LOCUS       sequence-464355-       12747 bp    DNA     circular SYN 15-APR-2025
DEFINITION  LuxCDABE reporter vector for Ralstonia pseudosolanacearum 
            filamentous..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12747)
  TITLE     A filamentous phage-based versatile toolkit for molecular studies in
            Ralstonia pseudosolanacearum
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 12747)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pRSCqlux
FEATURES             Location/Qualifiers
     source          1..12747
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    501..519
                     /label=Tn5 ME
                     /note="hyperactive mosaic end for Tn5 transposase 
                     recognition (Reznikoff et al., 2004)"
     primer_bind     complement(1407..1426)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     CDS             1418..2233
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     CDS             1418..2233
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(1491..1510)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     CDS             2841..4283
                     /codon_start=1
                     /gene="luxC"
                     /product="LuxC fatty acid reductase"
                     /label=LuxC
                     /note="from the Photorhabdus luminescens bioluminescence 
                     operon"
                     /translation="MTKKISFIINGQVEIFPESDDLVQSINFGDNSVYLPILNDSHVKN
                     IIDCNGNNELRLHNIVNFLYTVGQRWKNEEYSRRRTYIRDLKKYMGYSEEMAKLEANWI
                     SMILCSKGGLYDVVENELGSRHIMDEWLPQDESYVRAFPKGKSVHLLAGNVPLSGIMSI
                     LRAILTKNQCIIKTSSTDPFTANALALSFIDVDPNHPITRSLSVIYWPHQGDTSLAKEI
                     MRHADVIVAWGGPDAINWAVEHAPSYADVIKFGSKKSLCIIDNPVDLTSAATGAAHDVC
                     FYDQRACFSAQNIYYMGNHYEEFKLALIEKLNLYAHILPNAKKDFDEKAAYSLVQKESL
                     FAGLKVEVDIHQRWMIIESNAGVEFNQPLGRCVYLHHVDNIEQILPYVQKNKTQTISIF
                     PWESSFKYRDALALKGAERIVEAGMNNIFRVGGSHDGMRPLQRLVTYISHERPSNYTAK
                     DVAVEIEQTRFLEEDKFLVFVP"
     CDS             4295..5218
                     /codon_start=1
                     /gene="luxD"
                     /product="LuxD acyltransferase"
                     /label=LuxD
                     /note="from the Photorhabdus luminescens bioluminescence 
                     operon"
                     /translation="MENESKYKTIDHVICVEGNKKIHVWETLPEENSPKRKNAIIIASG
                     FARRMDHFAGLAEYLSRNGFHVIRYDSLHHVGLSSGTIDEFTMSIGKQSLLAVVDWLTT
                     RKINNFGMLASSLSARIAYASLSEINASFLITAVGVVNLRYSLERALGFDYLSLPINEL
