LOCUS       sequence-461832-        9732 bp    DNA     circular SYN 15-APR-2025
DEFINITION  Express split-RhoA fragments that are fused with CIDs and FPs.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9732)
  TITLE     Stains Lab split-GTPase Plasmids
REFERENCE   2  (bases 1 to 9732)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: 
            pIRES-mCerulean-ABI-RhoA/N12-RhoA/13C(T19N)-PYL-mVenus-CAAX
FEATURES             Location/Qualifiers
     source          1..9732
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        138..517
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        518..729
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     680..700
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     726..750
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     intron          890..1022
                     /label=chimeric intron
                     /note="chimera between introns from human beta-globin and 
                     immunoglobulin heavy chain genes"
     primer_bind     1005..1024
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     promoter        1067..1085
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             join(1091..1093,1094..1096,1097..1807)
                     /codon_start=1
                     /product="enhanced monomeric variant of CFP (Rizzo et al., 
                     2004)"
                     /label=mCerulean
                     /note=" mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTWGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNAISDNVYITADKQKNGIK
                     ANFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     misc_feature    join(2878..3452,3453..3455,3456..3464)
                     /label=IRES2
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(3045..3062)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     3272..3291
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             join(4631..4633,4634..5344)
                     /codon_start=1
                     /product="monomeric optimized variant of ECFP (Kremers et 
                     al., 2006)"
                     /label=SCFP3A
                     /note="mammalian codon-optimized"
                     /translation="VSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTLTWGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYITADKQKNGIKA
                     NFKIRHNIEDGGVQLADHYQQNTPIGDGPVLLPDNHYLSYQSKLSKDPNEKRDHMVLLE
                     FVTAAGITLGMDELYK"
     CDS             join(4631..4633,4634..5344)
                     /codon_start=1
                     /product="strongly enhanced green fluorescent protein 
                     (Kremers et al., 2007)"
                     /label=SGFP2
                     /note="mammalian codon-optimized"
                     /translation="VSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTLTWGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYITADKQKNGIKA
                     NFKIRHNIEDGGVQLADHYQQNTPIGDGPVLLPDNHYLSYQSKLSKDPNEKRDHMVLLE
                     FVTAAGITLGMDELYK"
     polyA_signal    5436..5557
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5447..5466)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     5501..5520
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     rep_origin      5743..6198
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5830..5849)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     6040..6061
