LOCUS       sequence-464371-       13318 bp    DNA     circular SYN 15-APR-2025
DEFINITION  GATEWAY compatible vector with a soybean Ubiquitin (GmUbi) promoter 
            driving in frame epitope fusion of 3xHA tag paired with ccdb 
            selection toxin and RUBY marker.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 13318)
  TITLE     GATEWAY compatible vector with a promoter driving in frame epitope 
            fusion paired with ccdb selection toxin and FP marker
REFERENCE   2  (bases 1 to 13318)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: Ubi:ccdb-3xha + RUBY
FEATURES             Location/Qualifiers
     source          1..13318
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     509..528
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     promoter        complement(join(928..933,934..950,951..956))
                     /label=tet promoter
                     /note="E. coli promoter for tetracycline efflux protein 
                     gene"
     rep_origin      1249..1443
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     CDS             complement(1509..2582)
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGASSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     CDS             complement(3011..3640)
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
     misc_feature    3904..3928
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     protein_bind    4962..5086
                     /gene="mutant version of attR"
                     /label=attR1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        join(5121..5126,5127..5144,5145..5151)
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an ""up"" mutation"
     CDS             5205..5864
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     complement(5271..5290)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     CDS             6206..6511
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /label=ccdB
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     primer_bind     6390..6409
                     /label=ccdB-fwd
                     /note="ccdB gene, forward primer"
     protein_bind    complement(6552..6676)
                     /gene="mutant version of attR"
                     /label=attR2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     CDS             6705..6794
                     /codon_start=1
                     /product="three tandem HA epitope tags"
                     /label=3xHA
                     /translation="YPYDVPDYAGYPYDVPDYAGSYPYDVPDYA"
     CDS             7609..11559
                     /label=RUBY
     CDS             9109..9165
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             10000..10056
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     terminator      11574..11826
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     misc_feature    11881..11905