                     PDNLDFEGHKLGAEVFARDCLDFGWEDLASTINNMMYLDIPFIAFTANNDNWVKQDEVI
                     TLLSNIRSNRCKIYSLLGSSHDLSENLVVLRNFYQSVTKAAIAMDNDHLDIDVDITEPS
                     FEHLTIATVNERRMRIEIENQAISLS"
     CDS             5267..6349
                     /codon_start=1
                     /gene="luxA"
                     /product="LuxA luciferase subunit"
                     /label=LuxA
                     /note="from the Photorhabdus luminescens bioluminescence 
                     operon"
                     /translation="MKFGNFLLTYQPPQFSQTEVMKRLVKLGRISEECGFDTVWLLEHH
                     FTEFGLLGNPYVAAAYLLGATKKLNVGTAAIVLPTAHPVRQLEDVNLLDQMSKGRFRFG
                     ICRGLYNKDFRVFGTDMNNSRALAECWYGLIKNGMTEGYMEADNEHIKFHKVKVNPAAY
                     SRGGAPVYVVAESASTTEWAAQFGLPMILSWIINTNEKKAQLELYNEVAQEYGHDIHNI
                     DHCLSYITSVDHDSIKAKEICRKFLGHWYDSYVNATTIFDDSDQTRGYDFNKGQWRDFV
                     LKGHKDTNRRIDYSYEINPVGTPQECIDIIQKDIDATGISNICCGFEANGTVDEIIASM
                     KLFQSDVMPFLKEKQRSLLY"
     CDS             6368..7351
                     /codon_start=1
                     /gene="luxB"
                     /product="LuxB luciferase subunit"
                     /label=LuxB
                     /note="from the Photorhabdus luminescens bioluminescence 
                     operon"
                     /translation="MKFGLFFLNFINSTTVQEQSIVRMQEITEYVDKLNFEQILVYENH
                     FSDNGVVGAPLTVSGFLLGLTEKIKIGSLNHIITTHHPVAIAEEACLLDQLSEGRFILG
                     FSDCEKKDEMHFFNRPVEYQQQLFEECYEIINDALTTGYCNPDNDFYSFPKISVNPHAY
                     TPGGPRKYVTATSHHIVEWAAKKGIPLIFKWDDSNDVRYEYAERYKAVADKYDVDLSEI
                     DHQLMILVNYNEDSNKAKQETRAFISDYVLEMHPNENFENKLEEIIAENAVGNYTECIT
                     AAKLAIEKCGAKSVLLSFEPMNDLMSQKNVINIVDDNIKKYHMEYT"
     CDS             7530..8642
                     /codon_start=1
                     /gene="luxE"
                     /product="LuxE"
                     /label=LuxE
                     /note="from the Photorhabdus luminescens bioluminescence 
                     operon"
                     /translation="MTSYVDKQEITASSEIDDLIFSSDPLVWSYDEQEKIRKKLVLDAF
                     RNHYKHCREYRHYCQAHKVDDNITEIDDIPVFPTSVFKFTRLLTSQENEIESWFTSSGT
                     NGLKSQVARDRLSIERLLGSVSYGMKYVGSWFDHQIELVNLGPDRFNAHNIWFKYVMSL
                     VELLYPTTFTVTEERIDFVKTLNSLERIKNQGKDLCLIGSPYFIYLLCHYMKDKKISFS
                     GDKSLYIITGGGWKSYEKESLKRDDFNHLLFDTFNLSDISQIRDIFNQVELNTCFFEDE
                     MQRKHVPPWVYARALDPETLKPVPDGTPGLMSYMDASATSYPAFIVTDDVGIISREYGK
                     YPGVLVEILRRVNTRTQKGCALSLTEAFDS"
ORIGIN
        1 cgtttacccc tcgcccgggt taaccggcta tatttacgcc agaatcacgg gcgtaattca