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        6315..6672
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     enhancer        6315..6506
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     complement(6338..6358)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      6523..6658
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             6723..7517
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(6777..6796)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     7387..7406
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    7581..7629
                     /note="synthetic polyadenylation signal"
     primer_bind     complement(7670..7689)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     7789..7811
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(7849..7867)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        7935..8039
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(8040..8108,8109..8900)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(8258..8277)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      9071..9659
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     9560..9579
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 tcaatattgg ccattagcca tattattcat tggttatata gcataaatca atattggcta
       61 ttggccattg catacgttgt atctatatca taatatgtac atttatattg gctcatgtcc
      121 aatatgaccg ccatgttggc attgattatt gactagttat taatagtaat caattacggg
      181 gtcattagtt catagcccat atatggagtt ccgcgttaca taacttacgg taaatggccc
      241 gcctggctga ccgcccaacg acccccgccc attgacgtca ataatgacgt atgttcccat
      301 agtaacgcca atagggactt tccattgacg tcaatgggtg gagtatttac ggtaaactgc
      361 ccacttggca gtacatcaag tgtatcatat gccaagtccg ccccctattg acgtcaatga
      421 cggtaaatgg cccgcctagc attatgccca gtacatgacc ttacgggact ttcctacttg
      481 gcagtacatc tacgtattag tcatcgctat taccatggtg atgcggtttt ggcagtacac
      541 caatgggcgt ggatagcggt ttgactcacg gggatttcca agtctccacc ccattgacgt
      601 caatgggagt ttgttttggc accaaaatca acgggacttt ccaaaatgtc gtaacaactg
      661 cgatcgcccg ccccgttgac gcaaatgggc ggtaggcgtg tacggtggga ggtctatata
      721 agcagagctc gtttagtgaa ccgtcagatc actagaagct ttattgcggt agtttatcac
      781 agttaaattg ctaacgcagt cagtgcttct gacacaacag tctcgaactt aagctgcagt
      841 gactctctta aggtagcctt gcagaagttg gtcgtgaggc actgggcagg taagtatcaa
      901 ggttacaaga caggtttaag gagaccaata gaaactgggc ttgtcgagac agagaagact
      961 cttgcgtttc tgataggcac ctattggtct tactgacatc cactttgcct ttctctccac
     1021 aggtgtccac tcccagttca attacagctc ttaaggctag agtacttaat acgactcact
     1081 ataggctagc atggtgagca agggcgagga gctgttcacc ggggtggtgc ccatcctggt
     1141 cgagctggac ggcgacgtaa acggccacaa gttcagcgtg tccggcgagg gcgagggcga
     1201 tgccacctac ggcaagctga ccctgaagtt catctgcacc accggcaagc tgcccgtgcc
     1261 ctggcccacc ctcgtgacca ccctgacctg gggcgtgcag tgcttcgccc gctaccccga
     1321 ccacatgaag cagcacgact tcttcaagtc cgccatgccc gaaggctacg tccaggagcg
     1381 caccatcttc ttcaaggacg acggcaacta caagacccgc gccgaggtga agttcgaggg
     1441 cgacaccctg gtgaaccgca tcgagctgaa gggcatcgac ttcaaggagg acggcaacat
     1501 cctggggcac aagctggagt acaacgccat cagcgacaac gtctatatca ccgccgacaa
     1561 gcagaagaac ggcatcaagg ccaacttcaa gatccgccac aacatcgagg acggcagcgt
     1621 gcagctcgcc gaccactacc agcagaacac ccccatcggc gacggccccg tgctgctgcc