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     rep_origin      complement(12028..12616)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(12108..12127)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(13008..13318,1..880))
                     /codon_start=1
                     /gene="tet"
                     /product="tetracycline efflux protein"
                     /label=TcR
                     /note="confers resistance to tetracycline"
                     /translation="MKSNNALIVILGTVTLDAVGIGLVMPVLPGLLRDIVHSDSIASHY
                     GVLLALYALMQFLCAPVLGALSDRFGRRPVLLASLLGATIDYAIMATTPVLWILYAGRI
                     VAGITGATGAVAGAYIADITDGEDRARHFGLMSACFGVGMVAGPVAGGLLGAISLHAPF
                     LAAAVLNGLNLLLGCFLMQESHKGERRPMPLRAFNPVSSFRWARGMTIVAALMTVFFIM
                     QLVGQVPAALWVIFGEDRFRWSATMIGLSLAVFGILHALAQAFVTGPATKRFGEKQAII
                     AGMAADALGYVLLAFATRGWMAFPIMILLASGGIGMPALQAMLSRQVDDDHQGQLQGSL
                     AALTSLTSIIGPLIVTAIYAASASTWNGLAWIVGAALYLVCLPALRRGAWSRATST"
ORIGIN
        1 gcaagacgta gcccagcgcg tcggccgcca tgccggcgat aatggcctgc ttctcgccga
       61 aacgtttggt ggcgggacca gtgacgaagg cttgagcgag ggcgtgcaag attccgaata
      121 ccgcaagcga caggccgatc atcgtcgcgc tccagcgaaa gcggtcctcg ccgaaaatga
      181 cccagagcgc tgccggcacc tgtcctacga gttgcatgat aaagaagaca gtcataagtg
      241 cggcgacgat agtcatgccc cgcgcccacc ggaaggagct gactgggttg aaggctctca
      301 agggcatcgg tcgacgctct cccttatgcg actcctgcat taggaagcag cccagtagta
      361 ggttgaggcc gttgagcacc gccgccgcaa ggaatggtgc atgcaaggag atggcgccca
      421 acagtccccc ggccacgggg cctgccacca tacccacgcc gaaacaagcg ctcatgagcc
      481 cgaagtggcg agcccgatct tccccatcgg tgatgtcggc gatataggcg ccagcaaccg
      541 cacctgtggc gccggtgatg ccggccacga tgcgtccggc gtagaggatc cacaggacgg
      601 gtgtggtcgc catgatcgcg tagtcgatag tggctccaag tagcgaagcg agcaggactg
      661 ggcggcggcc aaagcggtcg gacagtgctc cgagaacggg tgcgcataga aattgcatca
      721 acgcatatag cgctagcagc acgccatagt gactggcgat gctgtcggaa tggacgatat
      781 cccgcaagag gcccggcagt accggcataa ccaagcctat gcctacagca tccagggtga
      841 cggtgccgag gatgacgatg agcgcattgt tagatttcat acacggtgcc tgactgcgtt
      901 agcaatttaa ctgtgataaa ctaccgcatt aaagcttatc gatgataagc tgtcaaacat
      961 gagaattctt gaaaatattg cgctgccatt tttggggtga ggccgttcgc ggccgagggg
     1021 cgcagcccct ggggggatgg gagctcggtg ccttgatgtg ggcgccggcg gtcgagtggc
     1081 gacggcgcgg cttgtccgcg ccctggtaga ttgcctggcc gtaggccagc catttttgag
     1141 cggccagcgg ccgcgatagg ccgacgcgaa gcggcggggc gtagggagcg cagcgaccga
     1201 agggtaggcg ctttttgcag ctcttcggct gtgcgctggc cagacagtta tgcacaggcc
     1261 aggcgggttt taagagtttt aataagtttt aaagagtttt aggcggaaaa atcgcctttt
     1321 ttctctttta tatcagtcac ttacatgtgt gaccggttcc caatgtacgg ctttgggttc
     1381 ccaatgtacg ggttccggtt cccaatgtac ggctttgggt tcccaatgta cgtgctatcc
     1441 acaggaaaga agctttttcg acctttttcc cctgctaggg caatttgccc tagcatctgc
     1501 tccgtacatt aggaaccggc ggatgcttcg ccctcgatca ggttgcggta gcgcatgact