       61 tcacggacat aatattaatt acatccttca tgccgtcaag caaagaggtg cttagatgag
      121 aattgagaaa tacttagatc aggcgatcga acgccacggc ctgaagaacg acagcaagct
      181 ggcagagatg ctaggtgtgg tgcaaagcgc ggtcagccac taccgcaccg gccgccgcac
      241 ggcggacaac gaagtgtgcc tccgcctggc gcagctgctc gagatggaga acccgctgcc
      301 gatcatcatg gcggccgaca tggaccgcgc cgaacgtgct ggccagcact ctctctggga
      361 agttttttcg acgaggatgg cagccagtaa cgcgacagcc gccctcctcc tggtactggt
      421 cgcgagcgca acaaattttg ttgcgccctc tcccgccaaa gccgcgccgt tgagccattc
      481 gacagctcaa cgattattgt ctgtctctta tacacatctc aaccatcatc gatgaattgc
      541 ttcgttaata cagatgtagg tgttccacag ggtagccagc agcatcctgc gatgcagatc
      601 cggatgccat ttcattacct ctttctccgc acccgacata gatccgaaga tcagcagttc
      661 aacctgttga tagtacgtac taagctctca tgtttcacgt actaagctct catgtttaac
      721 gtactaagct ctcatgttta acgaactaaa ccctcatggc taacgtacta agctctcatg
      781 gctaacgtac taagctctca tgtttcacgt actaagctct catgtttgaa caataaaatt
      841 aatataaatc agcaacttaa atagcctcta aggttttaag ttttataaga aaaaaaagaa
      901 tatataaggc ttttaaagct tttaaggttt aacggttgtg gacaacaagc cagggatctg
      961 ccatttcatt acctctttct ccgcacccga catagatccg gaacataatg gtgcagggcg
     1021 ctgacttccg cgtttccaga ctttacgaaa cacggaaacc gaagaccatt catgttgttg
     1081 ctcaggtcgc agacgttttg cagcagcagt cgcttcacgt tcgctcgcgt atcggtgatt
     1141 cattctgcta accagtaagg caaccccgcc agcctagccg ggtcctcaac gacaggagca
     1201 cgatcatgcg cacccgtggc caggacccaa cgctgcccga gatgcgccgc gtgcggctgc
     1261 tggagatggc ggacgcgatg gatatgttct gccaagggtt ggtttgcgca ttcacagggt
     1321 gtctcaaaat ctctgatgtt acattgcaca agataaaaat atatcatcat gaacaataaa
     1381 actgtctgct tacataaaca gtaatacaag gggtgttatg agccatattc aacgggaaac
     1441 gtcttgctcg aggccgcgat taaattccaa catggatgct gatttatatg ggtataaatg
     1501 ggctcgcgat aatgtcgggc aatcaggtgc gacaatctat cgattgtatg ggaagcccga
     1561 tgcgccagag ttgtttctga aacatggcaa aggtagcgtt gccaatgatg ttacagatga
     1621 gatggtcaga ctaaactggc tgacggaatt tatgcctctt ccgaccatca agcattttat
     1681 ccgtactcct gatgatgcat ggttactcac cactgcgatc cccggaaaaa cagcattcca
     1741 ggtattagaa gaatatcctg attcaggtga aaatattgtt gatgcgctgg cagtgttcct
     1801 gcgccggttg cattcgattc ctgtttgtaa ttgtcctttt aacagcgatc gcgtatttcg
     1861 tctcgctcag gcgcaatcac gaatgaataa cggtttggtt gatgcgagtg attttgatga
     1921 cgagcgtaat ggctggcctg ttgaacaagt ctggaaagaa atgcataaac ttttgccatt
     1981 ctcaccggat tcagtcgtca ctcatggtga tttctcactt gataacctta tttttgacga
     2041 ggggaaatta ataggttgta ttgatgttgg acgagtcgga atcgcagacc gataccagga
     2101 tcttgccatc ctatggaact gcctcggtga gttttctcct tcattacaga aacggctttt
