LOCUS       sequence-462881-       14266 bp    DNA     circular SYN 15-APR-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 14266)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 14266)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF0657
FEATURES             Location/Qualifiers
     source          1..14266
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        5..231
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             232..412
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    459..584
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1077..1310
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1495..1539
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        1770..1980
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     promoter        1792..1988
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      1839..1974
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             2020..2622
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGAS"
     primer_bind     complement(2020..2039)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     2516..2536
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    2665..2782
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        2920..4098
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          3151..4089
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     4046..4066
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     misc_feature    10123..10711
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(10176..10196)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(10594..10605)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             10783..11016
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    11094..11215
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(11131..11150)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     11185..11204
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      11255..11390
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(11410..11429)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(11411..11429)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(11439..11456)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(11439..11455)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(11448..11470)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      11597..12052
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(11684..11703)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     11894..11915
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        12078..12182
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(12183..12251,12252..13043)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(12401..12420)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      13214..13802
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     13703..13722
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     13956..13973
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    14090..14111
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(14126..14131,14132..14149,14150..14156)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    14164..14180
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     14169..14191
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     14188..14204
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     14188..14204
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     14223..14243
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        14225..14243
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
ORIGIN
        1 ttaatgtagt cttatgcaat actcttgtag tcttgcaaca tggtaacgat gagttagcaa
       61 catgccttac aaggagagaa aaagcaccgt gcatgccgat tggtggaagt aaggtggtac
      121 gatcgtgcct tattaggaag gcaacagacg ggtctgacat ggattggacg aaccactgaa
      181 ttgccgcatt gcagagatat tgtatttaag tgcctagctc gatacataaa cgggtctctc
      241 tggttagacc agatctgagc ctgggagctc tctggctaac tagggaaccc actgcttaag
      301 cctcaataaa gcttgccttg agtgcttcaa gtagtgtgtg cccgtctgtt gtgtgactct
      361 ggtaactaga gatccctcag acccttttag tcagtgtgga aaatctctag cagtggcgcc
      421 cgaacaggga cttgaaagcg aaagggaaac cagaggagct ctctcgacgc aggactcggc
      481 ttgctgaagc gcgcacggca agaggcgagg ggcggcgact ggtgagtacg ccaaaaattt
      541 tgactagcgg aggctagaag gagagagatg ggtgcgagag cgtcagtatt aagcggggga
      601 gaattagatc gcgatgggaa aaaattcggt taaggccagg gggaaagaaa aaatataaat
      661 taaaacatat agtatgggca agcagggagc tagaacgatt cgcagttaat cctggcctgt
      721 tagaaacatc agaaggctgt agacaaatac tgggacagct acaaccatcc cttcagacag
      781 gatcagaaga acttagatca ttatataata cagtagcaac cctctattgt gtgcatcaaa
      841 ggatagagat aaaagacacc aaggaagctt tagacaagat agaggaagag caaaacaaaa
      901 gtaagaccac cgcacagcaa gcggccgctg atcttcagac ctggaggagg agatatgagg
      961 gacaattgga gaagtgaatt atataaatat aaagtagtaa aaattgaacc attaggagta
     1021 gcacccacca aggcaaagag aagagtggtg cagagagaaa aaagagcagt gggaatagga
     1081 gctttgttcc ttgggttctt gggagcagca ggaagcacta tgggcgcagc gtcaatgacg
     1141 ctgacggtac aggccagaca attattgtct ggtatagtgc agcagcagaa caatttgctg
     1201 agggctattg aggcgcaaca gcatctgttg caactcacag tctggggcat caagcagctc
     1261 caggcaagaa tcctggctgt ggaaagatac ctaaaggatc aacagctcct ggggatttgg
     1321 ggttgctctg gaaaactcat ttgcaccact gctgtgcctt ggaatgctag ttggagtaat
     1381 aaatctctgg aacagatttg gaatcacacg acctggatgg agtgggacag agaaattaac
     1441 aattacacaa gcttaataca ctccttaatt gaagaatcgc aaaaccagca agaaaagaat
     1501 gaacaagaat tattggaatt agataaatgg gcaagtttgt ggaattggtt taacataaca
     1561 aattggctgt ggtatataaa attattcata atgatagtag gaggcttggt aggtttaaga
     1621 atagtttttg ctgtactttc tatagtgaat agagttaggc agggatattc accattatcg
     1681 tttcagaccc acctcccaac cccgagggga cccatgcatt gcatctcaat tagtcagcaa
     1741 ccaggtgtgg aaagtcccca ggctccccag caggcagaag tatgcaaagc atgcgtctca
     1801 attagtcagc aaccatagtc ccgcccctaa ctccgcccat cccgccccta actccgccca
     1861 gttccgccca ttctccgccc catggctgac taattttttt tatttatgca gaggccgagg
     1921 ccgcctcggc ctctgagcta ttccagaagt agtgaggagg cttttttgga ggcctaggct
     1981 tttgcaaaaa gctttctaga ggtaccaccg gagcttacca tgaccgagta caagcccacg
