LOCUS       sequence-464306-       10478 bp    DNA     circular SYN 15-APR-2025
DEFINITION  Lentiviral EGFP lipid sensor for PI(3,5)P2.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10478)
  TITLE     expression plasmids
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 10478)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pLenti-EGFP-SnxA
FEATURES             Location/Qualifiers
     source          1..10478
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        1..380
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        382..580
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     535..555
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     LTR             598..778
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    825..950
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1443..1676
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1861..1905
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2203..2320
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        2367..3545
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          2598..3536
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     3493..3513
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     CDS             join(3566..3568,3569..3571,3572..4282)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(3566..3568,3569..3571,3572..4282)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(3611..3632)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(3872..3891)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     4219..4240
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     misc_feature    4934..5522
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(4987..5007)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(5405..5416)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             6046..6226
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(6252..6269)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    6258..6482
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      6528..6956
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6615..6634)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     6825..6846
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(6965..6985)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        6970..7299
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      7150..7285
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     7212..7231
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        7347..7394
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             7413..7787
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    7917..8038
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7954..7973)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     8008..8027
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(8087..8103)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8087..8103)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(8100..8122)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    8111..8127
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(8135..8141,8142..8159,8160..8165))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8180..8201
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(8318..8335)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(8489..9077)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8569..8588)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(9248..10039,10040..10108))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9871..9890
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(10109..10213)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(10288..10307)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
ORIGIN
        1 gacattgatt attgactagt tattaatagt aatcaattac ggggtcatta gttcatagcc
       61 catatatgga gttccgcgtt acataactta cggtaaatgg cccgcctggc tgaccgccca
      121 acgacccccg cccattgacg tcaataatga cgtatgttcc catagtaacg ccaataggga
      181 ctttccattg acgtcaatgg gtggagtatt tacggtaaac tgcccacttg gcagtacatc
      241 aagtgtatca tatgccaagt acgcccccta ttgacgtcaa tgacggtaaa tggcccgcct
      301 ggcattatgc ccagtacatg accttatggg actttcctac ttggcagtac atctacgtat
      361 tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg cgtggatagc
      421 ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg agtttgtttt
      481 ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca ttgacgcaaa
      541 tgggcggtag gcgtgtacgg tgggaggtct atataagcag cgcgttttgc ctgtactggg
      601 tctctctggt tagaccagat ctgagcctgg gagctctctg gctaactagg gaacccactg
      661 cttaagcctc aataaagctt gccttgagtg cttcaagtag tgtgtgcccg tctgttgtgt
      721 gactctggta actagagatc cctcagaccc ttttagtcag tgtggaaaat ctctagcagt
      781 ggcgcccgaa cagggacttg aaagcgaaag ggaaaccaga ggagctctct cgacgcagga
      841 ctcggcttgc tgaagcgcgc acggcaagag gcgaggggcg gcgactggtg agtacgccaa
      901 aaattttgac tagcggaggc tagaaggaga gagatgggtg cgagagcgtc agtattaagc
      961 gggggagaat tagatcgcga tgggaaaaaa ttcggttaag gccaggggga aagaaaaaat
     1021 ataaattaaa acatatagta tgggcaagca gggagctaga acgattcgca gttaatcctg
     1081 gcctgttaga aacatcagaa ggctgtagac aaatactggg acagctacaa ccatcccttc
     1141 agacaggatc agaagaactt agatcattat ataatacagt agcaaccctc tattgtgtgc
     1201 atcaaaggat agagataaaa gacaccaagg aagctttaga caagatagag gaagagcaaa
     1261 acaaaagtaa gaccaccgca cagcaagcgg ccgctgatct tcagacctgg aggaggagat
     1321 atgagggaca attggagaag tgaattatat aaatataaag tagtaaaaat tgaaccatta
     1381 ggagtagcac ccaccaaggc aaagagaaga gtggtgcaga gagaaaaaag agcagtggga
     1441 ataggagctt tgttccttgg gttcttggga gcagcaggaa gcactatggg cgcagcgtca
     1501 atgacgctga cggtacaggc cagacaatta ttgtctggta tagtgcagca gcagaacaat
     1561 ttgctgaggg ctattgaggc gcaacagcat ctgttgcaac tcacagtctg gggcatcaag
     1621 cagctccagg caagaatcct ggctgtggaa agatacctaa aggatcaaca gctcctgggg
     1681 atttggggtt gctctggaaa actcatttgc accactgctg tgccttggaa tgctagttgg
     1741 agtaataaat ctctggaaca gatttggaat cacacgacct ggatggagtg ggacagagaa
     1801 attaacaatt acacaagctt aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa
     1861 aagaatgaac aagaattatt ggaattagat aaatgggcaa gtttgtggaa ttggtttaac
     1921 ataacaaatt ggctgtggta tataaaatta ttcataatga tagtaggagg cttggtaggt
     1981 ttaagaatag tttttgctgt actttctata gtgaatagag ttaggcaggg atattcacca
     2041 ttatcgtttc agacccacct cccaaccccg aggggacccg acaggcccga aggaatagaa
     2101 gaagaaggtg gagagagaga cagagacaga tccattcgat tagtgaacgg atcggcactg
     2161 cgtgcgccaa ttctgcagac aaatggcagt attcatccac aattttaaaa gaaaaggggg
     2221 gattgggggg tacagtgcag gggaaagaat agtagacata atagcaacag acatacaaac
     2281 taaagaatta caaaaacaaa ttacaaaaat tcaaaatttt cgggtttatt acagggacag
     2341 cagagatcca gtttggttaa ttaattggct ccggtgcccg tcagtgggca gagcgcacat
