LOCUS       sequence-464354-        6616 bp    DNA     circular SYN 15-APR-2025
DEFINITION  Ralstonia pseudosolanacearum filamentous phage RSCq-based cloning 
            vector carrying gentamicin resistance gene..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6616)
  TITLE     A filamentous phage-based versatile toolkit for molecular studies in
            Ralstonia pseudosolanacearum
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6616)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pRSCq4
FEATURES             Location/Qualifiers
     source          1..6616
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    501..519
                     /label=Tn5 ME
                     /note="hyperactive mosaic end for Tn5 transposase 
                     recognition (Reznikoff et al., 2004)"
     promoter        join(1458..1463,1464..1480,1481..1486)
                     /gene="intI1 (promoter lies within the coding sequence)"
                     /label=Pc promoter
                     /note="class 1 integron promoter"
     CDS             1675..2208
                     /codon_start=1
                     /gene="aacC1"
                     /product="gentamycin acetyltransferase"
                     /label=GmR
                     /note="confers resistance to gentamycin"
                     /translation="MLRSSNDVTQQGSRPKTKLGGSSMGIIRTCRLGPDQVKSMRAALD
                     LFGREFGDVATYSQHQPDSDYLGNLLRSKTFIALAAFDQEAVVGALAAYVLPRFEQPRS
                     EIYIYDLAVSGEHRRQGIATALINLLKHEANALGAYVIYVQADYGDDPAVALYTKLGIR
                     EEVMHFDIDPSTAT"
ORIGIN
        1 cgtttacccc tcgcccgggt taaccggcta tatttacgcc agaatcacgg gcgtaattca
       61 tcacggacat aatattaatt acatccttca tgccgtcaag caaagaggtg cttagatgag
      121 aattgagaaa tacttagatc aggcgatcga acgccacggc ctgaagaacg acagcaagct
      181 ggcagagatg ctaggtgtgg tgcaaagcgc ggtcagccac taccgcaccg gccgccgcac
      241 ggcggacaac gaagtgtgcc tccgcctggc gcagctgctc gagatggaga acccgctgcc
      301 gatcatcatg gcggccgaca tggaccgcgc cgaacgtgct ggccagcact ctctctggga
      361 agttttttcg acgaggatgg cagccagtaa cgcgacagcc gccctcctcc tggtactggt
      421 cgcgagcgca acaaattttg ttgcgccctc tcccgccaaa gccgcgccgt tgagccattc
      481 gacagctcaa cgattattgt ctgtctctta tacacatctc aaccatcatc gatgaattgc
      541 ttcgttaata cagatgtagg tgttccacag ggtagccagc agcatcctgc gatgcagatc
      601 cggatgccat ttcattacct ctttctccgc acccgacata gatccgaaga tcagcagttc
      661 aacctgttga tagtacgtac taagctctca tgtttcacgt actaagctct catgtttaac
      721 gtactaagct ctcatgttta acgaactaaa ccctcatggc taacgtacta agctctcatg
      781 gctaacgtac taagctctca tgtttcacgt actaagctct catgtttgaa caataaaatt
      841 aatataaatc agcaacttaa atagcctcta aggttttaag ttttataaga aaaaaaagaa
      901 tatataaggc ttttaaagct tttaaggttt aacggttgtg gacaacaagc cagggatctg
      961 ccatttcatt acctctttct ccgcacccga catagatccg gaacataatg gtgcagggcg
     1021 ctgacttccg cgtttccaga ctttacgaaa cacggaaacc gaagaccatt catgttgttg
     1081 ctcaggtcgc agacgttttg cagcagcagt cgcttcacgt tcgctcgcgt atcggtgatt
     1141 cattctgcta accagtaagg caaccccgcc agcctagccg ggtcctcaac gacaggagca
     1201 cgatcatgcg cacccgtggc caggacccaa cgctgcccga gatgcgccgc gtgcggctgc
     1261 tggagatggc ggacgcgatg gatatgttct gccaagggtt ggtttgcgca ttcacagggt
     1321 gtctcaaaat ctctgatgtt acattgcaca agataaaaat atatcatcat gaacaataaa
