LOCUS       sequence-464622-       12345 bp    DNA     circular SYN 15-APR-2025
DEFINITION  expresses LaccID on the surface of fly neurons.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12345)
  TITLE     Directed evolution of LaccID for cell surface proximity labeling and
            EM
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 12345)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: 10xUAS-wgSS-HA-V5-LaccID-CD2 (fly optimized, r...
FEATURES             Location/Qualifiers
     source          1..12345
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      1..457
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(88..107)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     298..319
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     583..605
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     597..614
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     598..614
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     624..643
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        624..642
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             complement(join(1447..2061,2132..2263,2467..2782,2844..3498,
                     3573..3846,4248..4319))
                     /codon_start=1
                     /gene="white"
                     /product="Drosophila white gene eye color pigment"
                     /label=mini-white
                     /note="This is a modified version of the white gene lacking
                     part of the first intron."
                     /translation="MGQEDQELLIRGGSKHPSAEHLNNGDSGAASQSCINQGFGQAKNY
                     GTLRPPSPPEDSGSGSGQLAENLTYAWHNMDIFGAVNQPGSGWRQLVNRTRGLFCNERH
                     IPAPRKHLLKNVCGVAYPGELLAVMGSSGAGKTTLLNALAFRSPQGIQVSPSGMRLLNG
                     QPVDAKEMQARCAYVQQDDLFIGSLTAREHLIFQAMVRMPRHLTYRQRVARVDQVIQEL
                     SLSKCQHTIIGVPGRVKGLSGGERKRLAFASEALTDPPLLICDEPTSGLDSFTAHSVVQ
                     VLKKLSQKGKTVILTIHQPSSELFELFDKILLMAEGRVAFLGTPSEAVDFFSYVGAQCP
                     TNYNPADFYVQVLAVVPGREIESRDRIAKICDNFAISKVARDMEQLLATKNLEKPLEQP
                     ENGYTYKATWFMQFRAVLWRSWLSVLKEPLLVKVRLIQTTMVAILIGLIFLGQQLTQVG
                     VMNINGAIFLFLTNMTFQNVFATINVFTSELPVFMREARSRLYRCDTYFLGKTIAELPL
                     FLTVPLVFTAIAYPMIGLRAGVLHFFNCLALVTLVANVSTSFGYLISCASSSTSMALSV
                     GPPVIIPFLLFGGFFLNSGSVPVYLKWLSYLSWFRYANEGLLINQWADVEPGEISCTSS
                     NTTCPSSGKVILETLNFSAADLPLDYVGLAILIVSFRVLAYLALRLRARRKE"
     primer_bind     4771..4789
                     /label=pCasper-F
                     /note="Drosophila mini-white gene, reverse primer"
     protein_bind    4912..4981
                     /label=attB
                     /bound_moiety="phage phi-C31 integrase"
                     /note="attB site for the phi-C31 integrase (Groth et al., 
                     2000)"
     protein_bind    5219..5649
                     /label=gypsy insulator
                     /bound_moiety="Su(Hw), CP190, andMod(mdg4)67.2"
                     /note="chromatin insulator from Drosophila"
     protein_bind    5667..5700