     2161 tcaaaaatat ggtattgata atcctgatat gaataaattg cagtttcatt tgatgctcga
     2221 tgagtttttc taatcagaat tggttaattg gttgtaacac tggcagagca ttacgctgac
     2281 ttgacgggac ggcggctttg ttgaataaat cgaacttttg ctgagttgaa ggatcagatc
     2341 acgcatcttc ccgacaacgc agaccgttcc gtggcaaagc aaaagttcaa aatcaccaac
     2401 tggtccacct acaacaaagc tctcatcaac cgtggcgggg atcctctaga gtcgacctgc
     2461 aggcatgcaa gcttcagcca gaattctgcc gcaagcactc agggcgcaag ggctgctaaa
     2521 ggaagcggaa cacgtagaaa gccagtccgc agaaacggtg ctgaccccgg atgaatgtca
     2581 gctactgggc tatctggaca agggaaaacg caagcgcaaa gagaaagcag gtagcttgca
     2641 gtgggcttac atggcgatag ctagactggg cggttttatg gacagcaagc gaaccggaat
     2701 tgccagctgg ggcgccctct ggtaaggttg ggaagccctg caaagtaaac tggatggctt
     2761 tcttgccgcc aaggatctga tggcgcaggg gatcaagatc tgatcaagag acaggatgag
     2821 gatcgtttcg catgggtacc atgactaaaa aaatttcatt cattattaac ggccaggttg
     2881 aaatctttcc cgaaagtgat gatttagtgc aatccattaa ttttggtgat aatagtgttt
     2941 acctgccaat attgaatgac tctcatgtaa aaaacattat tgattgtaat ggaaataacg
     3001 aattacggtt gcataacatt gtcaattttc tctatacggt agggcaaaga tggaaaaatg
     3061 aagaatactc aagacgcagg acatacattc gtgacttaaa aaaatatatg ggatattcag
     3121 aagaaatggc taagctagag gccaattgga tatctatgat tttatgttct aaaggcggcc
     3181 tttatgatgt tgtagaaaat gaacttggtt ctcgccatat catggatgaa tggctacctc
     3241 aggatgaaag ttatgttcgg gcttttccga aaggtaaatc tgtacatctg ttggcaggta
     3301 atgttccatt atctgggatc atgtctatat tacgcgcaat tttaactaag aatcagtgta
     3361 ttataaaaac atcgtcaacc gatcctttta ccgctaatgc attagcgtta agttttattg
     3421 atgtagaccc taatcatccg ataacgcgct ctttatctgt tatatattgg ccccaccaag
     3481 gtgatacatc actcgcaaaa gaaattatgc gacatgcgga tgttattgtc gcttggggag
     3541 ggccagatgc gattaattgg gcggtagagc atgcgccatc ttatgctgat gtgattaaat
     3601 ttggttctaa aaagagtctt tgcattatcg ataatcctgt tgatttgacg tccgcagcga
     3661 caggtgcggc tcatgatgtt tgtttttacg atcagcgagc ttgtttttct gcccaaaaca
     3721 tatattacat gggaaatcat tatgaggaat ttaagttagc gttgatagaa aaacttaatc
     3781 tatatgcgca tatattaccg aatgccaaaa aagattttga tgaaaaggcg gcctattctt
     3841 tagttcaaaa agaaagcttg tttgctggat taaaagtaga ggtggatatt catcaacgtt
     3901 ggatgattat tgagtcaaat gcaggtgtgg aatttaatca accacttggc agatgtgtgt
     3961 accttcatca cgtcgataat attgagcaaa tattgcctta tgttcaaaaa aataagacgc
     4021 aaaccatatc tatttttcct tgggagtcat catttaaata tcgagatgcg ttagcattaa
     4081 aaggtgcgga aaggattgta gaagcaggaa tgaataacat atttcgagtt ggtggatctc
     4141 atgacggaat gcgaccgttg caacgattag tgacatatat ttctcatgaa aggccatcta
     4201 actatacggc taaggatgtt gcggttgaaa tagaacagac tcgattcctg gaagaagata
     4261 agttccttgt atttgtccca taataggtaa aagtatggaa aatgaatcaa aatataaaac
