LOCUS       sequence-46146-e       13391 bp    DNA     circular SYN 15-APR-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 13391)
  AUTHORS   Bakota L, Brandt R, Heinisch JJ
  TITLE     Triple mammalian/yeast/bacterial shuttle vectors for single and 
            combined Lentivirus- and Sindbis virus-mediated infections of 
            neurons.
  JOURNAL   Mol Genet Genomics. 2012 Apr;287(4):313-24. Epub 2012 Feb 12.
  PUBMED    22327349
REFERENCE   2  (bases 1 to 13391)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Genet 
            Genomics."; date: "2012-04"; volume: "287(4)"; pages: "313-24. Epub 
            2012 Feb 12"
COMMENT     Sequence Label: pJJH1307
FEATURES             Location/Qualifiers
     source          1..13391
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     regulatory      1308..1317
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(1314..1316,1317..1319,1320..2033)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(1314..1316,1317..1319,1320..2030)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(1359..1380)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(1620..1639)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     1967..1988
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     misc_feature    2074..2662
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(2127..2147)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(2545..2556)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(2665..2681)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2666..2682)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     LTR             3187..3367
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(3393..3410)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    3399..3623
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      3669..4097
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(3756..3775)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     3966..3987
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(4106..4126)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        4111..4440
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      4291..4426
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     4353..4372
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        4488..4535
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             4554..4928
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    5058..5179
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5095..5114)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     5149..5168
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(5228..5244)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5228..5244)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(5241..5263)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    5252..5268
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(5276..5282,5283..5300,5301..5306))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    5321..5342
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(5459..5476)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(5630..6218)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(5710..5729)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(6389..7180,7181..7249))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     7012..7031
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(7250..7354)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     7422..7440
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(7478..7500)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     7600..7619
