LOCUS       sequence-46383-e         775 bp    DNA     linear   UNA 14-APR-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 775)
  AUTHORS   Lian X, Hsiao C, Wilson G, Zhu K, Hazeltine LB, Azarin SM, Raval KK,
            Zhang J, Kamp TJ, Palecek SP
  TITLE     Robust cardiomyocyte differentiation from human pluripotent stem 
            cells via temporal modulation of canonical Wnt signaling.
  JOURNAL   Proc Natl Acad Sci U S A. 2012 May 29.
  PUBMED    22645348
REFERENCE   2  (bases 1 to 775)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 14, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A. 2012 May 29."
COMMENT     Sequence Label: pXL004-ishRNA-scramble
FEATURES             Location/Qualifiers
     source          1..775
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(268..287)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
ORIGIN
        1 cggatgtacc ccgaggacgg cgccctgaag ggcgagatca agcagaggct gaagctgaag
       61 gacggcggcc actacgacgc tgaggtcaag accacctaca aggccaagaa gcccgtgcag
      121 ctgcccggcg cctacaacgt caacatcaag ttggacatca cctcccacaa cgaggactac
      181 accatcgtgg aacagtacga acgcgccgag ggccgccact ccaccggcgg catggacgag
      241 ctgtacaagg acccaccggt cgccaccatg accgagtaca agcccacggt gcgcctcgcc
      301 acccgcgacg acgtccccag ggccgtacgc accctcgccg ccgcgttcgc cgactacccc
      361 gccacgcgcc acaccgtcga tccggaccgc cacatcgagc gggtcaccga gctgcaagaa
      421 ctcttcctca cgcgcgtcgg gctcgacatc ggcaaggtgt gggtcgcgga cgacggcgcc
      481 gcggtggcgg tctggaccac gccggagagc gtcgaagcgg gggcggtgtt cgccgagatc
      541 ggcccgcgca tggccgagtt gagcggttcc cggctggccg cgcagcaaca gatggaaggc
      601 ctcctggcgc cgcaccggcc caaggagccc gcgtggttcc tggccaccgt cggcgtgtcg
      661 cccgaccacc agggcaaggg tctgggcagc gccgtcgtgc tccccggagt ggaggcggcc
      721 gagcgcgccg gggtgcccgc cttcctggag acctccgcgc cccgcaacct cccct
//
