LOCUS       sequence-464629-        9550 bp    DNA     circular SYN 15-APR-2025
DEFINITION  expresses LaccID in the Golgi apparatus, dox-inducible lentiviral 
            vector.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9550)
  TITLE     Directed evolution of LaccID for cell surface proximity labeling and
            EM
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 9550)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pTRE3G_ManII-LaccID-V5_Puro
FEATURES             Location/Qualifiers
     source          1..9550
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        5..231
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             232..412
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    459..584
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1077..1310
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1495..1539
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    1752..1869
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     protein_bind    1927..1945
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    1963..1981
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    1999..2017
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2035..2053
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2071..2089
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2107..2125
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2143..2161
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     2206..2230
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     CDS             4103..4144
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     misc_feature    4161..4749
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(4214..4234)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(4632..4643)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     promoter        4769..5279
                     /label=hPGK promoter
                     /note="human phosphoglycerate kinase 1 promoter"
     primer_bind     5198..5216
                     /label=hPGK-F
                     /note="Human PGK promoter, forward primer"
     CDS             5289..6032
                     /codon_start=1
                     /product="modified rtTA protein that binds tightly to 
                     promoters containing the tet operator in the presence of 
                     doxycycline"
                     /label=Tet-On(R) 3G
                     /translation="MSRLDKSKVINSALELLNGVGIEGLTTRKLAQKLGVEQPTLYWHV
                     KNKRALLDALPIEMLDRHHTHSCPLEGESWQDFLRNNAKSYRCALLSHRDGAKVHLGTR
                     PTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEEQEHQVAKEERETPTT
                     DSMPPLLKQAIELFDRQGAEPAFLFGLELIICGLEKQLKCESGGPTDALDDFDLDMLPA
                     DALDDFDLDMLPADALDDFDLDMLPG"
     CDS             6042..6098
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             6099..6695
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="TEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIERV
                     TELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLAA
                     QQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETSA
                     PRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     6592..6612
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     LTR             6794..7027
