LOCUS       sequence-464224-        5604 bp    DNA     circular SYN 28-AUG-2025
DEFINITION  transient expresses IRF1 in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5604)
  AUTHORS   Chou WC, Chen WT, Shen CY
  TITLE     A common variant in 11q23.3 associated with hyperlipidemia is 
            mediated by the binding and regulation of GATA4.
  JOURNAL   NPJ Genom Med. 2022 Jan 19;7(1):4. doi: 10.1038/s41525-021-00279-5.
  PUBMED    35046404
REFERENCE   2  (bases 1 to 5604)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Aug 28, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "NPJ Genom 
            Med."; date: "2022-01-19"; volume: "7(1):4. doi"; pages: " 
            10.1038/s41525-021-00279-5"
COMMENT     Sequence Label: pXJ-Myc-IRF1
FEATURES             Location/Qualifiers
     source          1..5604
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        65..368
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        369..572
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     523..543
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     intron          648..1220
                     /label=beta-globin intron
                     /note="intron from rabbit beta-globin gene"
     primer_bind     1228..1247
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     promoter        1275..1293
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             1314..1343
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     promoter        complement(2411..2429)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     polyA_signal    2703..2824
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(2740..2759)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     2794..2813
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(2852..2869)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(3023..3611)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(3103..3122)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(3782..4573,4574..4642))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     4405..4424
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(4643..4747)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     4815..4833
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(4871..4893)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     4993..5012
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     rep_origin      complement(5079..5534)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5216..5237)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     5428..5447
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
ORIGIN
        1 ctagagtccg ttacataacw tassgwamrk kgcgggcctc ttcgctatta cgccagctag
       61 agtccgttac ataacttacg gtaaatggcc cgcctggctg accgcccaac gacccccgcc
      121 cattgacgtc aataatgacg tatgttccca tagtaacgcc aatagggact ttccattgac
      181 gtcaatgggt ggagtattta cggtaaactg cccacttggc agtacatcaa gtgtatcata
      241 tgccaagtac gccccctatt gacgtcaatg acggtaaatg gcccgcctgg cattatgccc
      301 agtacatgac cttatgggac tttcctactt ggcagtacat ctacgtatta gtcatcgcta
      361 ttaccatggt gatgcggttt tggcagtaca tcaatgggcg tggatagcgg tttgactcac
      421 ggggatttcc aagtctccac cccattgacg tcaatgggag tttgttttgg caccaaaatc