     2041 gtgcgcctcg ccacccgcga cgacgtcccc agggccgtac gcaccctcgc cgccgcgttc
     2101 gccgactacc ccgccacgcg ccacaccgtc gatccggacc gccacatcga gcgggtcacc
     2161 gagctgcaag aactcttcct cacgcgcgtc gggctcgaca tcggcaaggt gtgggtcgcg
     2221 gacgacggcg ccgccgtggc ggtctggacc acgccggaga gcgtcgaagc gggggcggtg
     2281 ttcgccgaga tcggcccgcg catggccgag ttgagcggtt cccggctggc cgcgcagcaa
     2341 cagatggaag gcctcctggc gccgcaccgg cccaaggagc ccgcgtggtt cctggccacc
     2401 gtcggcgtct cgcccgacca ccagggcaag ggtctgggca gcgccgtcgt gctccccgga
     2461 gtggaggcgg ccgagcgcgc cggggtgccc gccttcctgg agacctccgc gccccgcaac
     2521 ctccccttct acgagcggct cggcttcacc gtcaccgccg acgtcgaggt gcccgaagga
     2581 ccgcgcacct ggtgcatgac ccgcaagccc ggtgccagtt aacgaattct agatcttgag
     2641 acaaatggca gtattcatcc acaattttaa aagaaaaggg gggattgggg ggtacagtgc
     2701 aggggaaaga atagtagaca taatagcaac agacatacaa actaaagaat tacaaaaaca
     2761 aattacaaaa attcaaaatt ttcgggttta ttacagggac agcagagatc cactttggcg
     2821 ccggctcgag gcctgcaggt gcaaagatgg ataaagtttt aaacagagag gaatctttgc
     2881 agctaatgga ccttctaggt cttgaaagga gtgggaattg gctccggtgc ccgtcagtgg
     2941 gcagagcgca catcgcccac agtccccgag aagttggggg gaggggtcgg caattgaacc
     3001 ggtgcctaga gaaggtggcg cggggtaaac tgggaaagtg atgtcgtgta ctggctccgc
     3061 ctttttcccg agggtggggg agaaccgtat ataagtgcag tagtcgccgt gaacgttctt
     3121 tttcgcaacg ggtttgccgc cagaacacag gtaagtgccg tgtgtggttc ccgcgggcct
     3181 ggcctcttta cgggttatgg cccttgcgtg ccttgaatta cttccacctg gctgcagtac
     3241 gtgattcttg atcccgagct tcgggttgga agtgggtggg agagttcgag gccttgcgct
     3301 taaggagccc cttcgcctcg tgcttgagtt gaggcctggc ctgggcgctg gggccgccgc
     3361 gtgcgaatct ggtggcacct tcgcgcctgt ctcgctgctt tcgataagtc tctagccatt
     3421 taaaattttt gatgacctgc tgcgacgctt tttttctggc aagatagtct tgtaaatgcg
     3481 ggccaagatc tgcacactgg tatttcggtt tttggggccg cgggcggcga cggggcccgt
     3541 gcgtcccagc gcacatgttc ggcgaggcgg ggcctgcgag cgcggccacc gagaatcgga
     3601 cgggggtagt ctcaagctgg ccggcctgct ctggtgcctg gcctcgcgcc gccgtgtatc
     3661 gccccgccct gggcggcaag gctggcccgg tcggcaccag ttgcgtgagc ggaaagatgg
     3721 ccgcttcccg gccctgctgc agggagctca aaatggagga cgcggcgctc gggagagcgg
     3781 gcgggtgagt cacccacaca aaggaaaagg gcctttccgt cctcagccgt cgcttcatgt
     3841 gactccacgg agtaccgggc gccgtccagg cacctcgatt agttctcgag cttttggagt
     3901 acgtcgtctt taggttgggg ggaggggttt tatgcgatgg agtttcccca cactgagtgg
     3961 gtggagactg aagttaggcc agcttggcac ttgatgtaat tctccttgga atttgccctt
     4021 tttgagtttg gatcttggtt cattctcaag cctcagacag tggttcaaag tttttttctt
     4081 ccatttcagg tgtcgtgagg ctagcaccgc caccatgaag gcggcagaca ctgtgatcct
     4141 gtgggcaaga agtaaaaatg accagctgag gatttctttt cctccaggac tgtgttgggg
     4201 tgacaggatg cctgataagg atgacattcg gctgctgccg tcagcattgg gtgtgaagaa
     4261 gagaaaacga ggacccaaga agcagaagga gaacaagcca ggaaaacccc gaaaacgcaa
     4321 gaagcgtgac agtgaggagg aatttggttc tgagcgagat gagtaccggg agaagtcaga
     4381 gagtgggggc agtgaatatg gaaccggacc gggtcggaaa cgaagaagga agcaccgaga