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     protein_bind    5713..5807
                     /label=5X UAS
                     /bound_moiety="GAL4"
                     /note="five tandem copies of the ""ScaI site"" 17-mer 
                     CGGAGTACTGTCCTCCG, an upstream activating sequence (UAS) 
                     that efficiently binds yeast Gal4 (Webster et al., 1988; 
                     Pfeiffer et al., 2010)"
     protein_bind    5823..5856
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     protein_bind    5869..5963
                     /label=5X UAS
                     /bound_moiety="GAL4"
                     /note="five tandem copies of the ""ScaI site"" 17-mer 
                     CGGAGTACTGTCCTCCG, an upstream activating sequence (UAS) 
                     that efficiently binds yeast Gal4 (Webster et al., 1988; 
                     Pfeiffer et al., 2010)"
     promoter        5987..6225
                     /label=hsp70 promoter
                     /note="Drosophila melanogaster hsp70Bb promoter"
     primer_bind     6172..6193
                     /label=pCasper-hs
                     /note="Drosophila Hsp70 promoter, forward primer"
     protein_bind    6271..6295
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             6430..6456
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             6484..6525
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     protein_bind    complement(8744..8768)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     intron          9075..9140
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             9270..9290
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    9562..9683
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(9599..9618)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     9653..9672
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     protein_bind    9705..10135
                     /label=gypsy insulator
                     /bound_moiety="Su(Hw), CP190, andMod(mdg4)67.2"
                     /note="chromatin insulator from Drosophila"
     primer_bind     complement(10154..10174)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(10154..10172)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(10193..10209)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(10193..10209)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(10206..10228)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    10217..10233
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(10241..10247,10248..10265,10266..10271))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    10286..10307
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(10424..10441)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(10595..11183)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(10675..10694)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(11354..12145,12146..12214))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     11977..11996
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(12215..12319)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 acgcgccctg tagcggcgca ttaagcgcgg cgggtgtggt ggttacgcgc agcgtgaccg
