LOCUS       sequence-461420-         736 bp    DNA     linear   UNA 15-APR-2025
DEFINITION  Bacterial expression of N-terminally 6His tagged hnRNPA1 LCD 
            (186-320) D262V with all aromatic amino acids mutated to W keeping 
            the hexapeptide fibril core (SYNDFG) intact..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 736)
  AUTHORS   Das T, Zaidi F, Farag M, Ruff KM, Messing J, Taylor JP, Pappu RV, 
            Mittag T
  TITLE     Metastable condensates suppress conversion to amyloid fibrils.
  JOURNAL   bioRxiv [Preprint]. 2024 Mar 3:2024.02.28.582569. doi: 
            10.1101/2024.02.28.582569.
  PUBMED    38464104
REFERENCE   2  (bases 1 to 736)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "bioRxiv 
            [Preprint]. 2024 Mar 3:2024.02.28.582569. doi: 
            10.1101/2024.02.28.582569."
COMMENT     Sequence Label: A1-LCD allW D262V
FEATURES             Location/Qualifiers
     source          1..736
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     10..29
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     primer_bind     113..132
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        113..131
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     RBS             163..185
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             205..222
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     protein_bind    232..256
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             259..279
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     protein_bind    complement(692..716)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 ttccccatcg gtgatgtcgg cgatataggc gccagcaacc gcacctgtgg cgccggtgat
       61 gccggccacg atgcgtccgg cgtagaggat cgagatctcg atcccgcgaa attaatacga
      121 ctcactatag ggagaccaca acggtttccc tctagaaata attttgttta actttaagaa
      181 ggagatatac atatgtcgta ctaccatcac catcaccatc acctcgaatc aacaagtttg
      241 tacaaaaaag caggcttcga gaacctgtat tttcagggta gcatggcaag cgcaagcagc
      301 agccagcgtg gtcgtagcgg tagcggtaat tggggtggtg gtcgcggtgg tggttggggt
      361 ggcaatgata actggggtcg tggtggtaat tggagcggtc gcggaggctg gggtggttca
      421 agaggtggtg gcggatgggg tggtagtggt gatggttgga atggttgggg caatgatggt
      481 agcaactggg gtggcggtgg cagctataat gtctttggca attggaataa tcagagcagc
      541 aattggggtc cgatgaaagg tggtaactgg ggaggtcgta gcagcggtcc gtggggaggt
      601 ggcggtcagt ggtgggcaaa accgcgtaat caaggcggtt ggggaggtag ctctagcagc
      661 agcagctggg gttcaggtcg tcgttggtaa gacccagctt tcttgtacaa agtggttgat
      721 tcgaggctgc taacaa
//