     4321 catcgaccac gttatttgtg ttgaaggaaa taaaaaaatt catgtttggg aaacgctgcc
     4381 agaagaaaac agcccaaaga gaaagaatgc cattattatt gcgtctggtt ttgcccgcag
     4441 gatggatcat tttgctggtc tggcggaata tttatcgcgg aatggatttc atgtgatccg
     4501 ctatgattcg cttcaccacg ttggattgag ttcagggaca attgatgaat ttacaatgtc
     4561 tataggaaag cagagcttgt tagcagtggt tgattggtta actacacgaa aaataaataa
     4621 cttcggtatg ttggcttcaa gcttatctgc gcggatagct tatgcaagcc tatctgaaat
     4681 caatgcttcg tttttaatca ccgcagtcgg tgttgttaac ttaagatatt ctcttgaaag
     4741 agctttaggg tttgattatc tcagtctacc cattaatgaa ttgccggata atctagattt
     4801 tgaaggccat aaattgggtg ctgaagtctt tgcgagagat tgtcttgatt ttggttggga
     4861 agatttagct tctacaatta ataacatgat gtatcttgat ataccgttta ttgcttttac
     4921 tgcaaataac gataattggg tcaagcaaga tgaagttatc acattgttat caaatattcg
     4981 tagtaatcga tgcaagatat attctttgtt aggaagttcg catgacttga gtgaaaattt
     5041 agtggtcctg cgcaattttt atcaatcggt tacgaaagcc gctatcgcga tggataatga
     5101 tcatctggat attgatgttg atattactga accgtcattt gaacatttaa ctattgcgac
     5161 agtcaatgaa cgccgaatga gaattgagat tgaaaatcaa gcaatttctc tgtcttaaaa
     5221 tctattgaga tattctatca ctcaaatagc aatataagga ctctctatga aatttggaaa
     5281 ctttttgctt acataccaac ctccccaatt ttctcaaaca gaggtaatga aacgtttggt
     5341 taaattaggt cgcatctctg aggagtgtgg ttttgatacc gtatggttac tggagcatca
     5401 tttcacggag tttggtttgc ttggtaaccc ttatgtcgct gctgcatatt tacttggcgc
     5461 gactaaaaaa ttgaatgtag gaactgccgc tattgttctt cccacagccc atccagtacg
     5521 ccaacttgaa gatgtgaatt tattggatca aatgtcaaaa ggacgatttc ggtttggtat
     5581 ttgccgaggg ctttacaaca aggactttcg cgtattcggc acagatatga ataacagtcg
     5641 cgccttagcg gaatgctggt acgggctgat aaagaatggc atgacagagg gatatatgga
     5701 agctgataat gaacatatca agttccataa ggtaaaagta aaccccgcgg cgtatagcag
     5761 aggtggcgca ccggtttatg tggtggctga atcagcttcg acgactgagt gggctgctca
     5821 atttggccta ccgatgatat taagttggat tataaatact aacgaaaaga aagcacaact
     5881 tgagctttat aatgaagtgg ctcaagaata tgggcacgat attcataata tcgaccattg
     5941 cttatcatat ataacatctg tagatcatga ctcaattaaa gcgaaagaga tttgccggaa
     6001 atttctgggg cattggtatg attcttatgt gaatgctacg actatttttg atgattcaga
     6061 ccaaacaaga ggttatgatt tcaataaagg gcagtggcgt gactttgtat taaaaggaca
     6121 taaagatact aatcgccgta ttgattacag ttacgaaatc aatcccgtgg gaacgccgca
     6181 ggaatgtatt gacataattc aaaaagacat tgatgctaca ggaatatcaa atatttgttg
     6241 tggatttgaa gctaatggaa cagtagacga aattattgct tccatgaagc tcttccagtc
     6301 tgatgtcatg ccatttctta aagaaaaaca acgttcgcta ttatattaga aggctaagga
     6361 gaaagaaatg aaatttggat tgttcttcct taacttcatc aattcaacaa ctgttcaaga
