LOCUS       sequence-464352-        6836 bp    DNA     circular SYN 15-APR-2025
DEFINITION  Ralstonia pseudosolanacearum filamentous phage RSCq-based cloning 
            vector carrying spectinomycin resistance gene..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6836)
  TITLE     A filamentous phage-based versatile toolkit for molecular studies in
            Ralstonia pseudosolanacearum
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6836)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pRSCq2
FEATURES             Location/Qualifiers
     source          1..6836
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    501..519
                     /label=Tn5 ME
                     /note="hyperactive mosaic end for Tn5 transposase 
                     recognition (Reznikoff et al., 2004)"
     CDS             1637..2428
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
ORIGIN
        1 cgtttacccc tcgcccgggt taaccggcta tatttacgcc agaatcacgg gcgtaattca
       61 tcacggacat aatattaatt acatccttca tgccgtcaag caaagaggtg cttagatgag
      121 aattgagaaa tacttagatc aggcgatcga acgccacggc ctgaagaacg acagcaagct
      181 ggcagagatg ctaggtgtgg tgcaaagcgc ggtcagccac taccgcaccg gccgccgcac
      241 ggcggacaac gaagtgtgcc tccgcctggc gcagctgctc gagatggaga acccgctgcc
      301 gatcatcatg gcggccgaca tggaccgcgc cgaacgtgct ggccagcact ctctctggga
      361 agttttttcg acgaggatgg cagccagtaa cgcgacagcc gccctcctcc tggtactggt
      421 cgcgagcgca acaaattttg ttgcgccctc tcccgccaaa gccgcgccgt tgagccattc
      481 gacagctcaa cgattattgt ctgtctctta tacacatctc aaccatcatc gatgaattgc
      541 ttcgttaata cagatgtagg tgttccacag ggtagccagc agcatcctgc gatgcagatc
      601 cggatgccat ttcattacct ctttctccgc acccgacata gatccgaaga tcagcagttc
      661 aacctgttga tagtacgtac taagctctca tgtttcacgt actaagctct catgtttaac
      721 gtactaagct ctcatgttta acgaactaaa ccctcatggc taacgtacta agctctcatg
      781 gctaacgtac taagctctca tgtttcacgt actaagctct catgtttgaa caataaaatt
      841 aatataaatc agcaacttaa atagcctcta aggttttaag ttttataaga aaaaaaagaa
      901 tatataaggc ttttaaagct tttaaggttt aacggttgtg gacaacaagc cagggatctg
      961 ccatttcatt acctctttct ccgcacccga catagatccg gaacataatg gtgcagggcg
     1021 ctgacttccg cgtttccaga ctttacgaaa cacggaaacc gaagaccatt catgttgttg
     1081 ctcaggtcgc agacgttttg cagcagcagt cgcttcacgt tcgctcgcgt atcggtgatt
     1141 cattctgcta accagtaagg caaccccgcc agcctagccg ggtcctcaac gacaggagca
     1201 cgatcatgcg cacccgtggc caggacccaa cgctgcccga gatgcgccgc gtgcggctgc
     1261 tggagatggc ggacgcgatg gatatgttct gccaagggtt ggtttgcgca ttcacagggt
     1321 gtctcaaaat ctctgatgtt acattgcaca agataaaaat atatcatcat gaacaataaa
     1381 actgtctgct tacataaaca gtaatacaag gggtgttatg cgctcacgca actggtccag
     1441 aaccttgacc gaacgcagcg gtggtaacgg cgcagtggcg gttttcatgg cttgttatga
     1501 ctgttttttt ggggtacagt ctatgcctcg ggcatccaag cagcaagcgc gttacgccgt
     1561 gggtcgatgt ttgatgttat ggagcagcaa cgatgttacg cagcagggca gtcgccctaa
     1621 aacaaagtta aacatcatga gggaagcggt gatcgccgaa gtatcgactc aactatcaga
     1681 ggtagttggc gtcatcgagc gccatctcga accgacgttg ctggccgtac atttgtacgg
     1741 ctccgcagtg gatggcggcc tgaagccaca cagtgatatt gatttgctgg ttacggtgac