      481 aacgggactt tccaaaatgt cgtaacaact ccgccccatt gacgcaaatg ggcggtaggc
      541 gtgtacggtg ggaggtctat ataagcagag ctcgtttagt gaacsgtcak wtsgmckggm
      601 smckmggctt ttgcaaaaag ctccggatcg atcctgagaa cttcagggtg agtttgggga
      661 cccttgattg ttctttcttt ttcgctattg taaaattcat gttatatgga gggggcaaag
      721 ttttcagggt gttgtttaga atgggaagat gtcccttgta tcaccatgga ccctcatgat
      781 aattttgttt ctttcacttt ctactctgtt gacaaccatt gtctcctctt attttctttt
      841 cattttctgt aactttttcg ttaaacttta gcttgcattt gtaacgaatt tttaaattca
      901 cttttgttta tttgtcagat tgtaagtact ttctctaatc actttttttt caaggcaatc
      961 agggtatatt atattgtact tcagcacagt tttagagaac aattgttata attaaatgat
     1021 aaggtagaat atttctgcat ataaattctg gctggcgtgg aaatattctt attggtagaa
     1081 acaactacat cctggtcatc atcctgcctt tctctttatg gttacaatga tatacactgt
     1141 ttgagatgag gataaaatac tctgagtcca aaccgggccc ctctgctaac catgttcatg
     1201 ccttcttctt tttcctacag ctcctgggca acgtgctggt tattgtgctg tctcatcatt
     1261 ttggcaaaga attgtaatac gactcactat agggcgaatt caccatggca gaggagcaaa
     1321 agctcatttc tgaagaggac ttgttgcgga aacatatggg atccatgccc atcactcgga
     1381 tgcgcatgag accctggcta gagatgcaga ttaattccaa ccaaatcccg gggctcatct
     1441 ggattaataa agaggagatg atcttccaga tcccatggaa gcatgctgcc aagcatggct
     1501 gggacatcaa caaggatgcc tgtttgttcc ggagctgggc cattcacaca ggccgataca
     1561 aagcagggga aaaggagcca gatcccaaga cgtggaaggc caactttcgc tgtgccatga
     1621 actccctgcc agatatcgag gaggtgaaag accagagcag gaacaagggc agctcagctg
     1681 tgcgagtgta ccggatgctt ccacctctca ccaagaacca gagaaaagaa agaaagtcga
     1741 agtccagccg agatgctaag agcaaggcca agaggaagtc atgtggggat tccagccctg
     1801 ataccttctc tgatggactc agcagctcca ctctgcctga tgaccacagc agctacacag
     1861 ttccaggcta catgcaggac ttggaggtgg agcaggccct gactccagca ctgtcgccat
     1921 gtgctgtcag cagcactctc cccgactggc acatcccagt ggaagttgtg ccggacagca
     1981 ccagtgatct gtacaacttc caggtgtcac ccatgccctc cacctctgaa gctacaacag
     2041 atgaggatga ggaagggaaa ttacctgagg acatcatgaa gctcttggag cagtcggagt
     2101 ggcagccaac aaacgtggat gggaaggggt acctactcaa tgaacctgga gtccagccca
     2161 cctctgtcta tggagacttt agctgtaagg aggagccaga aattgacagc ccaggggggg
     2221 atattgggct gagtctacag cgtgtcttca cagatctgaa gaacatggat gccacctggc
     2281 tggacagcct gctgacccca gtccggttgc cctccatcca ggccattccc tgtgcaccgt
     2341 agcagggccc ctcgaggggg ggcccggtac cttaattaat taaggtacca ggtaagtgta
     2401 cccaattcgc cctatagtga gtcgtattac aattcactcg atcgcccttc ccaacagttg
     2461 cgcagcctga atggcgaatg gagatccaat ttttaagtgt ataatgtgtt aaactactga
     2521 ttctaattgt ttgtgtattt tagattcaca gtcccaaggc tcatttcagg cccctcagtc
     2581 ctcacagtct gttcatgatc ataatcagcc ataccacatt tgtagaggtt ttacttgctt
     2641 taaaaaacct cccacacctc cccctgaacc tgaaacataa aatgaatgca attgttgttg
     2701 ttaacttgtt tattgcagct tataatggtt acaaataaag caatagcatc acaaatttca
     2761 caaataaagc atttttttca ctgcattcta gttgtggttt gtccaaactc atcaatgtat
     2821 cttaacgctc gactctagac tcttccgctt cctcgctcac tgactcgctg cgctcggtcg
     2881 ttcggctgcg gcgagcggta tcagctcact caaaggcggt aatacggtta tccacagaat
     2941 caggggataa cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta
     3001 aaaaggccgc gttgctggcg tttttccata ggctccgccc ccctgacgag catcacaaaa