     6421 acaaagtata gttcgcatgc aggaaataac ggagtatgtt gataagttga attttgaaca
     6481 gattttagtg tatgaaaatc atttttcaga taatggtgtt gtcggcgctc ctctgactgt
     6541 ttctggtttt ctgctcggtt taacagagaa aattaaaatt ggttcattaa atcacatcat
     6601 tacaactcat catcctgtcg ccatagcgga ggaagcttgc ttattggatc agttaagtga
     6661 agggagattt attttagggt ttagtgattg cgaaaaaaaa gatgaaatgc atttttttaa
     6721 tcgcccggtt gaatatcaac agcaactatt tgaagagtgt tatgaaatca ttaacgatgc
     6781 tttaacaaca ggctattgta atccagataa cgatttttat agcttcccta aaatatctgt
     6841 aaatccccat gcttatacgc caggcggacc tcggaaatat gtaacagcaa ccagtcatca
     6901 tattgttgag tgggcggcca aaaaaggtat tcctctcatc tttaagtggg atgattctaa
     6961 tgatgttaga tatgaatatg ctgaaagata taaagccgtt gcggataaat atgacgttga
     7021 cctatcagag atagaccatc agttaatgat attagttaac tataacgaag atagtaataa
     7081 agctaaacaa gagacgcgtg catttattag tgattatgtt cttgaaatgc accctaatga
     7141 aaatttcgaa aataaacttg aagaaataat tgcagaaaac gctgtcggaa attatacgga
     7201 gtgtataact gcggctaagt tggcaattga aaagtgtggt gcgaaaagtg tattgctgtc
     7261 ctttgaacca atgaatgatt tgatgagcca aaaaaatgta atcaatattg ttgatgataa
     7321 tattaagaag taccacatgg aatataccta atagatttcg agttgcagcg aggcggcaag
     7381 tgaacgaatc cccaggagca tagataacta tgtgactggg gtgagtgaaa gcagccaaca
     7441 aagcagcagc ttgaaagatg aagggtataa aagagtatga cagcagtgct gccatacttt
     7501 ctaatattat cttgaggagt aaaacaggta tgacttcata tgttgataaa caagaaatta
     7561 cagcaagctc agaaattgat gatttgattt tttcgagcga tccattagtg tggtcttacg
     7621 acgagcagga aaaaatcaga aagaaacttg tgcttgatgc atttcgtaat cattataaac
     7681 attgtcgaga atatcgtcac tactgtcagg cacacaaagt agatgacaat attacggaaa
     7741 ttgatgacat acctgtattc ccaacatcgg tttttaagtt tactcgctta ttaacttctc
     7801 aggaaaacga gattgaaagt tggtttacca gtagcggcac gaatggttta aaaagtcagg
     7861 tggcgcgtga cagattaagt attgagagac tcttaggctc tgtgagttat ggcatgaaat
     7921 atgttggtag ttggtttgat catcaaatag aattagtcaa tttgggacca gatagattta
     7981 atgctcataa tatttggttt aaatatgtta tgagtttggt ggaattgtta tatcctacga
     8041 catttaccgt aacagaagaa cgaatagatt ttgttaaaac attgaatagt cttgaacgaa
     8101 taaaaaatca agggaaagat ctttgtctta ttggttcgcc atactttatt tatttactct
     8161 gccattatat gaaagataaa aaaatctcat tttctggaga taaaagcctt tatatcataa
     8221 ccggaggcgg ctggaaaagt tacgaaaaag aatctctgaa acgtgatgat ttcaatcatc
     8281 ttttatttga tactttcaat ctcagtgata ttagtcagat ccgagatata tttaatcaag
     8341 ttgaactcaa cacttgtttc tttgaggatg aaatgcagcg taaacatgtt ccgccgtggg
     8401 tatatgcgcg agcgcttgat cctgaaacgt tgaaacctgt acctgatgga acgccggggt
     8461 tgatgagtta tatggatgcg tcagcaacca gttatccagc atttattgtt accgatgatg