     1561 aggatcgggc cagcctgccc cgcctcctcc ttcaaatcgt actccggcag gtcatttgac
     1621 ccgatcagct tgcgcacggt gaaacagaac ttcttgaact ctccggcgct gccactgcgt
     1681 tcgtagatcg tcttgaacaa ccatctggct tctgccttgc ctgcggcgcg gcgtgccagg
     1741 cggtagagaa aacggccgat gccgggatcg atcaaaaagt aatcggggtg aaccgtcagc
     1801 acgtccgggt tcttgccttc tgtgatctcg cggtacatcc aatcagctag ctcgatctcg
     1861 atgtactccg gccgcccggt ttcgctcttt acgatcttgt agcggctaat caaggcttca
     1921 ccctcggata ccgtcaccag gcggccgttc ttggccttct tcgtacgctg catggcaacg
     1981 tgcgtggtgt ttaaccgaat gcaggtttct accaggtcgt ctttctgctt tccgccatcg
     2041 gctcgccggc agaacttgag tacgtccgca acgtgtggac ggaacacgcg gccgggcttg
     2101 tctcccttcc cttcccggta tcggttcatg gattcggtta gatgggaaac cgccatcagt
     2161 accaggtcgt aatcccacac actggccatg ccggccggcc ctgcggaaac ctctacgtgc
     2221 ccgtctggaa gctcgtagcg gatcacctcg ccagctcgtc ggtcacgctt cgacagacgg
     2281 aaaacggcca cgtccatgat gctgcgacta tcgcgggtgc ccacgtcata gagcatcgga
     2341 acgaaaaaat ctggttgctc gtcgcccttg ggcggcttcc taatcgacga cgcaccggct
     2401 gccggcggtt gccgggattc tttgcggatt cgatcagcgg ccgcttgcca cgattcaccg
     2461 gggcgtgctt ctgcctcgat gcgttgccgc tgggcggcct gcgcggcctt caacttctcc
     2521 accaggtcat cacccagcgc cgcgccgatt tgtaccgggc cggatggttt gcgaccgctc
     2581 acgccgattc ctcgggcttg ggggttccag tgccattgca gggccggcag acaacccagc
     2641 cgcttacgcc tggccaaccg cccgttcctc cacacatggg gcattccacg gcgtcggtgc
     2701 ctggttgttc ttgattttcc atgccgcctc ctttagccgc taaaattcat ctactcattt
     2761 attcatttgc tcatttactc tggtagctgc gcgatgtatt cagatagcag ctcggtaatg
     2821 gtcttgcctt ggcgtaccgc gtacatcttc agcttggtgt gatcctccgc cggcaactga
     2881 aagttgaccc gcttcatggc tggcgtgtct gccaggctgg ccaacgttgc agccttgctg
     2941 ctgcgtgcgc tcggacggcc ggcacttagc gtgtttgtgc ttttgctcat tttctcttta
     3001 cctcattaac tcaaatgagt tttgatttaa tttcagcggc cagcgcctgg acctcgcggg
     3061 cagcgtcgcc ctcgggttct gattcaagaa cggttgtgcc ggcggcggca gtgcctgggt
     3121 agctcacgcg ctgcgtgata cgggactcaa gaatgggcag ctcgtacccg gccagcgcct
     3181 cggcaacctc accgccgatg cgcgtgcctt tgatcgcccg cgacacgaca aaggccgctt
     3241 gtagccttcc atccgtgacc tcaatgcgct gcttaaccag ctccaccagg tcggcggtgg
     3301 cccatatgtc gtaagggctt ggctgcaccg gaatcagcac gaagtcggct gccttgatcg
     3361 cggacacagc caagtccgcc gcctggggcg ctccgtcgat cactacgaag tcgcgccggc
     3421 cgatggcctt cacgtcgcgg tcaatcgtcg ggcggtcgat gccgacaacg gttagcggtt
     3481 gatcttcccg cacggccgcc caatcgcggg cactgccctg gggatcggaa tcgactaaca
     3541 gaacatcggc cccggcgagt tgcagggcgc gggctagatg ggttgcgatg gtcgtcttgc
     3601 ctgacccgcc tttctggtta agtacagcga taaccttcat gcgttcccct tgcgtatttg
     3661 tttatttact catcgcatca tatacgcagc gaccgcatga cgcaagctgt tttactcaaa
     3721 tacacatcac ctttttagac ggcggcgctc ggtttcttca gcggccaagc tggccggcca
     3781 ggccgccagc ttggcatcag acaaaccggc caggatttca tgcagccgca cggctgatgg
     3841 gctgcctgta tcgagtggtg attttgtgcc gagctgccgg tcggggagct gttggctggc