       61 ctacacttgc cagcgcccta gcgcccgctc ctttcgcttt cttcccttcc tttctcgcca
      121 cgttcgccgg ctttccccgt caagctctaa atcgggggct ccctttaggg ttccgattta
      181 gtgctttacg gcacctcgac cccaaaaaac ttgattaggg tgatggttca cgtagtgggc
      241 catcgccctg atagacggtt tttcgccctt tgacgttgga gtccacgttc tttaatagtg
      301 gactcttgtt ccaaactgga acaacactca accctatctc ggtctattct tttgatttat
      361 aagggatttt gccgatttcg gcctattggt taaaaaaatg agctgattta acaaaaattt
      421 aacgcgaatt ttaacaaaat attaacgctt acaatttcca ttcgccattc aggctgcgca
      481 actgttggga agggcgatcg gtgcgggcct cttcgctatt acgccagctg gcgaaagggg
      541 gatgtgctgc aaggcgatta agttgggtaa cgccagggtt ttcccagtca cgacgttgta
      601 aaacgacggc cagtgagcgc gcgtaatacg actcactata gggcgaattg ggtacaagct
      661 tcacgaccta ggcgcgccct agttccagtg aaatccaagc attttctaaa ttaaatgtat
      721 tcttattatt atagttgtta tttttgatat atataaacaa cactattatg cccaccattt
      781 ttttgagatg catctacaca aggaacaaac actggatgtc actttcagtt caaattgtaa
      841 cgctaatcac tccgaacagg tcacaaaaaa ttaccttaaa aagtcataat attaaattag
      901 aataaatata gctgtgaggg aaatatatac aaatatattg gagcaaataa attgtacata
      961 caaatattta ttactaattt ctattgagac gaaatgaacc actcggaacc atttgagcga
     1021 accgaatcgc gcggaactaa cgacagtcgc tccaaggtcg tcgaacaaaa ggtgaatgtg
     1081 ttgcggagag cgggtgggag acagcgaaag agcaactacg aaacgtggtg tggtggaggt
     1141 gaattatgaa gagggcgcgc gatttgaaaa gtatgtatat aaaaaatata tcccggtgtt
     1201 ttatgtagcg ataaacgagt ttttgatgta aggtatgcag gtgtgtaagt cttttggtta
     1261 gaagacaaat ccaaagtcta cttgtgggga tgttcgaagg ggaaatactt gtattctata
     1321 ggtcatatct tgtttttatt ggcacaaata taattacatt agctttttga gggggcaata
     1381 aacagtaaac acgatggtaa taatggtaaa aaaaaaaaac aagcagttat ttcggatata
     1441 tgtcggctac tccttgcgtc gggcccgaag tcttagagcc agatatgcga gcacccggaa
     1501 gctcacgatg agaatggcca gacccacgta gtccagcggc agatcggcgg cggagaagtt
     1561 aagcgtctcc aggatgacct tgcccgaact ggggcacgtg gtgttcgacg atgtgcagct
     1621 aatttcgccc ggctccacgt ccgcccattg gttaatcagc agaccctcgt tggcgtaacg
     1681 gaaccatgag aggtacgaca accatttgag gtatactggc accgagcccg agttcaagaa
     1741 gaagccgcca aagagcagga atggtatgat aaccggcgga cccacagaca gcgccatcga
     1801 ggtcgaggag ctggcgcagg atattagata tccgaaggac gttgacacat tggccaccag
     1861 agtgaccagc gccaggcagt tgaagaagtg cagcactccg gcccgcagtc cgatcatcgg
     1921 ataggcaatc gccgtgaaga ccagtggcac tgtgagaaaa agcggcaatt cggcaatcgt
     1981 tttgcccaga aagtatgtgt cacagcgata aagtcgactt cgggcctccc tcataaaaac
     2041 tggcagctct gaggtgaaca cctaaatcga atcgattcat tagaaagtta gtaaattatt
     2101 gaaatgcaaa tgtattctaa acatgactta catttatcgt ggcaaagacg ttttgaaagg
     2161 tcatgttggt caggaagagg aagatggctc cgttgatatt catcacaccc acttgcgtga
     2221 gttgttggcc caaaaagatg aggccaatca agatggcaac catctgcaaa ttaaaatgtt
     2281 actcgcatct cattaatatt cgcgagttaa atgaaattta tttatcttct gcaaaactat
     2341 aaactataca tctcattgaa aaaaactaag aagggtgtgg aatcaggcaa ttctatctaa
     2401 aatctagcga atttgtttcc aagaattgta agcgttatat catttgtttc cactggaacc