     1381 actgtctgct tacataaaca gtaatacaag gggtgttaag atcccctgat tccctttgtc
     1441 aacagcaatg gatcgaattg acataagcct gttcggttcg taaactgtaa tgcaagtagc
     1501 gtatgcgctc acgcaactgg tccagaacct tgaccgaacg cagcggtggt aacggcgcag
     1561 tggcggtttt catggcttgt tatgactgtt tttttgtaca gtctatgcct cgggcatcca
     1621 agcagcaagc gcgttacgcc gtgggtcgat gtttgatgtt atggagcagc aacgatgtta
     1681 cgcagcagca acgatgttac gcagcagggc agtcgcccta aaacaaagtt aggtggctca
     1741 agtatgggca tcattcgcac atgtaggctc ggccctgacc aagtcaaatc catgcgggct
     1801 gctcttgatc ttttcggtcg tgagttcgga gacgtagcca cctactccca acatcagccg
     1861 gactccgatt acctcgggaa cttgctccgt agtaagacat tcatcgcgct tgctgccttc
     1921 gaccaagaag cggttgttgg cgctctcgcg gcttacgttc tgcccaggtt tgagcagccg
     1981 cgtagtgaga tctatatcta tgatctcgca gtctccggcg agcaccggag gcagggcatt
     2041 gccaccgcgc tcatcaatct cctcaagcat gaggccaacg cgcttggtgc ttatgtgatc
     2101 tacgtgcaag cagattacgg tgacgatccc gcagtggctc tctatacaaa gttgggcata
     2161 cgggaagaag tgatgcactt tgatatcgac ccaagtaccg ccacctaatc agaattggtt
     2221 aattggttgt aacactggca gagcattacg ctgacttgac gggacggcgg ctttgttgaa
     2281 taaatcgaac ttttgctgag ttgaaggatc agatcacgca tcttcccgac aacgcagacc
     2341 gttccgtggc aaagcaaaag ttcaaaatca ccaactggtc cacctacaac aaagctctca
     2401 tcaaccgtgg cggggatcct ctagagtcga cctgcaggca tgcaagcttc agcgacgaat
     2461 tcagtactca tatggagctc ggtaccgacg acgagaccga cgacggtctc aacgcgctcc
     2521 ctccaataac cggatcattt tagacgtttc gcgttacgcc ttgcgcgtaa cgactttgcg
     2581 atcaccataa attgcctcgc cagccgcttg catctgcgcc tcactcacgg ccacagaacg
     2641 gacaaacgaa gcgcgccaca actcggactc ccgcaccttc tggcgctgtt cgaacgtatt
     2701 cgccttcgca gccaacgcga gcaagtccag cattgcgtcg aacgaaacgg gatttccgtt
     2761 actcggcgta acggattttt gctgcgactc aaacatcgca accttctctt ccaagacgcc
     2821 aacataacgc aacgcttcct gacgcccgtc ctcgcacgtc ataagctgcc ggcgcaacgc
     2881 ctcaatctcc gcaagatttc cgttactcag cgtaacggat ttggcggcat cgctgatctc
     2941 ttccctcagc cgcctattct cagcaaacgc cttattgcgt tcagcctccg ccagctccac
     3001 ctggcgacgc atgctcacca gatcagcgcg aaacttctcg acctccaggc ggcattcatc
     3061 gtaagcatcc acgtcggaaa gcatttccgt tacggtcata cgcttagtaa cggatttagc
     3121 ctcaacaggc tgctgagcct tacggcgtgc tcgatatgcg gcttgacgct ccgcgttggt
     3181 catagcgtgc gccttacgcg gacgaccgcg gccacgcctt tgctccatcc ccggcagatc
     3241 gaccgtcaca ttatccgtta cgtcacgcat attcggctcc cgatttgatg attccatttt
     3301 acgttactgg taacggcaat tcaattatcg ttacaattta gttcgtaacg gaaatttcgc
     3361 gagcagcccc ggcgctagac gccctcagcg ggctttggag ccgttttttc tgactgggcg
     3421 gtcgcagggg tcagctgcgc cgaaattggc tcctgcgccg cttgtggctc gcggtagggg
     3481 tcaaacggcg gcctcctgat ccactcccga cactgcatgt catcgaggcc ggcatccgtg
     3541 ccttgagccg tgtagcacgt gcacctggtc gacgtgcagg caccgccgat caccgtcggc
     3601 atcgagcgaa tctggcgcaa ctgcgcatag gccggcgccg tctcaggccg gccagagaca
     3661 gcagggacga acgccgcgag gatctccgcc ccgtccgtcg ccgcggcgga cggacccgcc
     3721 ttgaccgcta ccgctagagc tgaagccaca ccgcccctgc cctgctcggc cgcaacaggc
     3781 ttagccggag tcgccgtcgt caactggccg gtacggtaat agacccggta cgccaagaac
     3841 gccgcgatca acacgcaggc gatgaacagc atcagcaccg gcggtacggt gtacttgcgc
     3901 ttgatgtgca gactggagga cttgtacagg ccgaaactgg acttcggcag gctccacttc
     3961 ttcttgatcg gcgcggtgtt gaacgtctcc gggttcgcgc actccggcca ttcgtagtac