     3061 atcgacgctc aagtcagagg tggcgaaacc cgacaggact ataaagatac caggcgtttc
     3121 cccctggaag ctccctcgtg cgctctcctg ttccgaccct gccgcttacc ggatacctgt
     3181 ccgcctttct cccttcggga agcgtggcgc tttctcatag ctcacgctgt aggtatctca
     3241 gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc gttcagcccg
     3301 accgctgcgc cttatccggt aactatcgtc ttgagtccaa cccggtaaga cacgacttat
     3361 cgccactggc agcagccact ggtaacagga ttagcagagc gaggtatgta ggcggtgcta
     3421 cagagttctt gaagtggtgg cctaactacg gctacactag aagaacagta tttggtatct
     3481 gcgctctgct gaagccagtt accttcggaa aaagagttgg tagctcttga tccggcaaac
     3541 aaaccaccgc tggtagcggt ggtttttttg tttgcaagca gcagattacg cgcagaaaaa
     3601 aaggatctca agaagatcct ttgatctttt ctacggggtc tgacgctcag tggaacgaaa
     3661 actcacgtta agggattttg gtcatgagat tatcaaaaag gatcttcacc tagatccttt
     3721 taaattaaaa atgaagtttt aaatcaatct aaagtatata tgagtaaact tggtctgaca
     3781 gttaccaatg cttaatcagt gaggcaccta tctcagcgat ctgtctattt cgttcatcca
     3841 tagttgcctg actccccgtc gtgtagataa ctacgatacg ggagggctta ccatctggcc
     3901 ccagtgctgc aatgataccg cgagacccac gctcaccggc tccagattta tcagcaataa
     3961 accagccagc cggaagggcc gagcgcagaa gtggtcctgc aactttatcc gcctccatcc
     4021 agtctattaa ttgttgccgg gaagctagag taagtagttc gccagttaat agtttgcgca
     4081 acgttgttgc cattgctaca ggcatcgtgg tgtcacgctc gtcgtttggt atggcttcat
     4141 tcagctccgg ttcccaacga tcaaggcgag ttacatgatc ccccatgttg tgcaaaaaag
     4201 cggttagctc cttcggtcct ccgatcgttg tcagaagtaa gttggccgca gtgttatcac
     4261 tcatggttat ggcagcactg cataattctc ttactgtcat gccatccgta agatgctttt
     4321 ctgtgactgg tgagtactca accaagtcat tctgagaata gtgtatgcgg cgaccgagtt
     4381 gctcttgccc ggcgtcaata cgggataata ccgcgccaca tagcagaact ttaaaagtgc
     4441 tcatcattgg aaaacgttct tcggggcgaa aactctcaag gatcttaccg ctgttgagat
     4501 ccagttcgat gtaacccact cgtgcaccca actgatcttc agcatctttt actttcacca
     4561 gcgtttctgg gtgagcaaaa acaggaaggc aaaatgccgc aaaaaaggga ataagggcga
     4621 cacggaaatg ttgaatactc atactcttcc tttttcaata ttattgaagc atttatcagg
     4681 gttattgtct catgagcgga tacatatttg aatgtattta gaaaaataaa caaatagggg
     4741 ttccgcgcac atttccccga aaagtgccac ctgacgtcta agaaaccatt attatcatga
     4801 cattaaccta taaaaatagg cgtatcacga ggccctttcg tctcgcgcgt ttcggtgatg
     4861 acggtgaaaa cctctgacac atgcagctcc cggagacggt cacagcttgt ctgtaagcgg
     4921 atgccgggag cagacaagcc cgtcagggcg cgtcagcggg tgttggcggg tgtcggggct
     4981 ggcttaacta tgcggcatca gagcagattg tactgagagt gcaccatatg cggtgtgaaa
     5041 taccgcacag atgcgtaagg agaaaatacc gcatcaggaa attgtaagcg ttaatatttt
     5101 gttaaaattc gcgttaaatt tttgttaaat cagctcattt tttaaccaat aggccgaaat
     5161 cggcaaaatc ccttataaat caaaagaata gaccgagata gggttgagtg ttgttccagt
     5221 ttggaacaag agtccactat taaagaacgt ggactccaac gtcaaagggc gaaaaaccgt
     5281 ctatcagggc gatggcccac tacgtgaacc atcaccctaa tcaagttttt tggggtcgag
     5341 gtgccgtaaa gcactaaatc ggaaccctaa agggagcccc cgatttagag cttgacgggg
     5401 aaagccggcg aacgtggcga gaaaggaagg gaagaaagcg aaaggagcgg gcgctagggc
     5461 gctggcaagt gtagcggtca cgctgcgcgt aaccaccaca cccgccgcgc ttaatgcgcc
     5521 gctacagggc gcgtccattc gccattcagg ctgcgcaact gttgggaagg gcgatcggtg
     5581 cgggcctctt cgctattacg ccag
//