     2461 actcaccgtt gtctgaataa gtcgcacttt tacgaggagt ggttccttga gcaccgacag
     2521 ccaggatcgc cacaggaccg cccggaactg catgaaccag gtggccttgt aggtgtaccc
     2581 attctccggc tgctccagtg gcttctccag atttttggtg gccaacaact gctccatatc
     2641 ccgggctact ttgctaatgg caaaattgtc gcatatcttg gcgatccgat cacgggactc
     2701 gatctcccgt ccgggcacaa cggccaacac ctgtacgtaa aagtccgccg gattgtagtt
     2761 ggtaggacac tgggcaccca cgctggatag gagttgagat gttatgtaat actagatacc
     2821 cttaataaac acatcgaact cactaggaaa agaagtcgac ggcttcgctg ggagtgccca
     2881 agaaagctac cctgccctcg gccatcagaa ggatcttgtc aaagagctca aacagctcgg
     2941 aagacggctg atgaatggtc aggatgacgg tcttgccctt ctgcgacagc ttcttcagca
     3001 cctggacgac gctgtgggcg gtaaaggagt ccagtccgga ggtgggctca tcgcagatca
     3061 gaagcggcgg atcggttaga gcctcggagg cgaatgccag acgcttcctt tctccgccgg
     3121 acagaccttt caccctgccg ggcacaccga tgatcgtgtg ctgacatttg ctgagcgaaa
     3181 gctcctggat cacctgatcc acgcgggcca ctcgctgccg ataggtcaga tgtcgtggca
     3241 tccgcaccat ggcttggaaa atcaggtgtt ccctggccgt tagggagccg ataaagaggt
     3301 catcctgctg gacataggcg cacctggcct gcatctcctt ggcgtccaca ggttggccat
     3361 tgagcagtcg catcccggat ggcgatactt ggatgccctg cggcgatcga aaggcaaggg
     3421 cattcagcag ggtcgtcttt ccggcaccgg aactgcccat cacggccaaa agttcgcccg
     3481 gataggccac gccgcaaact gagtttcaaa ttggtaattg gaccctttat taagatttca
     3541 cacagatcag ccgactgcga atagaaactc accgttcttg agcaaatgtt tcctgggcgc
     3601 cggtatgtgt cgctcgttgc agaatagtcc gcgtgtccgg ttgaccagct gccgccatcc
     3661 ggagcccggc tgattgaccg ccccaaagat gtccatattg tgccaggcat aggtgaggtt
     3721 ctcggctagt tggccgctcc ctgaaccgga gtcctccggc ggactgggtg gccggagcgt
     3781 gccgtagttt ttggcctgcc cgaagccctg gttaatgcag ctctgcgaag ccgctccgct
     3841 gtcaccctgc aatgataggg gatctcaaat atcaactaca agcgttatgc tcatctaacc
     3901 ccgaacaaaa agtaccccga agtatcctac gaagtaggtt tatactttta tttatttttt
     3961 gtgcatctag gatcagctta aaatatctgg ttgttatatt ttttgtaaaa aagaatatag
     4021 tcgaaaatga atgcctttag atgtcttgat catgatatga tctcaaaaat tgtcttatat
     4081 agcgagaaca gctaccagaa taatctgttt cgtgtcacta tttgtttgtg caattgcggt
     4141 ttgggatttt tgtgggtcgc agttctcacg ccgcagacaa tttgatgttg caatcgcagt
     4201 tcctatagat caagtgaact taagatgtat gcacatgtac tactcacatt gttcagatgc
     4261 tcggcagatg ggtgtttgct gcctccgcga attaatagct cctgatcctc ttggcccatt
     4321 gccgggattt ttcacacttt cccctgctta cccacccaaa accaatcacc accccaatca
     4381 ctcaaaaaac aaacaaaaat aagaagcgag aggagttttg gcacagcact ttgtgtttaa
     4441 ttgatggcgt aaaccgcttg gagcttcgtc acgaaaccgc tgacaaaatg caactgaagg
     4501 cggacattga cgctacgtaa cgctacaaac ggtggcgaaa gagatagcgg acgcagcggc
     4561 gaaagagacg gcgatatttc tgtggacaga gaaggaggca aacagcgctg actttgagtg
     4621 gaatgtcatt ttgagtgaga ggtaatcgaa agaacctggt acatcaaata cccttggatc
     4681 gaagtaaatt taaaactgat cagataagtt caatgatatc cagtgcagta aaaaaaaaaa
     4741 atgttttttt tatctacttt ccgcaaaaat gggttttatt aacttacata catactaggc
     4801 gcgccagatc gcaagaagct tgatatcatc gatctcgagg ctgcatccaa cgcgttggga
     4861 gctctccgga tcaattcggc ttcaggtacc gtcgacgatg taggtcacgg tctcgaagcc
     4921 gcggtgcggg tgccagggcg tgcccttggg ctccccgggc gcgtactcca cctcacccat