     4441 aaaaaaggag aagaagacaa agcggcggaa aaagggggag ggagatgggg ggcaaaagca
     4501 agtggaacag aagtcatcag caactctgct tctgacctgg ggcctggagg atgtggagca
     4561 tgtgttctct gaggaggatt accacacgct caccaactac aaagccttca gccagttcat
     4621 gaggccccta attgctaaga agaatcctaa gatcccaatg tctaagatga tgaccatcct
     4681 tggggccaaa tggagagagt tcagtgccaa caaccccttc aaggggtcag cagctgctgt
     4741 ggcggcggca gcggcagcag cagcagcagc tgtagctgag caggtgtcag ctgctgtctc
     4801 gtcggccacc cccatagcac cctccggacc ccccgccctt ccaccacccc ctgctgctga
     4861 tatccagccc ccacccatcc gaagagccaa aaccaaagag ggcaaaggtc caggccataa
     4921 gaggcggagt aagagccccc gagtgcctga tggacgcaag aagcttcggg gaaagaaaat
     4981 ggcaccactc aaaataaaac tagggcttct gggtggcaag aggaagaaag gaggctcgta
     5041 tgtttttcag agcgacgaag gtcctgaacc agaggctgag gaatcagacc tggacagtgg
     5101 cagtgtccac agtgcctcag gccggcctga tggccctgtc cgcaccaaga aactaaagag
     5161 aggccggcca ggaaggaaga agaagaaggt cctgggctgt cctgcagtgg ccggggagga
     5221 ggaggttgat ggctacgaga cggatcacca ggattactgt gaggtgtgcc agcagggtgg
     5281 ggaaattatt ctgtgtgaca cctgccctcg tgcctaccac ctcgtctgcc ttgatcctga
     5341 gcttgaccgg gctccagagg gcaaatggag ctgccctcac tgtgagaagg agggggtcca
     5401 gtgggaggcc aaggaggaag aagaagaata cgaagaggag ggagaggaag aaggggagaa
     5461 ggaggaggag gatgatcaca tggagtactg ccgcgtatgc aaggacggcg gggagctcct
     5521 gtgctgtgac gcgtgcatct cctcctacca cattcattgt ctaaaccctc ccctgcctga
     5581 cattcccaat ggtgaatggc tgtgtccccg atgcacatgc cccgtgctga agggtcgagt
     5641 gcagaagatc ctacattggc ggtgggggga gccacctgta gcagtgccag cccctcaaca
     5701 ggcagatgga aatccagatg tcccaccccc ccgtcctctt caaggcagat cagagcgaga
     5761 gttctttgtc aagtgggtag gactatccta ctggcactgc tcctgggcca aggagcttca
     5821 gctggaaatc ttccatttgg ttatgtatcg aaactaccag cggaagaatg acatggatga
     5881 gcccccaccc ctggactatg gctccggcga ggatgatggg aagagcgaca agcgtaaagt
     5941 gaaagacccg cactatgctg agatggagga gaagtactat cgttttggca tcaagccaga
     6001 gtggatgacc gtccaccgca tcatcaacca cagtgtggat aaaaagggga attaccacta
     6061 tctagtaaaa tggagggact taccatatga ccagtccacg tgggaggaag atgaaatgaa
     6121 tatccctgaa tacgaagaac ataagcaaag ctactggaga caccgagaac taattatggg
     6181 ggaagaccct gcccagcccc gcaagtataa gaagaagaag aaggagctac agggtgatgg
     6241 gcctcccagt tctcccacta atgatcctac cgtgaaatat gagactcagc cacggtttat
     6301 cacagccact ggaggcaccc tgcacatgta tcagttggaa gggctgaact ggctacgctt
     6361 ctcctgggcc cagggcactg acaccattct agctgatgag atggggctag gcaagaccat
     6421 acaaaccatc gtcttcctct actcactcta caaggagggc cacacaaaag gtcccttcct
     6481 ggtgagtgcc ccactctcta ccatcattaa ctgggagcgg gagttccaga tgtgggcacc
     6541 caaattctat gtggtgacat acacgggtga caaggacagc cgggccatca ttcgtgagaa
     6601 tgaattctcc tttgaggaca atgccatcaa agggggcaag aaagctttta agatgaagag
     6661 ggaggcacag gtgaagttcc atgttctcct gacatcgtat gagctgatca ccattgatca
     6721 ggcagcactt ggttccatcc gctgggcctg tcttgtggta gatgaggccc atcgactcaa
     6781 gaacaaccag tccaagtttt tcagggttct caatggttac aagatagatc ataagttgct
     6841 gctgacagga accccattgc agaataatct ggaggagctc ttccatctcc tgaacttcct