     8521 tcgggataat tagcagagaa tatggtaagt atcccggcgt gctcgttgaa attttacgtc
     8581 gcgtcaatac gaggacgcag aaagggtgtg ctttaagctt aaccgaagcg tttgatagtt
     8641 gaacgcgctc cctccaataa ccggatcatt ttagacgttt cgcgttacgc cttgcgcgta
     8701 acgactttgc gatcaccata aattgcctcg ccagccgctt gcatctgcgc ctcactcacg
     8761 gccacagaac ggacaaacga agcgcgccac aactcggact cccgcacctt ctggcgctgt
     8821 tcgaacgtat tcgccttcgc agccaacgcg agcaagtcca gcattgcgtc gaacgaaacg
     8881 ggatttccgt tactcggcgt aacggatttt tgctgcgact caaacatcgc aaccttctct
     8941 tccaagacgc caacataacg caacgcttcc tgacgcccgt cctcgcacgt cataagctgc
     9001 cggcgcaacg cctcaatctc cgcaagattt ccgttactca gcgtaacgga tttggcggca
     9061 tcgctgatct cttccctcag ccgcctattc tcagcaaacg ccttattgcg ttcagcctcc
     9121 gccagctcca cctggcgacg catgctcacc agatcagcgc gaaacttctc gacctccagg
     9181 cggcattcat cgtaagcatc cacgtcggaa agcatttccg ttacggtcat acgcttagta
     9241 acggatttag cctcaacagg ctgctgagcc ttacggcgag ctcgatatgc ggcttgacgc
     9301 tccgcgttgg tcatagcgtg cgccttacgc ggacgaccgc ggccacgcct ttgctccatc
     9361 cccggcagat cgaccgtcac attatccgtt acgtcacgca tattcggctc ccgatttgat
     9421 gattccattt tacgttactg gtaacggcaa ttcaattatc gttacaattt agttcgtaac
     9481 ggaaatttcg cgagcagccc cggcgctaga cgccctcagc gggctttgga gccgtttttt
     9541 ctgactgggc ggtcgcaggg gtcagctgcg ccgaaattgg ctcctgcgcc gcttgtggct
     9601 cgcggtaggg gtcaaacggc ggcctcctga tccactcccg acactgcatg tcatcgaggc
     9661 cggcatccgt gccttgagcc gtgtagcacg tgcacctggt cgacgtgcag gcaccgccga
     9721 tcaccgtcgg catcgagcga atctggcgca actgcgcata ggccggcgcc gtctcaggcc
     9781 ggccagagac agcagggacg aacgccgcga ggatctccgc cccgtccgtc gccgcggcgg
     9841 acggacccgc cttgaccgct accgctagag ctgaagccac accgcccctg ccctgctcgg
     9901 ccgcaacagg cttagccgga gtcgccgtcg tcaactggcc ggtacggtaa tagacccggt
     9961 acgccaagaa cgccgcgatc aacacgcagg cgatgaacag catcagcacc ggcggtacgg
    10021 tgtacttgcg cttgatgtgc agactggagg acttgtacag gccgaaactg gacttcggca
    10081 ggctccactt cttcttgatc ggcgcggtgt tgaacgtctc cgggttcgcg cactccggcc
    10141 attcgtagta ccagcgtccg agcaagccag cgtcccgcag gtggacatgc tgccccacca
    10201 gcttgcggat atgactgtcc aagaacgtcg ggttctgcgt gatcagaaca aacgtcacgc
    10261 cggtatgccg caccgtctca aacgccgcca cgtggtcagg caccttggac ccggccgtgc
    10321 ggacgcgaaa cacccgctgc gcctcatcca acacgatcag cgagttcggc gggaaggtga
    10381 agtacggcag catcatccct gggttttcag ggtcttcgcg cagctccgtc cagtccgaaa
    10441 ccgccggcgt cgggatatac ggcagcttca gctccgggat gcccatgacg aagagcggac
    10501 gcccctggtc gacggctgcc ttcatcatct ggaccgccaa cgcggtcttc ccgccaccag
    10561 gcgtggccgt gatcagcgtg atcggttgcg ttgcgcgagg aacctaatcc gttacgtcac