     2401 cgcccacagt ccccgagaag ttgtggggag gggtcggcaa ttgaaccggt gcctagagaa
     2461 ggtggcgcgg ggtaaactgg gaaagtgatg tcgtgtactg gctccgcctt tttcccgagg
     2521 gtgggggaga accgtatata agtgcagtag tcgccgtgaa cgttcttttt cgcaacgggt
     2581 ttgccgccag aacacaggta agtgccgtgt gtggttcccg cgggcctggc ctctttacgg
     2641 gttatggccc ttgcgtgcct tgaattactt ccacctggct gcagtacgtg attcttgatc
     2701 ccgagcttcg ggttggaagt gggtgggaga gttcgaggcc ttgcgcttaa ggagcccctt
     2761 cgcctcgtgc ttgagttgag gcctggcctg ggcgctgggg ccgccgcgtg cgaatctggt
     2821 ggcaccttcg cgcctgtctc gctgctttcg ataagtctct agccatttaa aatttttgat
     2881 gacctgctgc gacgcttttt ttctggcaag atagtcttgt aaatgcgggc caagatctgc
     2941 acactggtat ttcggttttt ggggccgcgg gcggcgacgg ggcccgtgcg tcccagcgca
     3001 catgttcggc gaggcggggc ctgcgagcgc ggccaccgag aatcggacgg gggtagtctc
     3061 aagctggccg gcctgctctg gtgcctggcc tcgcgccgcc gtgtatcgcc ccgccctggg
     3121 cggcaaggct ggcccggtcg gcaccagttg cgtgagcgga aagatggccg cttcccggcc
     3181 ctgctgcagg gagctcaaaa tggaggacgc ggcgctcggg agagcgggcg ggtgagtcac
     3241 ccacacaaag gaaaagggcc tttccgtcct cagccgtcgc ttcatgtgac tccacggagt
     3301 accgggcgcc gtccaggcac ctcgattagt tctcgagctt ttggagtacg tcgtctttag
     3361 gttgggggga ggggttttat gcgatggagt ttccccacac tgagtgggtg gagactgaag
     3421 ttaggccagc ttggcacttg atgtaattct ccttggaatt tgcccttttt gagtttggat
     3481 cttggttcat tctcaagcct cagacagtgg ttcaaagttt ttttcttcca tttcaggtgt
     3541 cgtgaggaat tcggccatta cggccatggt gagcaagggc gaggagctgt tcaccggggt
     3601 ggtgcccatc ctggtcgagc tggacggcga cgtaaacggc cacaagttca gcgtgtccgg
     3661 cgagggcgag ggcgatgcca cctacggcaa gctgaccctg aagttcatct gcaccaccgg
     3721 caagctgccc gtgccctggc ccaccctcgt gaccaccctg acctacggcg tgcagtgctt
     3781 cagccgctac cccgaccaca tgaagcagca cgacttcttc aagtccgcca tgcccgaagg
     3841 ctacgtccag gagcgcacca tcttcttcaa ggacgacggc aactacaaga cccgcgccga
     3901 ggtgaagttc gagggcgaca ccctggtgaa ccgcatcgag ctgaagggca tcgacttcaa
     3961 ggaggacggc aacatcctgg ggcacaagct ggagtacaac tacaacagcc acaacgtcta
     4021 tatcatggcc gacaagcaga agaacggcat caaggtgaac ttcaagatcc gccacaacat
     4081 cgaggacggc agcgtgcagc tcgccgacca ctaccagcag aacaccccca tcggcgacgg
     4141 ccccgtgctg ctgcccgaca accactacct gagcacccag tccgccctga gcaaagaccc
     4201 caacgagaag cgcgatcaca tggtcctgct ggagttcgtg accgccgccg ggatcactct
     4261 cggcatggac gagctgtaca agtccggact cagatccatg gaagaactga acaagtacaa
     4321 gcgggacctc gagaacatca gcaccaagta catcctgctc gagaaagaga acaaagagct
     4381 gcgcgagaag aacgacgagc tgaaccggaa gctgaccatc cagctgaaca acaacgccgt
     4441 gctggaagag aagctgggcg tgcagaaccg gaacaacgag atctacatca ccgtgcctcg
     4501 gaagatccag ggcgaagagg gcctgatgag aaagtacacc gcctacgtga tcgaggtgga
     4561 aggcagcgag aacaagagat accaagtgac ccggcggtac aagcagttcg tgctgctgca
     4621 tacccagctc gtcagagtgt tcggcgagca cgatctgcct tctctgcctg ccaaagccaa
     4681 cggcctgtac ttcagcaagg acgaccacac cgagaagcgg agagtgaacc tgcaagagta
     4741 cctgcagaac ctggccaaga atcccgccat cctgaacagc cccgtgttct accacttcct
     4801 gaagagagat gagggccaga acatcgacca cgtgccaagc agcacaccca gccatcgaat
     4861 tctgcagtcg acggtaccgc gggcccggga tccaccggat ctagataact gaggccgcct
     4921 cggccgcggc cgcaatcaac ctctggatta caaaatttgt gaaagattga ctggtattct
     4981 taactatgtt gctcctttta cgctatgtgg atacgctgct ttaatgcctt tgtatcatgc
     5041 tattgcttcc cgtatggctt tcattttctc ctccttgtat aaatcctggt tgctgtctct