     4981 ctggtccatc atgatgaacg ggtcgaggtg gcggtagttg atcccggcga acgcgcggcg
     5041 caccgggaag ccctcgccct cgaaaccgct gggcgcggtg gtcacggtga gcacgggacg
     5101 tgcgacggcg tcggctggtg cggatacgcg gggcagcgtc agcgggttct cgacggtcac
     5161 ggcgggcatg tcgacaagcc gaattgatcc actagaaggc ctaattcggt acactagttg
     5221 gccacgtaat aagtgtgcgt tgaatttatt cgcaaaaaca ttgcatattt tcggcaaagt
     5281 aaaattttgt tgcatacctt atcaaaaaat aagtgctgca tactttttag agaaaccaaa
     5341 taatttttta ttgcataccc gtttttaata aaatacattg cataccctct tttaataaaa
     5401 aatattgcat actttgacga aacaaatttt cgttgcatac ccaataaaag attattatat
     5461 tgcatacccg tttttaataa aatacattgc ataccctctt ttaataaaga atattgcata
     5521 cgttgacgaa acaaattttc gttgcatacc caataaaaga ttattatatt gcataccttt
     5581 tcttgccata ccatttagcc gatcaattgt gctcggcaac agtatatttg tggtgtgcca
     5641 accaacaaca ctagtagtac cagcttataa cttcgtataa tgtatgctat acgaagttat
     5701 ctgcaggcag gtcggagtac tgtcctccga gcggagtact gtcctccgag cggagtactg
     5761 tcctccgagc ggagtactgt cctccgagcg gagtactgtc ctccgagcgg agactcccat
     5821 ggataacttc gtataatgta tgctatacga agttatggat ccgcaggtcg gagtactgtc
     5881 ctccgagcgg agtactgtcc tccgagcgga gtactgtcct ccgagcggag tactgtcctc
     5941 cgagcggagt actgtcctcc gagcggagac tcgtcgacag cgagcgccgg agtataaata
     6001 gaggcgcttc gtctacggag cgacaattca attcaaacaa gcaaagtgaa cacgtcgcta
     6061 agcgaaagct aagcaaataa acaagcgcag ctgaacaagc taaacaatct gcagtaaagt
     6121 gcaagttaaa gtgaatcaat taaaagtaac cagcaaccaa gtaaatcaac tgcaactact
     6181 gaaatctgcc aagaagtaat tattgaatac aagaagagaa ctctgaatag ggaattggga
     6241 attcgcagat ctgcggccgc ggctcgaatc acaagtttgt acaaaaaagc aggctccgcg
     6301 gccgccccct tcaccatgga tatcagctat atcttcgtca tctgcctgat ggccctgtgc
     6361 agcggcggca gcagtctcag ccaagtcgag ggcaaacaga aatccggaag gggccggggc
     6421 tccatgtggt atccgtacga cgttcctgac tacgccgggg cccaaccagc gagatcgggt
     6481 ggcggaaagc ccattccgaa tcctttgtta ggcctggact ccacggcctc ggccattggg
     6541 cctgtggccg atttgcacat cgccaatgcc gacatatcgc cggacggatt caccagatcc
     6601 gcggtgctcg ctgggggcac atttccggga ccccttatca cgggcaacaa gggcgacaac
     6661 ttccgcatca atgtcattaa tcaattgacc gacaccacca tgctgcgaag cacaagtatc
     6721 cactggcatg gcttcttcca gcacggcacc agctgggccg atggtcccgc cttcgtaacc
     6781 cagtgcccga tagctcctgg caattcattt ctgtacaatt tcaccgtccc cgaccaggcg
     6841 ggcaccttct ggtaccacag ccatctgtcg actcagtact gcgatggtct cagcggacca
     6901 tttgtggtct atgatccgaa cgatccccat aggagcctct acgacgtgga cgatgagtcc
     6961 accgttataa cgctggccga ttggtatcac actccagcac cgcagctggc tgctgctccg
     7021 ccggtcccag atagtacact aataaacggc aagggacgct atgcaggcgg tccaacttca
     7081 ccattggccg tcatcaacgt ggagcagggc aaacgttatc gcttccgact catatccata
     7141 agctgcgacc caaactttac cttttccatt gatggccaca acatgaccat catcgaggcc
     7201 gatggagtaa acacccaacc tctgacggtg gacagcatcc agatcttcgc cggccagcgc
     7261 tactcgtttg tcctaaacgc caaccagccg gtaggcaatt actggatccg agcgaatccc
     7321 aatatcggca ctactggctt tgccggtggc attaacagtg ccatcctgcg gtatgtggga
     7381 gcgccggaag cggaccccac cactacacaa acaacgtcga caaatccagt caacgagacc