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     rep_origin      7636..8800
                     /label=2u ori
                     /note="yeast 2u plasmid origin of replication"
     protein_bind    9100..9133
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     promoter        complement(10490..10833)
                     /label=TEF promoter
                     /note="Ashbya gossypii TEF promoter"
     protein_bind    10886..10919
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     enhancer        11024..11403
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        11405..11603
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     11558..11578
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     LTR             11621..11801
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    11848..11973
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    12466..12699
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             12884..12928
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    13226..13343
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        join(13388..13391,1..1286)
                     /label=CaMKII promoter
                     /note="promoter for the mouse calcium/calmodulin-dependent 
                     kinase II alpha subunit"
ORIGIN
        1 atggccttag gtcacttcat ctccatgggg ttcttcttct gattttctag aaaatgagat
       61 gggggtgcag agagcttcct cagtgacctg cccagggtca catcagaaat gtcagagcta
      121 gaacttgaac tcagattact aatcttaaat tccatgcctt gggggcatgc aagtacgata
      181 tacagaagga gtgaactcat tagggcagat gaccaatgag tttaggaaag aagagtccag
      241 ggcagggtac atctacacca cccgcccagc cctgggtgag tccagccacg ttcacctcat
      301 tatagttgcc tctctccagt cctaccttga cgggaagcac aagcagaaac tgggacagga
      361 gccccaggag accaaatctt catggtccct ctgggaggat gggtggggag agctgtggca
      421 gaggcctcag gaggggccct gctgctcagt ggtgacagat aggggtgaga aagcagacag
      481 agtcattccg tcagcattct gggtctgttt ggtacttctt ctcacgctaa ggtggcggtg
      541 tgatatgcac aatggctaaa aagcagggag agctggaaag aaacaaggac agagacagag
      601 gccaagtcaa ccagaccaat tcccagagga agcaaagaaa ccattacaga gactacaagg
      661 gggaagggaa ggagagatga attagcttcc cctgtaaacc ttagaaccca gctgttgcca
      721 gggcaacggg gcaatacctg tctcttcaga ggagatgaag ttgccagggt aactacatcc
      781 tgtctttctc aaggaccatc ccagaatgtg gcacccacta gccgttacca tagcaactgc
      841 ctctttgccc cacttaatcc catcccgtct gttaaaaggg ccctatagtt ggaggtgggg
      901 gaggtaggaa gagcgatgat cacttgtgga ctaagtttgt tcgcatcccc ttctccaacc
      961 ccctcagtac atcaccctgg gagaacaagg tccacttgct tctgggccca cacagtcctg
     1021 cagtattgtg tatataaggc cagggcaaag gaggagcagg ttttaaagtg aaaggcaggc
     1081 aggtgttggg gaggcagtta ccggggcaac gggaacaggg cgtttcggag gtggttgcca
     1141 tggggacctg gatgctgacg aaggctcgcg aggctgtgag cagccacagt gccctgctca
     1201 gaagccccaa gctcgtcagt caagccggtt ctccgtttgc actcaggagc acgggcaggc
     1261 gagtggcccc tagttctggg ggcagcgggg atccccgggt accggtcgcc accatggtga
     1321 gcaagggcga ggagctgttc accggggtgg tgcccatcct ggtcgagctg gacggcgacg
     1381 taaacggcca caagttcagc gtgtccggcg agggcgaggg cgatgccacc tacggcaagc
     1441 tgaccctgaa gttcatctgc accaccggca agctgcccgt gccctggccc accctcgtga
     1501 ccaccctgac ctacggcgtg cagtgcttca gccgctaccc cgaccacatg aagcagcacg
     1561 acttcttcaa gtccgccatg cccgaaggct acgtccagga gcgcaccatc ttcttcaagg
     1621 acgacggcaa ctacaagacc cgcgccgagg tgaagttcga gggcgacacc ctggtgaacc
     1681 gcatcgagct gaagggcatc gacttcaagg aggacggcaa catcctgggg cacaagctgg
     1741 agtacaacta caacagccac aacgtctata tcatggccga caagcagaag aacggcatca
     1801 aggtgaactt caagatccgc cacaacatcg aggacggcag cgtgcagctc gccgaccact