     1681 cgacaaccac tacctgagca cccagtccaa gctgagcaaa gaccccaacg agaagcgcga
     1741 tcacatggtc ctgctggagt tcgtgaccgc cgccgggatc actctcggca tggacgagct
     1801 gtacaagggc ggtggctcat ctggcggagg tgtgcctttg tatggtttta cttcgatttg
     1861 tggaagaaga cctgagatgg aagctgctgt ttcgactata ccaagattcc ttcaatcttc
     1921 ctctggttcg atgttagatg gtcggtttga tcctcaatcc gccgctcatt tcttcggtgt
     1981 ttacgacggc catggcggtt ctcaggtagc gaactattgt agagagagga tgcatttggc
     2041 tttggcggag gagatagcta aggagaaacc gatgctctgc gatggtgata cgtggctgga
     2101 gaagtggaag aaagctcttt tcaactcgtt cctgagagtt gactcggaga ttgagtcagt
     2161 tgcgccggag acggttgggt caacgtcggt ggttgccgtt gttttcccgt ctcacatctt
     2221 cgtcgctaac tgcggtgact ctagagccgt tctttgccgc ggcaaaactg cacttccatt
     2281 atccgttgac cataaaccgg atagagaaga tgaagctgcg aggattgaag ccgcaggagg
     2341 gaaagtgatt cagtggaatg gagctcgtgt tttcggtgtt ctcgccatgt cgagatccat
     2401 tggcgataga tacttgaaac catccatcat tcctgatccg gaagtgacgg ctgtgaagag
     2461 agtaaaagaa gatgattgtc tgattttggc gagtgacggg gtttgggatg taatgacgga
     2521 tgaagaagcg tgtgagatgg caaggaagcg gattctcttg tggcacaaga aaaacgcggt
     2581 ggctggggat gcatcgttgc tcgcggatga gcggagaaag gaagggaaag atcctgcggc
     2641 gatgtccgcg gctgagtatt tgtcaaagct ggcgatacag agaggaagca aagacaacat
     2701 aagtgtggtg gtggttgatt tgaagcagat ctcgtacgcg tcccggggcg gtggctcatc
     2761 tggcggaggt gagctcgctg ccatccggaa gaaactggtg attgttggtg atggagccta
     2821 agaattcacg cgtcgagcat gcatctaggg cggccaattc cgcccctctc cccccccccc
     2881 ctctccctcc ccccccccta acgttactgg ccgaagccgc ttggaataag gccggtgtgc
     2941 gtttgtctat atgttatttt ccaccatatt gccgtctttt ggcaatgtga gggcccggaa
     3001 acctggccct gtcttcttga cgagcattcc taggggtctt tcccctctcg ccaaaggaat
     3061 gcaaggtctg ttgaatgtcg tgaaggaagc agttcctctg gaagcttctt gaagacaaac
     3121 aacgtctgta gcgacccttt gcaggcagcg gaacccccca cctggcgaca ggtgcctctg
     3181 cggccaaaag ccacgtgtat aagatacacc tgcaaaggcg gcacaacccc agtgccacgt
     3241 tgtgagttgg atagttgtgg aaagagtcaa atggctctcc tcaagcgtat tcaacaaggg
     3301 gctgaaggat gcccagaagg taccccattg tatgggatct gatctggggc ctcggtgcac
     3361 atgctttaca tgtgtttagt cgaggttaaa aaaacgtcta ggccccccga accacgggga
     3421 cgtggttttc ctttgaaaaa cacgatgata agcttgccac aacccgggat tctctagagt
     3481 cgacatgtgt ggaaagaact gcttgctcat agtcttcagc aaggaccagt tcccagaggt
     3541 gtatgtgccc acagtgtttg agaactatgt ggcagatatc gaggtggatg gaaagcaggt
     3601 agagttggct ttgtgggaca cagctgggca ggaagattat gatcgcctga ggcccctctc
     3661 ctacccagat accgatgtta tactgatgtg tttttccatc gacagccctg atagtttaga
     3721 aaacatccca gaaaagtgga ccccagaagt caagcatttc tgtcccaacg tgcccatcat
     3781 cctggttggg aataagaagg atcttcggaa tgatgagcac acaaggcggg agctagccaa
     3841 gatgaagcag gagccggtga aacctgaaga aggcagagat atggcaaaca ggattggcgc
     3901 ttttgggtac atggagtgtt cagcaaagac caaagatgga gtgagagagg tttttgaaat
     3961 ggctacgaga gctgctctgc aagctgggga tcccaattgg gagctcgtgt acacggcgcg
     4021 cctgcaggga ggtggctcat ctggcggagg tcagatctcg tacgcgtccc ggggcactca
     4081 agacgaattc acccaactct cccaatcaat cgccgagttc cacacgtacc aactcggtaa
     4141 cggccgttgc tcatctctcc tagctcagcg aatccacgcg ccgccggaaa cagtatggtc
     4201 cgtggtgaga cgtttcgata ggccacagat ttacaaacac ttcatcaaaa gctgtaacgt
     4261 gagtgaagat ttcgagatgc gagtgggatg cacgcgcgac gtgaacgtga taagtggatt
     4321 accggcgaat acgtctcgag agagattaga tctgttggac gatgatcgga gagtgactgg
