LOCUS       sequence-463602-        1535 bp    DNA     linear   UNA 15-APR-2025
DEFINITION  Bacterial expression of human HOIL-1L NZF (195-252).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1535)
  AUTHORS   Michel MA, Scutts S, Komander D
  TITLE     Secondary interactions in ubiquitin-binding domains achieve linkage 
            or substrate specificity.
  JOURNAL   Cell Rep. 2024 Aug 27;43(8):114545. doi: 
            10.1016/j.celrep.2024.114545. Epub 2024 Jul 23.
  PUBMED    39052481
REFERENCE   2  (bases 1 to 1535)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell Rep.";
            date: "2024-08-27"; volume: "43(8):114545. doi"; pages: " 
            10.1016/j.celrep.2024.114545. Epub 2024 Jul 23"
COMMENT     Sequence Label: pOPINK-HOIL-1L NZF (195-252)
FEATURES             Location/Qualifiers
     source          1..1535
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(46..63)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             complement(261..284)
                     /codon_start=1
                     /product="recognition and cleavage site for human 
                     rhinovirus 3C and PreScission proteases"
                     /label=HRV 3C site
                     /translation="LEVLFQGP"
     CDS             complement(303..956)
                     /codon_start=1
                     /product="glutathione S-transferase from Schistosoma 
                     japonicum"
                     /label=GST
                     /translation="MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKK
                     FELGLEFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVLDIRY
                     GVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTHPDFMLYDALDVVL
                     YMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIAWPLQGWQATFGGGDHPPK"
     primer_bind     complement(323..345)
                     /label=pGEX 5'
                     /note="pGEX vectors, Glutathione-S-transferase, forward 
                     primer"
     CDS             complement(957..974)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     RBS             987..1009
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     primer_bind     complement(1048..1067)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(1049..1067)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 annnnactca gcttcctttc gggctttgtt agcagccgga tctcagtggt ggtggtggtg
       61 gtgtttaaat ggtctagaaa gctttactgc tggtactgac gcagcgcctc ctcctcgccc
      121 gccaggcgcg ctcgctcctc ctcgtcgggc tggtatgagg cggggacctg gtaggcctcg
      181 gggcgcgccc ggcagcacat ctcacagcca ggccgcgtgg gcttgttgat gaaggtgcac
      241 ccggggcact gccagcccac cgggccctga aacagaactt ccagaccgct gctcagatcc
      301 gattttggag gatggtcgcc accaccaaac gtggcttgcc agccctgcaa aggccatgct
      361 atatacttgc tggatttcaa gtacttatca atttgtggga tagcttcaat acgtttttta
      421 aaacaaacta attttgggaa cgcatccagg cacattgggt ccatgtataa aacaacatca
      481 agagcgtcat acaacatgaa gtcaggatgg gttacatgat caccatttaa atatgtttta
      541 tgacataaac gatcttcgaa cattttcagc atttcaggta gcttgctaag aaaatcaact
      601 ttgagagttt caaagtcttt actatatgca attctcgaaa caccgtatct aatatccaaa
      661 accgctcctt caagcattga aatctctgca cgctcttttg gacaaccacc caacatgttg
      721 tgcttgtcag ctatataacg tatgatggcc atagactgtg ttaatttaac atcaccatca
      781 atataataag gaagattggg aaactccaaa cccaattcaa actttttgtt tcgccattta
      841 tcaccttcat cgcgctcata caaatgctct tcatattttt cttcaagata ttccaaaaga
      901 agtcgagtgg gttgcacaag gcccttaatt ttccaataac ctagtatagg ggacatgtga
      961 tggtgatggt gatgtgccat ggtatatctc cttcttaaag ttaaacaaaa ttatttctag
     1021 aggggaattg gtatccgctc acaattcccc tatagtgagt cgtattaatt tcgcgggatc
     1081 gagatctcga tcctctacgc cggacgcatc gtgggccggn atcaccggcc ccnnaggtgc
     1141 ggttgctggc cccctaattc gccgaactcc cccgatgggg gaaaatcggg nncccccatt
     1201 tcgggntcat gaacgccttg tttccgcngg ggntaagggg ggcaggnccc cggggtccgg
     1261 gggaatnntt ggggcccctc tccttgnnng gcncnnnnnc ttggggnnnc agnnanncnn
     1321 nggnctnnnn cccaaaanca gggncggttt cctaaaggtn gantncnnnt aaaggtaaaa
     1381 tnncctnaaa tccccgagac ccctgctaaa ggggccnnan cctnnncccg ggagtagntn
     1441 nnaannccgn cnannnnnaa aganntncca ggtggnnntg antntttaca ccttgggcgg
     1501 tgctacattg tcnnnnacna aaattagtga tagna
//