     7441 aatctgcatc cgctggagaa cccaggagca cccggaaacc ccacgcccgg aggtgcagat
     7501 gttaacatca acctcaacat tggattttcc tctacaggtc agttcacgat taatggcgtg
     7561 tccttcatcc ctcctactgt acccgtgctg ctgcagattt tgtcgggcgc ccgcaccgcg
     7621 caggatctgc ttccaagcgg aagtgtctac accctcccac cgaacaaagt tattgaaatc
     7681 agcatgccag ggggagcttc agcagcaggt ggtcctcatc ccttccattt acatggacac
     7741 acgtttgatg tggttaggtc tgctggctcg tcaacataca actacgtgaa tccagtgcgt
     7801 cgggatgtgg tttccattgg cacggctgga gacaatgtta cgattcgttt cacgacagat
     7861 aatcccggcc cctggttcct gcactgtcac attgactggc acctggaagc tggtttggca
     7921 gtggtgttcg cggaggatac gccggatatt gccagtgcga acccggtgcc agctgcctgg
     7981 gacgaccttt gtcccatcta tgatgccctg tcgcccgatg atcaaagcgg ccgcaaagag
     8041 attacgaatg ccttggaaac ctggggtgcc ttgggtcagg acatcaactt ggacattcct
     8101 agttttcaaa tgagtgatga tattgacgat ataaaatggg aaaaaacttc agacaagaaa
     8161 aagattgcac aattcagaaa agagaaagag actttcaagg aaaaagatac atataagcta
     8221 tttaaaaatg gaactctgaa aattaagcat ctgaagaccg atgatcagga tatctacaag
     8281 gtatcaatat atgatacaaa aggaaaaaat gtgttggaaa aaatatttga tttgaagatt
     8341 caagagaggg tctcaaaacc aaagatctcc tggacttgta tcaacacaac cctgacctgt
     8401 gaggtaatga atggaactga ccccgaatta aacctgtatc aagatgggaa acatctaaaa
     8461 ctttctcaga gggtcatcac acacaagtgg accaccagcc tgagtgcaaa attcaagtgc
     8521 acagcaggga acaaagtcag caaggaatcc agtgtcgagc ctgtcagctg tccagagaaa
     8581 ggtctggaca tctatctcat cattggcata tgtggaggag gcagcctctt gatggtcttt
     8641 gtggcactgc tcgttttcta tatcaccaaa aggaaaaaac gacgtcggat ccccgcagcc
     8701 gcagctgctg cggctgcggt ttgatagaag ggtgggcgcg ccgacccagc tttcttgtac
     8761 aaagtggtga ttcgagggta cctctagagc aaactagttc tgatctgcta gacaattgtt
     8821 ggcatcaggt aggcatcaca cacgattaac aacccctaaa aatacacttt gaaaatattg
     8881 aaaatatgtt tttgtataca tttttgatat tttcaaataa tacgcagtta taaaactcat
     8941 tagctaaccc attttttctt tgcttatgct tacagattgc aaagaactag agccgcggga
     9001 tctttgtgaa ggaaccttac ttctgtggtg tgacataatt ggacaaacta cctacagaga
     9061 tttaaagctc taaggtaaat ataaaatttt taagtgtata atgtgttaaa ctactgattc
     9121 taattgtttg tgtattttag attccaacct atggaactga tgaatgggag cagtggtgga
     9181 atgcctttaa tgaggaaaac ctgttttgct cagaagaaat gccatctagt gatgatgagg
     9241 ctactgctga ctctcaacat tctactcctc caaaaaagaa gagaaaggta gaagacccca
     9301 aggactttcc ttcagaattg ctaagttttt tgagtcatgc tgtgtttagt aatagaactc
     9361 ttgcttgctt tgctatttac accacaaagg aaaaagctgc actgctatac aagaaaatta
     9421 tggaaaaata tttgatgtat agtgccttga ctagagatca taatcagcca taccacattt
     9481 gtagaggttt tacttgcttt aaaaaacctc ccacacctcc ccctgaacct gaaacataaa
     9541 atgaatggaa ttgttgttgt taacttgttt attgcagctt ataatggtta caaataaagc
     9601 aatagcatca caaatttcac aaataaagca tttttttcac tgcattctag ttgtggtttg
     9661 tccaaactca tcaatgtatc ttatcatgtc tggttccaga gctctggcca cgtaataagt
     9721 gtgcgttgaa tttattcgca aaaacattgc atattttcgg caaagtaaaa ttttgttgca
     9781 taccttatca aaaaataagt gctgcatact ttttagagaa accaaataat tttttattgc
     9841 atacccgttt ttaataaaat acattgcata ccctctttta ataaaaaata ttgcatactt