     4381 gtttagtata accggtggtg aacataggct gaggaattat aaatcggtta cgacggttca
     4441 tagatttgag aaagaagaag aagaagaaag gatctggacc gttgttttgg aatcttatgt
     4501 tgttgatgta ccggaaggta attcggagga agatacgaga ttgtttgctg atacggttat
     4561 tagattgaat cttcagaaac ttgcttcgat tactgaagct atgaacggcg gtggctcatc
     4621 tggcggaggt gtgagcaagg gcgaggagct gttcaccggg gtggtgccca tcctggtcga
     4681 gctggacggc gacgtaaacg gccacaagtt cagcgtgtcc ggcgagggcg agggcgatgc
     4741 cacctacggc aagctgaccc tgaagttcat ctgcaccacc ggcaagctgc ccgtgccctg
     4801 gcccaccctc gtgaccaccc tgacctgggg cgtgcagtgc ttcgcccgct accccgacca
     4861 catgaagcag cacgacttct tcaagtccgc catgcccgaa ggctacgtcc aggagcgcac
     4921 catcttcttc aaggacgacg gcaactacaa gacccgcgcc gaggtgaagt tcgagggcga
     4981 caccctggtg aaccgcatcg agctgaaggg catcgacttc aaggaggacg gcaacatcct
     5041 ggggcacaag ctggagtaca actacaacag ccacaacgtc tatatcaccg ccgacaagca
     5101 gaagaacggc atcaaggcca acttcaagat ccgccacaac atcgaggacg gcggcgtgca
     5161 gctcgccgac cactaccagc agaacacccc catcggcgac ggccccgtgc tgctgcccga
     5221 caaccactac ctgagctacc agtccaagct gagcaaagac cccaacgaga agcgcgatca
     5281 catggtcctg ctggagttcg tgaccgccgc cgggatcact ctcggcatgg acgagctgta
     5341 caagaaaaaa aagaagaaaa agagcaagac caaatgcgtg attatgtaag cggccgcttc
     5401 cctttagtga gggttaatgc ttcgagcaga catgataaga tacattgatg agtttggaca
     5461 aaccacaact agaatgcagt gaaaaaaatg ctttatttgt gaaatttgtg atgctattgc
     5521 tttatttgta accattataa gctgcaataa acaagttaac aacaacaatt gcattcattt
     5581 tatgtttcag gttcaggggg agatgtggga ggttttttaa agcaaataaa acctctacaa
     5641 atgtggtaaa atccgataag gatcgatccg ggctggcgta atagcgaaga ggcccgcacc
     5701 gatcgccctt cccaacagtt gcgcagcctg aatggcgaat ggacgcgccc tgtagcggcg
     5761 cattaagcgc ggcgggtgtg gtggttacgc gcagcgtgac cgctacactt gccagcgccc
     5821 tagcgcccgc tcctttcgct ttcttccctt cctttctcgc cacgttcgcc ggctttcccc
     5881 gtcaagctct aaatcggggg ctccctttag ggttccgatt tagtgcttta cggcacctcg
     5941 accccaaaaa acttgattag ggtgatggtt cacgtagtgg gccatcgccc tgatagacgg
     6001 tttttcgccc tttgacgttg gagtccacgt tctttaatag tggactcttg ttccaaactg
     6061 gaacaacact caaccctatc tcggtctatt cttttgattt ataagggatt ttgccgattt
     6121 cggcctattg gttaaaaaat gagctgattt aacaaaaatt taacgcgaat tttaacaaaa
     6181 tattaacgct tacaatttcc tgatgcggta ttttctcctt acgcatctgt gcggtatttc
     6241 acaccgcata cgcggatctg cgcagcacca tggcctgaaa taacctctga aagaggaact
     6301 tggttaggta ccttctgagg cggaaagaac cagctgtgga atgtgtgtca gttagggtgt
     6361 ggaaagtccc caggctcccc agcaggcaga agtatgcaaa gcatgcatct caattagtca
     6421 gcaaccaggt gtggaaagtc cccaggctcc ccagcaggca gaagtatgca aagcatgcat
     6481 ctcaattagt cagcaaccat agtcccgccc ctaactccgc ccatcccgcc cctaactccg
     6541 cccagttccg cccattctcc gccccatggc tgactaattt tttttattta tgcagaggcc
     6601 gaggccgcct cggcctctga gctattccag aagtagtgag gaggcttttt tggaggccta
     6661 ggcttttgca aaaagcttga ttcttctgac acaacagtct cgaacttaag gctagagcca
     6721 ccatgattga acaagatgga ttgcacgcag gttctccggc cgcttgggtg gagaggctat
     6781 tcggctatga ctgggcacaa cagacaatcg gctgctctga tgccgccgtg ttccggctgt
     6841 cagcgcaggg gcgcccggtt ctttttgtca agaccgacct gtccggtgcc ctgaatgaac
     6901 tgcaggacga ggcagcgcgg ctatcgtggc tggccacgac gggcgttcct tgcgcagctg
     6961 tgctcgacgt tgtcactgaa gcgggaaggg actggctgct attgggcgaa gtgccggggc
     7021 aggatctcct gtcatctcac cttgctcctg ccgagaaagt atccatcatg gctgatgcaa