     1801 cgtaaggctt gatgaaacaa cgcggcgagc tttgatcaac gaccttttgg aaacttcggc
     1861 ttcccctgga gagagcgaga ttctccgcgc tgtagaagtc accattgttg tgcacgacga
     1921 catcattccg tggcgttatc cagctaagcg cgaactgcaa tttggagaat ggcagcgcaa
     1981 tgacattctt gcaggtatct tcgagccagc cacgatcgac attgatctgg ctatcttgct
     2041 gacaaaagca agagaacata gcgttgcctt ggtaggtcca gcggcggagg aactctttga
     2101 tccggttcct gaacaggatc tatttgaggc gctaaatgaa accttaacgc tatggaactc
     2161 gccgcccgac tgggctggcg atgagcgaaa tgtagtgctt acgttgtccc gcatttggta
     2221 cagcgcagta accggcaaaa tcgcgccgaa ggatgtcgct gccgactggg caatggagcg
     2281 cctgccggcc cagtatcagc ccgtcatact tgaagctaga caggcttatc ttggacaaga
     2341 agaagatcgc ttggcctcgc gcgcagatca gttggaagaa tttgtccact acgtgaaagg
     2401 cgagatcacc aaggtagtcg gcaaataatc agaattggtt aattggttgt aacactggca
     2461 gagcattacg ctgacttgac gggacggcgg ctttgttgaa taaatcgaac ttttgctgag
     2521 ttgaaggatc agatcacgca tcttcccgac aacgcagacc gttccgtggc aaagcaaaag
     2581 ttcaaaatca ccaactggtc cacctacaac aaagctctca tcaaccgtgg cggggatcct
     2641 ctagagtcga cctgcaggca tgcaagcttc agcgacgaat tcagtactca tatggagctc
     2701 ggtaccgacg acgagaccga cgacggtctc aacgcgctcc ctccaataac cggatcattt
     2761 tagacgtttc gcgttacgcc ttgcgcgtaa cgactttgcg atcaccataa attgcctcgc
     2821 cagccgcttg catctgcgcc tcactcacgg ccacagaacg gacaaacgaa gcgcgccaca
     2881 actcggactc ccgcaccttc tggcgctgtt cgaacgtatt cgccttcgca gccaacgcga
     2941 gcaagtccag cattgcgtcg aacgaaacgg gatttccgtt actcggcgta acggattttt
     3001 gctgcgactc aaacatcgca accttctctt ccaagacgcc aacataacgc aacgcttcct
     3061 gacgcccgtc ctcgcacgtc ataagctgcc ggcgcaacgc ctcaatctcc gcaagatttc
     3121 cgttactcag cgtaacggat ttggcggcat cgctgatctc ttccctcagc cgcctattct
     3181 cagcaaacgc cttattgcgt tcagcctccg ccagctccac ctggcgacgc atgctcacca
     3241 gatcagcgcg aaacttctcg acctccaggc ggcattcatc gtaagcatcc acgtcggaaa
     3301 gcatttccgt tacggtcata cgcttagtaa cggatttagc ctcaacaggc tgctgagcct
     3361 tacggcgtgc tcgatatgcg gcttgacgct ccgcgttggt catagcgtgc gccttacgcg
     3421 gacgaccgcg gccacgcctt tgctccatcc ccggcagatc gaccgtcaca ttatccgtta
     3481 cgtcacgcat attcggctcc cgatttgatg attccatttt acgttactgg taacggcaat
     3541 tcaattatcg ttacaattta gttcgtaacg gaaatttcgc gagcagcccc ggcgctagac
     3601 gccctcagcg ggctttggag ccgttttttc tgactgggcg gtcgcagggg tcagctgcgc
     3661 cgaaattggc tcctgcgccg cttgtggctc gcggtagggg tcaaacggcg gcctcctgat
     3721 ccactcccga cactgcatgt catcgaggcc ggcatccgtg ccttgagccg tgtagcacgt
     3781 gcacctggtc gacgtgcagg caccgccgat caccgtcggc atcgagcgaa tctggcgcaa
     3841 ctgcgcatag gccggcgccg tctcaggccg gccagagaca gcagggacga acgccgcgag
     3901 gatctccgcc ccgtccgtcg ccgcggcgga cggacccgcc ttgaccgcta ccgctagagc
     3961 tgaagccaca ccgcccctgc cctgctcggc cgcaacaggc ttagccggag tcgccgtcgt
     4021 caactggccg gtacggtaat agacccggta cgccaagaac gccgcgatca acacgcaggc
     4081 gatgaacagc atcagcaccg gcggtacggt gtacttgcgc ttgatgtgca gactggagga
     4141 cttgtacagg ccgaaactgg acttcggcag gctccacttc ttcttgatcg gcgcggtgtt
     4201 gaacgtctcc gggttcgcgc actccggcca ttcgtagtac cagcgtccga gcaagccagc
     4261 gtcccgcagg tggacatgct gccccaccag cttgcggata tgactgtcca agaacgtcgg
