LOCUS       sequence-464228-        6070 bp    DNA     circular SYN 15-APR-2025
DEFINITION  transient expresses FOXA1 in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6070)
  AUTHORS   Chou WC, Chen WT, Shen CY
  TITLE     A common variant in 11q23.3 associated with hyperlipidemia is 
            mediated by the binding and regulation of GATA4.
  JOURNAL   NPJ Genom Med. 2022 Jan 19;7(1):4. doi: 10.1038/s41525-021-00279-5.
  PUBMED    35046404
REFERENCE   2  (bases 1 to 6070)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "NPJ Genom 
            Med."; date: "2022-01-19"; volume: "7(1):4. doi"; pages: " 
            10.1038/s41525-021-00279-5"
COMMENT     Sequence Label: pXJ-Myc-FOXA1
FEATURES             Location/Qualifiers
     source          1..6070
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(41..58)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(212..800)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(292..311)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(971..1762,1763..1831))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     1594..1613
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(1832..1936)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     2004..2022
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(2060..2082)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     2182..2201
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     rep_origin      complement(2268..2723)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(2405..2426)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     2617..2636
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     enhancer        2802..3105
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        3106..3309
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     3260..3280
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     3306..3330
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     intron          3471..4043
                     /label=beta-globin intron
                     /note="intron from rabbit beta-globin gene"
     primer_bind     4051..4070
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     promoter        4098..4116
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             4137..4166
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     promoter        complement(5670..5688)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     polyA_signal    join(5962..6070,1..13)
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5999..6018)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     join(6053..6070,1..2)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
ORIGIN
        1 tcaatgtatc ttaacgctcg actctagact cttccgcttc ctcgctcact gactcgctgc
       61 gctcggtcgt tcggctgcgg cgagcggtat cagctcactc aaaggcggta atacggttat
      121 ccacagaatc aggggataac gcaggaaaga acatgtgagc aaaaggccag caaaaggcca
      181 ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag gctccgcccc cctgacgagc
      241 atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc gacaggacta taaagatacc
      301 aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg
      361 gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc tcacgctgta
      421 ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg
      481 ttcagcccga ccgctgcgcc ttatccggta actatcgtct tgagtccaac ccggtaagac
      541 acgacttatc gccactggca gcagccactg gtaacaggat tagcagagcg aggtatgtag