     6901 caccccagag agatttaaca acttggaggg cttcctggag gagtttgctg acatatccaa
     6961 agaggaccag atcaagaaac tgcatgattt gctggggcca cacatgctgc ggagactcaa
     7021 ggcagatgtc tttaagaaca tgccagccaa gacagagctc atcgttcggg tggagctaag
     7081 ccccatgcag aagaaatact acaaatacat cctgactcga aattttgagg ccttgaattc
     7141 acgaggtggt gggaaccagg tgtcgctgct taatatcatg atggatctta agaagtgctg
     7201 caaccatcca tacctttttc ccgtggctgc tatggagtcc cccaaactcc ccagtggggc
     7261 ttatgagggt ggggcactta ttaagtcgtc tgggaagctc atgctgctcc agaagatgct
     7321 gcgaaagctg aaggagcaag gacaccgagt gctcatcttc tcgcagatga ccaaaatgtt
     7381 agacttgctt gaggacttct tagactatga aggctacaag tatgagcgca tcgatggtgg
     7441 tatcacgggt gccctgaggc aggaggccat cgatcggttt aatgctcctg gggcccaaca
     7501 attctgcttc ctcctgtcca cccgagctgg gggcctgggc atcaatctgg ccactgctga
     7561 cactgtcatc atctttgatt ctgactggaa cccccataat gacatccagg cctttagccg
     7621 ggctcatcgg attggccagg ccaacaaagt gatgatttac cggtttgtga ctcgcgcgtc
     7681 agtggaagag cgaatcacac aagtggccaa gagaaagatg atgctgacac acctggttgt
     7741 gcggcctggg ctgggctcca aggcaggctc catgtccaag caggagcttg acgacattct
     7801 caaatttggc actgaagagc tattcaagga tgaaaacgag ggggagaaca aggaggagga
     7861 cagcagtgtg attcattatg acaatgaggc catcgctcgg ctgttggacc ggaaccagga
     7921 tgcaactgag gacactgacg tgcagaacat gaatgagtat ctcagctcct tcaaggtggc
     7981 acagtacgtc gtgcgggaag aagacaagat tgaggaaatt gagcgagaga tcatcaagca
     8041 ggaggagaat gtggaccctg actactggga gaagctgctg aggcatcact atgagcaaca
     8101 gcaggaagac ctagcccgga atctaggcaa gggcaagcgg gttcgcaagc aagttaacta
     8161 caatgatgct gctcaggaag accaagacaa ccagtcagag tactcggtgg gttcagagga
     8221 ggaggatgaa gacttcgatg aacgtcctga agggcgtaga cagtcaaaga ggcagctccg
     8281 gaatgagaaa gataagccac tgcctccact gctggcccga gtcgggggca acattgaggt
     8341 gctgggcttc aacacccgtc agcggaaggc tttcctcaat gctgtgatgc gctgggggat
     8401 gccaccacag gatgccttca ccacacagtg gctggtgcgg gacctgaggg gcaagactga
     8461 gaaggagttt aaggcctatg tgtctttgtt catgcgccat ctgtgtgagc ctggggcaga
     8521 cggctctgaa acctttgccg atggggtccc tcgggaggga ctgagtcgcc agcaggtgtt
     8581 gacccgcatt ggagtcatgt ctctcgtcaa aaagaaggtg caggagtttg agcacatcaa
     8641 tgggcgttgg tcaatgccgg aactgatgcc tgaccccagc gccgattcta agcgctcctc
     8701 cagagcctcc tctcctacca aaacgtctcc caccactcct gaggcttctg ctaccaacag
     8761 tccctgcacc tctaaacctg ctactccagc tccaagtgag aaaggagaag gcataaggac
     8821 acctcttgag aaggaggaag ctgaaaacca ggaggaaaag ccagagaaga acagcagaat
     8881 tggggagaag atggagacag aggctgatgc ccccagccca gccccatcac ttggggagcg
     8941 gctggagcca aggaagattc ctctagagga tgaggtgcca ggggtgcctg gagagatgga
     9001 gcctgaacct gggtaccgtg gggacagaga gaagtcagaa gatgtaaaag gtgaccggga
     9061 gcttcgacca gggcctcgag atgagccacg gtccaatggg cgacgagagg aaaagacaga
     9121 gaagccccgg ttcatgttca atatcgccga tggtggcttc acagagcttc acacactgtg
     9181 gcagaatgag gaacgggcag ctatttcctc ggggaaactc aatgagatct ggcacagaag
     9241 acatgactat tggcttctgg ctgggattgt cctccatggc tatgcacggt ggcaggacat
     9301 ccagaatgat gctcaatttg ccattatcaa cgagccattt aaaactgaag ccaataaggg