     3901 tggtggcagg atatattgtg gtgtaaacaa attgacgctt agacaactta ataacacatt
     3961 gcggacgttt ttaatgtact ggggtggatg cagtgggccc cactctgtgg aagacaatgc
     4021 cccgcggtcc ggagtaacag ataaagcgaa gcttgaaggt gcatgtgact ccgtcaagat
     4081 tacgaaaccg ccaactacca cgcaaattgc aattctcaat ttcctagaag gactctccga
     4141 aaatgcatcc aataccaaat attacccgtg tcataggcac caagtgacac catacatgaa
     4201 cacgcgtcac aatatgactg gagaagggtt ccacacctta tgctataaaa cgccccacac
     4261 ccctcctcct tccttcgcag ttcaattcca atatattcca ttctctctgt gtatttccct
     4321 acctctccct tcaaggttag tcgatttctt ctgtttttct tcttcgttct ttccatgaat
     4381 tgtgtatgtt ctttgatcaa tacgatgttg atttgattgt gttttgtttg gtttcatcga
     4441 tcttcaattt tcataatcag attcagcttt tattatcttt acaacaacgt ccttaatttg
     4501 atgattcttt aatcgtagat ttgctctaat tagagctttt tcatgtcaga tccctttaca
     4561 acaagcctta attgttgatt cattaatcgt agattagggc ttttttcatt gattacttca
     4621 gatccgttaa acgtaaccat agatcagggc tttttcatga attacttcag atccgttaaa
     4681 caacagcctt attttttata cttctgtggt ttttcaagaa attgttcaga tccgttgaca
     4741 aaaagcctta ttcgttgatt ctatatcgtt tttcgagaga tattgctcag atctgttagc
     4801 aactgccttg tttgttgatt ctattgccgt ggattagggt tttttttcac gagattgctt
     4861 cagatccgta cttaagatta cgtaatggat tttgattctg atttatctgt gattgttgac
     4921 tcgacagcgg ccgcaccccc gggggcgcgc ccttaggtac cacaagtttg tacaaaaaag
     4981 ctgaacgaga aacgtaaaat gatataaata tcaatatatt aaattagatt ttgcataaaa
     5041 aacagactac ataatactgt aaaacacaac atatccagtc actatggcgg ccctcgaggg
     5101 ccgcattagg caccccaggc tttacacttt atgcttccgg ctcgtataat gtgtggattt
     5161 tgagttagga tccgtcgaga ttttcaggag ctaaggaagc taaaatggag aaaaaaatca
     5221 ctggatatac caccgttgat atatcccaat ggcatcgtaa agaacatttt gaggcatttc
     5281 agtcagttgc tcaatgtacc tataaccaga ccgttcagct ggatattacg gcctttttaa
     5341 agaccgtaaa gaaaaataag cacaagtttt atccggcctt tattcacatt cttgcccgcc
     5401 tgatgaatgc tcatccggaa ttccgtatgg caatgaaaga cggtgagctg gtgatatggg
     5461 atagtgttca cccttgttac accgttttcc atgagcaaac tgaaacgttt tcatcgctct
     5521 ggagtgaata ccacgacgat ttccggcagt ttctacacat atattcgcaa gatgtggcgt
     5581 gttacggtga aaacctggcc tatttcccta aagggtttat tgagaatatg tttttcgtct
     5641 cagccaatcc ctgggtgagt ttcaccagtt ttgatttaaa cgtggccaat atggacaact
     5701 tcttcgcccc cgttttcacc atgggcaaat attatacgca aggcgacaag gtgctgatgc
     5761 cgctggcgat tcaggttcat catgccgttt gtgatggctt ccatgtcggc agaatgctta
     5821 atgaattaca acagtactgc gatgagtggc agggcggggc gtaaagatct ggatccggct
     5881 tactaaaagc cagataacag tatgcgtatt tgcgcgctga tttttgcggt ataagaatat
     5941 atactgatat gtatacccga agtatgtcaa aaagaggtat gctatgaagc agcgtattac
     6001 agtgacagtt gacagcgaca gctatcagtt gctcaaggca tatatgatgt caatatctcc
     6061 ggtctggtaa gcacaaccat gcagaatgaa gcccgtcgtc tgcgtgccga acgctggaaa
     6121 gcggaaaatc aggaagggat ggctgaggtc gcccggttta ttgaaatgaa cggctctttt
     6181 gctgacgaga acaggggctg gtgaaatgca gtttaaggtt tacacctata aaagagagag