      601 gcggtgctac agagttcttg aagtggtggc ctaactacgg ctacactaga agaacagtat
      661 ttggtatctg cgctctgctg aagccagtta ccttcggaaa aagagttggt agctcttgat
      721 ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag cagattacgc
      781 gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc tacggggtct gacgctcagt
      841 ggaacgaaaa ctcacgttaa gggattttgg tcatgagatt atcaaaaagg atcttcacct
      901 agatcctttt aaattaaaaa tgaagtttta aatcaatcta aagtatatat gagtaaactt
      961 ggtctgacag ttaccaatgc ttaatcagtg aggcacctat ctcagcgatc tgtctatttc
     1021 gttcatccat agttgcctga ctccccgtcg tgtagataac tacgatacgg gagggcttac
     1081 catctggccc cagtgctgca atgataccgc gagacccacg ctcaccggct ccagatttat
     1141 cagcaataaa ccagccagcc ggaagggccg agcgcagaag tggtcctgca actttatccg
     1201 cctccatcca gtctattaat tgttgccggg aagctagagt aagtagttcg ccagttaata
     1261 gtttgcgcaa cgttgttgcc attgctacag gcatcgtggt gtcacgctcg tcgtttggta
     1321 tggcttcatt cagctccggt tcccaacgat caaggcgagt tacatgatcc cccatgttgt
     1381 gcaaaaaagc ggttagctcc ttcggtcctc cgatcgttgt cagaagtaag ttggccgcag
     1441 tgttatcact catggttatg gcagcactgc ataattctct tactgtcatg ccatccgtaa
     1501 gatgcttttc tgtgactggt gagtactcaa ccaagtcatt ctgagaatag tgtatgcggc
     1561 gaccgagttg ctcttgcccg gcgtcaatac gggataatac cgcgccacat agcagaactt
     1621 taaaagtgct catcattgga aaacgttctt cggggcgaaa actctcaagg atcttaccgc
     1681 tgttgagatc cagttcgatg taacccactc gtgcacccaa ctgatcttca gcatctttta
     1741 ctttcaccag cgtttctggg tgagcaaaaa caggaaggca aaatgccgca aaaaagggaa
     1801 taagggcgac acggaaatgt tgaatactca tactcttcct ttttcaatat tattgaagca
     1861 tttatcaggg ttattgtctc atgagcggat acatatttga atgtatttag aaaaataaac
     1921 aaataggggt tccgcgcaca tttccccgaa aagtgccacc tgacgtctaa gaaaccatta
     1981 ttatcatgac attaacctat aaaaataggc gtatcacgag gccctttcgt ctcgcgcgtt
     2041 tcggtgatga cggtgaaaac ctctgacaca tgcagctccc ggagacggtc acagcttgtc
     2101 tgtaagcgga tgccgggagc agacaagccc gtcagggcgc gtcagcgggt gttggcgggt
     2161 gtcggggctg gcttaactat gcggcatcag agcagattgt actgagagtg caccatatgc
     2221 ggtgtgaaat accgcacaga tgcgtaagga gaaaataccg catcaggaaa ttgtaagcgt
     2281 taatattttg ttaaaattcg cgttaaattt ttgttaaatc agctcatttt ttaaccaata
     2341 ggccgaaatc ggcaaaatcc cttataaatc aaaagaatag accgagatag ggttgagtgt
     2401 tgttccagtt tggaacaaga gtccactatt aaagaacgtg gactccaacg tcaaagggcg
     2461 aaaaaccgtc tatcagggcg atggcccact acgtgaacca tcaccctaat caagtttttt
     2521 ggggtcgagg tgccgtaaag cactaaatcg gaaccctaaa gggagccccc gatttagagc
     2581 ttgacgggga aagccggcga acgtggcgag aaaggaaggg aagaaagcga aaggagcggg
     2641 cgctagggcg ctggcaagtg tagcggtcac gctgcgcgta accaccacac ccgccgcgct
     2701 taatgcgccg ctacagggcg cgtccattcg ccattcaggc tgcgcaactg ttgggaaggg
     2761 cgatcggtgc gggcctcttc gctattacgc cagctagagt ccgttacata acttacggta
     2821 aatggcccgc ctggctgacc gcccaacgac ccccgcccat tgacgtcaat aatgacgtat
     2881 gttcccatag taacgccaat agggactttc cattgacgtc aatgggtgga gtatttacgg
     2941 taaactgccc acttggcagt acatcaagtg tatcatatgc caagtacgcc ccctattgac
     3001 gtcaatgacg gtaaatggcc cgcctggcat tatgcccagt acatgacctt atgggacttt
     3061 cctacttggc agtacatcta cgtattagtc atcgctatta ccatggtgat gcggttttgg
     3121 cagtacatca atgggcgtgg atagcggttt gactcacggg gatttccaag tctccacccc
     3181 attgacgtca atgggagttt gttttggcac caaaatcaac gggactttcc aaaatgtcgt
     3241 aacaactccg ccccattgac gcaaatgggc ggtaggcgtg tacggtggga ggtctatata
     3301 agcagagctc gtttagtgaa ccgtcagatc gcctggagac gccatccacg ctgttttgac