     1861 accagcagaa cacccccatc ggcgacggcc ccgtgctgct gcccgacaac cactacctga
     1921 gcacccagtc cgccctgagc aaagacccca acgagaagcg cgatcacatg gtcctgctgg
     1981 agttcgtgac cgccgccggg atcactctcg gcatggacga gctgtacaag taaagcggcc
     2041 gcgactctag aattcgatat caagcttatc gataatcaac ctctggatta caaaatttgt
     2101 gaaagattga ctggtattct taactatgtt gctcctttta cgctatgtgg atacgctgct
     2161 ttaatgcctt tgtatcatgc tattgcttcc cgtatggctt tcattttctc ctccttgtat
     2221 aaatcctggt tgctgtctct ttatgaggag ttgtggcccg ttgtcaggca acgtggcgtg
     2281 gtgtgcactg tgtttgctga cgcaaccccc actggttggg gcattgccac cacctgtcag
     2341 ctcctttccg ggactttcgc tttccccctc cctattgcca cggcggaact catcgccgcc
     2401 tgccttgccc gctgctggac aggggctcgg ctgttgggca ctgacaattc cgtggtgttg
     2461 tcggggaaat catcgtcctt tccttggctg ctcgcctgtg ttgccacctg gattctgcgc
     2521 gggacgtcct tctgctacgt cccttcggcc ctcaatccag cggaccttcc ttcccgcggc
     2581 ctgctgccgg ctctgcggcc tcttccgcgt cttcgccttc gccctcagac gagtcggatc
     2641 tccctttggg ccgcctcccc gcatcgatac cgtcgacctc gagacctaga aaaacatgga
     2701 gcaatcacaa gtagcaatac agcagctacc aatgctgatt gtgcctggct agaagcacaa
     2761 gaggaggagg aggtgggttt tccagtcaca cctcaggtac ctttaagacc aatgacttac
     2821 aaggcagctg tagatcttag ccacttttta aaagaaaagg ggggactgga agggctaatt
     2881 cactcccaac gaagacaaga tatccttgat ctgtggatct accacacaca aggctacttc
     2941 cctgattggc agaactacac accagggcca gggatcagat atccactgac ctttggatgg
     3001 tgctacaagc tagtaccagt tgagcaagag aaggtagaag aagccaatga aggagagaac
     3061 acccgcttgt tacaccctgt gagcctgcat gggatggatg acccggagag agaagtatta
     3121 gagtggaggt ttgacagccg cctagcattt catcacatgg cccgagagct gcatccggac
     3181 tgtactgggt ctctctggtt agaccagatc tgagcctggg agctctctgg ctaactaggg
     3241 aacccactgc ttaagcctca ataaagcttg ccttgagtgc ttcaagtagt gtgtgcccgt
     3301 ctgttgtgtg actctggtaa ctagagatcc ctcagaccct tttagtcagt gtggaaaatc
     3361 tctagcaggg cccgtttaaa cccgctgatc agcctcgact gtgccttcta gttgccagcc
     3421 atctgttgtt tgcccctccc ccgtgccttc cttgaccctg gaaggtgcca ctcccactgt
     3481 cctttcctaa taaaatgagg aaattgcatc gcattgtctg agtaggtgtc attctattct
     3541 ggggggtggg gtggggcagg acagcaaggg ggaggattgg gaagacaata gcaggcatgc
     3601 tggggatgcg gtgggctcta tggcttctga ggcggaaaga accagctggg gctctagggg
     3661 gtatccccac gcgccctgta gcggcgcatt aagcgcggcg ggtgtggtgg ttacgcgcag
     3721 cgtgaccgct acacttgcca gcgccctagc gcccgctcct ttcgctttct tcccttcctt
     3781 tctcgccacg ttcgccggct ttccccgtca agctctaaat cgggggctcc ctttagggtt
     3841 ccgatttagt gctttacggc acctcgaccc caaaaaactt gattagggtg atggttcacg
     3901 tagtgggcca tcgccctgat agacggtttt tcgccctttg acgttggagt ccacgttctt
     3961 taatagtgga ctcttgttcc aaactggaac aacactcaac cctatctcgg tctattcttt
     4021 tgatttataa gggattttgc cgatttcggc ctattggtta aaaaatgagc tgatttaaca
     4081 aaaatttaac gcgaattaat tctgtggaat gtgtgtcagt tagggtgtgg aaagtcccca
     4141 ggctccccag caggcagaag tatgcaaagc atgcatctca attagtcagc aaccaggtgt
     4201 ggaaagtccc caggctcccc agcaggcaga agtatgcaaa gcatgcatct caattagtca
     4261 gcaaccatag tcccgcccct aactccgccc atcccgcccc taactccgcc cagttccgcc
     4321 cattctccgc cccatggctg actaattttt tttatttatg cagaggccga ggccgcctct
     4381 gcctctgagc tattccagaa gtagtgagga ggcttttttg gaggcctagg cttttgcaaa
     4441 aagctcccgg gagcttgtat atccattttc ggatctgatc agcacgtgtt gacaattaat
     4501 catcggcata gtatatcggc atagtataat acgacaaggt gaggaactaa accatggcca
     4561 agttgaccag tgccgttccg gtgctcaccg cgcgcgacgt cgccggagcg gtcgagttct
     4621 ggaccgaccg gctcgggttc tcccgggact tcgtggagga cgacttcgcc ggtgtggtcc
     4681 gggacgacgt gaccctgttc atcagcgcgg tccaggacca ggtggtgccg gacaacaccc
