LOCUS       sequence-463607-        1277 bp    DNA     linear   UNA 15-APR-2025
DEFINITION  Bacterial expression of human HOIP NZF1 (351-379).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1277)
  AUTHORS   Michel MA, Scutts S, Komander D
  TITLE     Secondary interactions in ubiquitin-binding domains achieve linkage 
            or substrate specificity.
  JOURNAL   Cell Rep. 2024 Aug 27;43(8):114545. doi: 
            10.1016/j.celrep.2024.114545. Epub 2024 Jul 23.
  PUBMED    39052481
REFERENCE   2  (bases 1 to 1277)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell Rep.";
            date: "2024-08-27"; volume: "43(8):114545. doi"; pages: " 
            10.1016/j.celrep.2024.114545. Epub 2024 Jul 23"
COMMENT     Sequence Label: pOPINS-HOIP NZF1 (351-379)
FEATURES             Location/Qualifiers
     source          1..1277
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(47..64)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             complement(469..486)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     RBS             505..527
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     protein_bind    542..566
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     complement(566..585)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(567..585)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(669..688)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
ORIGIN
        1 agcccctggc cttccttttc ggggctttgt tagcagccgg atctcagtgg tggtggtggt
       61 ggtgtttaaa tggtctagaa agctttaggc cagccgagga cgctcacata tggaacatag
      121 cacagcagct gcctcattct caaaggtaca gctctggcag gcccaccgac cccgaccacc
      181 gatctgttcg cgatgcgctt caataatatc gttgtcttcc atatccaggt cttccggggt
      241 ctgatcggcc tgaatacgga tgccgtcata cagaaagcgc aggctatcca tttctttacc
      301 ctgacgtttc gcaaaggctt ccatcaggcg acgcagcggg gtggtttttt taattttaaa
      361 aaagatttcg ctgctgccgt cgctaacttt cagattaata tgggtttccg gtttcacttc
      421 cggtttaact tccggtttcg cttcctggtt cacttcgcta tcgctcccgt gatgatgatg
      481 gtgatggctg ctacccatgg tatatctcct tcttaaagtt aaacaaaatt atttctagag
      541 gggaattgtt atccgctcac aattccccta tagtgagtcg tattaatttc gcgggatcga
      601 gatctcgatc ctctacgccg gacgcatcgt ggccggcatc accggcgcca caggtgcggt
      661 tgctggcgcc tatatcgccg acatcaccga tggggaagat cgggctcgcc acttcgggct
      721 catgagcgct tgtttcggcg tgggtatggt ggcaggcccc gtggccgggg gactgttggg
      781 cgccatctcc ttgcatgcac cattccttgc ggcggcgggt gctcaacggc ctcaacctac
      841 tactgggctg cttcctaatg caggagtcgc ataaggggag agcgtcgaga tcccgggaca
      901 ccattcgaat ggcgcaaaac cttttcgcgg tatggcatga tagcgcccgg gaagagagtc
      961 aattcagggt ggtgaatgtg aaaccagtaa cgttatacga tgtcgcaagt atgccggttg
     1021 tctcttattc agaccgtttc ccgcgtgttg aatcagccag ccacgtttct gcgaaaacgc
     1081 gggaaaaatt gagcggcgat ggcggagctg aaatacattc ccaaacggcg tgcacaacaa
     1141 ctgtcgggca acagtcgatt gctgatggcg atggcacctc agtctggact gtacggcgcg
     1201 tcgcaatgtc cgggcattaa tctcggcgat acactgggat caagctgtgg tagtgtgcca
     1261 tatggatgaa tgataag
//