     6241 ccgttatcgt ctgtttgtgg atgtacagag tgatattatt gacacgcccg ggcgacggat
     6301 ggtgatcccc ctggccagtg cacgtctgct gtcagataaa gtctcccgtg aactttaccc
     6361 ggtggtgcat atcggggatg aaagctggcg catgatgacc accgatatgg ccagtgtgcc
     6421 ggtctccgtt atcggggaag aagtggctga tctcagccac cgcgaaaatg acatcaaaaa
     6481 cgccattaac ctgatgttct ggggaatata aatgtcaggc tcccttatac acagccagtc
     6541 tgcaggtcga ccatagtgac tggatatgtt gtgttttaca gtattatgta gtctgttttt
     6601 tatgcaaaat ctaatttaat atattgatat ttatatcatt ttacgtttct cgttcagctt
     6661 tcttgtacaa agtggttgat aacagcgggt taattaacat cttttaccca tacgatgttc
     6721 ctgactatgc gggctatccc tatgacgtcc cggactatgc aggatcctat ccatatgacg
     6781 ttccagatta cgctgctcag tgcagcgctt agcctaggtt cggttcgagt attatggcat
     6841 tgggaaaact gtttttcttg taccatttgt tgtgcttgta atttactgtg ttttttattc
     6901 ggttttcgct atcgaactgt gaaatggaaa tggatggaga agagttaatg aatgatatgg
     6961 tccttttgtt cattctcaaa ttaatattat ttgttttttc tcttatttgt tgtgtgttga
     7021 atttgaaatt ataagagata tgcaaacatt ttgttttgag taaaaatgtg tcaaatcaag
     7081 gttccagaag gtaattatcc aagatgtagc atcaagaatc caatgtttac gggaaaaact
     7141 atggaagtat tatgtgagct cagcaagaag cagatcaata tgcggcacat atgcaaccta
     7201 tgttcaaaaa tgaagaatgt acagatacaa gatcctatac tgccagaata cgaagaagaa
     7261 tacgtagaaa ttgaaaaaga agaaccaggc gaagaaaaga atcttgaaga cgtaagcact
     7321 gacgacaaca atgaaaagaa gaagataagg tcggtgattg tgaaagagac atagaggaca
     7381 catgtaaggt ggaaaatgta agggcggaaa gtaaccttat cacaaaggaa tcttatcccc
     7441 cactacttat ccttttatat ttttccgtgt catttttgcc cttgagtttt cctatataag
     7501 gaaccaagtt cggcatttgt gaaaacaaga aaaaatttgg tgtaagctat tttctttgaa
     7561 gtactgagga tacaacttca gagaaatttg taagtttgtg atccgcaaat ggatcatgcg
     7621 accctcgcca tgatcctcgc gatctggttc atcagcttcc acttcatcaa gctgctgttc
     7681 tcccagcaga ccaccaagct gcttccgcca ggaccaaagc cgcttccgat catcggcaac
     7741 atccttgagg tgggcaagaa gccgcatcgg tccttcgcca acctcgccaa gattcacggc
     7801 ccactcattt ccctcagact cggctctgtg accaccatcg ttgtgtcctc tgccgacgtg
     7861 gccaaagaga tgttcctcaa gaaggatcac ccgctctcca accgcacgat cccgaatagt
     7921 gttacagccg gcgaccacca caagctcacc atgtcttggc tcccggtgtc tccgaagtgg
     7981 cgcaacttcc gcaagattac cgccgtgcat ctgctctccc cacagagact cgatgcctgc
     8041 cagacattca ggcacgccaa ggtgcagcag ctctacgagt acgttcaaga gtgcgcccag
     8101 aaaggccagg ccgtggatat tggcaaggcc gcctttacga ccagcctcaa cctcctcagc
     8161 aagctgttct tcagcgtcga gctggcgcac cacaagtccc ataccagcca agagttcaaa
     8221 gagctgatct ggaacatcat ggaagatata ggcaagccga actacgccga ctacttcccg
     8281 attctcggct gcgttgaccc atctggcatt agaagaaggc tcgcctgctc cttcgacaag
     8341 ctgatcgccg tgttccaggg catcatctgc gagagactcg ccccagattc ctccaccaca
     8401 actaccacca ccaccgacga cgtgctcgat gtgctcctcc agctgttcaa gcagaacgag
     8461 ctgacgatgg gcgagatcaa ccacctcctc gtggacatct tcgacgccgg caccgatacc
     8521 acatcctcca cattcgagtg ggtgatgacc gagctgatcc gcaatccaga gatgatggaa
     8581 aaggcccaag aggaaatcaa gcaggtcctc ggcaaggaca agcagatcca agagtccgac