     4741 tggcctgggt gtgggtgcgc ggcctggacg agctgtacgc cgagtggtcg gaggtcgtgt
     4801 ccacgaactt ccgggacgcc tccgggccgg ccatgaccga gatcggcgag cagccgtggg
     4861 ggcgggagtt cgccctgcgc gacccggccg gcaactgcgt gcacttcgtg gccgaggagc
     4921 aggactgaca cgtgctacga gatttcgatt ccaccgccgc cttctatgaa aggttgggct
     4981 tcggaatcgt tttccgggac gccggctgga tgatcctcca gcgcggggat ctcatgctgg
     5041 agttcttcgc ccaccccaac ttgtttattg cagcttataa tggttacaaa taaagcaata
     5101 gcatcacaaa tttcacaaat aaagcatttt tttcactgca ttctagttgt ggtttgtcca
     5161 aactcatcaa tgtatcttat catgtctgta taccgtcgac ctctagctag agcttggcgt
     5221 aatcatggtc atagctgttt cctgtgtgaa attgttatcc gctcacaatt ccacacaaca
     5281 tacgagccgg aagcataaag tgtaaagcct ggggtgccta atgagtgagc taactcacat
     5341 taattgcgtt gcgctcactg cccgctttcc agtcgggaaa cctgtcgtgc cagctgcatt
     5401 aatgaatcgg ccaacgcgcg gggagaggcg gtttgcgtat tgggcgctct tccgcttcct
     5461 cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg agcggtatca gctcactcaa
     5521 aggcggtaat acggttatcc acagaatcag gggataacgc aggaaagaac atgtgagcaa
     5581 aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc
     5641 tccgcccccc tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga
     5701 caggactata aagataccag gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc
     5761 cgaccctgcc gcttaccgga tacctgtccg cctttctccc ttcgggaagc gtggcgcttt
     5821 ctcatagctc acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc aagctgggct
     5881 gtgtgcacga accccccgtt cagcccgacc gctgcgcctt atccggtaac tatcgtcttg
     5941 agtccaaccc ggtaagacac gacttatcgc cactggcagc agccactggt aacaggatta
     6001 gcagagcgag gtatgtaggc ggtgctacag agttcttgaa gtggtggcct aactacggct
     6061 acactagaag aacagtattt ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa
     6121 gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt
     6181 gcaagcagca gattacgcgc agaaaaaaag gatctcaaga agatcctttg atcttttcta
     6241 cggggtctga cgctcagtgg aacgaaaact cacgttaagg gattttggtc atgagattat
     6301 caaaaaggat cttcacctag atccttttaa attaaaaatg aagttttaaa tcaatctaaa
     6361 gtatatatga gtaaacttgg tctgacagtt accaatgctt aatcagtgag gcacctatct
     6421 cagcgatctg tctatttcgt tcatccatag ttgcctgact ccccgtcgtg tagataacta
     6481 cgatacggga gggcttacca tctggcccca gtgctgcaat gataccgcga gacccacgct
     6541 caccggctcc agatttatca gcaataaacc agccagccgg aagggccgag cgcagaagtg
     6601 gtcctgcaac tttatccgcc tccatccagt ctattaattg ttgccgggaa gctagagtaa
     6661 gtagttcgcc agttaatagt ttgcgcaacg ttgttgccat tgctacaggc atcgtggtgt
     6721 cacgctcgtc gtttggtatg gcttcattca gctccggttc ccaacgatca aggcgagtta
     6781 catgatcccc catgttgtgc aaaaaagcgg ttagctcctt cggtcctccg atcgttgtca
     6841 gaagtaagtt ggccgcagtg ttatcactca tggttatggc agcactgcat aattctctta
     6901 ctgtcatgcc atccgtaaga tgcttttctg tgactggtga gtactcaacc aagtcattct
     6961 gagaatagtg tatgcggcga ccgagttgct cttgcccggc gtcaatacgg gataataccg
     7021 cgccacatag cagaacttta aaagtgctca tcattggaaa acgttcttcg gggcgaaaac
     7081 tctcaaggat cttaccgctg ttgagatcca gttcgatgta acccactcgt gcacccaact
     7141 gatcttcagc atcttttact ttcaccagcg tttctgggtg agcaaaaaca ggaaggcaaa
     7201 atgccgcaaa aaagggaata agggcgacac ggaaatgttg aatactcata ctcttccttt
     7261 ttcaatatta ttgaagcatt tatcagggtt attgtctcat gagcggatac atatttgaat
     7321 gtatttagaa aaataaacaa ataggggttc cgcgcacatt tccccgaaaa gtgccacctg
     7381 acgtctaaga aaccattatt atcatgacat taacctataa aaataggcgt atcacgaggc
     7441 cctttcgtct cgcgcgtttc ggtgatgacg gtgaaaacct ctgacacatg cagctcccgg
     7501 agacggtcac agcttgtctg taagcggatg ccgggagcag acaagcccgt cagggcgcgt
     7561 cagcgggtgt tggcgggtgt cggggctggc ttaactatgc ggcatcagag cagattgtac