     9361 gaactttctg gagatgaaaa ataagttcct ggcccggagg ttcaagctcc tggagcaggc
     9421 gctggtgatt gaggagcagc tgcggcgggc ggcctacctg aacctgtcgc aggagccggc
     9481 gcaccccgcc atggccctcc acgcccgctt cgccgaggcc gagtgcctgg ccgagagcca
     9541 ccagcacctc tccaaggagt cgctggcggg gaacaagccg gccaacgccg tcctgcacaa
     9601 ggttctgaac cagctggagg agttgctgag cgacatgaag gcggacgtga cccgcctgcc
     9661 agccacgctg tcccgaatac cccccatcgc agcccgcctt cagatgtccg agcgcagcat
     9721 cctcagccgg ctggccagca agggcacgga gcctcacccc acaccggcct acccgccggg
     9781 tccctacgct acacctccgg ggtacggggc ggccttcagc gccgcacccg taggggccct
     9841 ggccgccgca ggcgccaatt acagccagat gcctgcaggg tccttcatca cagccgccac
     9901 caacggccct ccagtgcttg tgaagaagga gaaggaaatg gtgggggcat tggtgtcaga
     9961 cgggctggat cggaaggagc cccgagccgg ggaggtgatc tgtatagacg actagtaatg
    10021 aactagtccg taacttgaaa gtatttcgat ttcttggctt tatatatctt gtggaaagga
    10081 cgagacaata gagacagttt gacagacacg cgttaagtcg acaatcaacc tctggattac
    10141 aaaatttgtg aaagattgac tggtattctt aactatgttg ctccttttac gctatgtgga
    10201 tacgctgctt taatgccttt gtatcatgct attgcttccc gtatggcttt cattttctcc
    10261 tccttgtata aatcctggtt gctgtctctt tatgaggagt tgtggcccgt tgtcaggcaa
    10321 cgtggcgtgg tgtgcactgt gtttgctgac gcaaccccca ctggttgggg cattgccacc
    10381 acctgtcagc tcctttccgg gactttcgct ttccccctcc ctattgccac ggcggaactc
    10441 atcgccgcct gccttgcccg ctgctggaca ggggctcggc tgttgggcac tgacaattcc
    10501 gtggtgttgt cggggaaatc atcgtccttt ccttggctgc tcgcctgtgt tgccacctgg
    10561 attctgcgcg ggacgtcctt ctgctacgtc ccttcggccc tcaatccagc ggaccttcct
    10621 tcccgcggcc tgctgccggc tctgcggcct cttccgcgtc ttcgccttcg ccctcagacg
    10681 agtcggatct ccctttgggc cgcctccccg cgtcgacttt aagaccaatg acttacaagg
    10741 cagctgtaga tcttagccac tttttaaaag aaaagggggg actggaaggg ctaattcact
    10801 cccaacgaag acaagatctg ctttttgctt gtactgggtc tctctggtta gaccagatct
    10861 gagcctggga gctctctggc taactaggga acccactgct taagcctcaa taaagcttgc
    10921 cttgagtgct tcaagtagtg tgtgcccgtc tgttgtgtga ctctggtaac tagagatccc
    10981 tcagaccctt ttagtcagtg tggaaaatct ctagcagtac gtatagtagt tcatgtcatc
    11041 ttattattca gtatttataa cttgcaaaga aatgaatatc agagagtgag aggaacttgt
    11101 ttattgcagc ttataatggt tacaaataaa gcaatagcat cacaaatttc acaaataaag
    11161 catttttttc actgcattct agttgtggtt tgtccaaact catcaatgta tcttatcatg
    11221 tctggctcta gctatcccgc ccctaactcc gcccatcccg cccctaactc cgcccagttc
    11281 cgcccattct ccgccccatg gctgactaat tttttttatt tatgcagagg ccgaggccgc
    11341 ctcggcctct gagctattcc agaagtagtg aggaggcttt tttggaggcc tagggacgta
    11401 cccaattcgc cctatagtga gtcgtattac gcgcgctcac tggccgtcgt tttacaacgt
    11461 cgtgactggg aaaaccctgg cgttacccaa cttaatcgcc ttgcagcaca tccccctttc
    11521 gccagctggc gtaatagcga agaggcccgc accgatcgcc cttcccaaca gttgcgcagc
    11581 ctgaatggcg aatgggacgc gccctgtagc ggcgcattaa gcgcggcggg tgtggtggtt
    11641 acgcgcagcg tgaccgctac acttgccagc gccctagcgc ccgctccttt cgctttcttc
    11701 ccttcctttc tcgccacgtt cgccggcttt ccccgtcaag ctctaaatcg ggggctccct