     5101 ttatgaggag ttgtggcccg ttgtcaggca acgtggcgtg gtgtgcactg tgtttgctga
     5161 cgcaaccccc actggttggg gcattgccac cacctgtcag ctcctttccg ggactttcgc
     5221 tttccccctc cctattgcca cggcggaact catcgccgcc tgccttgccc gctgctggac
     5281 aggggctcgg ctgttgggca ctgacaattc cgtggtgttg tcggggaaat catcgtcctt
     5341 tccttggctg ctcgcctgtg ttgccacctg gattctgcgc gggacgtcct tctgctacgt
     5401 cccttcggcc ctcaatccag cggaccttcc ttcccgcggc ctgctgccgg ctctgcggcc
     5461 tcttccgcgt cttcgccttc gccctcagac gagtcggatc tccctttggg ccgcctcccc
     5521 gcctgcctgc aggtttgtcg agacctagaa aaacatggag caatcacaag tagcaataca
     5581 gcagctacca atgctgattg tgcctggcta gaagcacaag aggaggagga ggtgggtttt
     5641 ccagtcacac ctcaggtacc tttaagacca atgacttaca aggcagctgt agatcttagc
     5701 cactttttaa aagaaaaggg gggactggaa gggctaattc actcccaacg aagacaagat
     5761 atccttgatc tgtggatcta ccacacacaa ggctacttcc ctgattggca gaactacaca
     5821 ccagggccag ggatcagata tccactgacc tttggatggt gctacaagct agtaccagtt
     5881 gagcaagaga aggtagaaga agccaatgaa ggagagaaca cccgcttgtt acaccctgtg
     5941 agcctgcatg ggatggatga cccggagaga gaagtattag agtggaggtt tgacagccgc
     6001 ctagcatttc atcacatggc ccgagagctg catccggact gtactgggtc tctctggtta
     6061 gaccagatct gagcctggga gctctctggc taactaggga acccactgct taagcctcaa
     6121 taaagcttgc cttgagtgct tcaagtagtg tgtgcccgtc tgttgtgtga ctctggtaac
     6181 tagagatccc tcagaccctt ttagtcagtg tggaaaatct ctagcagggc ccgtttaaac
     6241 ccgctgatca gcctcgactg tgccttctag ttgccagcca tctgttgttt gcccctcccc
     6301 cgtgccttcc ttgaccctgg aaggtgccac tcccactgtc ctttcctaat aaaatgagga
     6361 aattgcatcg cattgtctga gtaggtgtca ttctattctg gggggtgggg tggggcagga
     6421 cagcaagggg gaggattggg aagacaatag caggcatgct ggggatgcgg tgggctctat
     6481 ggcttctgag gcggaaagaa ccagctgggg ctctaggggg tatccccacg cgccctgtag
     6541 cggcgcatta agcgcggcgg gtgtggtggt tacgcgcagc gtgaccgcta cacttgccag
     6601 cgccctagcg cccgctcctt tcgctttctt cccttccttt ctcgccacgt tcgccggctt
     6661 tccccgtcaa gctctaaatc gggggctccc tttagggttc cgatttagtg ctttacggca
     6721 cctcgacccc aaaaaacttg attagggtga tggttcacgt agtgggccat cgccctgata
     6781 gacggttttt cgccctttga cgttggagtc cacgttcttt aatagtggac tcttgttcca
     6841 aactggaaca acactcaacc ctatctcggt ctattctttt gatttataag ggattttgcc
     6901 gatttcggcc tattggttaa aaaatgagct gatttaacaa aaatttaacg cgaattaatt
     6961 ctgtggaatg tgtgtcagtt agggtgtgga aagtccccag gctccccagc aggcagaagt
     7021 atgcaaagca tgcatctcaa ttagtcagca accaggtgtg gaaagtcccc aggctcccca
     7081 gcaggcagaa gtatgcaaag catgcatctc aattagtcag caaccatagt cccgccccta
     7141 actccgccca tcccgcccct aactccgccc agttccgccc attctccgcc ccatggctga
     7201 ctaatttttt ttatttatgc agaggccgag gccgcctctg cctctgagct attccagaag
     7261 tagtgaggag gcttttttgg aggcctaggc ttttgcaaaa agctcccggg agcttgtata
     7321 tccattttcg gatctgatca gcacgtgttg acaattaatc atcggcatag tatatcggca
     7381 tagtataata cgacaaggtg aggaactaaa ccatggccaa gttgaccagt gccgttccgg
     7441 tgctcaccgc gcgcgacgtc gccggagcgg tcgagttctg gaccgaccgg ctcgggttct
     7501 cccgggactt cgtggaggac gacttcgccg gtgtggtccg ggacgacgtg accctgttca
     7561 tcagcgcggt ccaggaccag gtggtgccgg acaacaccct ggcctgggtg tgggtgcgcg
     7621 gcctggacga gctgtacgcc gagtggtcgg aggtcgtgtc cacgaacttc cgggacgcct
     7681 ccgggccggc catgaccgag atcggcgagc agccgtgggg gcgggagttc gccctgcgcg
     7741 acccggccgg caactgcgtg cacttcgtgg ccgaggagca ggactgacac gtgctacgag