     7621 tgagagtgca ccataacgca tttaagcata aacacgcact atgccgttct tctcatgtat
     7681 atatatatac aggcaacacg cagatatagg tgcgacgtga acagtgagct gtatgtgcgc
     7741 agctcgcgtt gcattttcgg aagcgctcgt tttcggaaac gctttgaagt tcctattccg
     7801 aagttcctat tctctagcta gaaagtatag gaacttcaga gcgcttttga aaaccaaaag
     7861 cgctctgaag acgcactttc aaaaaaccaa aaacgcaccg gactgtaacg agctactaaa
     7921 atattgcgaa taccgcttcc acaaacattg ctcaaaagta tctctttgct atatatctct
     7981 gtgctatatc cctatataac ctacccatcc acctttcgct ccttgaactt gcatctaaac
     8041 tcgacctcta cattttttat gtttatctct agtattactc tttagacaaa aaaattgtag
     8101 taagaactat tcatagagtg aatcgaaaac aatacgaaaa tgtaaacatt tcctatacgt
     8161 agtatataga gacaaaatag aagaaaccgt tcataatttt ctgaccaatg aagaatcatc
     8221 aacgctatca ctttctgttc acaaagtatg cgcaatccac atcggtatag aatataatcg
     8281 gggatgcctt tatcttgaaa aaatgcaccc gcagcttcgc tagtaatcag taaacgcggg
     8341 aagtggagtc aggctttttt tatggaagag aaaatagaca ccaaagtagc cttcttctaa
     8401 ccttaacgga cctacagtgc aaaaagttat caagagactg cattatagag cgcacaaagg
     8461 agaaaaaaag taatctaaga tgctttgtta gaaaaatagc gctctcggga tgcatttttg
     8521 tagaacaaaa aagaagtata gattctttgt tggtaaaata gcgctctcgc gttgcatttc
     8581 tgttctgtaa aaatgcagct cagattcctt gtttgaaaaa ttagcgctct cgcgttgcat
     8641 ttttgtttta caaaaatgaa gcacagattc ttcgttggta aaatagcgct ttcgcgttgc
     8701 atttctgttc tgtaaaaatg cagctcagat tctttgtttg aaaaattagc gctctcgcgt
     8761 tgcatttttg ttctacaaaa tgaagcacag atgcttcgtt aacaaagata tgctattgaa
     8821 gtgcaagatg gaaacgcaga aaatgaaccg gggatgcgac gtgcaagatt acctatgcaa
     8881 tagatgcaat agtttctcca ggaaccgaaa tacatacatt gtcttccgta aagcgctaga
     8941 ctatatatta ttatacaggt tcaaatatac tatctgtttc agggaaaact cccaggttcg
     9001 gatgttcaaa attcaatgat gggtaacaag tgcttttcaa ttcatcattt tttttttatt
     9061 ctttttttct tcgtacgctg caggtcgaca acccttaata taacttcgta taatgtatgc
     9121 tatacgaagt tattaggtct agagatctga attcgagctc gtttaaactg gatggcggcg
     9181 ttagtatcga atcgacagca gtatagcgac cagcattcac atacgattga cgcatgatat
     9241 tactttctgc gcacttaact tcgcatctgg gcagatgatg tcgaggcgaa aaaaaatata
     9301 aatcacgcta acatttgatt aaaatagaac aactacaata taaaaaaact atacaaatga
     9361 caagttcttg aaaacaagaa tctttttatt tcagtaccta ttaagccaag atttccttga
     9421 cagccttggc gatagcatcg ccaacctcag tggtagagtt agaaccacca aggtcaccgg
     9481 ttctgacacc tgcatccaag acatttctaa cagcttcttc aagagcccta ccttcttcaa
     9541 ccaaatccaa ggataacttc aacatcatag ctgcagataa gatggtagca attgggttaa
     9601 ccttgtttgc tggtaaatct ggggcagaac catgacatgg ttcgtacaaa ccgaatgcct
     9661 tgttagtgtc aggtagggaa gctagagatg cagaaggtaa taaacccaaa gaacctggaa
     9721 taacagaggc ttcatcggag ataatatcac caaacatgtt gttggtaata acaacaccgt
     9781 ttagcttagt tggtgattta accaaaatca tagcagcaga gtcgatcaat tggtgctgaa
     9841 cagttaattg tgggaactca gtcttgatgg tttcttcaac agtctttctc cacaatctgg
     9901 aagaggcaag cacgttagcc ttgtcaagag accagattgg taatggtggg ttttgttgca
     9961 atgccaagaa agcagccatt cttgtaattc tttgaacttc aggaacactg tatttctcag
    10021 agtcccaagc aactccgtca ccttcatctt cttttctttc accaaagtag ataccaccaa
    10081 ccaattctct aacgacgacg aaatcggtac cctttgcata ttcaggcttc aaaggagaaa
    10141 gatctagtaa agaatcagaa gcaaagttac atggtctcaa gttggcgtat agacccaatt
    10201 cctttctgat cttcaataga ccttgttctg gtctaactgc gcccgtaccc cattttggac
    10261 caccaacagc acctagtaag acagcatcgg ctttcttaga ggcttctaga gcttcatctg
    10321 gtaaaggagt gccagtggca tcgatggcag cgcccccgat caagtgatgt tggaaattga
    10381 acttaatttc tggacggact tcagcaatgg cattcaagac cttaatagct tcgtcagtaa
    10441 cttctttacc gacgtgatca cccggtagga caacgatatt cttagacatg gttgtttatg