    10621 gctaggcaga accggcagca ggaatccggc ccagagccag aaatcgatcg cgagcatcac
    10681 agcccctttt tcatcaccac cagcgcccag gccgccacca tcgcggcaac gacgccccac
    10741 cccatcgtca tgccatcctt gaagctctct tgcgggtcac atgccggaaa cgacagcgac
    10801 ggcaacgacg catcggtcaa cgtgccgacg ctcccatcac tgcccacctg ataccgccgc
    10861 agcacccacc cgcccgtggt cttgacgaac tcagacagat aggtgactgc cccaggtgtc
    10921 tgcgacggag cgacggcgct gtaataggca tccgtggcca tgcccacatc ggcaaagcac
    10981 cgtgcgccca ccaacgcgcc gtcagcagcc aatcggcaaa gcaccgtgcg cggttttctc
    11041 cttgagatgg aggttgagaa aagctccggg ccgttcaact cccagagcca gggaacatca
    11101 cgccttcttg ggctcagcct cagcacgctg cagcggcttg atgctcgtaa cgaccttctg
    11161 accgccctta tccttgccgt tgctcgtctc gaccatcgag acttccgcga tgaacgggaa
    11221 cgggttgtgg atgatcgcct tcacaaccgc agagctctcg cacttcagct cctgcgtgca
    11281 ggtgcccttc gagtcttcgc cacgcagctc cacatccgtg tagatcttcc cggtgtccag
    11341 ctgcttgcca tccatgttgc caacccacgt cttggcgccc cggatggtca cgcgtgcaat
    11401 catttccatg gtttcactcc tcaggtttcg gcactggatc gtgcgtgccg tacacgtgcc
    11461 tcgccagggc ggatttgtgc agctgagccg gtacgccctg acggcgtatc gcgacaacca
    11521 gcgcagcgat gtcttcctct gtgcagcgca gctcgtaatc gaccgtaggg ccgaactgcg
    11581 tctggatgtg cttgagcttg cgttcgcgga tggtttcgtc ctgaagctcc aaggccttga
    11641 cctggtcagt aggaacgcgc tgcggatccg cagccatgaa ggcctccagg gccttgtacg
    11701 caccagcgaa gtactggtcg cgcttgataa ggatttcgtg cggaatcacg cgatccttgg
    11761 cgccgaactc gatctcgaga cgcacccact cgctatcctg attgccgagc tggcggccct
    11821 tctcgtaagc ccgcagcatc ttgccgttcg cccgacggcc aatctcgaac gtcgtaccgc
    11881 gacagccctt gctgcccgcc acgccgctct cgatcttgcg atacgtcggg atacgcccgc
    11941 ccgcgttgaa gtcgccggcg tagtacagct cttccatctg cgcgatgctc acctcgccct
    12001 ggcagaagtc catcgccagg tcgcaccgcg tgatccgcgc gtcgaggtcc tgcaccatcg
    12061 cgtagacggc ttgccagtcg ccaatcgcgg tgcagccctg ccccggccag tccaccagga
    12121 tcgtgccgcc aacgtgctcg ccgccgcagg caacgatccc gagcttcatc gtctcgccgt
    12181 tgatgaacgc cagcaggtcg tagctgaact cataccggcg gaaccctttg cccgcaggct
    12241 tcatcgtcac cggcaccgag aacaccagct ggaagtacct gcgcagctgc tccagggcgt
    12301 cgctgatgct gccgtcgggc aggaacgtga acttgaacca gtccacgatc gcacctgcct
    12361 tgcgttctgg actttccccg ggttttaccg gacccgggga acggcctgtc ggccgcccag
    12421 cctcgctccg ctcggcccgg tcggccgcca tgccgttctc gcgtgcgagc gccggcccat
    12481 gaactccata cgcaatgcct gtgggatcaa gatgacgaga gggggtggga cggacgcgca
    12541 tcagcgtgcg ccctcgcagc gataccagtt cgccagatcg gcctggcgcg agttcttcag
    12601 gaggtcgtca atgcgatgca tcgcaggacg cgattgcagg cagcaggcgc cgcgcagaac
    12661 ggcagccatc acgatggcca gctgcgcacc gtgcgggtga agcacgtcgc caatcaggtc
    12721 cagctgccta tgatctctct gctgcat
//