    11761 ttagggttcc gatttagtgc tttacggcac ctcgacccca aaaaacttga ttagggtgat
    11821 ggttcacgta gtgggccatc gccctgatag acggtttttc gccctttgac gttggagtcc
    11881 acgttcttta atagtggact cttgttccaa actggaacaa cactcaaccc tatctcggtc
    11941 tattcttttg atttataagg gattttgccg atttcggcct attggttaaa aaatgagctg
    12001 atttaacaaa aatttaacgc gaattttaac aaaatattaa cgcttacaat ttaggtggca
    12061 cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt tttctaaata cattcaaata
    12121 tgtatccgct catgagacaa taaccctgat aaatgcttca ataatattga aaaaggaaga
    12181 gtatgagtat tcaacatttc cgtgtcgccc ttattccctt ttttgcggca ttttgccttc
    12241 ctgtttttgc tcacccagaa acgctggtga aagtaaaaga tgctgaagat cagttgggtg
    12301 cacgagtggg ttacatcgaa ctggatctca acagcggtaa gatccttgag agttttcgcc
    12361 ccgaagaacg ttttccaatg atgagcactt ttaaagttct gctatgtggc gcggtattat
    12421 cccgtattga cgccgggcaa gagcaactcg gtcgccgcat acactattct cagaatgact
    12481 tggttgagta ctcaccagtc acagaaaagc atcttacgga tggcatgaca gtaagagaat
    12541 tatgcagtgc tgccataacc atgagtgata acactgcggc caacttactt ctgacaacga
    12601 tcggaggacc gaaggagcta accgcttttt tgcacaacat gggggatcat gtaactcgcc
    12661 ttgatcgttg ggaaccggag ctgaatgaag ccataccaaa cgacgagcgt gacaccacga
    12721 tgcctgtagc aatggcaaca acgttgcgca aactattaac tggcgaacta cttactctag
    12781 cttcccggca acaattaata gactggatgg aggcggataa agttgcagga ccacttctgc
    12841 gctcggccct tccggctggc tggtttattg ctgataaatc tggagccggt gagcgtgggt
    12901 ctcgcggtat cattgcagca ctggggccag atggtaagcc ctcccgtatc gtagttatct
    12961 acacgacggg gagtcaggca actatggatg aacgaaatag acagatcgct gagataggtg
    13021 cctcactgat taagcattgg taactgtcag accaagttta ctcatatata ctttagattg
    13081 atttaaaact tcatttttaa tttaaaagga tctaggtgaa gatccttttt gataatctca
    13141 tgaccaaaat cccttaacgt gagttttcgt tccactgagc gtcagacccc gtagaaaaga
    13201 tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa
    13261 aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga
    13321 aggtaactgg cttcagcaga gcgcagatac caaatactgt tcttctagtg tagccgtagt
    13381 taggccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt
    13441 taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat
    13501 agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct
    13561 tggagcgaac gacctacacc gaactgagat acctacagcg tgagctatga gaaagcgcca
    13621 cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag
    13681 agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc
    13741 gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga
    13801 aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca
    13861 tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgcc tttgagtgag
    13921 ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg
    13981 aagagcgccc aatacgcaaa ccgcctctcc ccgcgcgttg gccgattcat taatgcagct
    14041 ggcacgacag gtttcccgac tggaaagcgg gcagtgagcg caacgcaatt aatgtgagtt
    14101 agctcactca ttaggcaccc caggctttac actttatgct tccggctcgt atgttgtgtg
    14161 gaattgtgag cggataacaa tttcacacag gaaacagcta tgaccatgat tacgccaagc
    14221 gcgcaattaa ccctcactaa agggaacaaa agctggagct gcaagc
//