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    7099..7220
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7136..7155)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     7190..7209
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      7260..7395
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     promoter        7395..7466
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(7467..7535,7536..8327)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(7685..7704)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      8498..9086
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8987..9006
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     9240..9257
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    9374..9395
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(9410..9415,9416..9433,9434..9440)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    9448..9464
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     9453..9475
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     9472..9488
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     9472..9488
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     9507..9527
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        9509..9527
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
ORIGIN
        1 ttaatgtagt cttatgcaat actcttgtag tcttgcaaca tggtaacgat gagttagcaa
       61 catgccttac aaggagagaa aaagcaccgt gcatgccgat tggtggaagt aaggtggtac
      121 gatcgtgcct tattaggaag gcaacagacg ggtctgacat ggattggacg aaccactgaa
      181 ttgccgcatt gcagagatat tgtatttaag tgcctagctc gatacataaa cgggtctctc
      241 tggttagacc agatctgagc ctgggagctc tctggctaac tagggaaccc actgcttaag
      301 cctcaataaa gcttgccttg agtgcttcaa gtagtgtgtg cccgtctgtt gtgtgactct
      361 ggtaactaga gatccctcag acccttttag tcagtgtgga aaatctctag cagtggcgcc
      421 cgaacaggga cttgaaagcg aaagggaaac cagaggagct ctctcgacgc aggactcggc
      481 ttgctgaagc gcgcacggca agaggcgagg ggcggcgact ggtgagtacg ccaaaaattt
      541 tgactagcgg aggctagaag gagagagatg ggtgcgagag cgtcagtatt aagcggggga
      601 gaattagatc gcgatgggaa aaaattcggt taaggccagg gggaaagaaa aaatataaat
      661 taaaacatat agtatgggca agcagggagc tagaacgatt cgcagttaat cctggcctgt
      721 tagaaacatc agaaggctgt agacaaatac tgggacagct acaaccatcc cttcagacag
      781 gatcagaaga acttagatca ttatataata cagtagcaac cctctattgt gtgcatcaaa
      841 ggatagagat aaaagacacc aaggaagctt tagacaagat agaggaagag caaaacaaaa
      901 gtaagaccac cgcacagcaa gcggccgctg atcttcagac ctggaggagg agatatgagg
      961 gacaattgga gaagtgaatt atataaatat aaagtagtaa aaattgaacc attaggagta
     1021 gcacccacca aggcaaagag aagagtggtg cagagagaaa aaagagcagt gggaatagga
     1081 gctttgttcc ttgggttctt gggagcagca ggaagcacta tgggcgcagc gtcaatgacg
     1141 ctgacggtac aggccagaca attattgtct ggtatagtgc agcagcagaa caatttgctg
     1201 agggctattg aggcgcaaca gcatctgttg caactcacag tctggggcat caagcagctc
     1261 caggcaagaa tcctggctgt ggaaagatac ctaaaggatc aacagctcct ggggatttgg
     1321 ggttgctctg gaaaactcat ttgcaccact gctgtgcctt ggaatgctag ttggagtaat
     1381 aaatctctgg aacagatttg gaatcacacg acctggatgg agtgggacag agaaattaac
     1441 aattacacaa gcttaataca ctccttaatt gaagaatcgc aaaaccagca agaaaagaat
     1501 gaacaagaat tattggaatt agataaatgg gcaagtttgt ggaattggtt taacataaca
     1561 aattggctgt ggtatataaa attattcata atgatagtag gaggcttggt aggtttaaga