     9901 tgacgaaaca aattttcgtt gcatacccaa taaaagatta ttatattgca tacccgtttt
     9961 taataaaata cattgcatac cctcttttaa taaagaatat tgcatacgtt gacgaaacaa
    10021 attttcgttg catacccaat aaaagattat tatattgcat accttttctt gccataccat
    10081 ttagccgatc aattgtgctc ggcaacagta tatttgtggt gtgccaacca acaacgagct
    10141 ccagcttttg ttccctttag tgagggttaa ttgcgcgctt ggcgtaatca tggtcatagc
    10201 tgtttcctgt gtgaaattgt tatccgctca caattccaca caacatacga gccggaagca
    10261 taaagtgtaa agcctggggt gcctaatgag tgagctaact cacattaatt gcgttgcgct
    10321 cactgcccgc tttccagtcg ggaaacctgt cgtgccagct gcattaatga atcggccaac
    10381 gcgcggggag aggcggtttg cgtattgggc gctcttccgc ttcctcgctc actgactcgc
    10441 tgcgctcggt cgttcggctg cggcgagcgg tatcagctca ctcaaaggcg gtaatacggt
    10501 tatccacaga atcaggggat aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg
    10561 ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca taggctccgc ccccctgacg
    10621 agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga ctataaagat
    10681 accaggcgtt tccccctgga agctccctcg tgcgctctcc tgttccgacc ctgccgctta
    10741 ccggatacct gtccgccttt ctcccttcgg gaagcgtggc gctttctcat agctcacgct
    10801 gtaggtatct cagttcggtg taggtcgttc gctccaagct gggctgtgtg cacgaacccc
    10861 ccgttcagcc cgaccgctgc gccttatccg gtaactatcg tcttgagtcc aacccggtaa
    10921 gacacgactt atcgccactg gcagcagcca ctggtaacag gattagcaga gcgaggtatg
    10981 taggcggtgc tacagagttc ttgaagtggt ggcctaacta cggctacact agaagaacag
    11041 tatttggtat ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt
    11101 gatccggcaa acaaaccacc gctggtagcg gtggtttttt tgtttgcaag cagcagatta
    11161 cgcgcagaaa aaaaggatct caagaagatc ctttgatctt ttctacgggg tctgacgctc
    11221 agtggaacga aaactcacgt taagggattt tggtcatgag attatcaaaa aggatcttca
    11281 cctagatcct tttaaattaa aaatgaagtt ttaaatcaat ctaaagtata tatgagtaaa
    11341 cttggtctga cagttaccaa tgcttaatca gtgaggcacc tatctcagcg atctgtctat
    11401 ttcgttcatc catagttgcc tgactccccg tcgtgtagat aactacgata cgggagggct
    11461 taccatctgg ccccagtgct gcaatgatac cgcgagaccc acgctcaccg gctccagatt
    11521 tatcagcaat aaaccagcca gccggaaggg ccgagcgcag aagtggtcct gcaactttat
    11581 ccgcctccat ccagtctatt aattgttgcc gggaagctag agtaagtagt tcgccagtta
    11641 atagtttgcg caacgttgtt gccattgcta caggcatcgt ggtgtcacgc tcgtcgtttg
    11701 gtatggcttc attcagctcc ggttcccaac gatcaaggcg agttacatga tcccccatgt
    11761 tgtgcaaaaa agcggttagc tccttcggtc ctccgatcgt tgtcagaagt aagttggccg
    11821 cagtgttatc actcatggtt atggcagcac tgcataattc tcttactgtc atgccatccg
    11881 taagatgctt ttctgtgact ggtgagtact caaccaagtc attctgagaa tagtgtatgc
    11941 ggcgaccgag ttgctcttgc ccggcgtcaa tacgggataa taccgcgcca catagcagaa
    12001 ctttaaaagt gctcatcatt ggaaaacgtt cttcggggcg aaaactctca aggatcttac
    12061 cgctgttgag atccagttcg atgtaaccca ctcgtgcacc caactgatct tcagcatctt
    12121 ttactttcac cagcgtttct gggtgagcaa aaacaggaag gcaaaatgcc gcaaaaaagg
    12181 gaataagggc gacacggaaa tgttgaatac tcatactctt cctttttcaa tattattgaa
    12241 gcatttatca gggttattgt ctcatgagcg gatacatatt tgaatgtatt tagaaaaata
    12301 aacaaatagg ggttccgcgc acatttcccc gaaaagtgcc acctg
//
