcaagtgtag cggtcacgct gcgcgtaacc
     8281 accacacccg ccgcgcttaa tgcgccgcta cagggcgcgt caggtggcac ttttcgggga
     8341 aatgtgcgcg gaacccctat ttgtttattt ttctaaatac attcaaatat gtatccgctc
     8401 atgagacaat aaccctgata aatgcttcaa taatattgaa aaaggaagag tcctgaggcg
     8461 gaaagaacca gctgtggaat gtgtgtcagt tagggtgtgg aaagtcccca ggctccccag
     8521 caggcagaag tatgcaaagc atgcatctca attagtcagc aaccaggtgt ggaaagtccc
     8581 caggctcccc agcaggcaga agtatgcaaa gcatgcatct caattagtca gcaaccatag
     8641 tcccgcccct aactccgccc atcccgcccc taactccgcc cagttccgcc cattctccgc
     8701 cccatggctg actaattttt tttatttatg cagaggccga ggccgcctcg gcctctgagc
     8761 tattccagaa gtagtgagga ggcttttttg gaggcctagg cttttgcaaa gatcgatcaa
     8821 gagacaggat gaggatcgtt tcgcatgatt gaacaagatg gattgcacgc aggttctccg
     8881 gccgcttggg tggagaggct attcggctat gactgggcac aacagacaat cggctgctct
     8941 gatgccgccg tgttccggct gtcagcgcag gggcgcccgg ttctttttgt caagaccgac
     9001 ctgtccggtg ccctgaatga actgcaagac gaggcagcgc ggctatcgtg gctggccacg
     9061 acgggcgttc cttgcgcagc tgtgctcgac gttgtcactg aagcgggaag ggactggctg
     9121 ctattgggcg aagtgccggg gcaggatctc ctgtcatctc accttgctcc tgccgagaaa
     9181 gtatccatca tggctgatgc aatgcggcgg ctgcatacgc ttgatccggc tacctgccca
     9241 ttcgaccacc aagcgaaaca tcgcatcgag cgagcacgta ctcggatgga agccggtctt
     9301 gtcgatcagg atgatctgga cgaagagcat caggggctcg cgccagccga actgttcgcc
     9361 aggctcaagg cgagcatgcc cgacggcgag gatctcgtcg tgacccatgg cgatgcctgc
     9421 ttgccgaata tcatggtgga aaatggccgc ttttctggat tcatcgactg tggccggctg
     9481 ggtgtggcgg accgctatca ggacatagcg ttggctaccc gtgatattgc tgaagagctt
     9541 ggcggcgaat gggctgaccg cttcctcgtg ctttacggta tcgccgctcc cgattcgcag
     9601 cgcatcgcct tctatcgcct tcttgacgag ttcttctgag cgggactctg gggttcgaaa
     9661 tgaccgacca agcgacgccc aacctgccat cacgagattt cgattccacc gccgccttct
     9721 atgaaaggtt gggcttcgga atcgttttcc gggacgccgg ctggatgatc ctccagcgcg
     9781 gggatctcat gctggagttc ttcgcccacc ctagggggag gctaactgaa acacggaagg
     9841 agacaatacc ggaaggaacc cgcgctatga cggcaataaa aagacagaat aaaacgcacg
     9901 gtgttgggtc gtttgttcat aaacgcgggg ttcggtccca gggctggcac tctgtcgata
     9961 ccccaccgag accccattgg ggccaatacg cccgcgtttc ttccttttcc ccaccccacc
    10021 ccccaagttc gggtgaaggc ccagggctcg cagccaacgt cggggcggca ggccctgcca
    10081 tagcctcagg ttactcatat atactttaga ttgatttaaa acttcatttt taatttaaaa
    10141 ggatctaggt gaagatcctt tttgataatc tcatgaccaa aatcccttaa cgtgagtttt
    10201 cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga gatccttttt
    10261 ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt
    10321 tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc agagcgcaga
    10381 taccaaatac tgttcttcta gtgtagccgt agttaggcca ccacttcaag aactctgtag
    10441 caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc agtggcgata
    10501 agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg cagcggtcgg
    10561 gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac accgaactga
    10621 gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca
    10681 ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt ccagggggaa
    10741 acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt
    10801 tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg gcctttttac
    10861 ggttcctggc cttttgctgg ccttttgctc acatgttctt tcctgcgtta tcccctgatt
    10921 ctgtggataa ccgtattacc gccatgcatt agttattaat agtaatcaat tacggggtca
    10981 ttagttcata gcccatatat ggagttccg
//