     1621 atagtttttg ctgtactttc tatagtgaat agagttaggc agggatattc accattatcg
     1681 tttcagaccc acctcccaac cccgagggga caattctcga cctcgagaca aatggcagta
     1741 ttcatccaca attttaaaag aaaagggggg attggggggt acagtgcagg ggaaagaata
     1801 gtagacataa tagcaacaga catacaaact aaagaattac aaaaacaaat tacaaaaatt
     1861 caaaattttc gggtttatta cagggacagc agagatccac tttggccgcg aatcgatatg
     1921 ttttactccc tatcagtgat agagaacgta tgaagagttt actccctatc agtgatagag
     1981 aacgtatgca gactttactc cctatcagtg atagagaacg tataaggagt ttactcccta
     2041 tcagtgatag agaacgtatg accagtttac tccctatcag tgatagagaa cgtatctaca
     2101 gtttactccc tatcagtgat agagaacgta tatccagttt actccctatc agtgatagag
     2161 aacgtataag ctttaggcgt gtacggtggg cgcctataaa agcagagctc gtttagtgaa
     2221 ccgtcagatc gcctggagca attccacaac acttttgtct tataccaact ttccgtacca
     2281 cttcctaccc tcgtaaatag agctagccac catgaagtta agtcgccagt tcaccgtgtt
     2341 tggcagcgcg atcttctgcg tcgtaatctt ctcactctac ctgatgctgg acaggggtca
     2401 cttggactac cctcggggcc cgcgccagga gggctccttt ccgcagggcc agctttcaat
     2461 attgcaagaa aagattgacc atttggagcg tttgctcgct gagaacaacg agatcatctc
     2521 aaatatcaga gactcagtca tcaacctgag cgagtctgtg gaggacggcc cgcgggggtc
     2581 accaggcaac gccagccaag gcacgcgtgc gataggacct gtggcagacc ttcacatcgc
     2641 caatgcagac atcagcccgg atgggttcac tcgttccgct gtgctagcgg gtggaacatt
     2701 tcccggtccc ttaataacgg gtaataaagg agataatttc cggataaacg tcatcaacca
     2761 gcttacggac acgactatgt tgaggtcaac cagtatacat tggcacggat tctttcaaca
     2821 cgggacaagt tgggctgacg gccccgcttt cgtcactcag tgccccatcg caccgggtaa
     2881 cagttttctt tacaacttca ctgtcccgga tcaagcgggc acgttctggt atcatagtca
     2941 cctaagtacc cagtattgtg acggacttag tggtccgttt gtagtttacg accccaacga
     3001 tccccatcgt agcttatacg atgtggatga tgaaagcact gtaattactc ttgcggactg
     3061 gtaccacacc ccggctcccc agttggccgc tgcgccgccc gtcccagata gcaccctaat
     3121 aaacgggaaa ggaaggtatg ccggaggccc aacgtccccg ttagccgtga taaacgtaga
     3181 gcagggaaaa agatataggt ttcgtcttat atctatttcc tgtgatccga attttacatt
     3241 tagcattgat ggccataata tgactatcat tgaagctgat ggggtgaaca cgcaaccact
     3301 gaccgtggac tctatccaga ttttcgctgg ccaaagatat tccttcgtcc tgaatgcgaa
     3361 ccagcctgtt ggtaactatt ggataagagc gaatccaaat ataggaacga caggattcgc
     3421 aggtggtatt aatagtgcga tcctacgtta tgtaggagct ccagaagcag accctacaac
     3481 gacccaaaca acatccacaa atccagtgaa cgaaacaaac ctacacccct tggagaaccc
     3541 cggagccccg gggaatccga ctccgggtgg tgctgatgtg aacatcaacc ttaacatcgg
     3601 attctcctca acgggacaat tcaccataaa cggtgtttcc ttcataccac ccaccgttcc
     3661 cgtcttgttg caaatcctgt ccggggcgcg tacggcacaa gatctgcttc ctagcggctc
     3721 tgtttataca ttaccgccga acaaagtcat agaaattagt atgccaggcg gcgctagcgc
     3781 agccgggggg cctcacccgt tccatttgca cggccacact tttgatgtgg tacgttcagc
     3841 gggatcatca acttacaact acgtgaatcc agtcagaaga gatgtcgtct ccattggaac
     3901 ggcgggagat aacgtgacaa ttaggttcac tacggacaac ccgggtccat ggttcctaca
     3961 ttgtcatatc gactggcatc ttgaggccgg tttagccgtc gtattcgctg aagacacacc
     4021 tgatatcgca agcgctaatc ccgttccggc tgcgtgggac gacctatgcc ctatttatga
     4081 tgcgttaagc cccgacgatc aaggcaagcc catccccaac cccctgctgg gcctggacag
     4141 cacctgataa ggccgaattc aatcaacctc tggattacaa aatttgtgaa agattgactg
     4201 gtattcttaa ctatgttgct ccttttacgc tatgtggata cgctgcttta atgcctttgt