     4021 cagcgtccga gcaagccagc gtcccgcagg tggacatgct gccccaccag cttgcggata
     4081 tgactgtcca agaacgtcgg gttctgcgtg atcagaacaa acgtcacgcc ggtatgccgc
     4141 accgtctcaa acgccgccac gtggtcaggc accttggacc cggccgtgcg gacgcgaaac
     4201 acccgctgcg cctcatccaa cacgatcagc gagttcggcg ggaaggtgaa gtacggcagc
     4261 atcatccctg ggttttcagg gtcttcgcgc agctccgtcc agtccgaaac cgccggcgtc
     4321 gggatatacg gcagcttcag ctccgggatg cccatgacga agagcggacg cccctggtcg
     4381 acggctgcct tcatcatctg gaccgccaac gcggtcttcc cgccaccagg cgtggccgtg
     4441 atcagcgtga tcggttgcgt tgcgcgagga acctaatccg ttacgtcacg ctaggcagaa
     4501 ccggcagcag gaatccggcc cagagccaga aatcgatcgc gagcatcaca gccccttttt
     4561 catcaccacc agcgcccagg ccgccaccat cgcggcaacg acgccccacc ccatcgtcat
     4621 gccatccttg aagctctctt gcgggtcaca tgccggaaac gacagcgacg gcaacgacgc
     4681 atcggtcaac gtgccgacgc tcccatcact gcccacctga taccgccgca gcacccaccc
     4741 gcccgtggtc ttgacgaact cagacagata ggtgactgcc ccaggtgtct gcgacggagc
     4801 gacggcgctg taataggcat ccgtggccat gcccacatcg gcaaagcacc gtgcgcccac
     4861 caacgcgccg tcagcagcca atcggcaaag caccgtgcgc ggttttctcc ttgagatgga
     4921 ggttgagaaa agctccgggc cgttcaactc ccagagccag ggaacatcac gccttcttgg
     4981 gctcagcctc agcacgctgc agcggcttga tgctcgtaac gaccttctga ccgcccttat
     5041 ccttgccgtt gctcgtctcg accatcgaga cttccgcgat gaacgggaac gggttgtgga
     5101 tgatcgcctt cacaaccgca gacgactcgc acttcagctc ctgcgtgcag gtgcccttcg
     5161 agtcttcgcc acgcagctcc acatccgtgt agatcttccc ggtgtccagc tgcttgccat
     5221 ccatgttgcc aacccacgtc ttggcgcccc ggatggtcac gcgtgcaatc atttccatgg
     5281 tttcactcct caggtttcgg cactggatcg tgcgtgccgt acacgtgcct cgccagggcg
     5341 gatttgtgca gctgagccgg tacgccctga cggcgtatcg cgacaaccag cgcagcgatg
     5401 tcttcctctg tgcagcgcag ctcgtaatcg accgtagggc cgaactgcgt ctggatgtgc
     5461 ttgagcttgc gttcgcggat ggtttcgtcc tgaagctcca aggccttgac ctggtcagta
     5521 ggaacgcgct gcggatccgc agccatgaag gcctccaggg ccttgtacgc accagcgaaa
     5581 tactggtcgc gcttgataag gatttcgtgc ggaatcacgc gatccttggc gccgaactcg
     5641 atctcgagac gcacccactc gctatcctga ttgccgagct ggcggccctt ctcgtaagcc
     5701 cgcagcatct tgccgttcgc ccgacggcca atctcgaacg tcgtaccgcg acagcccttg
     5761 ctgcccgcca cgccgctctc gatcttgcga tacgtcggga tacgcccgcc cgcgttgaag
     5821 tcgccggcgt agtacagctc ttccatctgc gcgatgctca cctcgccctg gcagaagtcc
     5881 atcgccaggt cgcaccgcgt gatccgcgcg tcgaggtcct gcaccatcgc gtagacggct
     5941 tgccagtcgc caatcgcggt gcagccctgc cccggccagt ccaccaggat cgtgccgcca
     6001 acgtgctcgc cgccgcaggc aacgatcccg agcttcatcg tctcgccgtt gatgaacgcc
     6061 agcaggtcgt agctgaactc ataccggcgg aaccctttgc ccgcaggctt catcgtcacc
     6121 ggcaccgaga acaccagctg gaagtacctg cgcagctgct ccagggcgtc gctgatgctg
     6181 ccgtcgggca ggaacgtgaa cttgaaccag tccacgatcg cacctgcctt gcgttctgga
     6241 ctttccccgg gttttaccgg acccggggaa cggcctgtcg gccgcccagc ctcgctccgc
     6301 tcggcccggt cggccgccat gccgttctcg cgtgcgagcg ccggcccatg aactccatac
     6361 gcaatgcctg tgggatcaag atgacgagag ggggtgggac ggacgcgcat cagcgtgcgc
     6421 cctcgcagcg ataccagttc gccagatcgg cctggcgcga gttcttcagg aggtcgtcaa
     6481 tgcgatgcat cgcaggacgc gattgcaggc agcaggcgcc gcgcagaacg gcagccatca
     6541 cgatggccag ctgcgcaccg tgcgggtgaa gcacgtcgcc aatcaggtcc agctgcctat
     6601 gatctctctg ctgcat
//