     3361 ctccatagaa gacaccggga ccgatccagc ctccgcggcc gggaacggtg cattggaacg
     3421 gacccctagg cttttgcaaa aagctccgga tcgatcctga gaacttcagg gtgagtttgg
     3481 ggacccttga ttgttctttc tttttcgcta ttgtaaaatt catgttatat ggagggggca
     3541 aagttttcag ggtgttgttt agaatgggaa gatgtccctt gtatcaccat ggaccctcat
     3601 gataattttg tttctttcac tttctactct gttgacaacc attgtctcct cttattttct
     3661 tttcattttc tgtaactttt tcgttaaact ttagcttgca tttgtaacga atttttaaat
     3721 tcacttttgt ttatttgtca gattgtaagt actttctcta atcacttttt tttcaaggca
     3781 atcagggtat attatattgt acttcagcac agttttagag aacaattgtt ataattaaat
     3841 gataaggtag aatatttctg catataaatt ctggctggcg tggaaatatt cttattggta
     3901 gaaacaacta catcctggtc atcatcctgc ctttctcttt atggttacaa tgatatacac
     3961 tgtttgagat gaggataaaa tactctgagt ccaaaccggg cccctctgct aaccatgttc
     4021 atgccttctt ctttttccta cagctcctgg gcaacgtgct ggttattgtg ctgtctcatc
     4081 attttggcaa agaattgtaa tacgactcac tatagggcga attcaccatg gcagaggagc
     4141 aaaagctcat ttctgaagag gacttgttgc ggaaacatat gggatccagg atgttaggaa
     4201 ctgtgaagat ggaagggcat gaaaccagcg actggaacag ctactacgca gacacgcagg
     4261 aggcctactc ctccgtcccg gtcagcaaca tgaactcagg cctgggctcc atgaactcca
     4321 tgaacaccta catgaccatg aacaccatga ctacgagcgg caacatgacc ccggcgtcct
     4381 tcaacatgtc ctatgccaac ccgggcctag gggccggcct gagtcccggc gcagtagccg
     4441 gcatgccggg gggctcggcg ggcgccatga acagcatgac tgcggccggc gtgacggcca
     4501 tgggtacggc gctgagcccg agcggcatgg gcgccatggg tgcgcagcag gcggcctcca
     4561 tgaatggcct gggcccctac gcggccgcca tgaacccgtg catgagcccc atggcgtacg
     4621 cgccgtccaa cctgggccgc agccgcgcgg gcggcggcgg cgacgccaag acgttcaagc
     4681 gcagctaccc gcacgccaag ccgccctact cgtacatctc gctcatcacc atggccatcc
     4741 agcaggcgcc cagcaagatg ctcacgctga gcgagatcta ccagtggatc atggacctct
     4801 tcccctatta ccggcagaac cagcagcgct ggcagaactc catccgccac tcgctgtcct
     4861 tcaatgactg cttcgtcaag gtggcacgct ccccggacaa gccgggcaag ggctcctact
     4921 ggacgctgca cccggactcc ggcaacatgt tcgagaacgg ctgctacttg cgccgccaga
     4981 agcgcttcaa gtgcgagaag cagccggggg ccggcggcgg gggcgggagc ggaagcgggg
     5041 gcagcggcgc caagggcggc cctgagagcc gcaaggaccc ctctggcgcc tctaacccca
     5101 gcgccgactc gcccctccat cggggtgtgc acgggaagac cggccagcta gagggcgcgc
     5161 cggcccccgg gcccgccgcc agcccccaga ctctggacca cagtggggcg acggcgacag
     5221 ggggcgcctc ggagttgaag actccagcct cctcaactgc gccccccata agctccgggc
     5281 ccggggcgct ggcctctgtg cccgcctctc acccggcaca cggcttggca ccccacgagt
     5341 cccagctgca cctgaaaggg gacccccact actccttcaa ccacccgttc tccatcaaca
     5401 acctcatgtc ctcctcggag cagcagcata agctggactt caaggcatac gaacaggcac
     5461 tgcaatactc gccttacggc tctacgttgc ccgccagcct gcctctaggc agcgcctcgg
     5521 tgaccaccag gagccccatc gagccctcag ccctggagcc ggcgtactac caaggtgtgt
     5581 attccagacc cgtcctaaac acttcctagc tcgagggggg gcccggtacc ttaattaatt
     5641 aaggtaccag gtaagtgtac ccaattcgcc ctatagtgag tcgtattaca attcactcga
     5701 tcgcccttcc caacagttgc gcagcctgaa tggcgaatgg agatccaatt tttaagtgta
     5761 taatgtgtta aactactgat tctaattgtt tgtgtatttt agattcacag tcccaaggct
     5821 catttcaggc ccctcagtcc tcacagtctg ttcatgatca taatcagcca taccacattt
     5881 gtagaggttt tacttgcttt aaaaaacctc ccacacctcc ccctgaacct gaaacataaa
     5941 atgaatgcaa ttgttgttgt taacttgttt attgcagctt ataatggtta caaataaagc
     6001 aatagcatca caaatttcac aaataaagca tttttttcac tgcattctag ttgtggtttg
     6061 tccaaactca
//