    10501 ttcggatgtg atgtgagaac tgtatcctag caagatttta aaaggaagta tatgaaagaa
    10561 gaacctcagt ggcaaatcct aaccttttat atttctctac aggggcgcgg cgtggggaca
    10621 attcaacgcg tctgtgaggg gagcgtttcc ctgctcgcag gtctgcagcg aggagccgta
    10681 atttttgctt cgcgccgtgc ggccatcaaa atgtatggat gcaaatgatt atacatgggg
    10741 atgtatgggc taaatgtacg ggcgacagtc acatcatgcc cctgagctgc gcacgtcaag
    10801 actgtcaagg agggtattct gggcctccat gtcgctggcc gggtgacccg gcggggacga
    10861 ggcaagcttc tcgagaaccc ttaatataac ttcgtataat gtatgctata cgaagttatt
    10921 aggtgatatc agatccacta gtggcctatg cattgcatga agaatctgct tagggttagg
    10981 cgttttgcgc tgcttcgcga tgtacgggcc agatatacgc gttgacattg attattgact
    11041 agttattaat agtaatcaat tacggggtca ttagttcata gcccatatat ggagttccgc
    11101 gttacataac ttacggtaaa tggcccgcct ggctgaccgc ccaacgaccc ccgcccattg
    11161 acgtcaataa tgacgtatgt tcccatagta acgccaatag ggactttcca ttgacgtcaa
    11221 tgggtggagt atttacggta aactgcccac ttggcagtac atcaagtgta tcatatgcca
    11281 agtacgcccc ctattgacgt caatgacggt aaatggcccg cctggcatta tgcccagtac
    11341 atgaccttat gggactttcc tacttggcag tacatctacg tattagtcat cgctattacc
    11401 atggtgatgc ggttttggca gtacatcaat gggcgtggat agcggtttga ctcacgggga
    11461 tttccaagtc tccaccccat tgacgtcaat gggagtttgt tttggcacca aaatcaacgg
    11521 gactttccaa aatgtcgtaa caactccgcc ccattgacgc aaatgggcgg taggcgtgta
    11581 cggtgggagg tctatataag cagcgcgttt tgcctgtact gggtctctct ggttagacca
    11641 gatctgagcc tgggagctct ctggctaact agggaaccca ctgcttaagc ctcaataaag
    11701 cttgccttga gtgcttcaag tagtgtgtgc ccgtctgttg tgtgactctg gtaactagag
    11761 atccctcaga cccttttagt cagtgtggaa aatctctagc agtggcgccc gaacagggac
    11821 ttgaaagcga aagggaaacc agaggagctc tctcgacgca ggactcggct tgctgaagcg
    11881 cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt gactagcgga
    11941 ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag aattagatcg
    12001 cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt aaaacatata
    12061 gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt agaaacatca
    12121 gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg atcagaagaa
    12181 cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag gatagagata
    12241 aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag taagaccacc
    12301 gcacagcaag cggccgctga tcttcagacc tggaggagga gatatgaggg acaattggag
    12361 aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag cacccaccaa
    12421 ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag ctttgttcct
    12481 tgggttcttg ggagcagcag gaagcactat gggcgcagcg tcaatgacgc tgacggtaca
    12541 ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga gggctattga
    12601 ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc aggcaagaat
    12661 cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg gttgctctgg
    12721 aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata aatctctgga
    12781 acagatttgg aatcacacga cctggatgga gtgggacaga gaaattaaca attacacaag
    12841 cttaatacac tccttaattg aagaatcgca aaaccagcaa gaaaagaatg aacaagaatt
    12901 attggaatta gataaatggg caagtttgtg gaattggttt aacataacaa attggctgtg
    12961 gtatataaaa ttattcataa tgatagtagg aggcttggta ggtttaagaa tagtttttgc
    13021 tgtactttct atagtgaata gagttaggca gggatattca ccattatcgt ttcagaccca
    13081 cctcccaacc ccgaggggac ccgacaggcc cgaaggaata gaagaagaag gtggagagag
    13141 agacagagac agatccattc gattagtgaa cggatcggca ctgcgtgcgc caattctgca
    13201 gacaaatggc agtattcatc cacaatttta aaagaaaagg ggggattggg gggtacagtg
    13261 caggggaaag aatagtagac ataatagcaa cagacataca aactaaagaa ttacaaaaac
    13321 aaattacaaa aattcaaaat tttcgggttt attacaggga cagcagagat ccagtttggt
    13381 taattaacat t
//