     4321 gttctgcgtg atcagaacaa acgtcacgcc ggtatgccgc accgtctcaa acgccgccac
     4381 gtggtcaggc accttggacc cggccgtgcg gacgcgaaac acccgctgcg cctcatccaa
     4441 cacgatcagc gagttcggcg ggaaggtgaa gtacggcagc atcatccctg ggttttcagg
     4501 gtcttcgcgc agctccgtcc agtccgaaac cgccggcgtc gggatatacg gcagcttcag
     4561 ctccgggatg cccatgacga agagcggacg cccctggtcg acggctgcct tcatcatctg
     4621 gaccgccaac gcggtcttcc cgccaccagg cgtggccgtg atcagcgtga tcggttgcgt
     4681 tgcgcgagga acctaatccg ttacgtcacg ctaggcagaa ccggcagcag gaatccggcc
     4741 cagagccaga aatcgatcgc gagcatcaca gccccttttt catcaccacc agcgcccagg
     4801 ccgccaccat cgcggcaacg acgccccacc ccatcgtcat gccatccttg aagctctctt
     4861 gcgggtcaca tgccggaaac gacagcgacg gcaacgacgc atcggtcaac gtgccgacgc
     4921 tcccatcact gcccacctga taccgccgca gcacccaccc gcccgtggtc ttgacgaact
     4981 cagacagata ggtgactgcc ccaggtgtct gcgacggagc gacggcgctg taataggcat
     5041 ccgtggccat gcccacatcg gcaaagcacc gtgcgcccac caacgcgccg tcagcagcca
     5101 atcggcaaag caccgtgcgc ggttttctcc ttgagatgga ggttgagaaa agctccgggc
     5161 cgttcaactc ccagagccag ggaacatcac gccttcttgg gctcagcctc agcacgctgc
     5221 agcggcttga tgctcgtaac gaccttctga ccgcccttat ccttgccgtt gctcgtctcg
     5281 accatcgaga cttccgcgat gaacgggaac gggttgtgga tgatcgcctt cacaaccgca
     5341 gacgactcgc acttcagctc ctgcgtgcag gtgcccttcg agtcttcgcc acgcagctcc
     5401 acatccgtgt agatcttccc ggtgtccagc tgcttgccat ccatgttgcc aacccacgtc
     5461 ttggcgcccc ggatggtcac gcgtgcaatc atttccatgg tttcactcct caggtttcgg
     5521 cactggatcg tgcgtgccgt acacgtgcct cgccagggcg gatttgtgca gctgagccgg
     5581 tacgccctga cggcgtatcg cgacaaccag cgcagcgatg tcttcctctg tgcagcgcag
     5641 ctcgtaatcg accgtagggc cgaactgcgt ctggatgtgc ttgagcttgc gttcgcggat
     5701 ggtttcgtcc tgaagctcca aggccttgac ctggtcagta ggaacgcgct gcggatccgc
     5761 agccatgaag gcctccaggg ccttgtacgc accagcgaaa tactggtcgc gcttgataag
     5821 gatttcgtgc ggaatcacgc gatccttggc gccgaactcg atctcgagac gcacccactc
     5881 gctatcctga ttgccgagct ggcggccctt ctcgtaagcc cgcagcatct tgccgttcgc
     5941 ccgacggcca atctcgaacg tcgtaccgcg acagcccttg ctgcccgcca cgccgctctc
     6001 gatcttgcga tacgtcggga tacgcccgcc cgcgttgaag tcgccggcgt agtacagctc
     6061 ttccatctgc gcgatgctca cctcgccctg gcagaagtcc atcgccaggt cgcaccgcgt
     6121 gatccgcgcg tcgaggtcct gcaccatcgc gtagacggct tgccagtcgc caatcgcggt
     6181 gcagccctgc cccggccagt ccaccaggat cgtgccgcca acgtgctcgc cgccgcaggc
     6241 aacgatcccg agcttcatcg tctcgccgtt gatgaacgcc agcaggtcgt agctgaactc
     6301 ataccggcgg aaccctttgc ccgcaggctt catcgtcacc ggcaccgaga acaccagctg
     6361 gaagtacctg cgcagctgct ccagggcgtc gctgatgctg ccgtcgggca ggaacgtgaa
     6421 cttgaaccag tccacgatcg cacctgcctt gcgttctgga ctttccccgg gttttaccgg
     6481 acccggggaa cggcctgtcg gccgcccagc ctcgctccgc tcggcccggt cggccgccat
     6541 gccgttctcg cgtgcgagcg ccggcccatg aactccatac gcaatgcctg tgggatcaag
     6601 atgacgagag ggggtgggac ggacgcgcat cagcgtgcgc cctcgcagcg ataccagttc
     6661 gccagatcgg cctggcgcga gttcttcagg aggtcgtcaa tgcgatgcat cgcaggacgc
     6721 gattgcaggc agcaggcgcc gcgcagaacg gcagccatca cgatggccag ctgcgcaccg
     6781 tgcgggtgaa gcacgtcgcc aatcaggtcc agctgcctat gatctctctg ctgcat
//