     4261 atcatgctat tgcttcccgt atggctttca ttttctcctc cttgtataaa tcctggttgc
     4321 tgtctcttta tgaggagttg tggcccgttg tcaggcaacg tggcgtggtg tgcactgtgt
     4381 ttgctgacgc aacccccact ggttggggca ttgccaccac ctgtcagctc ctttccggga
     4441 ctttcgcttt ccccctccct attgccacgg cggaactcat cgccgcctgc cttgcccgct
     4501 gctggacagg ggctcggctg ttgggcactg acaattccgt ggtgttgtcg gggaaatcat
     4561 cgtcctttcc ttggctgctc gcctgtgttg ccacctggat tctgcgcggg acgtccttct
     4621 gctacgtccc ttcggccctc aatccagcgg accttccttc ccgcggcctg ctgccggctc
     4681 tgcggcctct tccgcgtctt cgccttcgcc ctcagacgag tcggatctcc ctttgggccg
     4741 cctccccgcc tgtctgcaga tatcatccgg ggttggggtt gcgccttttc caaggcagcc
     4801 ctgggtttgc gcagggacgc ggctgctctg ggcgtggttc cgggaaacgc agcggcgccg
     4861 accctgggtc tcgcacattc ttcacgtccg ttcgcagcgt cacccggatc ttcgccgcta
     4921 cccttgtggg ccccccggcg acgcttcctg ctccgcccct aagtcgggaa ggttccttgc
     4981 ggttcgcggc gtgccggacg tgacaaacgg aagccgcacg tctcactagt accctcgcag
     5041 acggacagcg ccagggagca atggcagcgc gccgaccgcg atgggctgtg gccaatagcg
     5101 gctgctcagc agggcgcgcc gagagcagcg gccgggaagg ggcggtgcgg gaggcggggt
     5161 gtggggcggt agtgtgggcc ctgttcctgc ccgcgcggtg ttccgcattc tgcaagcctc
     5221 cggagcgcac gtcggcagtc ggctccctcg ttgaccgaat caccgacctc tctccccagc
     5281 aattcaccat gtctagactg gacaagagca aagtcataaa ctctgctctg gaattactca
     5341 atggagtcgg tatcgaaggc ctgacgacaa ggaaactcgc tcaaaagctg ggagttgagc
     5401 agcctaccct gtactggcac gtgaagaaca agcgggccct gctcgatgcc ctgccaatcg
     5461 agatgctgga caggcatcat acccactcct gccccctgga aggcgagtca tggcaagact
     5521 ttctgcggaa caacgccaag tcataccgct gtgctctcct ctcacatcgc gacggggcta
     5581 aagtgcatct cggcacccgc ccaacagaga aacagtacga aaccctggaa aatcagctcg
     5641 cgttcctgtg tcagcaaggc ttctccctgg agaacgcact gtacgctctg tccgccgtgg
     5701 gccactttac actgggctgc gtattggagg aacaggagca tcaagtagca aaagaggaaa
     5761 gagagacacc taccaccgat tctatgcccc cacttctgaa acaagcaatt gagctgttcg
     5821 accggcaggg agccgaacct gccttccttt tcggcctgga actaatcata tgtggcctgg
     5881 agaaacagct aaagtgcgaa agcggcgggc cgaccgacgc ccttgacgat tttgacttag
     5941 acatgctccc agccgatgcc cttgacgact ttgaccttga tatgctgcct gctgacgctc
     6001 ttgacgattt tgaccttgac atgctccccg ggggaagcgg agctactaac ttcagcctgc
     6061 tgaagcaggc tggagacgtg gaggagaacc ctggacctac cgagtacaag cccacggtgc
     6121 gcctcgccac ccgcgacgac gtcccccggg ccgtacgcac cctcgccgcc gcgttcgccg
     6181 actaccccgc cacgcgccac accgtcgacc cggaccgcca catcgagcgg gtcaccgagc
     6241 tgcaagaact cttcctcacg cgcgtcgggc tcgacatcgg caaggtgtgg gtcgcggacg
     6301 acggcgccgc ggtggcggtc tggaccacgc cggagagcgt cgaagcgggg gcggtgttcg
     6361 ccgagatcgg cccgcgcatg gccgagttga gcggttcccg gctggccgcg cagcaacaga
     6421 tggaaggcct cctggcgccg caccggccca aggagcccgc gtggttcctg gccaccgtcg
     6481 gcgtctcgcc cgaccaccag ggcaagggtc tgggcagcgc cgtcgtgctc cccggagtgg
     6541 aggcggccga gcgcgccggg gtgcccgcct tcctggagac ctccgcgccc cgcaacctcc
     6601 ccttctacga gcggctcggc ttcaccgtca ccgccgacgt cgaggtgccc gaaggaccgc
     6661 gcacctggtg catgacccgc aagcccggtg cctaactgtt tcgcctcggc gtccggacta
     6721 gaggtacctt taagaccaat gacttacaag gcagctgtag atcttagcca ctttttaaaa
     6781 gaaaaggggg gactggaagg gctaattcac tcccaacgaa gacaagatct gctttttgct
     6841 tgtactgggt ctctctggtt agaccagatc tgagcctggg agctctctgg ctaactaggg