     8641 atcatcaacc tgccgtacct ccaggcgatc atcaaagaga cactccgcct ccatccgccg
     8701 accgtgttct tgctcccaag aaaggccgac accgatgtcg agctgtacgg ctacatcgtg
     8761 ccgaaggatg cccagatcct cgtgaacctc tgggccattg gcagggaccc aaacgcctgg
     8821 cagaacgccg atattttcag cccagagcgc ttcatcggct gcgagatcga tgttaagggc
     8881 cgcgatttcg gcctccttcc atttggcgct ggccgcagaa tttgcccagg catgaatctc
     8941 gccatcagga tgctcaccct catgctcgcc acactcctcc agttcttcaa ctggaagctc
     9001 gaaggcgaca tctccccgaa ggacctcgac atggacgaga agttcggcat tgcgctccaa
     9061 aagaccaagc cgctcaagct catcccgatt ccgcgctacg gtagcggagc taccaatttt
     9121 agcctcctta agcaggcagg tgatgtagaa gagaaccccg ggcctatgaa gatgatgaac
     9181 ggcgaggacg ccaacgacca gatgatcaaa gagtccttct tcatcaccca cggcaacccg
     9241 atcctcaccg tcgaggatac acatccgctc aggccgttct tcgagacatg gcgcgagaag
     9301 attttctcca agaagccgaa ggccatcctc atcatctccg gccactggga gacagtgaag
     9361 ccaaccgtga acgccgtgca catcaacgac accatccacg acttcgacga ctacccagcc
     9421 gccatgtacc agttcaagta cccagctcca ggcgagccag agcttgcgag aaaggtggaa
     9481 gagatcctca agaagtccgg gttcgagaca gccgagacag accaaaagag gggccttgat
     9541 cacggcgcct gggttccact catgctcatg tatccagagg cggacatccc ggtgtgccag
     9601 ctctcagttc agccacatct cgacggcacc taccactaca atctcggcag agccctcgcg
     9661 ccgctcaaga atgatggcgt gctcattatt ggctccggca gcgccacaca tccactcgat
     9721 gagacaccgc actacttcga tggtgttgcc ccttgggccg ctgccttcga ttcttggctt
     9781 aggaaggccc tcatcaacgg ccgcttcgag gaagtgaaca tctacgagag caaggccccg
     9841 aactggaagc tcgcccatcc atttccagag cacttctacc cgctccacgt tgtgctcggc
     9901 gctgctggtg aaaagtggaa ggccgagctg atccactcct cctgggatca tggcacactt
     9961 tgccacggct cctacaagtt cacctccgcc ggatccggag caaccaactt tagcctgctc
    10021 aagcaagcag gagatgttga ggaaaatcct ggccccatga ccgccatcaa gatgaacacc
    10081 aacggcgagg gcgagacaca gcacatcctc atgatcccgt tcatggcgca gggccacctc
    10141 aggccatttc tcgaactcgc catgttcctc tacaagcgct cccacgtgat catcaccctg
    10201 ctcacaactc cgctcaatgc cggcttcctc aggcacctcc ttcaccacca ttcctactcc
    10261 tccagcggca tcaggatcgt cgagctgcca ttcaactcca ccaaccacgg actcccaccg
    10321 ggcatcgaga acaccgataa gctcacactc ccgctcgtgg tgtccctctt ccattccacc
    10381 atcagcctcg atccgcacct ccgcgattac atctccaggc atttcagccc agccaggcca
    10441 ccactctgcg tgatccatga tgtgttcctc ggctgggttg accaggtggc caaggatgtg
    10501 ggctctacag gcgtggtgtt cacaacaggc ggcgcttatg gcacatccgc ctacgtgtcc
    10561 atctggaacg atctcccgca ccagaactac tccgacgacc aagagttccc gctgccaggc
    10621 ttcccagaga accataagtt ccgcaggtcc cagctccatc ggttcctcag atatgccgac
    10681 ggctccgacg attggtccaa gtatttccag ccgcagctcc gccagtccat gaagtctttt
    10741 ggctggctct gcaactccgt ggaagagatc gagacactcg gcttctccat cctccgcaac
    10801 tacaccaagc tgccgatctg gggcatcggc ccacttattg cttccccagt gcagcactcc
    10861 tcctccgaca acaattcaac aggcgccgag ttcgtgcagt ggctcagcct caaagagccg
    10921 gactccgtcc tctacatctc cttcggctcc cagaacacga tcagcccgac gcagatgatg