     7801 atttcgattc caccgccgcc ttctatgaaa ggttgggctt cggaatcgtt ttccgggacg
     7861 ccggctggat gatcctccag cgcggggatc tcatgctgga gttcttcgcc caccccaact
     7921 tgtttattgc agcttataat ggttacaaat aaagcaatag catcacaaat ttcacaaata
     7981 aagcattttt ttcactgcat tctagttgtg gtttgtccaa actcatcaat gtatcttatc
     8041 atgtctgtat accgtcgacc tctagctaga gcttggcgta atcatggtca tagctgtttc
     8101 ctgtgtgaaa ttgttatccg ctcacaattc cacacaacat acgagccgga agcataaagt
     8161 gtaaagcctg gggtgcctaa tgagtgagct aactcacatt aattgcgttg cgctcactgc
     8221 ccgctttcca gtcgggaaac ctgtcgtgcc agctgcatta atgaatcggc caacgcgcgg
     8281 ggagaggcgg tttgcgtatt gggcgctctt ccgcttcctc gctcactgac tcgctgcgct
     8341 cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa ggcggtaata cggttatcca
     8401 cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga
     8461 accgtaaaaa ggccgcgttg ctggcgtttt tccataggct ccgcccccct gacgagcatc
     8521 acaaaaatcg acgctcaagt cagaggtggc gaaacccgac aggactataa agataccagg
     8581 cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat
     8641 acctgtccgc ctttctccct tcgggaagcg tggcgctttc tcatagctca cgctgtaggt
     8701 atctcagttc ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa ccccccgttc
     8761 agcccgaccg ctgcgcctta tccggtaact atcgtcttga gtccaacccg gtaagacacg
     8821 acttatcgcc actggcagca gccactggta acaggattag cagagcgagg tatgtaggcg
     8881 gtgctacaga gttcttgaag tggtggccta actacggcta cactagaaga acagtatttg
     8941 gtatctgcgc tctgctgaag ccagttacct tcggaaaaag agttggtagc tcttgatccg
     9001 gcaaacaaac caccgctggt agcggtggtt tttttgtttg caagcagcag attacgcgca
     9061 gaaaaaaagg atctcaagaa gatcctttga tcttttctac ggggtctgac gctcagtgga
     9121 acgaaaactc acgttaaggg attttggtca tgagattatc aaaaaggatc ttcacctaga
     9181 tccttttaaa ttaaaaatga agttttaaat caatctaaag tatatatgag taaacttggt
     9241 ctgacagtta ccaatgctta atcagtgagg cacctatctc agcgatctgt ctatttcgtt
     9301 catccatagt tgcctgactc cccgtcgtgt agataactac gatacgggag ggcttaccat
     9361 ctggccccag tgctgcaatg ataccgcgag acccacgctc accggctcca gatttatcag
     9421 caataaacca gccagccgga agggccgagc gcagaagtgg tcctgcaact ttatccgcct
     9481 ccatccagtc tattaattgt tgccgggaag ctagagtaag tagttcgcca gttaatagtt
     9541 tgcgcaacgt tgttgccatt gctacaggca tcgtggtgtc acgctcgtcg tttggtatgg
     9601 cttcattcag ctccggttcc caacgatcaa ggcgagttac atgatccccc atgttgtgca
     9661 aaaaagcggt tagctccttc ggtcctccga tcgttgtcag aagtaagttg gccgcagtgt
     9721 tatcactcat ggttatggca gcactgcata attctcttac tgtcatgcca tccgtaagat
     9781 gcttttctgt gactggtgag tactcaacca agtcattctg agaatagtgt atgcggcgac
     9841 cgagttgctc ttgcccggcg tcaatacggg ataataccgc gccacatagc agaactttaa
     9901 aagtgctcat cattggaaaa cgttcttcgg ggcgaaaact ctcaaggatc ttaccgctgt
     9961 tgagatccag ttcgatgtaa cccactcgtg cacccaactg atcttcagca tcttttactt
    10021 tcaccagcgt ttctgggtga gcaaaaacag gaaggcaaaa tgccgcaaaa aagggaataa
    10081 gggcgacacg gaaatgttga atactcatac tcttcctttt tcaatattat tgaagcattt
    10141 atcagggtta ttgtctcatg agcggataca tatttgaatg tatttagaaa aataaacaaa
    10201 taggggttcc gcgcacattt ccccgaaaag tgccacctga cgtcgacgga tcgggagatc
    10261 tcccgatccc ctatggtgca ctctcagtac aatctgctct gatgccgcat agttaagcca
    10321 gtatctgctc cctgcttgtg tgttggaggt cgctgagtag tgcgcgagca aaatttaagc
    10381 tacaacaagg caaggcttga ccgacaattg catgaagaat ctgcttaggg ttaggcgttt
    10441 tgcgctgctt cgcgatgtac gggccagata tacgcgtt
//