     6901 aacccactgc ttaagcctca ataaagcttg ccttgagtgc ttcaagtagt gtgtgcccgt
     6961 ctgttgtgtg actctggtaa ctagagatcc ctcagaccct tttagtcagt gtggaaaatc
     7021 tctagcagta gtagttcatg tcatcttatt attcagtatt tataacttgc aaagaaatga
     7081 atatcagaga gtgagaggaa cttgtttatt gcagcttata atggttacaa ataaagcaat
     7141 agcatcacaa atttcacaaa taaagcattt ttttcactgc attctagttg tggtttgtcc
     7201 aaactcatca atgtatctta tcatgtctgg ctctagctat cccgccccta actccgccca
     7261 tcccgcccct aactccgccc agttccgccc attctccgcc ccatggctga ctaatttttt
     7321 ttatttatgc agaggccgag gccgcctcgg cctctgagct attccagaag tagtgaggag
     7381 gcttttttgg aggcctttca aatatgtatc cgctcatgag acaataaccc tgataaatgc
     7441 ttcaataata ttgaaaaagg aagagtatga gtattcaaca tttccgtgtc gcccttattc
     7501 ccttttttgc ggcattttgc cttcctgttt ttgctcaccc agaaacgctg gtgaaagtaa
     7561 aagatgctga agatcagttg ggtgcacgag tgggttacat cgaactggat ctcaacagcg
     7621 gtaagatcct tgagagtttt cgccccgaag aacgttttcc aatgatgagc acttttaaag
     7681 ttctgctatg tggcgcggta ttatcccgta ttgacgccgg gcaagagcaa ctcggtcgcc
     7741 gcatacacta ttctcagaat gacttggttg agtactcacc agtcacagaa aagcatctta
     7801 cggatggcat gacagtaaga gaattatgca gtgctgccat aaccatgagt gataacactg
     7861 cggccaactt acttctgaca acgatcggag gaccgaagga gctaaccgct tttttgcaca
     7921 acatggggga tcatgtaact cgccttgatc gttgggaacc ggagctgaat gaagccatac
     7981 caaacgacga gcgtgacacc acgatgcctg tagcaatggc aacaacgttg cgcaaactat
     8041 taactggcga actacttact ctagcttccc ggcaacaatt aatagactgg atggaggcgg
     8101 ataaagttgc aggaccactt ctgcgctcgg cccttccggc tggctggttt attgctgata
     8161 aatctggagc cggtgagcgt gggtctcgcg gtatcattgc agcactgggg ccagatggta
     8221 agccctcccg tatcgtagtt atctacacga cggggagtca ggcaactatg gatgaacgaa
     8281 atagacagat cgctgagata ggtgcctcac tgattaagca ttggtaactg tcagaccaag
     8341 tttactcata tatactttag attgatttaa aacttcattt ttaatttaaa aggatctagg
     8401 tgaagatcct ttttgataat ctcatgacca aaatccctta acgtgagttt tcgttccact
     8461 gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg
     8521 taatctgctg cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc
     8581 aagagctacc aactcttttt ccgaaggtaa ctggcttcag cagagcgcag ataccaaata
     8641 ctgttcttct agtgtagccg tagttaggcc accacttcaa gaactctgta gcaccgccta
     8701 catacctcgc tctgctaatc ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc
     8761 ttaccgggtt ggactcaaga cgatagttac cggataaggc gcagcggtcg ggctgaacgg
     8821 ggggttcgtg cacacagccc agcttggagc gaacgaccta caccgaactg agatacctac
     8881 agcgtgagct atgagaaagc gccacgcttc ccgaagggag aaaggcggac aggtatccgg
     8941 taagcggcag ggtcggaaca ggagagcgca cgagggagct tccaggggga aacgcctggt
     9001 atctttatag tcctgtcggg tttcgccacc tctgacttga gcgtcgattt ttgtgatgct
     9061 cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc ggccttttta cggttcctgg
     9121 ccttttgctg gccttttgct cacatgttct ttcctgcgtt atcccctgat tctgtggata
     9181 accgtattac cgcctttgag tgagctgata ccgctcgccg cagccgaacg accgagcgca
     9241 gcgagtcagt gagcgaggaa gcggaagagc gcccaatacg caaaccgcct ctccccgcgc
     9301 gttggccgat tcattaatgc agctggcacg acaggtttcc cgactggaaa gcgggcagtg
     9361 agcgcaacgc aattaatgtg agttagctca ctcattaggc accccaggct ttacacttta
     9421 tgcttccggc tcgtatgttg tgtggaattg tgagcggata acaatttcac acaggaaaca
     9481 gctatgacca tgattacgcc aagcgcgcaa ttaaccctca ctaaagggaa caaaagctgg
     9541 agctgcaagc
//