    10981 gaactcgctg ctggccttga gtcctccgag aagccattcc tctgggtgat cagagccccg
    11041 ttcggcttcg acatcaacga agagatgcgc ccagagtggc tgccagaggg ctttgaggaa
    11101 cgcatgaagg tgaagaaaca gggcaagctc gtgtacaagc tcggcccgca gcttgagatc
    11161 ctcaaccatg aatccatcgg cggctttctc acccactgcg gatggaacag catccttgag
    11221 tctcttcgcg agggcgttcc gatgcttgga tggccacttg ctgccgagca ggcctacaac
    11281 ctcaagtacc tcgaagatga gatgggcgtc gcggttgagc ttgctagagg cctcgaaggc
    11341 gagatctcca aagagaaggt caagcgcatc gtcgagatga tccttgagcg caacgagggc
    11401 tccaaaggct gggagatgaa gaatcgcgcc gtggaaatgg gcaaaaagct caaggacgcc
    11461 gtgaacgagg aaaaagagct gaagggctcc tccgtgaagg cgatcgacga tttcctcgac
    11521 gccgtcatgc aggccaaact tgagccaagc ctccagtgat agtgagatgt gcagatcgtt
    11581 caaacatttg gcaataaagt ttcttaagat tgaatcctgt tgccggtctt gcgatgatta
    11641 tcatataatt tctgttgaat tacgttaagc atgtaataat taacatgtaa tgcatgacgt
    11701 tatttatgag atgggttttt atgattagag tcccgcaatt atacatttaa tacgcgatag
    11761 aaaacaaaat atagcgcgca aactaggata aattatcgcg cgcggtgtca tctatgttac
    11821 tagatccgat gataagctgt caaacatgag ggacacgaag tggtttaaac tatcagtgtt
    11881 tgacaggata tattggcggg taaacctaag agaaaagagc gtttattaga ataatcggat
    11941 atttaaaagg gcgtgaaaag gtttatccgt tcgtccattt gtatgtgcca accacaggaa
    12001 ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc cgcccccctg acgagcatca
    12061 caaaaatcga cgctcaagtc agaggtggcg aaacccgaca ggactataaa gataccaggc
    12121 gtttccccct ggaagctccc tcgtgcgctc tcctgttccg accctgccgc ttaccggata
    12181 cctgtccgcc tttctccctt cgggaagcgt ggcgctttct catagctcac gctgtaggta
    12241 tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac cccccgttca
    12301 gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag tccaacccgg taagacacga
    12361 cttatcgcca ctggcagcag ccactggtaa caggattagc agagcgaggt atgtaggcgg
    12421 tgctacagag ttcttgaagt ggtggcctaa ctacggctac actagaagga cagtatttgg
    12481 tatctgcgct ctgctgaagc cagttacctt cggaaaaaga gttggtagct cttgatccgg
    12541 caaacaaacc accgctggta gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag
    12601 aaaaaaagga tctcaagaag atcctttgat cttttctacg gggtctgacg ctcagtggaa
    12661 cgaaaactca cgttaaggga ttttggtcat gagattatca aaaaggatct tcacctagat
    12721 ccttttaaat taaaaatgaa gttttaaatc aatctaaagt atatatgagt aaacttggtc
    12781 tgacagctaa aacaattcat ccagtaaaat ataatcgaat attatgcgca cccgtggcca
    12841 ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga
    12901 tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc
    12961 aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg
    13021 gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct
    13081 acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc
    13141 agcggtccaa tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc
    13201 tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg
    13261 ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgc gaacgcca
//