     7081 tgcggcggct gcatacgctt gatccggcta cctgcccatt cgaccaccaa gcgaaacatc
     7141 gcatcgagcg agcacgtact cggatggaag ccggtcttgt cgatcaggat gatctggacg
     7201 aagagcatca ggggctcgcg ccagccgaac tgttcgccag gctcaaggcg cgcatgcccg
     7261 acggcgagga tctcgtcgtg acccatggcg atgcctgctt gccgaatatc atggtggaaa
     7321 atggccgctt ttctggattc atcgactgtg gccggctggg tgtggcggac cgctatcagg
     7381 acatagcgtt ggctacccgt gatattgctg aagagcttgg cggcgaatgg gctgaccgct
     7441 tcctcgtgct ttacggtatc gccgctcccg attcgcagcg catcgccttc tatcgccttc
     7501 ttgacgagtt cttctgagcg ggactctggg gttcgaaatg accgaccaag cgacgcccaa
     7561 cctgccatca cgatggccgc aataaaatat ctttattttc attacatctg tgtgttggtt
     7621 ttttgtgtga atcgatagcg ataaggatcc gcgtatggtg cactctcagt acaatctgct
     7681 ctgatgccgc atagttaagc cagccccgac acccgccaac acccgctgac gcgccctgac
     7741 gggcttgtct gctcccggca tccgcttaca gacaagctgt gaccgtctcc gggagctgca
     7801 tgtgtcagag gttttcaccg tcatcaccga aacgcgcgag acgaaagggc ctcgtgatac
     7861 gcctattttt ataggttaat gtcatgataa taatggtttc ttagacgtca ggtggcactt
     7921 ttcggggaaa tgtgcgcgga acccctattt gtttattttt ctaaatacat tcaaatatgt
     7981 atccgctcat gagacaataa ccctgataaa tgcttcaata atattgaaaa aggaagagta
     8041 tgagtattca acatttccgt gtcgccctta ttcccttttt tgcggcattt tgccttcctg
     8101 tttttgctca cccagaaacg ctggtgaaag taaaagatgc tgaagatcag ttgggtgcac
     8161 gagtgggtta catcgaactg gatctcaaca gcggtaagat ccttgagagt tttcgccccg
     8221 aagaacgttt tccaatgatg agcactttta aagttctgct atgtggcgcg gtattatccc
     8281 gtattgacgc cgggcaagag caactcggtc gccgcataca ctattctcag aatgacttgg
     8341 ttgagtactc accagtcaca gaaaagcatc ttacggatgg catgacagta agagaattat
     8401 gcagtgctgc cataaccatg agtgataaca ctgcggccaa cttacttctg acaacgatcg
     8461 gaggaccgaa ggagctaacc gcttttttgc acaacatggg ggatcatgta actcgccttg
     8521 atcgttggga accggagctg aatgaagcca taccaaacga cgagcgtgac accacgatgc
     8581 ctgtagcaat ggcaacaacg ttgcgcaaac tattaactgg cgaactactt actctagctt
     8641 cccggcaaca attaatagac tggatggagg cggataaagt tgcaggacca cttctgcgct
     8701 cggcccttcc ggctggctgg tttattgctg ataaatctgg agccggtgag cgtgggtctc
     8761 gcggtatcat tgcagcactg gggccagatg gtaagccctc ccgtatcgta gttatctaca
     8821 cgacggggag tcaggcaact atggatgaac gaaatagaca gatcgctgag ataggtgcct
     8881 cactgattaa gcattggtaa ctgtcagacc aagtttactc atatatactt tagattgatt
     8941 taaaacttca tttttaattt aaaaggatct aggtgaagat cctttttgat aatctcatga
     9001 ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc agaccccgta gaaaagatca
     9061 aaggatcttc ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac
     9121 caccgctacc agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg
     9181 taactggctt cagcagagcg cagataccaa atactgttct tctagtgtag ccgtagttag
     9241 gccaccactt caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac
     9301 cagtggctgc tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt
     9361 taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg
     9421 agcgaacgac ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc
     9481 ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc
     9541 gcacgaggga gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc
     9601 acctctgact tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa
     9661 acgccagcaa cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgg
     9721 ctcgacagat ct
//
